223 results about "Humin" patented technology

The invention discloses a complex microbial inoculant for coupling straw decomposition and soil carbon sequestration and a preparation method thereof, the complex microbial inoculant can rapidly promote degradation of lignocellulose of straw in soil by compounding bacillus subtilis, bacillus licheniformis, pseudomonas aeruginosa and the like, provides a basic material energy source for activity of soil microorganisms, and has the advantages of simple preparation process and low cost. Meanwhile, corresponding microorganisms achieve long-term storage of organic carbon through humus stabilization and mineral substance combined carbon (MAOC) stabilization, the storage efficiency of the organic carbon is remarkably improved through nano Fe3O4, and under the synergistic effect of all strains in the complex microbial inoculant, the carbon loss of straw returning can be effectively reduced, the carbon immobilization capacity of soil is improved, and the soil quality is improved. The organic carbon content in the soil is increased.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus amyloliquefaciens with high H2O2 yield and application of the bacillus amyloliquefaciens. According to the bacillus amyloliquefaciens C-50-11, H2O2 can be continuously generated in the high-temperature period of compost, H2O2 free radicals can oxidize and decompose complex organic matter to promote humus condensation in a mediated mode, the content of humus in the compost in the high-temperature period is further effectively increased, and compared with the content of humus in uninoculated compost, the content of the humus after inoculation is increased by 20% or above. According to the method disclosed by the invention, chemical degradation is realized by combining an endogenous H2O2 strengthening effect of thermophilic bacteria and utilizing an oxidizing agent generated by organisms, so that the condition of a high-temperature period of compost is fully utilized, and a new method way is provided for increasing the content of humus in the compost.

The invention relates to a method for degrading nitrobenzene pollutants by using a humus-synergistic enhanced Fenton system, which comprises the following steps: adding a peroxide oxidant, a Fe (III) catalyst and a water-soluble organic reducing agent with Fe (III) reducing capacity into soil containing nitrobenzene pollutants, uniformly mixing to form a Fenton system, adding humus, and uniformly mixing to form the nitrobenzene pollutants. Mixing and reacting to obtain the soil for degrading the nitrobenzene pollutants. Compared with the existing Fenton technology, the method has the advantages that the overall catalytic efficiency can be improved, the initial pH does not need to be adjusted, and the method is green, safe and environmentally friendly.

The invention relates to the technical field of ecological agriculture, and discloses a phytase fungicide fertilizer as well as a production method and application thereof. 1-10 parts of a phytase high expression strain; 1-8 parts of a phytase solution; 1.7 to 9 parts of an embedding material; 3-8 parts of pH buffer particles; 10-30 parts of a micro-carbon carrier; 0.02 to 0.10 part of an indoleacetic acid microcapsule; and 30-60 parts of humus powder. The invention further provides a production method of the phytase fungicide fertilizer. The method comprises the following steps: S1, performing liquid fermentation on a phytase high-expression strain; s2, mixing the thalli with an enzyme solution; s3, adding pH buffer particles into the embedded particles; s4, drying the granules in a spray drying manner; and S5, mixing the dried product with humus powder, and granulating. By constructing a microbial inoculum system containing a phytase high-expression strain, efficient activation and conversion of organic phosphorus resources are realized, and the release efficiency of the fertilizer to phosphorus resources is improved.

The invention discloses Aspergillus sp. DLF18 and application thereof, and relates to the technical field of microorganisms, and the preservation number of the Aspergillus sp. DLF18 is CGMCC (China General Microbiological Culture Collection Center) NO.41737. The strain can effectively utilize rice straws and / or bagasse to produce humic acid, and the content of humic acid in each gram of fermentation supernatant after the rice straws are fermented is 16.5%. When the DLF18 fermentation product is applied to crops, the growth condition of the crops can be remarkably improved, and the humus content in soil is increased. Meanwhile, a metagenomics analysis result shows that compared with a control group, after the DLF18 is applied into the soil, the species beta diversity is remarkably changed, and the functional gene abundance and the inter-group difference are remarkably changed. Therefore, the DLF18 has a wide application prospect in the aspects of agricultural straw utilization and soil remediation.

