Method for preparing chitosan through composite fermentation and enzymatic hydrolysis

A technology of chitosan and compound fermentation, applied in the field of manufacturing, can solve the problems of high concentration of lye, waste, inability to recycle, etc., and achieve the effects of low energy consumption and improved production efficiency

Active Publication Date: 2014-09-17
TIANJIN TIANSHI BIOLOGICAL DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the high concentration of lye used in the chemical method, the reaction time is long, the product quality is unstable, and the environmental pollution is serious
Chitosan prepared by traditional chemical methods after decalcification and deproteinization is discarded and cannot be recycled, resulting in waste

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Dry and crush crab or shrimp shells to 0.5mm particles, add 4 times the weight-to-volume ratio of water to make a feed liquid, add Bifidobacterium lactis: Lactobacillus bulgaricus: Lactobacillus helveticus = 2:6:5 lactic acid bacteria, lactic acid bacteria accounted for The weight ratio of raw materials is 0.02%, ferment for 36h, then 70-100°C, 20min; fermented liquid is added with bromelain and papain with a weight ratio of 1:2 compound protease, the weight ratio of protease to shell material is 0.05%, and citric acid is added to adjust the pH When the temperature reaches 5.5, stir at 40°C for 1h; separate the liquid, extract the residue again, filter, discard the filtrate, keep the residue, add 40% sodium hydroxide solution, wash at 60°C for 1.5h, wash with water until neutral, and dry to obtain several Tetracan.

Embodiment 2

[0026] Dry and crush crab or shrimp shells to 1.0mm particles, add 8 times the weight-to-volume ratio of water to make a feed liquid, add Bifidobacterium lactis: Lactobacillus bulgaricus: Lactobacillus helveticus = 2:6:5 lactic acid bacteria, lactic acid bacteria account for The weight ratio of raw materials is 0.06%, and the fermented liquid is added with bromelain and papain with a weight ratio of 1: 5 composite protease, and the weight ratio of protease to the shell material is 0.2%, and citric acid is added to adjust the pH value to 6.5, and stir at 60°C for 2 hours; separate the liquid , the residue was extracted again, filtered, the filtrate was discarded, and the residue was retained, added with 60% sodium hydroxide solution, washed at 90°C for 4 hours, washed with water until neutral, and dried to obtain chitosan.

Embodiment 3

[0028] Dry and crush the crab or shrimp shells to 0.5mm particles, add 8 times the weight-volume ratio of water to make a feed liquid, add Bifidobacterium lactis: Lactobacillus bulgaricus: Lactobacillus helveticus = 2:6:5 lactic acid bacteria, lactic acid bacteria accounted for The weight ratio of raw materials is 0.04%, and the fermented liquid is added with bromelain and papain in a weight ratio of 1: 5. The protease accounts for 0.1% of the shell material weight ratio. Add citric acid to adjust the pH value to 6.5, and stir at 60°C for 2 hours; separate the liquid , the residue was extracted again, filtered, the filtrate was discarded, the residue was retained, and 60% sodium hydroxide solution was added, at 90°C for 4 hours, washed with water until neutral, and dried to obtain chitosan.

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PUM

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Abstract

The invention discloses a method for preparing chitosan through composite fermentation and enzymatic hydrolysis. The method comprises the following steps: drying crab shell or shrimp shell, and crushing; fermenting; carrying out enzymatic hydrolysis calcium removal and protein removal; and carrying out enzymatic hydrolysis for acetyl removal, washing with water, drying, and crushing. Compared with the prior art, the above enzymatic hydrolysis method has the advantages of realization of calcium removal and protein removal in a same step, substantial improvement of the production efficiency, no use of a strong aid, low energy consumption, no pollution and environmental protection. A free calcium solution obtained after the enzymatic hydrolysis can be recovered to prepare an organic calcium preparation, so all products can be reasonably used to generate substantial economic values.

Description

technical field [0001] The invention relates to a preparation method of chitosan and belongs to the field of manufacturing. Background technique [0002] Chitin is a natural nitrogen-containing polysaccharide substance, which widely exists in the shell or cuticle of invertebrates, especially in the shells of shrimp and crabs. Chitin is easy to obtain and renewable. It is a natural homopolymer second only to cellulose. However, due to the strong hydrogen bond in the molecule, the solubility is low and the application range is limited. [0003] Chitosan is formed after deacetylation of chitin. At present, chitosan is usually prepared by chemical and enzymatic deacetylation. In the chemical deacetylation reaction, there are many factors affecting the deacetylation, but the main influencing factors include: lye concentration, reaction temperature and time. In general, increasing the reaction temperature and prolonging the reaction time can increase the degree of deacetylatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
Inventor 王丹宇郁正刚杨之光朱旭赵宁芳胡巧凤陶会会
Owner TIANJIN TIANSHI BIOLOGICAL DEV
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