A method for enzyme-catalyzed selective amidation and hydrazide modification of polypeptide C-terminus

Abstract

The invention discloses an enzyme-catalyzed polypeptide C-terminal selective amidation and hydrazidation modification method. The present invention provides a method for amidation and / or hydrazidation modification of the C-terminal amino acid of a polypeptide, which uses a specific protein to amidate and / or hydrazidate the C-terminal amino acid of the polypeptide; the specific protein is SEQ The protein shown in ID No.1. This method can be used to achieve amidation and / or hydrazidation modification of the C-terminal amino acid of the polypeptide, and it is an aqueous reaction with mild conditions. The C-terminal is selectively modified without affecting other modification groups on the side chain, and the side chain does not need to be protected. , with a wide substrate spectrum and no restriction on the amino acid sequence of the substrate polypeptide. Any amino acid at the terminal end of the modified polypeptide can be modified except proline.

Description

technical field [0001] The invention relates to the field of polypeptide chemistry, in particular to an enzyme-catalyzed C-terminal selective amidation and hydrazide modification method of polypeptide. Background technique [0002] The terminal functionalization of a polypeptide has a very important impact on its biochemical properties. Through the specific modification of the C-terminus, the in vivo metabolic half-life of the polypeptide can be prolonged, and the immunogenicity or toxic side effects can be reduced. In the study of the biochemical properties of peptides, researchers often need to add different functional groups (for example, fluorescent groups, synthetic polymers or immunolabels) to the peptide structure. In addition, efficient and accurate peptide C-terminal protection, deprotection and selective activation methods are also urgently needed technologies in industrial peptide synthesis and production. Therefore, the selective C-terminal modification method ...

AI Technical Summary

Problems solved by technology

[0003] Due to the complexity of the polypeptide structure, there are many chemical modification steps, low yield and great difficulty

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