69 results about "Zymase" patented technology

The invention discloses a preparation method and application of a three-thorn fruit fermentation broth capable of regulating cell rhythm, and the preparation method comprises the following steps: (1) adding nitraria fruit, yellow thorn fruit and prinsepia utilis royle into a fermentation enzymatic hydrolysate, and carrying out enzymolysis to prepare a fermentation substrate; and (2) inoculating schizophyllum commune into the fermentation substrate for fermentation, and sterilizing to obtain the schizophyllum commune fermentation substrate, wherein a preparation method of the fermented enzymatic hydrolysate comprises the following steps: adding water into a substrate, sterilizing to obtain a culture medium, inoculating, performing aerobic fermentation, and centrifuging, thereby obtaining the fermented enzymatic hydrolysate. The fermentation enzymatic hydrolysate comprises agaricus blazei fermentation enzymatic hydrolysate or saccharomyces cerevisiae fermentation enzymatic hydrolysate. According to the method, saccharomyces cerevisiae or agaricus blazei fermentation enzymatic hydrolysate is firstly used for enzymolysis extraction, wall breaking is conducted on plants preliminarily, active ingredients in the plants are released, then schizophyllum commune is further fermented, further release and conversion are achieved, ideal effects of oxidation resistance, moisturizing, soothing, tightening, whitening and the like are achieved by adjusting cell rhythm, fermentation conditions are mild, and the method is suitable for industrial production. Good application prospects are realized.

The invention discloses streptomyces mobaraensis for high yield of heat-resistant glutamine transaminase, and belongs to the technical field of gene engineering. According to the invention, Pgapdh and PsmY2022-2 are combined to obtain the tandem promoters PsmY2022-2-Pgapdh and Pgapdh-PsmY2022-2, and the constructed tandem promoters are used for expression of regulation and control, so that the shake flask fermentation enzyme activity of the constructed recombinant bacteria reaches 64.94 U / mL, the expression quantity of heat-resistant glutamine transaminase is improved, and a theoretical foundation is laid for efficient expression of other genes in streptomyces mobaraensis.

The invention relates to the technical field of genetic engineering, and discloses a genetically engineered bacterium for producing beta-ionone at high yield and application of the genetically engineered bacterium. A synthetic biological plasmid modularization technology is adopted, coding genes of carotenoid cleavage dioxygenase NtCCD1 in tobacco are excavated, and specific soluble protein tags including a thioredoxin tag, a maltose binding protein tag and a glutathione transferase tag are screened, so that the content of the carotenoid cleavage dioxygenase NtCCD1 in the tobacco is determined. The NtCCD1 is subjected to single-point mutation modification of mutation of 431st-site histidine into tyrosine, lysine or glutamine, and the fermentation enzyme and the gene engineering bacterium which can efficiently catalyze splitting decomposition of beta-carotene in tobacco to produce beta-ionone are constructed. The biosynthesis of the tobacco aroma component beta-ionone is realized, and the method has high efficiency.

The invention relates to a fermentation enzymolysis technology, in particular to a production method for optimizing natural cheese essence by utilizing an electric field auxiliary technology. The method comprises the following steps: preheating milk, adjusting the pH value, adding trypsin and lipase, and uniformly mixing; and putting the obtained milk containing the enzyme into an electric field assisted reactor, carrying out electric field assisted fermentation enzymolysis under a stirring condition, and carrying out post-treatment on the obtained reaction product to obtain the cheese essence. By combining the electric field technology and the enzymolysis process, the reaction efficiency and the product quality are remarkably improved.

This invention belongs to the field of traditional Chinese medicine fermentation technology, specifically involving a high-enzyme-active Liu Shen Qu (a type of fermented medicinal herb) and its fermentation process. The process first processes red beans, bitter almonds, artemisia annua, cocklebur, and polygonum hydropiper into a medicinal porridge, which is then mixed with wheat flour and a specific fermenting agent to form a soft mass. Its innovation lies in the use of a Bacillus subtilis trehalose protective agent complex and Aspergillus oryzae chitosan microspheres. These two fermenting agents are prepared by spray drying encapsulation and ionogel methods, respectively, significantly improving the survival rate and stability of the fermenting microorganisms during processing and storage. The soft mass undergoes two stages of precisely controlled temperature and humidity fermentation, and finally, after shaping and segmented drying, the finished product is obtained. This process effectively solves the problems of low enzyme activity and poor stability in traditional Liu Shen Qu fermentation, resulting in a Liu Shen Qu product with high digestive enzyme activity and stable, uniform quality.

