91 results about "Zymase" patented technology

The invention relates to the technical field of meat product fermentation and enzymolysis, and provides a meat product fermentation and enzymolysis device and an enzymolysis method thereof.The meat product fermentation and enzymolysis device comprises an outer cylinder, a core cylinder, a circulating stirring paddle and a fermentation and enzymolysis control unit; the top of the cylinder body of the outer cylinder is provided with a feeding port, and the bottom is provided with a conical cavity; the output port is connected to the bottom of the conical cavity; the core cylinder is arranged in the middle of the outer cylinder and divides the interior of the outer cylinder into a center cavity and an annular cavity, and the top and the bottom of the center cavity are communicated with the top and the bottom of the annular cavity respectively. The circulating stirring paddle is arranged in the central cavity; the fermentation and enzymolysis control unit controls the circulation stirring paddle to rotate, stirs and drives meat to move upwards from the central cavity to enter the annular cavity, moves downwards along the annular cavity and then returns to the central cavity, and the meat and ingredients are mixed in the circulation process; according to the invention, the mixing uniformity between the meat and between the meat and the ingredients containing the enzyme preparation in the fermentation and enzymolysis process is realized, and the taste quality such as the uniformity and toughness of the enzymolysis meat is improved.

The invention discloses coprinus cinereus sourced laccase as well as a recombinant expression strain and a high-efficiency expression method thereof, and belongs to the technical field of microbiology and bioengineering. According to the invention, aspergillus niger MA70.15 is used as an experimental strain, and a random integration strategy is adopted, so that a pC3-PcitA-SPGlaA-lcc9-TtrpC-pyrG expression vector is integrated in an upstream region of a promoter of host bacteria citrate synthase in a form of nine continuous copies. In order to explore the influence of particles and osmotic regulators in a fermentation culture medium on laccase production through fermentation of recombinant aspergillus niger, the particles and osmotic regulators of different types and concentrations are added, so that the enzyme activity of the laccase fermented in a shake flask of the recombinant strain AnRcgL1 reaches 1566.7 U / L, the enzyme activity fermented in a 1L fermentation tank reaches 1961 U / L, and the recombinant high-efficiency expression of the laccase Lcc9 in the aspergillus niger is successfully realized.

The invention discloses a preparation method and application of a three-thorn fruit fermentation broth capable of regulating cell rhythm, and the preparation method comprises the following steps: (1) adding nitraria fruit, yellow thorn fruit and prinsepia utilis royle into a fermentation enzymatic hydrolysate, and carrying out enzymolysis to prepare a fermentation substrate; and (2) inoculating schizophyllum commune into the fermentation substrate for fermentation, and sterilizing to obtain the schizophyllum commune fermentation substrate, wherein a preparation method of the fermented enzymatic hydrolysate comprises the following steps: adding water into a substrate, sterilizing to obtain a culture medium, inoculating, performing aerobic fermentation, and centrifuging, thereby obtaining the fermented enzymatic hydrolysate. The fermentation enzymatic hydrolysate comprises agaricus blazei fermentation enzymatic hydrolysate or saccharomyces cerevisiae fermentation enzymatic hydrolysate. According to the method, saccharomyces cerevisiae or agaricus blazei fermentation enzymatic hydrolysate is firstly used for enzymolysis extraction, wall breaking is conducted on plants preliminarily, active ingredients in the plants are released, then schizophyllum commune is further fermented, further release and conversion are achieved, ideal effects of oxidation resistance, moisturizing, soothing, tightening, whitening and the like are achieved by adjusting cell rhythm, fermentation conditions are mild, and the method is suitable for industrial production. Good application prospects are realized.

