Guinea grass Na+/H+ reversal transportation protein gene sequence and its clone and application

Abstract

The invention discloses a gene sequence of Aneurolepidiumchinense Na+ / H+ antiport protein, a clone and application thereof, belonging to the molecular biology field. A coded gene of vacuole type Na+ / H+ antiport protein is separated from the Aneurolepidiumchinense for the first time with RT-PCR through designing a primer according to an amino acid sequence of the Na+ / H+ antipor protein published in other plants. With the transfer of the gene, the anti-salt property of a salt-sensitive yeast mutant can be recovered to a certain degree. A transgenic plant has higher salt tolerance which can reach 200mM through further constructing a sense expression vector and transforming tobacco. The gene can be used in genetic transformation of a plant to improve the salt tolerance of the plant.

Description

(1) Technical field

[0001] The present invention relates to Na in Leymus chinense (Aneurolepidium chinense) + / H + The cloning, recombination and salt tolerance function analysis and application of the antiporter gene AcNHX1 belong to the field of molecular biology and biotechnology. (2) Background technology

[0002] High concentrations of salt cause ion imbalance and hyperosmotic stress in plants, resulting in poor plant development, slow growth and especially reduced crop yields. Severe salt stress or osmotic stress will cause the second stress in plants - oxidative stress, and even cause plant death. Therefore, people pay more and more attention to salt stress, and people pay more and more attention to improving the salt tolerance of crops. Under high salinity, plants mitigate the toxicity caused by salt stress by producing stress proteins and soluble osmoregulatory substances. Early salt-tolerant genetic engineering mainly focused on scavenging free radicals and inc...

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