Plant strong salt-resistant gene AtNHXS1 and its coding protein and application

Abstract

The invention provides a novel plant strong salt tolerant gene AtNHXS1 acquired through DNA reshuffling technology, and the ionic transport activity of Na+ / H+ antiporter protein coded by the gene is stronger than that of wild Na+ / H+ antiporter protein ATNHX1. A nucleotide sequence of the gene as shown in SEQ ID NO:1 also comprises genes of the nucleotide sequence of sequence 1 in a sequence table with the homology between 70 and 100 percent, or a nucleotide sequence of an amino acid sequence of sequence 2 in a coded sequence table. The new Na+ / H+ antiporter has a protein of the amino acid sequence of sequence 2 in the sequence table, protein of the amino acid sequence of the sequence 2 with the homology between 70 and 100 percent, or protein which replaces, deletes or adds one or a plurality of amino acids in the amino acid sequence of the sequence 2 with the same homology. The invention also provides the construction of recombinant vector, the transgenic plant and other methods so as to apply the gene and the protein, thereby cultivating a novel variety of transgenic plant with strong capacity of salt tolerance or other improved biologic characters.

Description

technical field

[0001] The invention relates to the field of plant genetic engineering, in particular to the use of DNA shuffling technology to obtain significantly improved plant Na + / H + The antiporter gene, the invention also relates to the use of the gene to breed transgenic salt-tolerant plants. Background technique

[0002] Soil salinization is an important abiotic stress factor affecting crop production and ecological environment. At present, about 20% of the cultivated land and nearly 50% of the irrigated land in the world are seriously harmed by salinity (Flowler T J, Yeo A R. Breeding for salinity resistance in crop plants. Where next? Aust.J.Plant Physiol.1995 , 22: 875-884). There are about 500 million mu of saline-alkali land in my country, and its area has a tendency to increase continuously. Cultivating salt-tolerant plants is an effective way to promote the development and utilization of saline-alkali land, improve soil and ecological management. Theref...

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