Device and technique for producing propanoic acid by coupling of fibrous bed bioreactor with extraction separation

A technology of reactor and fiber bed, which is applied in the field of equipment for producing propionic acid coupled with fiber bed reactor and extraction separation, can solve the problems of slow growth of propionic acid production, achieve the elimination of feedback inhibition, improve extraction efficiency, equipment and process operation simple effect

Inactive Publication Date: 2008-05-21
NANJING UNIV OF TECH
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Problems solved by technology

A set of fiber bed reactor constructed by Yang ST et al., the output of propionic acid reaches 71.8g / l (production efficiency 0.08g / l / h), which is the highest value of immobilized production of propionic acid. Similar to other immobilization methods, fiber The fermentation of propionic acid in bed reactor can improve the tolerance of propionic acid to a certain extent, but the production of propionic acid increases slowly under the condition of high concentration of propionic acid

Method used

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  • Device and technique for producing propanoic acid by coupling of fibrous bed bioreactor with extraction separation

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Embodiment 1

[0043] Propionibacterium freudeenreichii NX-4 (Propionibacterium freudeenreichii NX-4, patent number: 200710020579.8, published on October 3, 2007) seed solution with 5% (v / v) inoculation amount Inoculate in the reactor 9 pre-filled with 3L seed medium, the temperature is 30°C, and 0.05L / min N is passed through the inert gas inlet 4 2 At the same time, the constant flow pump 5-1 was used to add 8 mol / l sodium hydroxide aqueous solution to the reactor 9 to control the pH 6.0, and the static culture was carried out for 48 hours to realize the adsorption and fixation of the bacterial strain on the plant fiber (gauze) 8. Replace the seed liquid in the reactor 9 with the fermentation medium, the initial pH is 6.9, use the constant flow pump 5-1 to add 8 mol / l sodium hydroxide aqueous solution to the reactor 9 to control the pH 6.0, and the initial glucose concentration is 50g / l That is, glucose is added to the fermentation medium, and 0.1L / min N 2 , culture temperature 30 ℃, stati...

Embodiment 2

[0045] Insert the P. frescheri NX-4 seed liquid into the reactor 9 pre-installed with 3L seed culture medium with an inoculum size of 10% (v / v), at a temperature of 35° C., feed 0.2 L / min N 2 , use the stirring device 10-1 to stir (rotating speed 200r / min), and use the constant flow pump 5-1 to add 3mol / l sodium hydroxide aqueous solution to control pH 6.0 at the same time, cultivate 36h to realize the cells on the plant fiber 8 (cotton cloth) fixed. Replace the seed liquid in the reactor 9 with the fermentation medium, the initial pH is 6.9, and at the same time use the constant flow pump 5-1 to add 3 mol / l sodium hydroxide aqueous solution to control the pH 6.0, the initial glucose concentration is 120g / l, and the temperature is 35°C , into 0.2L / min N 2 , using the stirring device 10-1 to stir (rotating speed 200r / min), ferment for 150h, the output of propionic acid is 57.5g / l, and the production efficiency reaches 0.38g / l / h.

Embodiment 3

[0047] Insert the P. freudenii NX-4 seed liquid into the reactor 9 pre-installed with 3L seed medium with an inoculum size of 15% (v / v), at a temperature of 37°C, feed 0.3L from the inert gas inlet 4 / min N 2 , using the stirring device 10-1 to stir (rotating speed 400r / min), while using the constant flow pump 5-1 to add 5mol / l sodium bicarbonate aqueous solution to the reactor 9 to control the pH 6.0, and cultivate for 30h to realize the cells in the animal fiber ( Wool fabric) 8 fastening. The seed liquid in the reactor 9 is replaced by the fermentation medium, and the initial pH is 6.9, and at the same time, the constant flow pump 5-1 is used to add 5 mol / l sodium bicarbonate aqueous solution to control the pH 6.0, and the initial glucose concentration is 80g / l. Gas inlet 4 into 0.1L / min N 2 , using the stirring device 10-1 to stir (rotating speed 400r / min), after cultivating for 80h, the fermentation liquid is pumped into the membrane module 14 (alumina inorganic ceramic...

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Abstract

The invention discloses a fibrous bed reactor and the equipment and process for extracting separation and coupling production of propionic acid. The invention utilizes an immobilized fibrous bed reactor unit, a membrane separation unit, an extraction and anti-extraction unit and an elution and condensation unit to realize the efficient production of propionic acid. The device has strong feasibility, simple operation, easy realization of automation, immobilized materials, long using life of the membrane and the matching equipment, convenient maintenance and good application prospect. Through extracting separation and coupling production technology, the separation of propionic acid is realized. The invention radically eliminates the feedback inhibition of propionic acid, implements the high density fermentation of propionibacterium and greatly improves the production efficiency of propionic acid through the fermentation method. The extracted water phase is returned to the reactor for recycle after elution and condensation by an elution device, which eliminates the harm of extractant for propionibacterium from the root.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering and separation engineering, and relates to a device and process for producing propionic acid coupled with a fiber bed reactor and extraction and separation. Background technique [0002] Propionic acid is an important fine chemical product and basic chemical raw material, which is widely used in food, feed, rubber, plastic, paint, coating, fragrance, medicine, pesticide, printing and other fields. At present, the industrial production of propionic acid adopts the chemical synthesis method using petroleum as raw material. For a long time, due to the harsh conditions and technical difficulties of propionic acid production, my country has not been able to achieve large-scale production, mainly relying on imports; on the other hand, due to The world's oil price has been rising all the way, and the production cost of propionic acid using oil as raw material continues to rise. In the lo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00B01D11/00C07C51/48
Inventor 徐虹冯小海姚忠李莎欧阳平凯
Owner NANJING UNIV OF TECH
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