ª—- carrageenin catabolic enzymes , its preparing process and application

A carrageenan and degrading enzyme technology, applied in the field of kappa-carrageenan degrading enzymes, can solve the problems of easily damaged active groups, unstable product quality, severe reaction conditions, etc., and achieves simple preparation process, stable quality, and good product quality. high rate effect

Inactive Publication Date: 2004-11-10
OCEAN UNIV OF CHINA
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Problems solved by technology

[0002] At present, there are reports on the preparation of carrageenan oligosaccharides by chemical methods, but thi...

Method used

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Embodiment Construction

[0008] The κ-carrageenan degrading enzyme of the present invention can be prepared by liquid fermentation of marine cellulophage. During preparation, after activating the strain with 2216E medium, insert it into a 250ml Erlenmeyer flask containing 100ml of medium with a 5% inoculum size, and after cultivating the shake flask at 28-35°C for 32-40h, the culture solution was added at 4000rpm Refrigerated and centrifuged for 10 minutes to remove the bacterial cells to obtain the fermented enzyme liquid. After the enzyme solution was concentrated by ultrafiltration, it was mixed with 40% (NH 4 ) 2 SO 4 Remove impurity proteins, after refrigerated centrifugation, continue to add (NH 4 ) 2 SO 4 To 80% saturation, freeze and centrifuge to collect the precipitate, put it into a dialysis bag for dialysis for 48 hours, and freeze-dry to prepare the κ-carrageenan degrading enzyme of the present invention. The enzyme can be used to prepare carrageenan oligosaccharides. κ-carrageenan...

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Abstract

The invention is an enzyme, and its characteristic: it can degrade k-carrageenan to prepare oligo-carrageenan and degrade beta-1, 4-indican bond of k-carrageenan, therefore called k-carrageenan degrading enzyme, its molecular weight is 30,000Da. When preparing it, using 2216E culture medium to make shaking bottle culture on the sea Cytophaga sp at 28-35deg.C, then centrifugating the culture solution to obtain fermentation enzyme solution, ultrafiltering and concentrating the enzyme solution, then using 40-80% (NH4)2SO4 for salting-out and collecting protein deposit, and freeze-drying. It can effectively degrade k-carrageenan, be used to prepare oligo-carrageenan, and compared with chemical method, it has simple preparing coursee, high product yield, stable quality, and ensures the activity research and development of oligosaccharide, etc.

Description

technical field [0001] The invention relates to an enzyme, in particular to a κ-carrageenan degrading enzyme which can be used to prepare carrageenan oligosaccharides. Background technique [0002] At present, there are reports on the preparation of carrageenan oligosaccharides by chemical methods, but this method has disadvantages such as severe reaction conditions, easily damaged active groups, and unstable product quality. Contents of the invention [0003] The purpose of the present invention is to provide a kind of kappa-carrageenan degrading enzyme, it can be used in the preparation of carrageenan oligosaccharide, can overcome the above-mentioned shortcoming of prior art. [0004] An enzyme characterized in that it can degrade κ-carrageenan, used to prepare carrageenan oligosaccharides, and can degrade the β-1,4-glucosidic bond of κ-carrageenan, so it is also called κ-carrageenan degradation Enzyme, the molecular weight of the enzyme is 30,000Da. [0005] A prepara...

Claims

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Application Information

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IPC IPC(8): C12N9/42C12P19/00
Inventor 牟海津江晓路管华诗
Owner OCEAN UNIV OF CHINA
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