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An extreme thermophilic bacterium and its application in high temperature composting fermentation

A thermophilic and bacterial technology, which is applied in the field of microbial technology and environmental engineering, can solve the problems of difficulty in reaching higher fermentation temperature and shortage, and achieve the effects of accelerating putrefaction, saving time and cost, and broad substrate spectrum

Active Publication Date: 2021-03-23
HANGZHOU XIUCHUAN TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] It can be seen from the above that the current high-temperature composting technology is mainly limited by the lack of efficient extreme thermophilic functional microorganisms, which makes it difficult to achieve higher fermentation temperatures.

Method used

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  • An extreme thermophilic bacterium and its application in high temperature composting fermentation

Examples

Experimental program
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Effect test

Embodiment 1

[0056] Embodiment 1: Food waste composting treatment

[0057] Take 5 tons of food waste, cut and stir to make it into pieces with a diameter of about 3cm. Put the pieces into the fermentation device, add 3% quicklime, 0.5g esterase, 0.8g cellulase, 0.4g alkaline amylase, 0.5g alkaline protease, control the moisture content of the material at about 80%, and stir evenly Then stand still for 0.5h. Then put 1.5 tons of GW-2 bacterial agent into the fermenter (when making, the volume ratio of fermentation broth to dry wood chips is 1:0.9), stir evenly and start heating. After 0.5h, the temperature in the fermenter reaches 45°C. After maintaining the heating for 3 hours, the temperature rises to 60°C, the heating is stopped, and the high-temperature composting stage is entered at this time. After 20 hours, the temperature rose to 100°C and the humidity dropped to 75%. After 24 hours, the temperature in the fermenter began to decrease slowly, and dropped to 60°C after 44 hours. At...

Embodiment 2

[0058] Example 2: Farming solid waste composting

[0059] Take 10 tons of pig farm feces, put in 4.5% quicklime, stir evenly, control the moisture content of the material at 82%, put in 0.8g esterase, 1.3g cellulase, 0.3g alkaline amylase, 0.8g alkaline protease and continue Mix and stir, turn on the electric heating to raise the temperature to 45°C and maintain it for 1h. Put in 4 tons of GW-2 bacterial agent sawdust, (during production, the volume ratio of fermentation liquid to dry sawdust is 1:0.8), stir well, increase the electric heating power to raise the temperature to 45°C, and start high-temperature composting. After 4 hours of start-up, the temperature of the compost heap rose to 65°C, and gradually increased within 24 hours of start-up, and finally reached 85-100°C, and the humidity gradually decreased from the initial 80% to about 53%. Then the temperature dropped slowly, and the temperature dropped to 65°C within 48 hours of starting. At this time, 20% of the vo...

Embodiment 3

[0060] Example 3: Fertilizer composting of agricultural by-products

[0061] Take 5 tons of straw, add it to the fermentation device with cutting and stirring, and continuously cut and stir it into pieces of about 6cm, put in 5% quicklime, stir evenly, control the moisture content of the material at 85%, put in 0.7g esterase, 1.3g cellulase , 0.6g alkaline amylase, and 0.5g alkaline protease continued to mix and stir, and turned on the electric heating to raise the temperature to 45°C and maintain it for 2h. Put in 2.5 tons of GW-2 bacterial agent sawdust, (during production, the volume ratio of fermentation liquid to dry sawdust is 1:1), stir well, increase the electric heating power to raise the temperature to 45°C, and start high-temperature composting. After 4 hours of start-up, the temperature of the compost heap rose to 60°C, and gradually increased within 24 hours of start-up, and finally reached 85-100°C, and the humidity gradually decreased from the initial 87% to abo...

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Abstract

The invention relates to the field of microbial technology and environmental engineering, and discloses an extreme thermophile and its application in high-temperature composting fermentation. The extreme thermophile is named GW-2, and its preservation number is CGMCC 18012, and the classification of microorganisms is named for Chelativorans composti . The strain of the present invention can grow normally in the range of 40-105°C, pH 5-11, and salinity 0-7%, can utilize various carbon sources and nitrogen sources for growth, and has a very broad substrate spectrum. The strain of the present invention combined with the special high-temperature composting process of the present invention can be used for high-temperature aerobic composting treatment of various organic matter such as kitchen waste, agricultural product tailings, slaughterhouse waste, etc., so that the organic matter is completely humified, dehydrated and dried, and loses weight by 70- 92%, finally forming a high-quality organic fertilizer.

Description

technical field [0001] The invention relates to the fields of microbial technology and environmental engineering, in particular to an extreme thermophilic bacterium and its application in high-temperature composting fermentation. Background technique [0002] Human activities produce solid waste containing a large amount of organic matter, such as kitchens. If not treated, it will cause serious pollution to the environment. The high-temperature composting technology that has gradually emerged in recent years can effectively stabilize these solid wastes. High-temperature composting is a process that uses microbial growth activities to decompose organic matter and convert it into humus under high temperature conditions. According to the different microorganisms used, it can be divided into Haoyang high-temperature composting and anaerobic high-temperature composting. At present, Haoyang high-temperature composting technology is widely used at home and abroad. Compared with an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C05F17/20C12R1/01
CPCC02F3/34C05F17/00C12N1/20C12N1/205C12R2001/01Y02W30/40
Inventor 王开彬张文武秦彦军王伟杨宇斯夏雨陈红玲
Owner HANGZHOU XIUCHUAN TECH CO LTD
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