The invention belongs to the field of biological agriculture fermentation, and particularly relates to snakegourd fruit nutrition powder and a preparation method and application thereof.The snakegourd fruit nutrition powder is prepared by conducting primary subzero treatment on snakegourd fruit roots in liquid carbon dioxide, soaking the snakegourd fruit roots subjected to primary subzero treatment in egg yolk hydrolysate for treatment, soaking the snakegourd fruit roots subjected to hydrolysis egg yolk soaking in liquid nitrogen for secondary subzero treatment, and conducting secondary subzero treatment. Soaking the secondarily cryogenic trichosanthes kirilowii maxim roots in liquid carbon dioxide for three-time cryogenic treatment, and then inoculating a fermentation strain for fermentation, enzymolysis and drying. According to the fermentation method, the fermentation uniformity is good, the trichosanthes kirilowii maxim roots do not need to be crushed, and the problem that the trichosanthes kirilowii maxim roots are difficult to ferment due to the fact that raw materials are compact in structure, microbial inoculation and growth are limited, moisture and ventilation are uneven and the like can be solved by conducting subzero treatment on the trichosanthes kirilowii maxim roots three times in cooperation with egg yolk hydrolysate dipping treatment. The fermented feed liquid obtained by fermenting the trichosanthes kirilowii roots can be prepared into trichosanthes kirilowii nutrition powder, and the fermentation residues can be used as a pig feed additive.

The invention relates to a rosa roxburghii tratt fruit fermentation enzyme unfrozen dry product and a preparation method and application thereof, and relates to the technical field of rosa roxburghii tratt fruit processing.The preparation method comprises the following steps that fresh rosa roxburghii tratt fruits are squeezed and filtered, and rosa roxburghii tratt fruit juice is obtained; performing high-pressure disinfection on the roxburgh rose juice, then adding water, and performing stirring and heating to obtain a roxburgh rose stock solution; adding a fermentation strain substance into the rosa roxburghii tratt stock solution for fermentation treatment to obtain a rosa roxburghii tratt stock solution enzyme solution; and performing enzymolysis treatment on the roxburgh rose juice enzyme liquid to obtain roxburgh rose fermented enzymatic hydrolysate, and the like. Through the innovative enzymolysis fermentation freeze-drying technology, the stability and bioavailability of active substances in the roxburgh rose are improved, a series of problems in the traditional processing process are solved, the efficacy of the roxburgh rose is remarkably improved, and the application range is wider. A powerful technical support is provided for deep development of the rosa roxburghii tratt industry and innovation in the field of homology of medicine and food.

The invention relates to the technical field of puree food, in particular to infant yoghurt puree and a preparation method thereof. The fruit paste is prepared by the method. The method comprises the following steps: weighing required amounts of raw material components including raw milk, a leavening agent, an enzyme preparation and thickening fruits; the leavening agent comprises a heterotrophic fermentation strain and a bifidobacterium fermentation strain; the enzyme preparation comprises esterifying enzyme and lactase; the method comprises the following steps: firstly, concentrating raw milk by adopting an RO membrane, and then carrying out degassing treatment, first homogenization treatment and first sterilization treatment on the raw milk; adding a leavening agent and an enzyme preparation into the raw milk subjected to the first sterilization treatment, and performing fermentation and enzymolysis treatment to obtain fermented milk; carrying out shearing and refining treatment on fermented milk, adding the pretreated thickened fruits, uniformly mixing, and carrying out second homogenization treatment to obtain a homogenized material liquid; and canning the homogenized material liquid and carrying out second sterilization treatment to obtain a fruit paste product. The fermentation time can be shortened, the taste and smell of the product are improved, and the shelf life stability of the product is improved.

