Novel process for preparing polysaccharide by high-valued comprehensive utilization of wheat bran
A technology for comprehensive utilization of wheat bran, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve the problems of prolonging the processing chain of agricultural and sideline products, low xylanase activity, and low yield of xylan problems, to achieve the effects of efficient utilization, easy scale production, and simple production process
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Embodiment 1
[0019] A new process for preparing polysaccharides from wheat bran with high-value comprehensive utilization is characterized in that it specifically includes the following steps:
[0020] (1) Pulverization: select bran without mildew and pulverize it through a 40-mesh sieve;
[0021] (2) Acid hydrolysis: Mix the pulverized bran and water at a mass ratio of 1:10, then pretreat with 0.1% citric acid solution for 1 hour, and control the acid hydrolysis temperature at 50-60°C;
[0022] (3) Ultrasonic-assisted enzymatic hydrolysis of cellulose: adjust the pH of the acid hydrolysis solution to 6.0-7.0, add cellulase with a weight ratio of 5% bran, control the temperature at 30-40°C, and perform enzymatic hydrolysis for 40 minutes under ultrasonic-assisted conditions. 300W, use a mixer to stir at a constant speed during the period;
[0023] (4) Ultrasonic-assisted secondary enzymatic hydrolysis: adjust the pH of the suspension to 8.0-9.0, add alkaline protease at a ratio of 2% by m...
Embodiment 2
[0031] A new process for preparing polysaccharides from wheat bran with high-value comprehensive utilization is characterized in that it specifically includes the following steps:
[0032] (1) Pulverization: select bran without mildew and pulverize it through a 40-mesh sieve;
[0033] (2) Acid hydrolysis: Mix the pulverized bran and water at a mass ratio of 1:8, then pretreat with 0.1% citric acid solution for 3 hours, and control the acid hydrolysis temperature at 50-60°C;
[0034] (3) Ultrasonic-assisted enzymatic hydrolysis of cellulose: adjust the pH of the acid hydrolysis solution to 6.0-7.0, add cellulase with a weight ratio of 3% bran, control the temperature at 30-40°C, and enzymatically hydrolyze for 60 minutes under ultrasonic-assisted conditions. 300W, use a mixer to stir at a constant speed during the period;
[0035] (4) Ultrasonic-assisted secondary enzymolysis: adjust the pH of the suspension to 8.0-9.0, add alkaline protease at a ratio of 1% by mass of bran, a...
Embodiment 3
[0043] A new process for preparing polysaccharides from wheat bran with high-value comprehensive utilization is characterized in that it specifically includes the following steps:
[0044] (1) Pulverization: select bran without mildew and pulverize it through a 40-mesh sieve;
[0045] (2) Acid hydrolysis: Mix the pulverized bran and water at a mass ratio of 1:9, then pretreat with 0.3% citric acid solution for 2 hours, and control the acid hydrolysis temperature at 50-60°C;
[0046] (3) Ultrasonic-assisted enzymatic hydrolysis of cellulose: adjust the pH of the acid hydrolysis solution to 6.0-7.0, add cellulase with a mass ratio of 4% bran, control the temperature at 30-40°C, and enzymolyze for 50 minutes under ultrasonic-assisted conditions. 300W, use a mixer to stir at a constant speed during the period;
[0047] (4) Ultrasonic-assisted secondary enzymolysis: adjust the pH of the suspension to 8.0-9.0, add alkaline protease at a ratio of 1.5% by mass of bran, and enzymolyze...
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