The invention belongs to the technical field of feed production, and discloses low-viscosity fermented enzymolysis soybean meal and a preparation method thereof. The preparation method comprises the following steps: adding extruded soybeans into soybean meal as a hydrolysis raw material, wherein the mass ratio of the extruded soybeans in the hydrolysis raw material is 10-30%; mixing the hydrolysis raw material with hot water according to a mass ratio of 1: (0.25-0.35), adding lipase accounting for 1-3% of the mass of the hydrolysis raw material, and hydrolyzing at 35-45 DEG C for 12-36 hours to obtain a hydrolysis material; shearing and emulsifying the hydrolyzed material, adjusting the water content of the hydrolyzed material to 35-50%, inoculating a bacterium enzyme compound, and performing fermentation and enzymolysis; the lipase is lipase A or a compound formed by combining the lipase A and other lipase according to the mass ratio of 1: 1. The flowability and sensory viscosity of the obtained fermented enzymolysis soybean meal are obviously improved, the production and application efficiency is improved by 60%, the fermented enzymolysis soybean meal has fermented acid fragrance and also generates animal-friendly meat flavor, and after the fermented enzymolysis soybean meal is applied to feed, the diarrhea rate of suckling pigs is reduced by 65%, the feed intake is increased by 20%, and the feed conversion ratio is reduced by 8%.

The invention discloses streptomyces mobaraensis for high yield of heat-resistant glutamine transaminase, and belongs to the technical field of gene engineering. According to the invention, Pgapdh and PsmY2022-2 are combined to obtain the tandem promoters PsmY2022-2-Pgapdh and Pgapdh-PsmY2022-2, and the constructed tandem promoters are used for expression of regulation and control, so that the shake flask fermentation enzyme activity of the constructed recombinant bacteria reaches 64.94 U / mL, the expression quantity of heat-resistant glutamine transaminase is improved, and a theoretical foundation is laid for efficient expression of other genes in streptomyces mobaraensis.

The invention relates to the technical field of genetic engineering, and discloses a genetically engineered bacterium for producing beta-ionone at high yield and application of the genetically engineered bacterium. A synthetic biological plasmid modularization technology is adopted, coding genes of carotenoid cleavage dioxygenase NtCCD1 in tobacco are excavated, and specific soluble protein tags including a thioredoxin tag, a maltose binding protein tag and a glutathione transferase tag are screened, so that the content of the carotenoid cleavage dioxygenase NtCCD1 in the tobacco is determined. The NtCCD1 is subjected to single-point mutation modification of mutation of 431st-site histidine into tyrosine, lysine or glutamine, and the fermentation enzyme and the gene engineering bacterium which can efficiently catalyze splitting decomposition of beta-carotene in tobacco to produce beta-ionone are constructed. The biosynthesis of the tobacco aroma component beta-ionone is realized, and the method has high efficiency.

The invention relates to a fermentation enzymolysis technology, in particular to a production method for optimizing natural cheese essence by utilizing an electric field auxiliary technology. The method comprises the following steps: preheating milk, adjusting the pH value, adding trypsin and lipase, and uniformly mixing; and putting the obtained milk containing the enzyme into an electric field assisted reactor, carrying out electric field assisted fermentation enzymolysis under a stirring condition, and carrying out post-treatment on the obtained reaction product to obtain the cheese essence. By combining the electric field technology and the enzymolysis process, the reaction efficiency and the product quality are remarkably improved.

This invention belongs to the field of traditional Chinese medicine fermentation technology, specifically involving a high-enzyme-active Liu Shen Qu (a type of fermented medicinal herb) and its fermentation process. The process first processes red beans, bitter almonds, artemisia annua, cocklebur, and polygonum hydropiper into a medicinal porridge, which is then mixed with wheat flour and a specific fermenting agent to form a soft mass. Its innovation lies in the use of a Bacillus subtilis trehalose protective agent complex and Aspergillus oryzae chitosan microspheres. These two fermenting agents are prepared by spray drying encapsulation and ionogel methods, respectively, significantly improving the survival rate and stability of the fermenting microorganisms during processing and storage. The soft mass undergoes two stages of precisely controlled temperature and humidity fermentation, and finally, after shaping and segmented drying, the finished product is obtained. This process effectively solves the problems of low enzyme activity and poor stability in traditional Liu Shen Qu fermentation, resulting in a Liu Shen Qu product with high digestive enzyme activity and stable, uniform quality.