The invention discloses the construction of a recombinant bacterium expressing chitinase and the preparation of a high-enzymatic-activity mutant, belonging to the technical fields of genetic engineering and enzyme engineering. In the invention, the endogenous strong promoter SpoVG and the signal peptide YqxI are used to realize the secretory expression of chitinase BlChiA derived from Bacillus licheniformis in Bacillus subtilis WS9, and further site-directed mutagenesis is carried out to improve the expression level and acid tolerance of chitinase. The fermentation enzyme activity of the obtained chitinase mutant E336K / A472T reaches 2.67 U / mL. At the same time, the optimal reaction pH of the chitinase mutant is 5.0, and it has good stability in the range of pH 3.0 - pH 6.0, and the residual enzyme activity reaches more than 90%. It has the advantages of good stability and strong acid tolerance, and is suitable for obtaining oligosaccharide products with high conversion rate in industrial production.

The present invention belongs to the field of biotechnology, and specifically relates to a Pichia pastoris engineering bacterium with high yield of β-glucosidase. The amino acid sequence of the β-glucosidase mutant is shown in SEQ ID No. 4. By means of genetic engineering, a vector containing the mutant gene is introduced into a host to obtain a genetically engineered bacterium. Through verification of the fermentation performance, the fermentation enzyme activity of β-glucosidase can reach more than 51,000 U / mL. The optimal pH of the β-glucosidase expressed by the mutant is 4.0, which is 0.5 lower than that before mutation. The optimal temperature is 45 °C. After being treated at pH 3.5 for 24 h, the relative activity still remains about 80%. Compared with that before mutation, the acid resistance is improved, which is more conducive to application in the cellulose bioconversion industry.

The invention belongs to the technical field of dairy food preparation, and relates to a multi-layer milk skin and a preparation method thereof.The preparation method comprises the steps that firstly, fresh milk is subjected to ultrasonic-assisted fermentation and directional enzymolysis, and modified milk slurry rich in flavor precursor substances is prepared; then, a multi-layer structure is constructed in the mode of multi-round layered slurry laying and accurate temperature control superposition, and a gradient functional layer is formed; and finally, performing staged dynamic drying on the multi-layer blank body, and coating the multi-layer blank body with a multifunctional edible film coating liquid. According to the technology, a multi-layer structure with gradient is actively constructed, and the multi-layer taste of the product is achieved; meanwhile, through the cooperation of fermentation, enzymolysis and controlled Maillard reaction, the product is endowed with a compound flavor of interlaced delicate flavor, mellow flavor and scorch aroma; and the outer coating effectively prolongs the shelf life of the product. The technical defects of single structure, insufficient flavor, unstable texture and short shelf life of the traditional milk skin are overcome.

This invention discloses a yeast fermentation enzymatic hydrolysis device and method, relating to the field of yeast fermentation enzymatic hydrolysis technology. It includes a base and a fermentation tank, rotatably mounted on the top of the base; a hollow stirring shaft rotatably mounted inside the fermentation tank, with several blades mounted on its side; a shaft seal mounted on the stirring shaft for support and sealing the connection between the fermentation tank and the stirring shaft; a first drive unit located on the top of the fermentation tank for driving the stirring shaft to rotate; a second drive unit located on the top of the base for driving the fermentation tank to rotate; and a cleaning unit located inside the stirring shaft for cleaning the connection between the stirring shaft and the blades, as well as the gaps inside the shaft seal. This invention can clean the gaps at the connection between the stirring shaft and the shaft seal, as well as the substrate residue remaining on the lower end face of the connection between the stirring shaft and the blades, preventing substrate residue in the fermentation tank from causing mold growth and affecting the next fermentation.

The utility model discloses a kind of fermentation equipment for beer production belongs to fermentation equipment technical field, including jar main body, the side of jar main body is equipped with auxiliary assembly, including cooling assembly, the side of cooling assembly is connected with the outer edge of jar main body, cooling assembly can cool the outside of jar main body, cooling assembly includes cooling cover, this fermentation equipment for beer production, the cooling assembly of the utility model is used in cooperation with power component, in the process of stirring can realize internal solution and fermentation enzyme fast mix, simultaneously in the process of fermentation, jar body center part and the solution close to jar body inner wall can be realized replacement, so that the solution in jar body can all be evenly contacted to the temperature replacement process of jar body outer cooling water, to realize the process of uniform cooling, avoid the condition that jar body core liquid is not easy to cool due to the jacket of jar body shell direct cooling jar body, shorten cooling time, improve the cooling efficiency of equipment.

