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A kind of breeding method of sugar beet with strong salt tolerance and drought resistance

A sugar beet and seed technology, which is applied in the field of strong salt-tolerant and drought-resistant sugar beet cultivation, can solve the problems of increasing the risk of beet hybrid plant diseases and insect pests, improving sugar content resistance, and limiting gene utilization potential, etc., to achieve sugar content High yield, strong salt and drought resistance, high sugar content

Inactive Publication Date: 2018-04-27
LANZHOU UNIVERSITY OF TECHNOLOGY
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  • Application Information

AI Technical Summary

Problems solved by technology

At present, the traditional sugar beet breeding mainly selects inbred lines with good adaptability and high combining ability as hybrid parents, and uses the method of continuous forced selfing to breed inbred lines. The danger of encroachment, the close blood relationship between varieties greatly limits the potential of gene utilization, and thus becomes the main obstacle to increase sugar content and enhance stress resistance

Method used

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  • A kind of breeding method of sugar beet with strong salt tolerance and drought resistance
  • A kind of breeding method of sugar beet with strong salt tolerance and drought resistance
  • A kind of breeding method of sugar beet with strong salt tolerance and drought resistance

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Experimental program
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Effect test

Embodiment 1

[0026] Example 1: Agrobacterium-mediated wxya with ZxVP1-1 Genetically transformed sugar beets, see Figure 1~Figure 6 :

[0027] Genetic transformation mediated by Agrobacterium tumefaciens is currently the most commonly used, most studied, and most mature method. As early as 1974, Zeanen et al. discovered that when Agrobacterium tumefaciens infects plants, a piece of DNA on its plasmid can be inserted into the plant genome, thereby causing changes in plant genetic characteristics. Transformation of genes into plant genomes provides the basis. Since then, people have used the natural vector system of Agrobacterium tumefaciens to infect specific plant cells, introduce the target gene into the genome, and obtain transgenic strains with the help of cell culture technology to achieve the purpose of genetic transformation of exogenous genes (Hood et al., 1993 ). At present, Agrobacterium tumefaciens has successfully mediated the genetic transformation of most dicotyledonous...

Embodiment 2

[0031] Embodiment 2: the PCR detection of the target gene of transgenic sugar beet line, see Figure 7-Figure 8 :

[0032] Agrobacterium-mediated genetic transformation of exogenous genes to plants, mainly through the contact of Agrobacterium liquid with the target gene with the plant germ cells, using the natural infection characteristics of Agrobacterium to transfer the exogenous target gene into the explanted plant body. However, whether the exogenous gene mediated by Agrobacterium can be successfully transformed into the plant requires transgenic plants to provide evidence at the molecular level, which is an indispensable step for gene transformation and the most effective means to determine whether the target gene has been transformed ( Wang Guanlin et al., 2002).

[0033] Isolation of high-yield and high-quality DNA and RNA from plants is not an easy task due to the interference of cell walls and substances unique to plant cells. Therefore, when selecting materials, w...

Embodiment 3

[0043] Example 3: Physiological analysis of new transgenic beet lines with high salt tolerance and sugar content, see Figure 9~Figure 21 :

[0044] High concentrations of salt can cause ionic stress and osmotic stress to plants, seriously affecting the normal physiological and biochemical reactions of plants, resulting in slow growth or even death of plants. Although the gene of interest was detected in step 2 wxya with ZxVP1-1 It has been integrated into the sugar beet genome and overexpressed. However, whether it can improve the yield, phenotypic traits and salt tolerance of sugar beet in a saline environment is the focus of most attention, and it is also the ultimate goal of the present invention. Therefore, the present invention selects growth potential identical, target gene wxya with ZxVP1-1 The transgenic (T0) and wild-type (WT) sugar beet lines with consistent expression were treated with 1 / 2 Hogland nutrient solution containing 400 mmol / L NaCl respectively, a...

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Abstract

A method for cultivating sugar beet with strong salt tolerance and drought resistance, the purpose of which is to avoid the defects of traditional breeding and the lack of previous techniques for single gene transformation, and realize the combination of two Na+ / H+ antiporter genes ZxNHX and vacuolar Membrane H+-pyrophosphatase gene ZxVP1-1 is polymerized, and a new transgenic sugar beet variety (line) co-expressing ZxNHX and ZxVP1-1 is obtained through genetic transformation, providing a method for cultivating sugar beets with strong salt tolerance and drought resistance and high sugar content; The steps include seedling cultivation, seedling infection, molecular identification and stress resistance evaluation. This method greatly shortens the cultivation time of transgenic seedlings, the whole cycle is controlled at about 5 months, no callus is required, and the genetic transformation efficiency is as high as 10%.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a method for cultivating sugar beets with strong salt tolerance and drought resistance. Background technique [0002] Drought and soil salinization are the two most important environmental factors limiting crop growth and yield (Zhu JK.2001. Plant Sci. 6: 66-71). According to statistics, my country is one of the countries with the most severe drought in the world, especially the arid and semi-arid regions in Northwest China account for 50.8% of the total land area of ​​my country (Shan Lun. 2005. Qiushi Magazine. 22: 42-43). Moreover, with the greenhouse CO 2 The global temperature is increasing year by year, causing some semi-humid lands to evolve into arid areas, and even leading to the extinction of some plants that were originally adapted to arid areas. In addition, due to the high temperature and dryness, the evaporation of soil moisture is strong, and due to inappropriate farmin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/84A01H5/00A01H6/02
Inventor 伍国强冯瑞军王锁民刘左军包爱科袁惠君李善家
Owner LANZHOU UNIVERSITY OF TECHNOLOGY
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