A kind of breeding method of sugar beet with strong salt tolerance and drought resistance

A sugar beet and seed technology, which is applied in the field of strong salt-tolerant and drought-resistant sugar beet cultivation, can solve the problems of increasing the risk of beet hybrid plant diseases and insect pests, improving sugar content resistance, and limiting gene utilization potential, etc., to achieve sugar content High yield, strong salt and drought resistance, high sugar content
CN104830899BInactive Publication Date: 2018-04-27LANZHOU UNIVERSITY OF TECHNOLOGY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
LANZHOU UNIVERSITY OF TECHNOLOGY
Publication Date
2018-04-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

A method for cultivating sugar beet with strong salt tolerance and drought resistance, the purpose of which is to avoid the defects of traditional breeding and the lack of previous techniques for single gene transformation, and realize the combination of two Na+ / H+ antiporter genes ZxNHX and vacuolar Membrane H+-pyrophosphatase gene ZxVP1-1 is polymerized, and a new transgenic sugar beet variety (line) co-expressing ZxNHX and ZxVP1-1 is obtained through genetic transformation, providing a method for cultivating sugar beets with strong salt tolerance and drought resistance and high sugar content; The steps include seedling cultivation, seedling infection, molecular identification and stress resistance evaluation. This method greatly shortens the cultivation time of transgenic seedlings, the whole cycle is controlled at about 5 months, no callus is required, and the genetic transformation efficiency is as high as 10%.
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Description

technical field

[0001] The invention belongs to the field of biotechnology and relates to a method for cultivating sugar beets with strong salt tolerance and drought resistance. Background technique

[0002] Drought and soil salinization are the two most important environmental factors limiting crop growth and yield (Zhu JK.2001. Plant Sci. 6: 66-71). According to statistics, my country is one of the countries with the most severe drought in the world, especially the arid and semi-arid regions in Northwest China account for 50.8% of the total land area of ​​my country (Shan Lun. 2005. Qiushi Magazine. 22: 42-43). Moreover, with the greenhouse CO 2 The global temperature is increasing year by year, causing some semi-humid lands to evolve into arid areas, and even leading to the extinction of some plants that were originally adapted to arid areas. In addition, due to the high temperature and dryness, the evaporation of soil moisture is strong, and due to inappropriate farmin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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