The present invention provides a puffing composition containing starch derived from legumes and grains, which promotes puffing and imparts the viscoelasticity (tension) characteristic of the starch network. [Solution] A puffing composition containing starch derived from legumes and / or grains, and satisfying all of the following conditions (1) to (7). (1) The starch content is 3% by mass or more and 100% by mass or less on a wet mass basis. (2) The dry weight moisture content is 0% by mass or more and less than 150% by mass. (3) The dietary fiber content is 3.0% by mass or more and less than 40% by mass on a wet mass basis. (4) The plant-derived polysaccharide content is 0.1% by mass or more and 100% by mass or less on a wet mass basis. (5) The ratio of [Value β] / [Value α] when measured by the following method a is between 0 and 100. [Value α]: Viscosity at breakdown (cP) during the heating stage (a1). [Value β]: Peak viscosity (cP) during the cooling stage (a2).

The invention belongs to the technical field of biology, and particularly relates to a method for preparing composite pentapeptide with a lipid-lowering and blood-glucose-lowering function by utilizing an enzymolysis-fermentation-enzymolysis technology and application of the composite pentapeptide. According to the composite pentapeptide with the lipid-lowering and blood-glucose-reducing functions, the soybeans, the highland barley, the folium mori, the ginseng and the tricholoma matsutake are specifically combined, synergistic interaction can be achieved, and the lipid-lowering and blood-glucose-reducing effects are remarkably improved. In addition, the invention also provides a scheme for preparing the polypeptide by using an enzymolysis-fermentation-enzymolysis triple biotransformation technology combined process, which is beneficial to decomposing plant macromolecular substances into easily absorbed micromolecular nutritional ingredients, and simultaneously directionally extracting the five-dimensional plant composite pentapeptide with the effect of reducing blood sugar and blood fat. The biological activity and functionality of the composite pentapeptide are improved, and high-quality raw materials are provided for prevention and treatment of diabetes and hyperlipidemia. Therefore, the technical scheme of the invention has the advantages of safety, reliability, good effect and no side effect.

The application relates to the technical field of automation production, and discloses an automatic production method for fish solubles preparation, which comprises the following steps: confirming an automatic production system comprising a data fitting unit, a parameter input unit and an automatic production unit; confirming to receive a data fitting instruction from the data fitting unit; confirming a fitting database based on the data fitting instruction; searching for a fermentation enzyme type set and a raw material fish type set in the fitting database; sending the fermentation enzyme type set and the raw material fish type set to an initiating end of an automatic production instruction; receiving a target fermentation enzyme type and a target raw material fish type selected by a user from the fermentation enzyme type set and the raw material fish type set by using the parameter input unit; and searching for a target production parameter group in the fitting database by using the target fermentation enzyme type and the target raw material fish type. The application mainly aims to solve the problems of inaccurate parameter setting during fish solubles production and energy waste during production of fish solubles.

Disclosed are systems and methods for producing renewable alcohols by fermentative processes using selected promoters that differentially regulate the expression of genes encoding fermentation enzymes during the propagation and production phases of the fermentative processes. These systems and methods are able to increase production efficiency and yield in the conversion of sugars to renewable alcohols by fermentation in microbes. This is accomplished by the use of novel nucleic acid sequences that act as genetic switches to differentially control gene expression based on extra cellular stress; namely, stresses relating to fermentation processes. These sequences present a novel method for controlling endogenous gene expression without the need for the addition of heterlogous chemicals or signaling molecules.

