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Genetic loci associated with response to abiotic stress

A related, genetic technology, applied in genetic engineering, biochemical equipment and methods, applications, etc., can solve problems such as difficulty in cultivating salt tolerance

Active Publication Date: 2016-07-13
EI DU PONT DE NEMOURS & CO +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, salt tolerance is a complex trait that is difficult to breed in plants

Method used

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  • Genetic loci associated with response to abiotic stress
  • Genetic loci associated with response to abiotic stress
  • Genetic loci associated with response to abiotic stress

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0247] association mapping analysis

[0248] One hundred and ten elite non-rigid stalk (NSS) lines were grown in the high soil salinity location of location 1, and seedling vigor, also called early growth (EGRWTH), was scored on a per row basis. Lines were genotyped at 56,000 SNP markers across the maize genome. Genome-wide association mapping was then performed to identify markers associated with the early growth (EGRWTH) phenotype in position 1. Phenotype and genotype scores for all individuals tested were input into individual association analyses.

[0249] The most prominent peak of the early growth phenotype was identified in the region of 142.6-156.0 cM of chromosome 1 on a single meiosis-based genetic map. One haplotype within this region (referred to herein as haplotype "A") was associated with lower early growth, with an average score of 3.8 on a scale of 1 to 9, with 9 being the best. The average score for all other haplotypes was between 6 and 6.6. Haplotype "...

Embodiment 2

[0257] QTL mapping using double haploid breeding populations

[0258] A biparental mapping population was generated by crossing two elite non-rigid stalk inbreds (referred to herein as inbred A and inbred B) and comparing the salt tolerance phenotype. F from the hybrid 1 Individuals produced 179 double haploid lines. The double haploid population was grown at position 1 in a field with high levels of soil salinity. Growth and flowering trait data were collected for the populations, including seedling vigor also known as early growth (EGRWTH), pollen release days (SHD), silking days (SLK), plant height (PLTHT) and ear height (EARHT). The EGRWTH trait is a visual score of early seedling health on a scale of 1 to 9, with 9 being optimal. QTL mapping was performed using composite interval mapping in WinQTL Cartographer and strong QTLs were observed on chromosome 1 from 145.1 cM to 169.6 cM (on a gene map based on a single meiosis obtained internally) for all five agronomic tr...

Embodiment 3

[0265] Early growth phenotypes shaped by differential salinity tolerance

[0266] A hydroculture study was performed with inbreds selected from the test panel used in Example 1 to validate a surrogate assay to be used for mapping gene clones for chromosome 1 QTL in a controlled environment. Nine inbreds with different responses to soil salinity at position 1 were selected for testing under high-salinity hydroponic conditions. Ten plants of each inbred were grown for four weeks under normal and 100 mM NaCl conditions. After four weeks, the plants were scored on a scale of 1 to 9, with 9 being the healthiest and largest plant and 1 being the smallest or dead plant. Ten plants of each inbred were averaged to give a single score (Table 5). There was a strong correlation between inbreds responding to the high soil salinity condition at position 1 and inbreds responding to the 100 mM salt condition in the hydroculture study. Inbreds with haplotype A showed considerably less see...

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Abstract

Compositions and methods useful in identifying and counter-selecting maize plants with having enhanced yield-related traits relative to control plants under abiotic stress conditions such as salt stress and / or drought are provided herein. The methods use molecular genetic markers to identify, select and / or construct salt stress tolerant and / or drought tolerant maize plants. Also provided are methods to enhance tolerance to salt stress and / or drought in crop plants by transforming crop plants with the Zea mays antiporter / sodiunn ion transporter or by introducing favorable allelic variants of the Zea mays antiporter / sodiunn ion transporter gene via gene editing.

Description

[0001] Cross references to related patent applications [0002] This application claims the benefit of US Provisional Application 61 / 909,565, filed November 27, 2013, and US Provisional Application 61 / 950,345, filed March 10, 2014, the entire contents of which are incorporated herein by reference. [0003] References to Sequence Listings Submitted Electronically [0004] Electronically submit the official text of the sequence listing as a sequence listing in ASCII format via EFS-Web. submitted at the same time as this manual. The Sequence Listing contained in this file in ASCII format is part of the specification and is incorporated herein by reference in its entirety. technical field [0005] The field relates to plant breeding and methods of producing maize plants expressing enhanced yield-related traits under abiotic stress conditions, such as salt stress or drought. Background technique [0006] Abiotic stressors such as drought, salinity, cold, flooding and chemic...

Claims

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Application Information

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IPC IPC(8): A01H5/10
CPCA01H5/10C07K14/415C12N15/8273A01H6/4684
Inventor J.S.贾奎斯李柏林Z.刘
Owner EI DU PONT DE NEMOURS & CO
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