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Production of alcohol from mixed bacterial population degradable fermented bastose substance

A lignocellulosic, mixed bacterial group technology, applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions, can solve the problems of large amount of enzymes, long production cycle and high production cost

Inactive Publication Date: 2011-07-20
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of high production cost, long production cycle, and large amount of enzymes in the production of ethanol by using cellulose substances, and provide a method for producing ethanol by degrading and fermenting lignocellulosic substances with mixed flora

Method used

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specific Embodiment approach 1

[0005] Specific embodiment one: the present embodiment degrades and ferments lignocellulosic substances to produce ethanol according to the following steps: (1) after lignocellulosic substances are pulverized, use concentration of 0.5 to 1.5% H 2 SO 4 Soak for 11-13 hours, then steam explosion for 6-10 minutes under the conditions of 1.6-2.0MPa, 180-200℃; 5.5 to 6.5 of the nutrient solution, then high-temperature sterilization to make the saccharification and fermentation substrate; (3) will be in the logarithmic growth phase according to the volume ratio of 1:1, and the colony concentration in the culture solution is 1×10 7 ~9×10 8 Saccharomyces cerevisiae in cfu / mL and cellulose-degrading bacteria that can produce cellulase under the fermentation conditions of Saccharomyces cerevisiae were added to the saccharification fermentation substrate together. 8% to 12% of the volume of the saccharification and fermentation substrate; (4) synchronously degrade the saccharification ...

specific Embodiment approach 2

[0007] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the lignocellulosic substance after pulverizing in the step (1) is that the concentration of 1% H 2 SO 4 Soak for 12 hours, then steam explode for 8 minutes under the conditions of 1.8MPa and 185-195℃. Other steps are the same as those in Embodiment 1.

specific Embodiment approach 3

[0008] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the lignocellulosic material in step (1) is sugarcane residue, rice husk or straw, and the particle size after crushing is 40-60 mesh. Other steps are the same as those in Embodiment 1 or 2.

[0009] The raw materials of lignocellulosic materials used in this embodiment are industrial, agricultural or food processing wastes, turning waste into treasure, improving the recyclable utilization rate of materials, saving grain, sugar cane, corn and other crops, and reducing production costs.

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Abstract

The present invention discloses a method for producing alcohol from mixed microbial pool degradable and fermented lignocellulose substance, relating to a method for producing alcohol by degradation of fermented lignocellulose substance, and solves the problems of high cost and long production cycle in production of alcohol by using the lignocellulose substance in the prior art. The method comprises the following steps: 1) crushing lignocellulose substance, soaking with H2SO4, and bursting by steam; 2) solid-liquid separating, adding nutritive liquid into solid-phase substance, and sterilizing; 3) adding distiller's yeast and cellulose degrading microbe into saccharifying fermentative substrate; 4) degradation saccharifying-fermenting synchronously, distilling and rectifying to obtain final product. The method for producing alcohol from mixed microbial pool degradable and fermented lignocellulose substance provided by the present invention has the advantages that: degradation and saccharification-fermentation are synchronously occurred, thus the degradation and saccharification-fermentation period is shortened by 40% while the productive technology is simplified and the use ratio of equipment is improved. Moreover, the feedback inhibition function to cellulose hydrolysis of monose is eliminated and both the raw material utilization rate and alcohol yield are improved.

Description

technical field [0001] The invention relates to a method for producing alcohol by degrading and fermenting lignocellulosic substances. Background technique [0002] With the increasing shortage of energy and the need for sustainable development, seeking new forms and utilization of renewable energy has become an urgent and realistic research topic all over the world. As a substitute for fossil fuels, fuel ethanol is convenient for storage and transportation, and can be used as vehicle power fuel without changing the existing gas station facilities and engine forms. In addition, the use of alcohol as a motor fuel has excellent environmental benefits and can significantly reduce the amount of greenhouse gases emitted into the atmosphere. Therefore, the amount and scope of application of ethanol is gradually expanding. [0003] At present, the industry mainly uses hexose sugars to produce fuel alcohol through Saccharomyces cerevisiae, mainly using molasses and grains as raw m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/10C12R1/865
CPCY02E50/16Y02E50/10
Inventor 冯玉杰李冬梅
Owner HARBIN INST OF TECH
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