The invention discloses a preparation method of a lactic acid bacteria fermentation extract dairy product, and belongs to the technical field of dairy product processing. The method comprises the following steps: (1) mixing eclipta and glossy privet fruit according to a weight ratio of 1: (0.5-2), adding water, extracting, and concentrating to obtain an eclipta-glossy privet fruit extract; (2) mixing the skimmed milk with the herba ecliptae-glossy privet fruit extract, sterilizing, inoculating lactic acid bacteria, and fermenting until the pH value is 4.2-4.8 to obtain fermentation liquor; (3) sequentially carrying out two-step enzymolysis on the fermentation liquor through pepsin and trypsin to obtain enzymatic hydrolysate; (4) centrifuging the enzymatic hydrolysate, taking supernatant, enabling the supernatant to sequentially pass through ultrafiltration membranes with the molecular weight cut-off of 8kDa and 5kDa, and collecting components with the molecular weight of 5-8kDa; and (5) mixing the obtained components with the dairy product base material and the sweetening agent, homogenizing, and sterilizing to obtain the product. Through combination of fermentation, enzymolysis and ultrafiltration separation, active components in a specific molecular weight interval are enriched, and the prepared dairy product has the effects of inhibiting periodontal pathogenic bacteria and regulating inflammatory factors and can be used for daily prevention and adjuvant therapy of periodontitis.

The invention belongs to the technical field of feed enzymes, and particularly relates to a granulation method of glucose oxidase. The enzyme in the enzyme granules is glucose oxidase, is suitable for feed additives, and is beneficial to animal nutrition and health care. The enzyme powder prepared by mixing the raw materials containing glucoamylase, peach gum, calcium chloride and the like and then spray-drying the mixture has the advantages of partial immobilized enzyme, and the stability is improved. Meanwhile, the surfaces of the enzyme particles are coated with a solution containing xanthan gum and calcium glycinate, the positive reaction is promoted through the calcium glycinate, the effect of catalase in the intestinal tract is inhibited, and the acting effect of the glucose oxidase particles is further improved.

This invention discloses a laccase derived from *Coprinus gracilis*, its recombinant expression strain, and a highly efficient expression method, belonging to the fields of microbiology and bioengineering technology. Using *Aspergillus niger* MA70.15 as the experimental strain, this invention employs a random integration strategy to achieve pC3-P... citA -SP GlaA - lcc9‑ T trpC ‑pyrG The expression vector was integrated into the upstream region of the promoter of the host bacterium citrate synthase in the form of nine consecutive copies. To investigate the effects of microparticles and osmotic regulators in the fermentation medium on laccase production by recombinant Aspergillus niger, this invention added different types and concentrations of microparticles and osmotic regulators, resulting in a laccase activity of 1566.7 U / L in shake-flask fermentation of recombinant strain AnRcgL1 and 1961 U / L in a 1 L fermenter, successfully achieving efficient recombinant expression of laccase Lcc9 in Aspergillus niger.

The present application belongs to the field of microcapsule preparation, and relates to a kind of high all-trans carotenoid microcapsule and its preparation method.The preparation method of the high all-trans carotenoid microcapsule includes high-temperature melting of an oil phase containing carotenoid, carrier oil and emulsifier, shearing emulsification of the obtained carotenoid oil solution and wall material aqueous phase, and then spray drying after isomerization treatment of the obtained emulsion with Blakeslea trispora fermentation enzyme solution, to obtain the high all-trans carotenoid microcapsule.In the present application, the emulsion is subjected to isomerization treatment with Blakeslea trispora fermentation enzyme solution before spray drying, which can significantly increase the content of all-trans structure in carotenoid microcapsule, avoid the use of high-toxicity organic solvents in the production process, reduce the harmful factors to workers' health in the production process, improve product quality, and has wide application prospect.

The utility model is suitable for the technical field of soybean fermentation, and provides soybean fermentation enzymolysis equipment which comprises a box body, a rotating rod is rotationally mounted in the box body; a plurality of spiral frames are fixedly connected to the periphery of the rotating rod, the bottom of the box body is in an arc shape, and the spiral frames make contact with the bottom of the box body when rotating; two fixing pipes are fixedly installed in the box body and located above the spiral frame. A plurality of spray heads are fixedly mounted at the bottom of each fixed pipe; a movable frame is slidably mounted in the box body and located above the fixed pipe. According to the soybean fermentation and enzymolysis equipment provided by the scheme, impurities and wizened soybeans can be screened and discharged, the situation that the impurities and the wizened soybeans influence soybean fermentation and enzymolysis is avoided, the soybeans butted to the bottom can be overturned upwards, the soybeans are conveniently stirred, the soybeans are fully contacted and mixed with enzyme, the soybeans can be fully subjected to fermentation and enzymolysis, and the soybean fermentation and enzymolysis efficiency is improved. And the fermented and enzymolyzed soybeans can be conveniently guided out through the discharge hopper.

