52results about How to "Broad substrate spectrum" patented technology

The invention discloses a nitrile hydratase as well as an encoding gene and application thereof. The nitrile hydratase is prepared from an alpha subunit and a beta subunit, wherein an amino acid sequence of the alpha subunit is shown as SEQ ID NO.4, and an amino acid sequence of the beta subunit is shown as SEQ ID NO.5. According to the nitrile hydratase as well as the encoding gene and the application thereof, a nitrile hydratase gene is cloned from acid-producing Klebsiella spp KCTC 1686, and the nitrile hydratase with high expression level, high activity, wide substrate spectrum and chiral selectivity is successfully obtained after the gene is expressed.

The invention relates to a new microbe Bacillus fordii MH602 strain which can simultaneously produce hydantoinase and L-N-carbamyl hydrolase, the hydantoinase and L-N-carbamyl hydrolase produced by the strain, and an application for preparing serial optical pure L-amino acid through the strain. The hydantoinase and L-N-carbamyl hydrolase that are produced by Bacillus fordii MH602 strain can overcome the defect of worse activity and heat stability of the hydantoinase and L-N-carbamyl hydrolase in the L-amino acid preparation process by prior biologically inverting DL-5 substituted hydantoin or N-hydantoinamino acid, which has wide substrate spectrum, and can catalyze a plurality of substrates and have better substrate selectivity for aromatic hydantoin. For the natural amino acid and non-protein amino acid which are difficult to produce by a fermentation method or a chemosynthesis method, the preparation of L-amino acid by using the Bacillus fordii MH602 is simple and stable in operation, good in repeatability, which realizes stable and cheap preparation of L-amino acid and facilitates to scale production.

The invention discloses an enzyme-catalyzed C-terminal selective amidation and hydrazide modification method of polypeptide. The present invention provides a method for amidation and / or hydrazide modification of the C-terminal amino acid of a polypeptide, which is to use a specific protein to perform amidation and / or hydrazide modification of the C-terminal amino acid of the polypeptide; the specific protein is SEQ The protein indicated by ID No.1. Using this method, the amidation and / or hydrazide modification of the C-terminal amino acid of the polypeptide can be realized, and it is an aqueous phase reaction with mild conditions, and the C-terminus is selectively modified without affecting other modification groups on the side chain, and the side chain does not need to be protected , wide substrate spectrum, no restrictions on the amino acid sequence of the substrate polypeptide, any amino acid except proline at the end of the modified polypeptide can be modified.