Method for producing cellulase by mixed fermentation of aspergillus oryzae and fusarium chlamydosporum

A technology of Fusarium chlamydoides and cellulase, applied in the field of mixed fermentation of Aspergillus oryzae and Fusarium chlamydoides to produce cellulase

Inactive Publication Date: 2017-12-12
HECHI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, there is no report on the synergistic fermentation of cellulase by Fusarium and Aspergillus oryzae

Method used

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  • Method for producing cellulase by mixed fermentation of aspergillus oryzae and fusarium chlamydosporum
  • Method for producing cellulase by mixed fermentation of aspergillus oryzae and fusarium chlamydosporum

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Embodiment Construction

[0017] The present invention will be further described below in conjunction with specific examples, but the protection scope of the present invention is not limited to the following examples.

[0018] (1) Strain storage:

[0019] Aspergillus oryzae HML366 and Fusarium chlamydogum HML278 were stored on PDA slant at 4°C;

[0020] (2) Method for purifying β-glucosidase BG HG2 from Aspergillus oryzae HML366

[0021] Include the following steps: S1. add the Aspergillus oryzae HML366 obtained by screening to 10 mL physiological saline to make spore liquid, take 10 7 The spores were transferred to bagasse solid medium for solid-state fermentation. Bagasse solid medium: 6 g bagasse, 4 g bran, 30 mL Mandels nutrient solution, cultured at 30°C for 4 days; the culture was added with 200 mL sterile ddH 2 O, extract in a constant temperature water bath at 40°C for 1~3h, filter with four layers of gauze, centrifuge at 6000 r / min for 10min to obtain a crude enzyme solution, collect the sup...

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Abstract

The invention discloses a method for producing cellulase by mixed fermentation of aspergillus oryzae and fusarium chlamydosporum. The method comprises the following steps: inoculating the fusarium chlamydosporum and the aspergillus oryzae on a bagasse solid culture medium and performing mixed culture fermentation, wherein the culture temperature is 20-50 DEG C, and pH value is 4.0-10.0; finishing fermentation culture after the maximum cellulose yield is reached. The method disclosed by the invention has the benefits that through the mixed culture of the aspergillus oryzae and the fusarium chlamydosporum, high-transglycoside activity belta-glucosidase produced by the aspergillus oryzae can synthesize gentiobiose, and the gentiobiose can stimulate the synthesis of the cellulase from the fusarium chlamydosporum; through the mixed culture of the aspergillus oryzae and the fusarium chlamydosporum, the composition of a cellulase system is optimized, so that the overall enzyme activity of the cellulase system can be significantly improved.

Description

technical field [0001] The invention relates to the technical field of cellulase production, in particular to a method for producing cellulase by mixed fermentation of Aspergillus oryzae and Fusarium chlamydoides. Background technique [0002] Cellulase (β-1,4-glucan-4-glucan hydrolase) is a general term for a group of enzymes that degrade cellulose to generate glucose. It is not a monomeric enzyme, but a synergistic multi-component The enzyme system is a complex enzyme, mainly composed of exo-β-glucanase, endo-β-glucanase, and β-glucosidase, as well as xylanase with high activity. β-glucosidase can hydrolyze cellobiose to relieve this feedback inhibition, improve the degradation efficiency of cellulase, and play a very key role in the degradation of cellulose. [0003] Trichoderma is the main industrial strain of cellulase, which can produce endoglucanase and exoglucanase with high activity, but has the defects of insufficient secretion of β-glucosidase and low enzyme acti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/42C12P19/14C12P19/02C12R1/69C12R1/645
CPCC12N9/2437C12N9/2445C12P19/02C12P19/14C12Y302/01004C12Y302/01021C12Y302/01091
Inventor 覃拥灵何海燕
Owner HECHI UNIV
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