The invention relates to the technical field of organic solid waste resourceful treatment, in particular to a method for enhancing degradation of biodegradable plastic compost based on white-rot fungi and manganese modified charcoal. According to the method, manganese modified charcoal and white-rot fungi are subjected to synergistic treatment, and the efficient method for intensively degrading the biodegradable plastic through aerobic composting is constructed. The method effectively overcomes the technical bottlenecks of slow degradation starting, long degradation period and easy generation of micro-plastic residues of the biodegradable plastic in traditional compost, and realizes rapid and thorough degradation of the biodegradable plastic. Besides, the method also promotes the conversion of humus precursor substances in the compost material and accelerates and stabilizes the synthesis of humus, so that the decomposition degree of the compost product is remarkably improved, and the quality and environmental safety of the final compost product are synchronously improved while the biodegradable plastic is efficiently degraded. And an efficient and reliable solution is provided for recycling of organic solid wastes and degradation of biodegradable plastic compost.

The invention discloses Penicillium chrysogenum DLS189 and application thereof, and relates to the technical field of microorganisms, and the preservation number of the Penicillium chrysogenum DLS189 is CGMCC (China General Microbiological Culture Collection Center) NO.41739. The invention further discloses a preparation method of the Penicillium chrysogenum DLS189. The strain can effectively utilize rice straws and / or bagasse to produce humic acid, and the content of humic acid in each gram of fermentation supernatant after the rice straws are fermented is 16.84%. When the DLS189 fermentation product is applied to crops, the growth condition of the crops can be remarkably improved, and the humus content in soil is increased. Meanwhile, a metagenomics analysis result shows that compared with a control group, after the DLS189 is applied into the soil, the species beta diversity is remarkably changed, and the functional gene abundance and the inter-group difference are remarkably changed. Therefore, the DLS189 has a wide application prospect in the aspects of agricultural straw utilization and soil remediation.

The invention relates to the technical field of sewage treatment, and particularly discloses a high-suspended-matter sewage treatment agent for an oil extraction plant. The invention discloses a high-suspended-matter sewage treatment agent for an oil extraction plant. The high-suspended-matter sewage treatment agent comprises 40-60 parts of modified bentonite; 5-15 parts of ferrous sulfate; 5-15 parts of aluminum ferric sulfate; 1 to 5 parts of ethylenediamine tetraacetic acid; 10 to 20 parts of an active biochar material; 3 to 8 parts of chitosan; 5 to 10 parts of a flocculating agent; the modified bentonite is obtained by modifying bentonite through laccase and nano zero-valent iron; the active biochar material is obtained by taking waste biomass as a raw material and carrying out semi-carbonization and activation treatment. The sewage treatment agent disclosed by the invention can be used for purifying high-suspended-matter sewage generated by oil extraction plants and oil wells, has high removal efficiency on suspended matters, and can effectively remove organic humus, so that the content of the suspended matters in the treated sewage reaches the standard.

A strengthening method for aerobic co-compost of kitchen waste anaerobic biogas residues and hermetia illucens feces belongs to the technical field of organic solid waste treatment, and the specific scheme comprises the following steps: step 1, uniformly mixing kitchen waste biogas residues, hermetia illucens feces and auxiliary materials to obtain a mixed material I; step 2, adding tea leaf residues and iron oxide into the mixed material I, and uniformly mixing to obtain a mixed material II, and step 3, carrying out aerobic composting on the mixed material II until the temperature rise period, the high-temperature period, the cooling period and the decomposition period of a compost body are normally completed. On the basis of a biogas residue-fecula aerobic co-composting system, by adding the tea leaf residues and the iron oxide, the content of humus precursor substances is increased, and conversion of the precursor substances to humus is promoted, so that formation of humic acid is facilitated, the fertilizer efficiency and application potential of a composting product are improved, and meanwhile nitrogen loss in the composting process is reduced.

The invention belongs to the technical field of artificial wetland regenerated water treatment, and particularly relates to an enhanced removal method for nitrate nitrogen and organic matters in an artificial wetland regenerated water source. According to the method, glucose, manganese ions and copper ions are added into a regenerated water source, heterotrophic bacteria are enriched in a constructed wetland packed bed, metal ions are loaded in the constructed wetland packed bed, extracellular active enzymes are generated through stress reaction of the heterotrophic bacteria to the metal ions, and then the enzymolysis process of humus organic matters difficult to biodegrade is achieved. The biodegradability of the recycled water is improved, and finally, the removal process of nitrate nitrogen and organic matters is enhanced.

