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Method for extracting xylooligosaccharide from wheat bran

A technology of xylooligosaccharides and wheat bran, which is applied in the direction of fermentation, etc., can solve the problems of prolonging the processing chain of agricultural and sideline products, low xylanase activity, and low yield of xylan, achieving small investment, improving purity, The effect of simple production process

Inactive Publication Date: 2015-09-09
QINGDAO JIARUI BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the rich nutrition of wheat bran, the rich xylan is a good raw material for the preparation of xylo-oligosaccharides, but currently the preparation of xylo-oligosaccharides from bran mainly includes NaOH extraction, acid dry steaming and alkaline extraction. Combined method, KOH leaching method, H 2 o 2 Combined with lye extraction method, biological enzymatic hydrolysis method, etc., but there are disadvantages such as complex process, serious environmental pollution, low xylan yield, low xylanase activity, so the process of preparing xylooligosaccharides by traditional enzymatic method is simplified , to provide a new way for the preparation of xylo-oligosaccharides, and to comprehensively utilize wheat bran, a by-product of agricultural crops, to extend the processing chain of agricultural by-products, and to turn waste bran into treasure, which has very important social significance and economic value

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for extracting xylo-oligosaccharides from wheat bran, the specific preparation steps are as follows:

[0023] (1) Pulverization: select bran without mildew and pulverize it through a 40-mesh sieve;

[0024] (2) Cooking: Cook at 150°C for 0.5h, and adjust the mass ratio of bran to water to 1:10 after cooling;

[0025] (3) Ultrasonic-assisted enzymatic hydrolysis to remove starch: adjust the pH of the wheat bran suspension to 5.5-7.5, add α-amylase at a ratio of 3% of the mass of wheat bran, and perform enzymatic hydrolysis under the action of ultrasonic waves. The temperature is 70-80°C, and the ultrasonic power is 300W. During this period, a stirrer is used to stir at a constant speed, and the iodine solution is used to check until the starch is completely hydrolyzed; after the enzymolysis is completed, the filtrate is starch hydrolyzate, and 5 times the mass ratio of distilled water to obtain a suspension, for subsequent use;

[0026] (4) Ultrasonic-assisted...

Embodiment 2

[0036] A method for extracting xylo-oligosaccharides from wheat bran, the specific preparation steps are as follows:

[0037] (1) Pulverization: select bran without mildew and pulverize it through a 40-mesh sieve;

[0038] (2) Cooking: Cook at 150°C for 0.8h, and adjust the mass ratio of bran to water to 1:9 after cooling;

[0039] (3) Ultrasonic-assisted enzymatic hydrolysis to remove starch: adjust the pH of the wheat bran suspension to 5.5-7.5, add α-amylase at a ratio of 4% by mass of wheat bran, and perform enzymatic hydrolysis under the action of ultrasonic waves. The temperature is 70-80°C and the ultrasonic power is 300W. During this period, a stirrer is used to stir at a constant speed, and the iodine solution is used to check until the starch is completely hydrolyzed; after the enzymolysis is completed, filter, and the filtrate is a starch hydrolyzate. Add 4 times the mass ratio of distilled water to obtain a suspension, for subsequent use;

[0040] (4) Ultrasonic-...

Embodiment 3

[0050] A method for extracting xylo-oligosaccharides from wheat bran, the specific preparation steps are as follows:

[0051] (1) Pulverization: select bran without mildew and pulverize it through a 40-mesh sieve;

[0052] (2) Cooking: Cook at 150°C for 1 hour, and adjust the mass ratio of bran to water to 1:8 after cooling;

[0053] (3) Ultrasonic-assisted enzymatic hydrolysis to remove starch: adjust the pH of the wheat bran suspension to 5.5-7.5, add α-amylase at a ratio of 5% of the mass of wheat bran, and perform enzymatic hydrolysis under the action of ultrasonic waves. The temperature is 70-80°C, and the ultrasonic power is 300W. During this period, a stirrer is used to stir at a constant speed, and the iodine solution is used to check until the starch is completely hydrolyzed; after the enzymolysis is completed, filter, and the filtrate is starch hydrolyzate. Add 3 times the mass ratio of distilled water to obtain a suspension, for subsequent use;

[0054] (4) Ultras...

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Abstract

The invention discloses a method for extracting xylooligosaccharide from wheat bran. The method is used for preparing xylooligosaccharide with efficacies of promoting bifidobacterium reproduction, preventing constipation, reducing cholesterol, protecting the liver and the like by using byproduct of agricultural and sideline product processing, i.e., bran as a raw material and by using microbial fermentation and biological enzymolysis technologies, the quality of the obtained xylooligosaccharide product satisfies the industrial standard requirement of xylooligosaccharide, the high-efficiency utilization of the bran is realized, the use value of the bran is improved and the popularization prospect is better. The method for extracting xylooligosaccharide from wheat bran has the advantages that firstly the yield of xylooligosaccharide is improved through a combined preparation process of compound microbial solid fermentation and ultrasonic assisted enzymolysis; secondly the purity of the xylooligosaccharide product is obviously improved through the ultrasonic assisted enzymolysis for removing protein and starch and enzymolysis of phytase and mannase; and thirdly the capability of xylooligosaccharide fermentation and enzymolysis is improved through multi-bacteria mixed fermentation; fourthly the production process is simple, the energy consumption is low, no environmental pollution is caused, the investment is small and the mass production is facilitated.

Description

technical field [0001] The invention relates to a method for extracting xylooligosaccharides from wheat bran, and belongs to the technical field of comprehensive utilization of grain processing by-products. Background technique [0002] With the development of society and the continuous improvement of people's material living standards, people's attention to health has also been greatly improved, and the appearance of functional foods has set off a new upsurge in research and development. Among them, functional oligosaccharides, as a functional food base material, have the functions of promoting the proliferation of bifidobacteria, regulating intestinal flora, inhibiting endotoxin, protecting liver function, regulating gastrointestinal function, preventing constipation and diarrhea, activating immunity, Its unique physiological functions such as anti-aging, anti-tumor, lowering serum cholesterol, lowering blood pressure, low energy or no energy, and not causing dental carie...

Claims

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Application Information

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IPC IPC(8): C12P19/14
Inventor 侯文燕董静静赵建伟
Owner QINGDAO JIARUI BIOLOGICAL TECH
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