Method for producing forage plant protein by carrying out enzyme synergy fermentation and enzymolysis on plant protein

A technology for enzymatic hydrolysis of plant protein and plant protein, applied in animal feed, animal feed, applications, etc., to achieve the effects of cost reduction, easy control of product technology, and good enzymatic hydrolysis

Active Publication Date: 2014-10-01
湖北邦之德牧业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0023] The object of the present invention is to provide a method for producing forage plant protein by enzymolyzing plant protein through enzyme-bacteria synergistic fermentation, to solve the problems in the industrialization of fermented protein, including bacterial strain combination,

Method used

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Examples

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Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: is a basic embodiment of the present invention. A method for producing fodder vegetable protein by co-fermentative enzymolysis of plant protein with enzymatic bacteria, the enzymatic bacteria used for fermentative enzymolysis include compound enzyme preparations and microbial strains; the vegetable protein raw materials used for fermentative enzymatic hydrolysis are selected from two or The remaining meal or dregs after pressing or fermenting, extracting and processing two or more kinds of plant seeds; the steps of the method for producing protein for feed include: batching, inoculation and mixing, fermentation and enzymolysis, drying, cooling, crushing, product testing, and finally obtained feed protein The small peptide content of plant protein products is 10-40% of the protein contained.

Embodiment 2

[0043] Example 2:It is a further embodiment of embodiment 1. In the present invention, a method for producing forage vegetable protein by co-fermenting and enzymatically hydrolyzing plant protein with enzyme bacteria, the remaining meal or dregs after the plant seeds are pressed or fermented and extracted include soybean meal, cottonseed meal, rapeseed meal, distiller's grain .

Embodiment 3

[0044] Example 3: It is a further embodiment of embodiment 1. A method for producing forage vegetable protein by enzymatically hydrolyzing vegetable protein with enzymobacteria synergistic fermentation of the present invention, the steps are:

[0045] A. Ingredients: the vegetable protein raw material used for fermented enzymolysis is selected from soybean meal, cottonseed meal, rapeseed meal, distiller's grains, which is a combination of two or more; B. Inoculation and mixing: the enzyme bacteria used for fermentation and enzymolysis are compound enzyme preparations, Bacillus subtilis BZD-01, and Saccharomyces cerevisiae BZD-02, and these three enzyme bacteria are mixed with the vegetable protein raw materials in step A to form a mixed material; C. Fermentation enzymolysis: control the temperature and oxygen of the mixed material in step B, and use the secondary temperature to control the fermentation enzymolysis to obtain the fermentation enzymolyzate; D. Drying: perform...

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Abstract

The invention relates to a method for producing a forage plant protein by carrying out enzyme synergy fermentation and enzymolysis on a plant protein. The method is characterized in that enzyme for the fermentation and the enzymolysis comprises a complex enzyme preparation and microbial strains; a plant protein raw material for the fermentation and the enzymolysis is extracted from residual processed meal or grains after two or more kinds of plant seeds are compressed or fermented. The method comprises the steps of preparing the materials, inoculating and mixing the materials, carrying out the fermentation, carrying out the enzymolysis, drying, cooling, crushing and carrying out product detection, so as to finally obtain a forage plant protein product, wherein the content of small peptide of the forage plant protein product is 10-40% of that of the proteins of the forage plant protein product. The method has the advantages that the key technical problems of high labor intensity, difficulty in control, instable quality, low production efficiency, high energy consumption and the like in the solid state fermentation process of the plant proteins can be solved, the industrialization of a fermented plant protein product can be effectively promoted, and the economic benefit can be increased.

Description

technical field [0001] The invention relates to a method for producing forage plant protein by enzymolyzing plant protein through co-fermentation with enzyme bacteria, which belongs to the field of bioengineering. Specifically, it is an industrial engineering technology that uses the combined enzyme preparation, Bacillus subtilis BZD-01, and brewer's yeast BZD-02 to convert plant protein into fermented and enzymatically hydrolyzed technology to produce high-quality protein for feed. Background technique [0002] 1. Feed farming industry’s demand for high-quality plant protein: [0003] Feed farming is one of the pillar industries in many countries, so the total demand for feed is huge; [0004] The development of the feed industry must rely on high-quality raw materials, especially protein raw materials. But there are following problems: [0005] (1) The shortage of animal protein resources and the existence of safety problems; [0006] (2) Soybean meal cannot meet the d...

Claims

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Application Information

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IPC IPC(8): A23K1/18A23K1/14A23K1/16A23K1/165A23K10/12A23K10/14A23K10/18A23K10/37A23K10/38A23K20/147
CPCY02P60/87
Inventor 吴晓峰葛向阳张涛胡敏苏树和廖媛媛李苏
Owner 湖北邦之德牧业科技有限公司
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