The invention discloses a method for preparing porous carbon by using humin, and belongs to the field of carbon materials. The concentrated sulfuric acid is used for acidizing the humin under the conditions of high temperature and high pressure, so that the humin which is approximately spherical can be obtained, and the humin is pre-carbonized to form a relatively stable spherical structure. According to the invention, by controlling the temperature, on the premise of co-activation of carbon dioxide and water vapor and control of the gas flow rate, the porous carbon spheres with smooth surfaces can be obtained, and the yield can be improved at the same time. The method is simple to operate, the obtained porous carbon can well meet the preparation requirement of the silicon-carbon negative electrode, and the effect of turning waste into wealth is achieved.

The invention belongs to the technical field of compost, and particularly relates to a compost maturity evaluation method and application. The compost maturity evaluation method comprises the following steps: recording a temperature curve in a compost process, and taking the time when the compost temperature appears on a platform or the whole compost period as a calculation point; calculating the total heat generated from the beginning of composting to the calculation point, and recording the total heat as sigma Q; respectively measuring a seed germination rate index, humus concentration and organic matter concentration of compost before composting and at a calculation point; the difference value of the seed germination rate indexes, the difference value of the humus concentration and the difference value of the organic matter concentration are calculated and recorded as delta GI, delta HA and delta OM respectively; and calculating an organic fertilizer effect F and a humic index f, and evaluating the composting degree of the compost according to the f value. According to the method, each evaluation index is comprehensively considered, and the composting effects of different composting raw materials, different composting parameters and the like can be compared through the f value, so that the composting effects of different composts can be uniformly evaluated.

The invention relates to the technical field of waste recycling, and particularly discloses a method for preparing humus by subcritical hydrolysis of waste mushroom sticks, which comprises the following steps: adding mushroom stick powder into alkali liquor or acid liquor, adding H2O2 and FeSO4, adding ethylenediamine tetraacetic acid under an alkaline condition, and uniformly stirring to form a mixture; transferring the mixture into a high-pressure reaction kettle, reacting for a period of time at normal temperature, and then carrying out subcritical hydrothermal reaction to obtain a product 1; continuing to decay the product 1 and then carrying out suction filtration and solid-liquid separation to obtain a humus-containing liquid product. According to the method, the waste mushroom sticks are subjected to subcritical hydrolysis treatment to prepare the humus, the hydrolysis efficiency of the mushroom sticks is remarkably improved, and the hydrolysis rate reaches 80% or above. In addition, the temperature of the whole hydrothermal reaction is lower than that of a traditional pyrolysis or chemical oxidation process, energy consumption is greatly reduced, meanwhile, damage of high-temperature treatment to the humic acid structure is avoided, and the functionality of the product is effectively protected.

The invention discloses an ecological slope protection structure of a loess slope and a construction method. The slope protection structure comprises a deep solidification layer, an iron gauze, a solidification soil layer, a reinforcement-moisturizing layer and a seed layer. The deep curing layer is formed by mixing a consolid curing agent water aqua C444 and loess; the iron gauze covers the surface of the deep curing layer and is anchored by reinforcing steel bars; the solidified soil layer is formed by mixing a consolid curing agent and loess; the reinforced-moisturizing layer is formed by mixing a consolid curing agent, humus, a water-retaining agent, an organic fertilizer, basalt fibers and loess; the seed layer is formed by mixing seeds, a soil stabilizer, an organic fertilizer, loess and water. The soil stability can be better enhanced through the soil solidification and grass planting ecological slope protection technology, soil erosion is reduced, rain wash is slowed down, and therefore the risk of loess slope surface damage is reduced.

The present invention discloses a composite microbial agent for coupling straw decomposition and soil carbon sequestration and a preparation method thereof. The composite microbial agent of the present invention can rapidly promote the degradation of lignocellulose of straw in soil by compounding Bacillus subtilis, Bacillus licheniformis, Pseudomonas aeruginosa, etc., providing a basic material energy source for the activities of soil microorganisms. At the same time, the corresponding microorganisms achieve long-term sequestration of organic carbon through humus stabilization and mineral-associated organic carbon (MAOC) stabilization, and significantly enhance the efficiency of organic carbon sequestration through nano-Fe3O4. Under the synergistic action of various strains in this composite microbial agent, it can effectively reduce the carbon loss during straw returning to the field, improve the soil carbon sequestration capacity, and increase the organic carbon content in the soil.

