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Strain for high-temperature composting of sludge and application thereof

A composting and bacterial agent technology, applied in the field of microbial fermentation technology and environmental protection, can solve the problems of high power consumption, reduced treatment efficiency, and more odor, and achieves high temperature for a long time, complete degradation of organic matter, ammonia gas, etc. Odor reduction effect

Active Publication Date: 2016-12-07
中泥(北京)环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Alkali stabilization is only used for emergency because it cannot stabilize sludge for a long time; drying stabilization is rarely used due to high investment and operation costs; aerobic digestion requires high power consumption, the treatment efficiency is reduced in cold seasons, and by-products are not practical value, only applicable to small sewage plants; anaerobic digestion is the most widely used, but there are safety hazards and high requirements for operation and management; composting is a biological treatment method, because it makes full use of useful components in sludge, turning waste into treasure, It is conducive to the establishment of a circular economy and has received widespread attention
[0004] At the same time, with the rise of industrial breeding technology, livestock and poultry manure in dairy farms, pig farms, chicken farms and other places has also become a major source of environmental pollution. Traditional composting technology is used to ferment slowly, produce a lot of odor, and the fermentation effect is poor.
Existing microbial agents are often used in the fermentation of livestock and poultry manure and sludge, but the activity of the strains is poor, the ecological environment of the flora is not strong enough, and the fermentation effect is unstable

Method used

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  • Strain for high-temperature composting of sludge and application thereof
  • Strain for high-temperature composting of sludge and application thereof
  • Strain for high-temperature composting of sludge and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The isolation and identification of embodiment 1 thermospheroid urea bacillus D1

[0036] 1) Sampling: Sampling 50g of the heap that has reached a high temperature of 50-70°C for more than 2 days, put it into a sterile Erlenmeyer flask, add 250ml of sterilized water, and shake for 30 minutes.

[0037] 2) Separation: In a sterile operating bench, the above-mentioned samples are coated on a flat plate by the extinction dilution method, put into a culture bag, and put into an incubator together.

[0038] Cultivation: place in a constant temperature incubator at 50-65°C for 12-48 hours;

[0039] Solid culture plate formula: according to the mass percentage of water, imported yeast powder 0.5-1%, sucrose 2%, industrial peptone 2%, sodium chloride 1%, agar 2.0-2.5%, distilled water, sterilized at 121 degrees Celsius for 30 minutes.

[0040]3) Purification: the cultured bacterial strains were separated by streaking on the above-mentioned solid culture plate until the pure cul...

Embodiment 2

[0049] The preparation of embodiment 2 compost inoculants

[0050] 1) Activation culture: activating and culturing the strain of Bacillus thermosphaeroidus D1 with the preservation number CGMCC No. 12329 at 60-80°C for 12-48 hours;

[0051] 2) Seed solution preparation: inoculate the activated strains into the culture solution, seal the bottle mouth, shake and cultivate at 65-80°C for 1-3 days, and the oscillation frequency is 160-200rpm;

[0052] 3) Preparation of fermented bacteria liquid: adding the seed liquid obtained in step 2) into the sterilized culture liquid at a volume ratio of 3%-10%, and the fermentation time is 48-72 hours. During the fermentation, the gas:liquid ratio of ventilation is 1:0.5 in 0-24 hours, 1:1 in 24-72 hours, the tank pressure of the fermenter is 0.08-0.1Mpa, and the stirring rate is 180-200rpm ;

[0053] 4) Preparation of compost inoculant: uniformly mix fermented bacteria liquid and sugar, add adsorption material, mix evenly with compost mat...

Embodiment 3

[0058] Application and effect of embodiment 3 compost inoculants of the present invention

[0059] 1. Sludge composting effect

[0060] The application effect of the compost inoculant prepared in Example 2 of the present invention will be described below with the composting treatment of municipal sludge. Take municipal sludge and straw powder at a weight ratio of 1:0.5 and mix them evenly, then add the compost inoculant prepared in Example 2 at 0.5% of the total material, and carry out stacking and fermentation, which is inoculation treatment. Another control was set up without inoculation of compost inoculants for the test. Among them, for conventional treatment, turn over every 5 days or when the temperature exceeds 75°C; for inoculation treatment, turn over every 4-6 days. Determine the maturity of the compost through the temperature change and oxygen consumption rate of the compost, and monitor the odor emission during the composting process.

[0061] The number of days...

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Abstract

The invention discloses a strain for high-temperature composting of sludge and the application thereof and belongs to the technical field of microbial fermentation. The strain D1 is Ureibacillus thermosphaericus with the preservation number of CGMCC No.12329. The highest growth temperature of the strain is 80 DEG C, temperature increases fast during fermentation of organic matter such as composted sludge, the duration of high temperature is long, quick multiplication can be achieved in a high-temperature composting environment, organic matter is degraded, composting process is promoted, and all indexes meet the composting discharge standard. The invention further provides a preparing method and application of a composting inoculant prepared from the strain D1. The composting inoculant is low in production cost, stable in quality, capable of being applied to high-temperature sludge composting, environment-friendly, free of pollution, excellent in performance and high in economic benefits and social benefits, and water content of a pile is not increased.

Description

technical field [0001] The invention belongs to the technical fields of microbial fermentation technology and environmental protection, and in particular relates to a bacterial strain and application for high-temperature composting of organic pollutants such as sludge and poultry manure. Background technique [0002] With the continuous improvement of my country's urbanization level, the construction of sewage treatment facilities has developed rapidly. By the end of 2014, more than 4,436 urban sewage treatment plants had been built in my country, and the urban sewage treatment capacity had reached 171 million tons per day. In order to achieve the national emission reduction target It has made great contributions to the improvement of water environment. However, the construction and operation of the sewage plant will produce a large amount of surplus sludge. Based on the sludge production rate of 0.8‰, the total annual sludge production in the country will soon exceed 136,000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C05G1/00C12R1/01
CPCC05B17/00C05F11/08C12N1/20C12N1/205C12R2001/01C05D9/00C05D9/02C05F5/002C05F11/00C05F17/00
Inventor 孙学忠
Owner 中泥(北京)环保科技有限公司
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