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1, 5-sorbitan determination kit, preparation method and application thereof

A technology of sorbitan and kits, which is applied in the measurement of color/spectral characteristics, analysis by making materials undergo chemical reactions, and material analysis by observing the impact on chemical indicators, etc., which can solve the problem of low accuracy of low-value samples , detection needs to be expensive, and the reaction blank increases, etc., to achieve the effects of prolonging the stability of enzyme activity, removing feedback inhibition, and simple operation

Inactive Publication Date: 2021-01-22
中拓生物有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] At present, there are a series of problems in the enzymatic kits of 1,5-sorbitol, which are not easy to stabilize various enzymes, low-value samples have low accuracy, low sensitivity, increased reaction blank, and expensive equipment for detection.

Method used

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  • 1, 5-sorbitan determination kit, preparation method and application thereof
  • 1, 5-sorbitan determination kit, preparation method and application thereof
  • 1, 5-sorbitan determination kit, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075]A kit for the determination of 1,5-sorbitol, characterized in that the kit contains reagent R1 and reagent R2;

[0076]Reagent R1 contains the following ingredients:

[0077]ADA buffer 120mmol / L

[0078]Adenosine triphosphate disodium salt 5 mmol / L

[0079]Hexokinase 30KU / L

[0080]6-phosphate glucose dehydrogenase 20 KU / L

[0081]Nicotinamide adenine dinucleotide (NAD+) 5 mmol / L

[0082]Phosphoenolpyruvate monopotassium salt 6 mmol / L

[0083]4-aminoantipyrine (4-AA) 5 mmol / L

[0084]Pyruvate kinase 10 KU / L

[0085]Peroxidase 10KU / L

[0086]Lactate dehydrogenase 10KU / L

[0087]Ascorbic acid oxidase 5KU / L

[0088]Magnesium chloride 4g / L

[0089]Potassium chloride 4g / L

[0090]Casein 2g / L

[0091]Glucomannan 5g / L

[0092]Sorbitol 1g / L

[0093]Pluronic F-68 1g / L

[0094]Sodium azide 1g / L

[0095]The reagent R2 includes the following components at the following concentrations:

[0096]EPPS buffer 80mmol / L

[0097]Pyranose Oxidase 50 KU / L

[0098]TOOS 0.8g / L

[0099]Pc300 1g / L

[0100]N-methyl-D-glucosamine 1g / L

[0101]The pH value of reagent R1 is 6.0; the...

Embodiment 2

[0104]A kit for the determination of 1,5-sorbitol, characterized in that the kit contains reagent R1 and reagent R2;

[0105]Reagent R1 contains the following ingredients:

[0106]ADA buffer 150mmol / L

[0107]Adenosine triphosphate disodium salt 6 mmol / L

[0108]Hexokinase 30KU / L

[0109]6-phosphate glucose dehydrogenase 40 KU / L

[0110]Nicotinamide adenine dinucleotide (NAD+) 5 mmol / L

[0111]Phosphoenolpyruvate monopotassium salt 6 mmol / L

[0112]4-aminoantipyrine (4-AA) 6 mmol / L

[0113]Pyruvate kinase 15 KU / L

[0114]Peroxidase 15KU / L

[0115]Lactate dehydrogenase 10KU / L

[0116]Ascorbic acid oxidase 5KU / L

[0117]Magnesium chloride 4g / L

[0118]Potassium chloride 4g / L

[0119]Casein 2g / L

[0120]Glucomannan 3g / L

[0121]Sorbitol 2g / L

[0122]Pluronic F-68 1g / L

[0123]Sodium azide 1g / L

[0124]The reagent R2 includes the following components at the following concentrations:

[0125]EPPS buffer 80mmol / L

[0126]Pyranose Oxidase 60KU / L

[0127]TOOS 0.8g / L

[0128]Pc300 1g / L

[0129]N-methyl-D-glucosamine 1g / L

[0130]The pH value of reagent R1 is 6.3; the ...

Embodiment 3

[0133]A kit for the determination of 1,5-sorbitol, characterized in that the kit contains reagent R1 and reagent R2;

[0134]Reagent R1 contains the following ingredients:

[0135]ADA buffer 150mmol / L

[0136]Adenosine triphosphate disodium salt 6 mmol / L

[0137]Hexokinase 50KU / L

[0138]6-phosphate glucose dehydrogenase 30 KU / L

[0139]Nicotinamide adenine dinucleotide (NAD+) 5 mmol / L

[0140]Phosphoenolpyruvate monopotassium salt 6 mmol / L

[0141]4-aminoantipyrine (4-AA) 5 mmol / L

[0142]Pyruvate kinase 10 KU / L

[0143]Peroxidase 18KU / L

[0144]Lactate dehydrogenase 10KU / L

[0145]Ascorbic acid oxidase 8KU / L

[0146]Magnesium chloride 4g / L

[0147]Potassium chloride 4g / L

[0148]Casein 2g / L

[0149]Glucomannan 3g / L

[0150]Sorbitol 3g / L

[0151]Pluronic F-68 1g / L

[0152]Sodium azide 1g / L

[0153]The reagent R2 includes the following components at the following concentrations:

[0154]EPPS buffer 100mmol / L

[0155]Pyranose Oxidase 50 KU / L

[0156]TOOS 0.8g / L

[0157]Pc300 1g / L

[0158]N-methyl-D-glucosamine 1g / L

[0159]The pH value of reagent R1 is 6.8; th...

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Abstract

The invention provides a 1, 5-sorbitan determination kit, a preparation method and an application thereof. The kit contains a reagent R1 and a reagent R2, wherein the reagent R1 contains the followingcomponents: a buffer solution, adenosine disodium triphosphate, hexokinase, glucose-6-phosphate dehydrogenase, nicotinamide adenine dinucleotide, phosphoenolpyruvate monopotassium salt, 4-amino antipyrine (4-AA), pyruvate kinase, peroxidase, lactic dehydrogenase, ascorbic acid oxidase, magnesium chloride, potassium chloride, an enzyme stabilizer and a preservative; and the reagent R2 is preparedfrom the following components in concentration: the buffer solution, pyranose oxidase, N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline sodium salt, a surfactant and the preservative. The kit is aliquid kit with strong stability, high accuracy, high sensitivity and a stable glucose elimination capability, can be suitable for a full-automatic biochemical analyzer, and is simple and convenient to operate.

Description

Technical field[0001]The invention relates to the technical field of biochemical reagent determination, in particular to a 1,5-sorbitan determination kit, and also relates to a preparation method and application of the 1,5-sorbitan determination kit.Background technique[0002]1,5-Anhydro-D-sorbitol, also known as 1,5-anhydro-D-glucoside, is a six-carbon monosaccharide with a pyran ring structure. 1,5-Sorbitan (1,5-AG) is formed by the deoxygenation of the first carbon (C1) of the pyranose ring structure. It belongs to the glucose homotype of polyhydric alcohol, and its content is in polyhydric alcohol. It is second only to glucose in carbohydrates. The structure of 1,5-AG is very similar to that of glucose, but the main difference between the two is that the hydroxyl group at the C1 position of glucose is replaced by hydrogen, and this substitution is exactly the chemical nature of 1,5-anhydro-D-sorbitol Stable foundation.[0003]In the early 1990s, Japan used 1,5-anhydro-D-sorbitol as...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31G01N35/00
CPCG01N21/31G01N21/78G01N35/00
Inventor 刘安娜
Owner 中拓生物有限公司
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