The invention discloses a composite high-temperature bacterial agent and a method for promoting humification of compost by using the composite high-temperature bacterial agent. The composite high-temperature bacterial agent comprises at least one or two of bacillus thermotolans and Novibacillus thermophilus. The complex microbial inoculant disclosed by the invention is a thermophilic complex microbial inoculant, can directly utilize nutrient substances in organic solid wastes such as municipal sludge, livestock and poultry manure, agricultural residues, kitchen wastes and the like to grow and breed, and meanwhile, humus in compost is reduced; hydroxyl free radicals are continuously generated along with the change of anaerobic / aerobic alternating conditions of the compost, and the humification process of the compost is promoted through the free radicals.

The invention relates to the technical field of organic solid waste composting, in particular to an organic solid waste composting process utilizing electric field-microorganism synergistic fermentation. The conventional composting process for organic solid wastes is low in composting efficiency, and the content of humus in the obtained organic fertilizer is difficult to reach 140 g / kg or above. In order to solve the technical problems, the invention provides the organic solid waste composting process by utilizing electric field-microorganism synergistic fermentation, various organic solid wastes are mainly adopted for co-fermentation, the reasonable carbon-nitrogen ratio of compost is ensured, fermentation composting is performed under the synergistic effect of the electric field and microorganisms, the activity and metabolic rate of the microorganisms are promoted by the electric field, and the organic solid waste composting effect is improved. The method promotes the oxygenolysis and humus of organic matters, facilitates the generation of more humus in a short time, improves the composting efficiency, and guarantees the quality of the obtained organic fertilizer.

The invention discloses a carbon enzyme polypeptide water-soluble fertilizer and a preparation method thereof, and the water-soluble fertilizer is prepared from the following raw material components in parts by weight: 160-220 parts of urea crystal, 45-60 parts of polymeric carbon enzyme, 40-60 parts of potassium carbonate, 10-30 parts of polypeptide amino acid, 50-70 parts of pine pollen, 10-40 parts of trace elements, 30-60 parts of charcoal, 60-80 parts of modified ground phosphate rock, 2-6 parts of coenzyme and 30-80 parts of chitosan-humic acid slow-release copper fertilizer. According to the fertilizer synergist disclosed by the invention, the peat soil is added, so that the content of humus is greatly increased, the fertilizer synergist has a very good promoting effect on improving the soil fertility and improving the physical and chemical properties of soil after being applied, and meanwhile, the addition of various beneficial microbial agents can assist plant root systems to absorb the fertilizer, can fully release fertilizer nutrients, and improves the use effect of the fertilizer; therefore, growth of plant roots is promoted, and crop yield is increased.

The invention discloses a device for recycling crushed garden waste, and relates to the technical field of garden waste reutilization, the device comprises a device shell, the bottom of the device shell is fixedly communicated with a discharge pipe, and the top of the device shell is rotatably connected with a rotating main shaft in a penetrating manner; a rotating circular plate is fixedly connected to the end, located in the device shell, of the rotating main shaft, an auxiliary assembly used for improving the quality of compost products is arranged at the bottom of the rotating circular plate, and a third gear and a fourth gear are used in cooperation, so that a spiral column rotates in compost raw materials; compost raw materials at the bottom in the device shell are conveyed out of the raw materials through spiral rotation of the spiral column, so that the raw materials in compost are in contact with external air, necessary oxygen is obtained, organic substances are favorably converted into stable humus, the decomposition effect is more efficient, the composting period is favorably shortened, the production efficiency is improved, and the production cost is reduced. In the rotating process of the spiral column, moisture and heat in the compost raw materials are released.

