Method for producing tobacco leaf fermenting enzyme preparation

A technology of enzyme preparation and compound enzyme preparation, applied in the directions of biochemical equipment and methods, enzymes, enzymes, etc., can solve the problem that the requirements of mass production of cigarette enterprises are not described, so as to improve the application effect, exert the potential quality of tobacco leaves, enhance the The effect of natural aroma

Inactive Publication Date: 2008-03-19
谢勇 +2
View PDF3 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] A common feature of the above documents and patents is that exogenous (micro) biological enzymes are directly mixed in a specific ratio and then ap

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing tobacco leaf fermenting enzyme preparation
  • Method for producing tobacco leaf fermenting enzyme preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0046] Implementation (see Figure 3)

[0047] 1. Select B2F, a representative tobacco leaf from the upper part of Yun 85 in the Hongta area of ​​Yuxi;

[0048] 2. Prepare a 0.4% solution according to the instructions of the enzyme preparation, spray the B2F smoke sample evenly at room temperature, and alcoholize at a temperature of 28°C and a relative humidity of 65%; (A)

[0049] 3. Simulate the re-baking process conditions (75±5°C), process the smoke samples according to the method in 1, store them at 28°C (B1) and 38°C (B2) respectively, and carry out alcoholization under the condition of relative humidity of 65%;

[0050] 4. Conduct professional smoking evaluation, smoking machine analysis and routine chemical analysis on (A), (B1) and (B2) smoke samples after 60 days;

[0051] 5. Use MATLAB statistical analysis software for data analysis.

[0052] The results of sensory evaluation showed that (A) method significantly improved the quality of tobacco leaves, showing that ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention relates to a novel tobacco leaf fermenting enzyme preparation production method which aims to solve the technical problems that how the quality of the tobacco leaf fermenting product is improved and how the reactivity protection of the tobacco leaf fermenting enzyme preparation is realized in the natural fermenting field of the tobacco leaf. The enzyme preparation consists of glucoseoxidase, chlorophyl oxidase, carotenoid oxidase, protease, and nicotine-degradation enzyme. Through the cell disruption of fresh leaves, (NH 4) 2 SO 4 is utilized to operate the second fractional precipitation to obtain crude enzyme fluid, an enzyme molecule adopts Ca 2 + and Mg 2 + to operate the metal ion exchange, to accomplish the molecule modification; a macro molecule combination modification is accomplished through adopting 0.01 percent of cane sugar low molecular polymer, thereby prolonging the half life period of an enzyme preparation, and obviously improving the high temperature resistant ability. The experimental result employed by the enzyme preparation indicates that the nicotine is decreased by 9.3 percent, the total nitrogen is decreased by 5.7 percent, the protein is decreased by 7.1 percent; cigarette smoke condensates are decreased by 8.4 percent, the tar content is decreased by 5.1 percent, cigarette smoke nicotine content is decreased by 28.0 percent, and the carbon is decreased monoxide by 1.6 percent. The enzyme preparation is employed when the tobacco is wet for the second time before defolat and redrying.

Description

technical field [0001] Tobacco leaves ferment naturally. Background technique [0002] In the process of tobacco leaf modulation, fully fermenting the tobacco leaves after primary roasting and re-curing under suitable temperature and humidity conditions is an extremely important technical link to improve and improve the quality of tobacco leaves. Therefore, the research on the fermentation mechanism has been the focus of domestic and foreign scholars and cigarette manufacturers for many years. One of the important research areas is the elucidation and application of the enzyme mechanism of tobacco leaf fermentation. As early as 1950 (Frankenburg, W.G), some scholars proposed the theoretical framework that tobacco fermentation was catalyzed by enzymes. With the deepening and accumulation of research, it is now generally believed that the oxidoreductases contained in tobacco leaves themselves dominate the fermentation, and this fermentation process is the result of the joint...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N9/98A24B15/20A24B15/28
Inventor 谢勇韩伟陈应昆何宝玉
Owner 谢勇
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap