5 results about "DNA shuffling" patented technology

The invention discloses a DNA shuffling method based on flanking sequence segmented amplification and application of the DNA shuffling method in screening herbicide-resistant hybrid enzymes, on the basis of a single-stranded DNA shuffling technology, the success rate of single-stranded preparation is increased by introducing flanking sequences and segmented amplification recombinant products into single-stranded amplification primers, and the yield of herbicide-resistant hybrid enzymes is increased. Meanwhile, the proportion of nonsense mutation in the mutation library is reduced. The method is expected to provide a new method for establishing a protein qualitative evolution mutation library. The method is used for shuffling the rice and corn hppd genes and screening to obtain the hybrid enzyme of which the resistance is stronger than that of parent rice and corn HPPD under the treatment of 300 mu M mesotrione.

This invention relates to the field of biomedicine and specifically to a porcine interferon-like recombinant protein and its applications. By constructing and screening a PoIFN-α DNA shuffling bacterial library, a highly active recombinant protein Porferon was obtained. Porferon exhibits potent anti-infective activity against a broad range of porcine pathogens, including viruses, bacteria, and parasites. Its biological activity is significantly superior to that of natural porcine interferon. As such, Porferon can be used in the development of therapeutics for the prevention or treatment of infectious diseases in pigs, offering a novel and effective solution for clinical applications in veterinary medicine.

The application provides a SARS-related coronavirus universal antigen and a preparation method and application thereof, and belongs to the technical field of immunity. The candidate antigen prepared by the application has higher S309 antibody and detection serum binding activity, the titer of serum obtained by immunizing mice with the candidate antigen has higher cross-binding activity to VOCs than that of template antigen immunized serum; meanwhile, the candidate antigen immunized serum has the ability to block the combination of VOCs and ACE2. Therefore, the candidate antigen prepared by the application can become a potential universal antigen or combination for inducing cross-neutralization activity for preventing SARS-related coronavirus infection. The strategy of preparing the candidate antigen with cross-neutralization immunogenicity based on DNA Shuffling and membrane mutation antigen screening technology is expected to be applied to universal vaccine research and development. The candidate universal antigen prepared by the application has the potential to be developed into a SARS-related coronavirus universal vaccine.

The invention belongs to the technical field of biology, and discloses an artificially modified cephalosporin C acylase mutant and application thereof. The mutant is obtained by carrying out molecular evolution on wild-type enzyme through a DNA shuffling technology, the amino acid sequence of the mutant is shown as SEQ ID NO: 1, and compared with the wild-type enzyme, the mutant has remarkably improved catalytic activity and can efficiently catalyze cephalosporin C deamidation to generate 7-aminocephalosporanic acid. The invention also discloses a nucleic acid molecule for coding the acylase, a recombinant vector and a recombinant host cell containing the nucleic acid molecule, and application of the acylase or the recombinant host in production of 7-ACA by using cephalosporin C as a substrate.

This invention discloses a high-performance laccase mutant constructed based on multi-source gene recombination and its applications. Using BspLac from Bacillus sp. SL-1 and BLTCLac from Bacillus licheniformis TCCC111219 as templates, a mutant library DS was constructed using DNA shuffling technology. Two high-performance laccase mutants, DS2 and DS5, were obtained through screening. Dynamic enzyme production analysis showed that the laccase activity of DS2 peaked at 1922 U / L after 24 hours, equivalent to twice that of the original strain; the laccase activity of DS5 peaked at 1337 U / L after 24 hours, equivalent to 1.4 times that of the original strain. Simultaneously, experimental verification showed that DS2 and DS5 achieved degradation rates of 83% and 89% for malachite green, respectively. This laccase mutant significantly improved the detoxification efficiency of synthetic dyes and possesses good potential for industrial application.