The invention discloses a method for enhancing release-conversion of phosphorus in anaerobic fermentation of excess sludge by humus. In order to relieve the current situation of phosphorus resource shortage and depletion at present, the invention aims to improve the residual sludge fermentation phosphorus release performance by adding humus exogenous to the anaerobic fermentation process. The method comprises the following steps: 1, pretreating the excess sludge with low-temperature heat and acid to strengthen the disintegration and lysing efficiency of extracellular polymeric substances of the excess sludge, so as to provide sufficient substrates for subsequent anaerobic fermentation acid production and improve the release efficiency of phosphorus; 2, the pretreated sludge is subjected to anaerobic fermentation, humus is added to improve the solubility of Fe-P (Fe < 3 + > is reduced into Fe < 2 + >), and the humus and metal ions are competitively complexed to improve the release efficiency of soluble phosphorus. Compared with the method for reinforcing the release-conversion of the phosphorus in the excess sludge by the humus, the method has the advantages of low cost, no secondary pollution risk and the like, can achieve an ideal phosphorus release-conversion effect, and has important significance for realizing the recovery and recycling of phosphorus resources in the sludge.

The application discloses a kind of aquaculture tail water solubility organic matter characteristic analysis method and system, specifically related to water quality monitoring and analysis technical field, for solving the problem that existing solubility organic matter characterization method is difficult to effectively associate and predict its actual degradation behavior in biological treatment process.Synchronously obtain the three-dimensional fluorescence spectrum data of the water sample to be measured and the liquid chromatography-organic carbon detection combined analysis data, obtain the hydrophilic-hydrophobic component ratio and the proportion of tyrosine-like and humus-like fluorescence components from the analysis, and then judge whether there is significant humus-like component.According to the judgment result, selectively start the deep analysis of the molecular aggregation state of humus-like component, and comprehensively consider the related proportion parameters and aggregation state information, finally realize the evaluation of the biological degradation potential trend of solubility organic matter, and provide key basis for the stability warning of biological treatment system.

The invention discloses a preparation method and application of a wormcast granular bacterial fertilizer, and the wormcast granular bacterial fertilizer is prepared by adopting a base material ratio of wormcast to humus of 7: 3 through mechanical granulation, 80 DEG C constant temperature drying, particle cooling and functional bacterial agent spraying. The product has a remarkable application effect on various crops such as corn (grain corn, fresh corn and silage corn), hot peppers, tomatoes, traditional Chinese medicinal materials (radix glehniae, radix saposhnikoviae and radix achyranthis bidentatae) and the like, can give full play to the synergistic interaction effect of organic matrixes and functional microorganisms, and is stable in fertilizer efficiency, convenient to apply and suitable for industrial continuous production and large-area popularization and application.

The present invention discloses a method for determining the landfill age of stockpiled waste based on humus composition, which relates to the technical field of environmental protection. The method for determining the landfill age of stockpiled waste based on humus composition disclosed by the present invention includes the following steps: drilling and sampling the stockpiled waste; performing pre-treatments such as air-drying, screening, and impurity removal on the samples; measuring the humus composition and its content to obtain the carbon content of humic acid; measuring the three-dimensional fluorescence of the leaching solution of humic acid extracted from humus soil and performing parallel factor analysis to obtain the total fluorescence intensity of humic acid-like substances therein; fitting the unit fluorescence index F<subgt;u< / subgt; with the landfill age in the form of an exponential function; and then performing statistical analysis on the unit fluorescence index F<subgt;u< / subgt> and the landfill age. The method for determining the landfill age of stockpiled waste based on humus composition and its three-dimensional fluorescence intensity provided by the present invention is fast and simple, and can intuitively determine the stabilization degree and landfill age of the stockpiled waste in the landfill.

The invention is applicable to the technical field of straw treatment, and provides an automatic-distributing double-chamber straw compressing and smashing device which comprises a base, a conveying mechanism, a pressing mechanism, a distributing mechanism, a double-chamber smashing mechanism and a one-driving-two driving mechanism, and the double-chamber smashing mechanism comprises a first smashing cavity, a second smashing cavity and a smashing cutter; the material conveying mechanism is used for conveying straw raw materials to the material pressing mechanism; the pressing mechanism is used for compacting the straws; the material distributing mechanism is used for uniformly distributing straw blocks to the crushing cavity I and the crushing cavity II; the double-chamber crushing mechanism is used for synchronously crushing straw blocks in the crushing cavity I and the crushing cavity II; and the one-driving-two driving mechanism is used for synchronously driving the two crushing cutters. The straw treatment efficiency is remarkably improved, the smashing effect is optimized, it is ensured that smashed straw particles are fine and uniform, humus production is accelerated, equipment loss and energy consumption are reduced, the maintenance cost is reduced, and the straw smashing device is particularly suitable for large-scale straw resource utilization and artificial humus production.

