Cadaverine-producing engineering strain

A technology of engineering bacteria and genetically engineered bacteria, which is applied in the direction of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems of low cadaverine synthesis, inability to transport cadaverine, inability to synthesize lysine in large quantities, etc., and achieve a huge economy Benefits and practical benefits, broad market development prospects, the effect of curbing global warming

Inactive Publication Date: 2012-04-25
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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AI Technical Summary

Problems solved by technology

Although Escherichia coli, Bacillus cadaverium, Hafnia hives, etc. can directly synthesize cadaverine, and the regulation of cadaverine synthesis has been extensively studied, these bacteria cannot synthesize lysine in large quantities, and the synthesis of cadaverine is l...

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  • Cadaverine-producing engineering strain
  • Cadaverine-producing engineering strain
  • Cadaverine-producing engineering strain

Examples

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Embodiment Construction

[0033] Below in conjunction with the examples, the present invention is further described, the following examples are illustrative, not limiting, and the protection scope of the present invention cannot be limited by the following examples.

[0034] A cadaverine engineering bacterium, the host bacterium is Corynebacterium glutamicum (including but not limited to Corynebacterium glutamicum ATCC13032), which has a lysine decarboxylase gene and a lysine-cadaverine antiporter gene. The lysine decarboxylase gene and the lysine-cadaverine antiporter gene are heterologous to the engineering bacteria. The lysine decarboxylase gene is derived from Hafnia alvei. The lysine-cadaverine antiporter gene is derived from Escherichia coli. The size of the lysine decarboxylase gene is 2220bp, and its sequence is sequence 1; the lysine-cadaverine antiporter gene is 1335bp, and its sequence is sequence 4.

[0035] Wherein, the lysine decarboxylase gene and the lysine-cadaverine antiporter gene ...

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Abstract

The invention relates to a cadaverine-producing engineering strain, a construction method thereof and a method for producing cadaverine by using the strain. Based on a gene engineering technology, an L-lysine decarboxylase gene (cadA/LDC) and a cadaverine-lysine antiporter gene (cadB) are respectively or simultaneously cloned into Corynebacterium glutamicum and subjected to induced coexpression, the lysine is decarboxylated to generate cadaverine under the catalytic action of the CadA enzyme, and the cadaverine is transported out of cells by the CadB protein, thus constructing a high-yield cadaverine-producing engineering strain and establishing a novel one-step cadaverine synthesis method based on the fermentation of Corynebacterium glutamicum. The one-step cadaverine synthesis method based on microbial fermentation provides a new way for large-scale cadaverine production, thereby having great economic benefits and social benefits and wide market development prospects.

Description

technical field [0001] The invention belongs to the field of genetic engineering bacteria, in particular to a cadaverine-producing engineering bacteria. Background technique [0002] Cadaverine (Cadaverine) is a polyamine, that is, 1,5-pentanediamine (pentamethylenediamine for short), which is produced by decarboxylation of lysine in organisms, and is a biologically active nitrogen-containing compound widely present in organisms. Alkaline, but also present in spoilage as a carnitine. Cadaverine is an important raw material for the synthesis of new materials polyamide-54 (condensed from cadaverine and succinic acid) and polyamide-56 (condensed from cadaverine and oxalic acid), and has important industrial applications. [0003] Currently, there are two methods for synthesizing cadaverine: chemical synthesis and enzymatic conversion. The chemical synthesis method has harsh conditions and pollutes the environment, while the enzymatic conversion method has a complicated proces...

Claims

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Application Information

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IPC IPC(8): C12N1/21C12R1/15
Inventor 黎明路福平王春霞黄云雁刘萌
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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