Method for producing tobacco leaf fermenting enzyme preparation

Abstract

The present invention relates to a novel tobacco fermenting enzyme preparation production method. The purpose is to solve the technical problems that how improve the quality of the tobacco fermenting product is improved and how the reactivity protection of the tobacco fermenting enzyme preparation is realized in the natural fermenting field of tobacco. The enzyme preparation consists of a glucoseoxidase, a chlorophyl oxidase, a carotenoid oxidase, a protease, and a nicotine-degradation enzyme. Through the cell disruption of fresh leaves, (NH ₄) ₂ SO ₄ is utilized to operate the second fractional precipitation to obtain crude enzyme fluid, an enzyme molecule adopts Ca ^{2 +} and Mg ^{2 +} to operate the metal ion exchange, to accomplish the molecule modification; a macro molecule combination modification is accomplished through adopting 0.01 percent of cane sugar low molecular polymer, thereby prolonging the half life period of theenzyme preparation and obviously improving the high temperature resistant ability. The experimental result employed by the enzyme preparation indicates that the nicotine is decreased by 9.3 percent, the total nitrogen is decreased by 5.7 percent, the protein is decreased by 7.1 percent; cigarette smoke condensates are decreased by 8.4 percent, the tar content is decreased by 5.1 percent, the cigarette smoke nicotine content is decreased by 28.0 percent, and the carbon monoxide is decreased by 1.6 percent. The enzyme preparation is employed when the tobacco leaf is wet for the second time before defolat and redrying.

Description

technical field [0001] Tobacco leaves ferment naturally. Background technique [0002] In the process of tobacco leaf modulation, fully fermenting the tobacco leaves after primary roasting and re-curing under suitable temperature and humidity conditions is an extremely important technical link to improve and improve the quality of tobacco leaves. Therefore, the research on the fermentation mechanism has been the focus of domestic and foreign scholars and cigarette manufacturers for many years. One of the important research areas is the elucidation and application of the enzyme mechanism of tobacco leaf fermentation. As early as 1950 (Frankenburg, W.G), some scholars proposed the theoretical framework that tobacco fermentation is catalyzed by enzymes. With the deepening and accumulation of research, it is now generally believed that the oxidoreductases contained in tobacco leaves themselves dominate the fermentation, and this fermentation process is the result of the joint ...

AI Technical Summary

Problems solved by technology

[0005] A common feature of the above documents and patents is that exogenous (micro) biological enzymes are directly mixed in a specific ratio and then applied manually. There is no description on how to protect the enzyme activity and how to meet the requirements of mass production of cigarette enterprises.

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