The invention belongs to the field of biological agriculture fermentation, and particularly relates to snakegourd fruit nutrition powder and a preparation method and application thereof.The snakegourd fruit nutrition powder is prepared by conducting primary subzero treatment on snakegourd fruit roots in liquid carbon dioxide, soaking the snakegourd fruit roots subjected to primary subzero treatment in egg yolk hydrolysate for treatment, soaking the snakegourd fruit roots subjected to hydrolysis egg yolk soaking in liquid nitrogen for secondary subzero treatment, and conducting secondary subzero treatment. Soaking the secondarily cryogenic trichosanthes kirilowii maxim roots in liquid carbon dioxide for three-time cryogenic treatment, and then inoculating a fermentation strain for fermentation, enzymolysis and drying. According to the fermentation method, the fermentation uniformity is good, the trichosanthes kirilowii maxim roots do not need to be crushed, and the problem that the trichosanthes kirilowii maxim roots are difficult to ferment due to the fact that raw materials are compact in structure, microbial inoculation and growth are limited, moisture and ventilation are uneven and the like can be solved by conducting subzero treatment on the trichosanthes kirilowii maxim roots three times in cooperation with egg yolk hydrolysate dipping treatment. The fermented feed liquid obtained by fermenting the trichosanthes kirilowii roots can be prepared into trichosanthes kirilowii nutrition powder, and the fermentation residues can be used as a pig feed additive.

The invention relates to a rosa roxburghii tratt fruit fermentation enzyme unfrozen dry product and a preparation method and application thereof, and relates to the technical field of rosa roxburghii tratt fruit processing.The preparation method comprises the following steps that fresh rosa roxburghii tratt fruits are squeezed and filtered, and rosa roxburghii tratt fruit juice is obtained; performing high-pressure disinfection on the roxburgh rose juice, then adding water, and performing stirring and heating to obtain a roxburgh rose stock solution; adding a fermentation strain substance into the rosa roxburghii tratt stock solution for fermentation treatment to obtain a rosa roxburghii tratt stock solution enzyme solution; and performing enzymolysis treatment on the roxburgh rose juice enzyme liquid to obtain roxburgh rose fermented enzymatic hydrolysate, and the like. Through the innovative enzymolysis fermentation freeze-drying technology, the stability and bioavailability of active substances in the roxburgh rose are improved, a series of problems in the traditional processing process are solved, the efficacy of the roxburgh rose is remarkably improved, and the application range is wider. A powerful technical support is provided for deep development of the rosa roxburghii tratt industry and innovation in the field of homology of medicine and food.

The invention relates to the technical field of health food, in particular to a method for preparing honeycomb spleen cream honey. According to the method for preparing the honeycomb spleen cream honey, honeycomb cells on honeycomb spleen are pressurized and boiled, nutrient substances in the honeycomb cells are dissolved in water, a honeycomb extracting solution is obtained, then the honeycomb extracting solution is concentrated to obtain honeycomb spleen cream, then honey is added, heating treatment is conducted, and standing is conducted to obtain the honeycomb spleen cream honey. On one hand, the honeycomb spleen paste can be stored for a long time and generally has a shelf life of 5-10 years, and on the other hand, the heating treatment process after the honey is added can remove excessive moisture in the honey and inactivate fermentation enzyme in the honey, so that the honeycomb spleen paste honey product can be stored for a long time more favorably.

The invention provides a compound feed additive produced by liquid submerged fermentation and a preparation method thereof, the compound feed additive comprises xylanase and acid protease, and the compound feed additive is obtained by co-fermentation of aspergillus niger and kluyveromyces marxianus with soybean meal, defatted rice bran and inorganic salt as fermentation substrates. Kluyveromyces marxianus can eliminate xylose, amino acid and other feedback inhibition factors generated in the aspergillus niger fermentation process, aspergillus niger fermentation is promoted to generate xylanase and acid protease, and the fermentation enzyme activity is improved. After xylanase and acid protease generated in the growth and metabolism process of the aspergillus niger, bioactive substances such as alpha-galactosidase, pectinase and beta-glucanase which are generated along with the aspergillus niger and kluyveromyces marxianus are added into feed as additives at the same time, the digestion and absorption of livestock and poultry on nutrient substances and the health of animals can be promoted; and the application advantages of the product are improved.

