31results about How to "Eliminate Feedback Inhibition" patented technology

The present invention discloses a method for producing alcohol from mixed microbial pool degradable and fermented lignocellulose substance, relating to a method for producing alcohol by degradation of fermented lignocellulose substance, and solves the problems of high cost and long production cycle in production of alcohol by using the lignocellulose substance in the prior art. The method comprises the following steps: 1) crushing lignocellulose substance, soaking with H2SO4, and bursting by steam; 2) solid-liquid separating, adding nutritive liquid into solid-phase substance, and sterilizing; 3) adding distiller's yeast and cellulose degrading microbe into saccharifying fermentative substrate; 4) degradation saccharifying-fermenting synchronously, distilling and rectifying to obtain final product. The method for producing alcohol from mixed microbial pool degradable and fermented lignocellulose substance provided by the present invention has the advantages that: degradation and saccharification-fermentation are synchronously occurred, thus the degradation and saccharification-fermentation period is shortened by 40% while the productive technology is simplified and the use ratio of equipment is improved. Moreover, the feedback inhibition function to cellulose hydrolysis of monose is eliminated and both the raw material utilization rate and alcohol yield are improved.

The invention discloses a liquid-phase hydrogenation desulfurization reaction-separation coupled refining method for crude oil products in petroleum refining, which comprises the following steps: (1) mixing the crude oil products and hydrogen into a hydrogenation reactor, and refining them in hydrodesulfurization The hydrodesulfurization reaction is carried out under the action of the catalyst; the dosage of the hydrodesulfurization refining catalyst is 1.0%-10% of the crude oil quality, the hydrodesulfurization refining catalyst is a metal-supported catalyst, and the carrier is heavy oil catalytic cracking waste catalyst; (2) The gas-liquid separation of the above reaction products is carried out, and the gas phase stream is continuously extracted, washed and neutralized with lye; the liquid phase stream is extracted, which is a refined oil product. The invention can process crude oil refined from high-sulfur and high-nitrogen petroleum, and carry out hydrogenation desulfurization reaction-separation coupled refining of crude oil under relatively mild temperature and pressure operating conditions, greatly reducing equipment investment and operating costs.

The invention relates to a method for preparing rosin ester by coupling catalytic esterification and separation, which comprises the following operation steps: (1) mixing an alcohol compound, a rosin and a solvent, and dissolving; (2) putting the dissolved substance in the step (1) into an esterification reactor, and introducing 0.01-10.0-MPa CO2 to perform esterification reaction at 150-300 DEG C; (3) recovering the gas phase in the esterification reaction process, sending the gas phase into a gas-liquid separator through an expander, and returning the separated CO2 and newly supplemented CO2 into the esterification reactor through a turbine compressor; and (4) after reacting in the esterification reactor for 1.0-8.0 hours, relieving the pressure, and taking the obtained product out of the esterification reactor. The invention aims to provide a preparation method which has the advantages of lower cost and high rosin ester quality.

The present invention has the technology scheme of adopting microbial zymogene preparation containing neurospora and saccharomycete. The fermentation process with the microbial zymogene preparation of the present invention to produce feed or alcohol is to add the microbial zymogene preparation components simultaneously or gradually and to process the obtained material for producing feed or alcohol. The present invention has the beneficial effect that the coupled and synergistic fermentation with the microbial zymogene preparation can eliminate the feedback suppression and saccharide or ethanol product suppression in the fermentation, raise the protein content, soften material, generate spirit fragrance, improve the taste of feed and raise production efficiency and product quality.

The invention discloses a method for producing cellulase by mixed fermentation of aspergillus oryzae and fusarium chlamydosporum. The method comprises the following steps: inoculating the fusarium chlamydosporum and the aspergillus oryzae on a bagasse solid culture medium and performing mixed culture fermentation, wherein the culture temperature is 20-50 DEG C, and pH value is 4.0-10.0; finishing fermentation culture after the maximum cellulose yield is reached. The method disclosed by the invention has the benefits that through the mixed culture of the aspergillus oryzae and the fusarium chlamydosporum, high-transglycoside activity belta-glucosidase produced by the aspergillus oryzae can synthesize gentiobiose, and the gentiobiose can stimulate the synthesis of the cellulase from the fusarium chlamydosporum; through the mixed culture of the aspergillus oryzae and the fusarium chlamydosporum, the composition of a cellulase system is optimized, so that the overall enzyme activity of the cellulase system can be significantly improved.

The invention discloses a method for instantaneous vaporization and expansion inside grass slices-infiltration pulping. Soak the grass slices with an organic solvent for 5 to 120 minutes, then add boiling water at 100°C or water vapor whose temperature is higher than the boiling point of the organic solvent according to the mass ratio of the grass slices to water 1:1 to 1:20, and then under the condition of supercritical CO2 Under cooking pulping. The method of the invention causes the cell membrane to expand and rupture through the instant vaporization of the organic solvent between the fiber cells of the grass slice, so that the rate of removing intercellular lignin by cooking the grass slice is accelerated by 1-5 times, and the consumption of the organic solvent is reduced by 2-10 times.

The invention provides a method for extracting fumaric acid in fermentation broth in a continuous counter current manner by means of amine extractants. The method includes that filtered fermentation broth flow is added into an extracting tower to perform liquid-liquid extraction in the continuous counter current manner, the fermentation broth is used as heavy phase, the extraction system is used as light phase, extracts left the extraction tower is subjected to reextraction to generate fumaric acid or fumaric acid salt by reextraction, and the extraction system after reextraction returns the extraction tower and is used circularly; meanwhile, the extract can successively pass a primary organic solvent collection tank and a secondary organic solvent collection tank for secondary treatment, and finally the extracts flowing out returns the fermentation for reusing. The invention further provides a device for extracting fumaric acid in fermentation broth in a continuous counter current manner by means of amine extractants and application of a fermentation technology combined with the continuous counter-current extraction technology in continuous preparation of the fumaric acid. By the method, suppression to products in the process of fermentation of the fumaric acid can be effectively eliminated, and meanwhile, toxicity of the extracts to rhizopus oryzae can be avoided, and fermentation production level of the fumaric acid can be improved.

Aiming at the current situation that crop straws are not highly utilized as feed and the pollution of alcohol waste liquid is serious, the present invention discloses a method for preparing feed protein by using alcohol waste liquid and crop straws, so as to meet the comprehensive utilization of straws, animal husbandry feed protein demand, alcohol Objective requirements for harmless treatment and utilization of waste liquid. The invention adopts the process of "ammonification + compound enzymolysis + multi-strain three-step solid-state fermentation" to obtain a feed protein product with high degradation degree of cellulose and xylan and high content of feed protein. The product is rich in probiotics, vitamins, Nutrients such as enzymes, amino acids, short peptides, and high-quality protein have the advantages of promoting growth and development, improving feed utilization, enhancing immunity, improving intestinal microecology, and preventing diseases; the invention realizes high resource utilization of crop straw Value utilization and harmless treatment and resource utilization of alcohol waste liquid have broad application prospects.