The present invention relates to a novel tobacco fermenting
enzyme preparation production method. The purpose is to solve the technical problems that how improve the quality of the tobacco fermenting product is improved and how the reactivity protection of the tobacco fermenting
enzyme preparation is realized in the natural fermenting field of tobacco. The
enzyme preparation consists of a glucoseoxidase, a chlorophyl oxidase, a
carotenoid oxidase, a
protease, and a
nicotine-degradation enzyme. Through the
cell disruption of fresh leaves, (NH 4) 2 SO 4 is utilized to operate the second
fractional precipitation to obtain crude enzyme fluid, an enzyme molecule adopts Ca 2 + and Mg 2 + to operate the
metal ion exchange, to accomplish the molecule modification; a
macro molecule combination modification is accomplished through adopting 0.01 percent of
cane sugar low molecular
polymer, thereby prolonging the
half life period of theenzyme preparation and obviously improving the high temperature resistant ability. The experimental result employed by the enzyme preparation indicates that the
nicotine is decreased by 9.3 percent, the
total nitrogen is decreased by 5.7 percent, the
protein is decreased by 7.1 percent;
cigarette smoke condensates are decreased by 8.4 percent, the
tar content is decreased by 5.1 percent, the
cigarette smoke nicotine content is decreased by 28.0 percent, and the
carbon monoxide is decreased by 1.6 percent. The enzyme preparation is employed when the
tobacco leaf is wet for the second time before defolat and redrying.