Alfalfa Na+/H+ reverse transport protein gene and its clone and use

An antiporter, alfalfa technology, applied in the field of molecular biology and biology, can solve problems such as less obvious effect
CN1632122AInactive Publication Date: 2005-06-29SHANDONG AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANDONG AGRICULTURAL UNIVERSITY
Publication Date
2005-06-29
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the cloning, recombination, salt tolerance function analysis and application of alfalfa Na+ / H+ antiporter MsNHX1 gene, and belongs to the field of molecular biology and biotechnology. Total RNA was extracted from alfalfa roots, and then 1 μg of total RNA was reverse transcribed into cDNA. According to the conserved amino acid sequences of Na+ / H+ antiporters in other plants, a pair of degenerate primers were designed, and conventional polymerase chain reaction (PCR) was performed. The PCR product was connected to the pGEM-T vector and transformed into DH5α cells. Perform sequence determination. The full-length cDNA was then obtained by rapid amplification of the 3' and 5' ends. Further construct the sense expression vector and transform Arabidopsis. Transgenic Arabidopsis has higher salt tolerance. Therefore, the gene transformation of dicotyledonous crops such as alfalfa will improve their salt tolerance, yield and quality, thereby expanding the planting area of ​​dicotyledonous plants on saline-alkali land and generating huge economic and social benefits.
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Description

(1) Technical field:

[0001] The present invention relates to Na in alfalfa + / H + The cloning and recombination of the antiporter MsNHX1 gene and the analysis and application of salt tolerance function belong to the field of molecular biology and biotechnology. (two) background technology:

[0002] Salt stress can cause ion osmotic stress, generate active oxygen, destroy the osmotic potential of plants and the balance of ion distribution, resulting in plant damage, especially affecting the yield of crops. Therefore, improving the salt tolerance of crops has attracted more and more attention. Under high-salt conditions, plants can alleviate the toxicity caused by salt stress by producing stress proteins and soluble osmoregulatory substances. Early salt-tolerant genetic engineering mainly focused on scavenging free radicals and increasing osmotic adjustment substances. The salt-tolerant ability of transgenic plants was improved, but the effect was not obvious. Recently, Na...

Claims

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