The invention relates to the field of synthetic phospholipids, and discloses a method for preparing phosphatidyl ethanolamine synthetic phospholipids under catalysis of phospholipase D. The method comprises the following steps: A, completely dissolving PC in an organic solvent to obtain a PC solution; b, adding ethanolamine into the buffer solution, and adjusting the pH value in an ice bath; c, mixing a metal salt solution with phospholipase D to obtain a fermentation enzyme solution, pouring a buffer solution containing ethanolamine, and uniformly mixing to obtain a water phase; d, mixing the PC solution with a water phase to uniformly disperse PC, and carrying out hydrolysis reaction to obtain a PE crude product reaction solution; e, standing for layering to obtain a water layer, extracting the water layer, and concentrating the obtained organic layer to obtain a PE crude product concentrate; f, refining to obtain a PE crude product I; g, performing column chromatography separation and vacuum concentration to obtain a PE crude product II with the purity not lower than 98%; f, drying to obtain a PE finished product. The method is simple in process, good in enzyme specificity, mild in reaction condition, environment-friendly, high in yield and beneficial to large-scale production of enterprises.

The present application belongs to the field of biotechnology, and particularly relates to a method for preparing a compound pentapeptide with lipid-lowering and glucose-lowering functions by using enzymolysis-fermentation-enzymolysis technology and application thereof. The present application provides a compound pentapeptide with lipid-lowering and glucose-lowering functions, which is obtained by specifically combining soybean, highland barley, mulberry leaves, ginseng and tricholoma matsutake, and can synergistically improve the lipid-lowering and glucose-lowering effects. In addition, the present application also provides a scheme for preparing polypeptides by using the triple biotransformation technology of enzymolysis-fermentation-enzymolysis combined process, which is beneficial to decomposing plant macromolecular substances into small molecular nutrient components which are easy to absorb, and simultaneously extracting the five-dimensional plant compound pentapeptide with glucose-lowering and lipid-lowering functions, thereby improving the biological activity and functionality of the compound pentapeptide, and providing high-quality raw materials for the prevention and treatment of diabetes and hyperlipidemia. Therefore, the technical scheme of the present application has the advantages of safety and reliability, good effect and no side effects.

The application discloses a kind of composite enzyme, cellulase, xylosolase, papain are composed according to mass ratio 2-4:1-5:0.5-0.7.The use method of the composite enzyme in the manufacture of water-soluble fertilizer, comprising the following steps: (1) soybean is made into soybean meal powder, standby;(2) after mixing raw materials and stirring uniformly, fermentation enzymolysis is carried out, and fermentation enzymolysis liquid is prepared;(3) the fermentation enzymolysis liquid prepared in step (2) is filtered using filter tank and then placed in filter press for solid-liquid separation, and filtrate is prepared;(4) the filtrate prepared in step (3) is placed in inactivation device for inactivation treatment, and water-soluble fertilizer is prepared.The water-soluble fertilizer product produced by the application contains rich plant-derived amino acid small peptides, polypeptides, oligosaccharides and other active elements and various chelated trace elements, no waste residue is generated during preparation, no emissions, low energy consumption, simple and easy-to-control production process, easy to form scale production, which is beneficial to cost reduction and efficiency improvement.

The application provides a preparation method of functional protein for shrimp feed and the functional protein and application, wherein the steps of the preparation method of the application include three steps of raw material compounding, fermentation and enzymolysis and post-treatment. The raw material compounding is uniformly mixing composite plant protein raw materials containing corn gluten meal, cottonseed protein and peanut meal to obtain a mixture; the fermentation and enzymolysis is inoculating composite bacteria liquid containing lactobacillus plantarum and bacillus subtilis into the mixture, adding composite enzyme preparation containing protease, phytase, pectinase, hemicellulase and cellulase, and then performing solid-state fermentation and enzymolysis after adjusting the moisture content of the material to obtain a fermentation product; and the post-treatment is drying and crushing the fermentation product to finally obtain the functional protein synergized by bacteria and enzymes. Adding the prepared functional protein to low-fish meal shrimp feed can reduce the inhibition of protein synthesis and metabolism of shrimp caused by low-fish meal feeding, improve the intestinal digestion capacity of shrimp and the antioxidant level of the body, and thus optimize the overall growth performance of shrimp.