The present invention relates to a method for the production of protein glutaminase (protein-glutaminase; the EC is 3.5. 1.44; the present invention relates to a seed medium composition and a fermentation medium composition for a PG enzyme (e.g., PG enzyme). The invention relates to a method for producing PG enzyme through fermentation. The method comprises the following steps: fermenting for 3-8 hours by adopting a fermentation medium composition, and feeding a nutrient composition, and supplementing the surfactant composition when the fermentation is carried out for 13-18 hours. According to the method, the fermentation period can be shortened by 29%, the fermentation enzyme activity is improved, and the enzyme activity can reach 12.3 U / ml after 20-hour fermentation.

The invention relates to the technical field of puree food, in particular to infant yoghurt puree and a preparation method thereof. The fruit paste is prepared by the method. The method comprises the following steps: weighing required amounts of raw material components including raw milk, a leavening agent, an enzyme preparation and thickening fruits; the leavening agent comprises a heterotrophic fermentation strain and a bifidobacterium fermentation strain; the enzyme preparation comprises esterifying enzyme and lactase; the method comprises the following steps: firstly, concentrating raw milk by adopting an RO membrane, and then carrying out degassing treatment, first homogenization treatment and first sterilization treatment on the raw milk; adding a leavening agent and an enzyme preparation into the raw milk subjected to the first sterilization treatment, and performing fermentation and enzymolysis treatment to obtain fermented milk; carrying out shearing and refining treatment on fermented milk, adding the pretreated thickened fruits, uniformly mixing, and carrying out second homogenization treatment to obtain a homogenized material liquid; and canning the homogenized material liquid and carrying out second sterilization treatment to obtain a fruit paste product. The fermentation time can be shortened, the taste and smell of the product are improved, and the shelf life stability of the product is improved.

The invention discloses the construction of a recombinant bacterium expressing chitinase and the preparation of a high-enzymatic-activity mutant, belonging to the technical fields of genetic engineering and enzyme engineering. In the invention, the endogenous strong promoter SpoVG and the signal peptide YqxI are used to realize the secretory expression of chitinase BlChiA derived from Bacillus licheniformis in Bacillus subtilis WS9, and further site-directed mutagenesis is carried out to improve the expression level and acid tolerance of chitinase. The fermentation enzyme activity of the obtained chitinase mutant E336K / A472T reaches 2.67 U / mL. At the same time, the optimal reaction pH of the chitinase mutant is 5.0, and it has good stability in the range of pH 3.0 - pH 6.0, and the residual enzyme activity reaches more than 90%. It has the advantages of good stability and strong acid tolerance, and is suitable for obtaining oligosaccharide products with high conversion rate in industrial production.

The invention provides a fermented feed for feeding nursery pigs as well as a preparation method and application of the fermented feed. The preparation method comprises the following steps: mixing a fermentation substrate, a fermentation additive, zymophyte and a fermentation enzyme to obtain a mixed material; performing anaerobic fermentation on the mixed material to obtain a fermented feed; wherein the fermentation substrate comprises corn DDGS, rice bran meal, bran and wheat, the fermentation additive comprises yeast cell walls and yeast powder, the zymophyte comprises saccharomycetes, lactic acid bacteria and bacillus, and the fermentation enzyme comprises protease and cellulase. The fermented feed provided by the invention can solve the problem of lack of corn and soybean meal-free feed suitable for nursery pigs in the prior art, and is suitable for the field of animal feed; the feed is applied to nursery pigs in a liquid feeding manner, can improve the growth performance, improve the intestinal health, enhance the immunity and increase the breeding benefits, and has a wide application prospect.