This invention relates to DNA extraction methods from soil and sediments with high humic substances content. The method focuses on the soil preparation phase; removing salts and cations with two different Tris-EDTA (TE) buffers, followed by precipitating humic substances with aluminum hydroxide solution and washing again with TE and cell lysis phase; urea is used as denaturing agents for the cell lysis phase and protein degradation phase while preventing the rediffusion of humic substances with CTAB. The purification can be performed following both the classic phenol chloroform method and using commercial DNA purification columns. This method is very accessible with operation time varying from 1h40 min to 2h30 min. The invention involves the use of TE and aluminum hydroxide solutions for soil preparation, and urea and CTAB as denaturing agents during cell lysis, in the specified concentrations, order, and incubation time, to prevent contamination of final DNA with humic substances

The present invention belongs to the field of microbial technology and specifically relates to a composite microbial agent for promoting lignocellulose degradation and humus production during mulberry twig and cow dung composting, and its application. The composite microbial agent comprises a mixture of strains B1, F1, and F2, wherein the ratio of effective viable bacteria among strains B1, F1, and F2 is 1:1:1. The deposit number of strain B1 is GDMCC No. 65049; the deposit number of strain F1 is GDMCC No. 65050; and the deposit number of strain F2 is GDMCC No. 65051. The Bacillus cereus B1, Phanerochaete chrysosporium F1, and Sphaerotheca fusca F2 in the composite microbial agent are all effective fertilizer compost-promoting microorganisms. These three microorganisms can synergize with each other during aerobic composting, fully achieving the conversion and degradation of raw materials and producing high-quality organic fertilizer. Furthermore, the composite microbial agent can promote lignocellulose degradation and humus production during composting.

The present application relates to the field of microbiological technology, and specifically provides a compound microbial flora preparation for improving crop straw compost humification and organic carbon fixation and application thereof. The compound microbial flora is composed of saccharophagus degradans CGMCC 1.10397, paenibacillus popilliae CGMCC 1.12900 and bacillus subtilis CGMCC 1.6757. Seed liquids of the above three strains are mixed and cultured in a volume ratio of 1:1:1 to obtain a compound microbial suspension, which is applied to the aerobic composting process of crop straw, can significantly improve the degradation efficiency of lignocellulose, promote the formation of humus and the stable fixation of organic carbon, thereby improving the compost quality, reducing the nutrient loss and greenhouse gas emission. The present application is suitable for the resource treatment of cotton straw, vegetable straw and other agricultural organic wastes.

The present disclosure provides granular fertilizer compositions and methods of manufacturing thereof. The method for manufacturing the granular fertilizer comprises a declining weight measurement process, a mixing process, a compaction process, and a sorting and cleaning process to produce the granular fertilizer. The granular fertilizer composition may be comprised of urea, metabasalt, and a humic substance. The granular fertilizer composition may alternatively comprise metabasalt, biochar, and a humic substance. The present disclosure also provides a compound fertilizer composition comprising metabasalt at a preferred percentage by weight.

This invention discloses a bio-composite matrix for solid waste and yellow sand systems, its preparation method, and its application. Belonging to the technical field of bio-composite organic matrices, this invention utilizes the significant complementarity of fly ash, drum slag, and biological organic matter in terms of physical structure, chemical composition, and biological activity. Through compounding, an ecological restoration matrix with aeration, water retention, and nutrient supply capabilities can be constructed. Fly ash improves the matrix's pore distribution, enhancing permeability and water retention capacity. Simultaneously, its rich content of minerals such as Si, Al, and Ca can regulate the matrix pH and provide nutrients to a certain extent. Drum slag acts as a skeletal support, improving the composite matrix's mechanical strength and compressive strength, preventing matrix compaction and sedimentation. Biological organic matter, rich in organic matter, humus, and residual mycelium, possesses excellent water retention and slow-release nutrient capacity, significantly enhancing matrix fertility and microbial activity, providing carbon sources and nutritional support for plant root growth.