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Method and device for extracting fumaric acid in fermentation broth in continuous counter current manner by means of amine extractants and application of fermentation technology combined with method

A technology of fumaric acid and fermented liquid, which is applied in the field of bioengineering, can solve the problems of unextracted fumaric acid, etc., and achieve the effect of large extraction and separation factor, easy automatic control and scale-up, and beneficial to normal growth and metabolism

Inactive Publication Date: 2015-05-20
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But at present, there are no relevant reports on the online separation of fumaric acid in fermentation broth by extraction and fermentation coupling technology at home and abroad.

Method used

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  • Method and device for extracting fumaric acid in fermentation broth in continuous counter current manner by means of amine extractants and application of fermentation technology combined with method
  • Method and device for extracting fumaric acid in fermentation broth in continuous counter current manner by means of amine extractants and application of fermentation technology combined with method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: Continuous countercurrent extraction of fumaric acid in fermentation broth by amine extractant is used to continuously prepare fumaric acid in coupled fermentation technology.

[0042] (1) Preparation of spore suspension: Rhizopus oryzae ( Rhizopus oryzae ) ME-F13 was inoculated on PDA slant medium (take 200g of fresh peeled potatoes, cut into small pieces, add 1000mL of water, heat and boil for 30min, filter with gauze and make up water loss to 1000mL, get 20% potato extract, add 20g of sucrose , agar 22g, melted and subpackaged, sterilized at 121°C for 20min), the slope was placed in a constant temperature incubator at 35°C for 6 days, after the spores matured, the spores were washed with sterile water under sterile conditions Filtered with a layer of gauze, collected into a conical flask equipped with glass beads, placed in a shaker at 25°C and 150r / min for 30min, and the spore concentration was controlled at 10 7 A / mL spare;

[0043] (2) Fermented se...

Embodiment 2

[0047] Example 2: Continuous countercurrent extraction of fumaric acid in fermentation broth by amine extractant is used to continuously prepare fumaric acid in coupled fermentation technology.

[0048] (1) Prepare spore liquid and fermented seeds according to the method in Example 1.

[0049] (2) Fermentation culture: 3L of fermentation medium (glucose 80g / L, urea 0.1g / L, KH 2 PO 4 0.4g / L, MgSO 4 ·7H 2 O 0.5g / L, ZnSO 4 ·7H 2 O 0.0176g / L, FeSO 4 ·7H 2 O 0.000498g / L), sterilized at 115°C for 30min, after the tank was cooled to room temperature, fermented seeds were added, the inoculation amount was 8% (v / v), the fermenter was placed at a temperature of 33°C, an air flow of 1.2 vvm, and a rotation speed of The fermentation culture was carried out under the operating condition of 300 rpm, and the pH was adjusted to 5.0 by 3.0 mol / L sodium carbonate. After 36 hours of fermentation, the growth of Rhizopus oryzae entered the late stage of logarithmic growth. The concentra...

Embodiment 3

[0052] Example 3: Continuous countercurrent extraction of fumaric acid in fermented broth with amine extractant coupled with fermentation technology for continuous preparation of fumaric acid.

[0053] (1) Prepare spore liquid and fermented seeds according to the method in Example 1.

[0054] (2) Fermentation culture: 5L of fermentation medium (glucose 100g / L, urea 0.1g / L, KH 2 PO 4 0.5g / L, MgSO 4 ·7H 2 O 0.5g / L, ZnSO 4 ·7H 2 O 0.0176g / L, FeSO 4 ·7H 2 O 0.000498g / L), sterilized at 115°C for 30min, after the tank was cooled to room temperature, fermented seeds were added, the inoculation amount was 15% (v / v) the fermenter was placed at a temperature of 38°C, an air flow of 0.5 vvm, and a rotation speed of The fermentation culture was carried out under the operating condition of 600 rpm, and the pH was adjusted to 6.0 by 1.0 mol / L sodium hydroxide. After 20 hours of fermentation, the growth of Rhizopus oryzae entered the mid-logarithmic growth phase. The concentration...

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Abstract

The invention provides a method for extracting fumaric acid in fermentation broth in a continuous counter current manner by means of amine extractants. The method includes that filtered fermentation broth flow is added into an extracting tower to perform liquid-liquid extraction in the continuous counter current manner, the fermentation broth is used as heavy phase, the extraction system is used as light phase, extracts left the extraction tower is subjected to reextraction to generate fumaric acid or fumaric acid salt by reextraction, and the extraction system after reextraction returns the extraction tower and is used circularly; meanwhile, the extract can successively pass a primary organic solvent collection tank and a secondary organic solvent collection tank for secondary treatment, and finally the extracts flowing out returns the fermentation for reusing. The invention further provides a device for extracting fumaric acid in fermentation broth in a continuous counter current manner by means of amine extractants and application of a fermentation technology combined with the continuous counter-current extraction technology in continuous preparation of the fumaric acid. By the method, suppression to products in the process of fermentation of the fumaric acid can be effectively eliminated, and meanwhile, toxicity of the extracts to rhizopus oryzae can be avoided, and fermentation production level of the fumaric acid can be improved.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and specifically relates to a method for continuously countercurrently extracting fumaric acid in a fermentation broth with an amine extractant, and a device for realizing the method, and at the same time relates to the continuous preparation of fumaric acid in the continuous preparation of fumaric acid by using this extraction method coupled with fermentation technology. The application and application method of malic acid. Background technique [0002] Fumaric acid, also known as fumaric acid, is an important dibasic organic carboxylic acid. As an important organic chemical raw material and fine chemical product, it is widely used in the fields of chemical industry, materials, medicine and food. However, the application of fumaric acid in the food field is limited due to the cost increase and environmental pollution of chemically synthesized fumaric acid. Fumaric acid is prepared by fer...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C57/15C07C51/48B01D11/04C12P7/46C12R1/69
Inventor 黄和李霜何四龙徐晴顾帅周峰
Owner NANJING TECH UNIV
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