A method for producing cellulase by mixed fermentation of aspergillus oryzae and fusarium chlamydoides
A technology of Fusarium chlamydoides and cellulase, applied in the field of mixed fermentation of Aspergillus oryzae and Fusarium chlamydoides to produce cellulase
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[0017] The present invention will be further described below in conjunction with specific examples, but the protection scope of the present invention is not limited to the following examples.
[0018] (1) Strain storage:
[0019] Aspergillus oryzae HML366 and Fusarium chlamydogum HML278 were stored on PDA slant at 4°C;
[0020] (2) Method for purifying β-glucosidase BG HG2 from Aspergillus oryzae HML366
[0021] Include the following steps: S1. add the Aspergillus oryzae HML366 obtained by screening to 10 mL physiological saline to make spore liquid, take 10 7 The spores were transferred to bagasse solid medium for solid-state fermentation. Bagasse solid medium: 6 g bagasse, 4 g bran, 30 mL Mandels nutrient solution, cultured at 30 °C for 4 days; the culture was added with 200 mL sterile ddH 2 O, extract in a constant temperature water bath at 40°C for 1~3h, filter with four layers of gauze, centrifuge at 6000 r / min for 10min to obtain a crude enzyme solution, collect the su...
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