The invention relates to the technical field of seasonings, and particularly discloses a method for preparing a high-freshness base material based on high-protein raw materials. The method comprises the following steps: puffing a high-protein raw material to obtain a puffed high-protein raw material; adding water into the puffed high-protein raw material for wetting, then preheating with steam, and then cooling and drying; inoculating aspergillus oryzae and aspergillus oryzae protease into the high-protein raw material, and performing koji making to obtain a koji material; uniformly mixing the koji, the enzymatic hydrolysate and edible salt water, and fermenting to obtain the high-freshness base material. According to the method, the high-protein raw materials are subjected to puffing treatment, enzymes are added in the starter propagation process, so that the aspergillus oryzae generates more enzymes and preliminarily decomposes macromolecular proteins, the high-freshness base material is obtained through fermentation and enzymolysis, and the method is high in enzymolysis efficiency, high in high-protein raw material utilization rate and capable of generating more umami substances.

The invention provides a preparation method of a brown sugar fermentation product capable of improving saline-alkaline resistance of crops as well as a product and application of the brown sugar fermentation product. The method comprises the following steps: mixing brown sugar, fermentation enzyme and a buffer solution, inoculating zymophyte to perform two-stage fermentation, performing solid-liquid separation, and collecting liquid to obtain the brown sugar fermentation product, the fermentation enzyme comprises any one or a combination of at least two of cellulase, amylase or protease; the zymophyte comprises any one of bacillus subtilis, bacillus licheniformis or bacillus amyloliquefaciens or a combination of at least two of the bacillus subtilis, the bacillus licheniformis and the bacillus amyloliquefaciens. The brown sugar fermentation product prepared by the method disclosed by the invention can obviously improve the biomass of crops, improve photosynthesis and moisture state, maintain salt ion balance and relieve oxidative stress induced by salt and alkali.

The present invention belongs to the technical field of traditional Chinese medicine, and specifically relates to a traditional Chinese medicine fermentation composition for relieving visual fatigue and a preparation method thereof. The present invention discloses a preparation method of the traditional Chinese medicine fermentation composition for relieving visual fatigue, comprising: using a composite enzyme to hydrolyze the traditional Chinese medicine components, and then fermenting the hydrolyzed traditional Chinese medicine components with a mixed bacteria composed of Lactobacillus rhamnosus, Saccharomyces cerevisiae and Aspergillus niger. The prepared traditional Chinese medicine fermentation composition has been verified to be safe, non-irritating and has better efficacy; and the massage cream prepared using the traditional Chinese medicine fermentation composition has a significant effect of relieving visual fatigue. The preparation method of the traditional Chinese medicine fermentation composition described in this application is simple, can solve the problems of cumbersome and complicated, long treatment course and slow effect in the existing traditional Chinese medicine treatment of visual fatigue, and has good clinical application value.

The present invention belongs to the field of biochemical production, relates to strain mutation and strain selection method of microbial fermentation, and is specifically related to a strain of Bacillus pumilus producing alkaline cellulase and its application. The strain is obtained after ultraviolet mutagenesis and diethyl sulfate mutagenesis breeding, specifically Bacillus pumilus WS-135, and the deposit number is CGMCC NO.18641. The alkaline cellulase produced by the strain is heat-resistant, alkali-resistant, high in enzyme activity, and stable in performance. At the same time, the present invention also optimizes the corresponding fermentation mechanism, and the fermentation mechanism has higher fermentation activity, higher extraction yield, lower manufacturing cost, and fermentation enzyme activity is on average more than 360U / mL; and a spray drying method for alkaline cellulase is developed, and a spraying method for an alkaline cellulase solid enzyme preparation is provided. The existing alkaline cellulase preservation and the inconvenient transportation are solved, and it has a wide range of application prospects.