162 results about "Humin" patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus amyloliquefaciens with high H2O2 yield and application of the bacillus amyloliquefaciens. According to the bacillus amyloliquefaciens C-50-11, H2O2 can be continuously generated in the high-temperature period of compost, H2O2 free radicals can oxidize and decompose complex organic matter to promote humus condensation in a mediated mode, the content of humus in the compost in the high-temperature period is further effectively increased, and compared with the content of humus in uninoculated compost, the content of the humus after inoculation is increased by 20% or above. According to the method disclosed by the invention, chemical degradation is realized by combining an endogenous H2O2 strengthening effect of thermophilic bacteria and utilizing an oxidizing agent generated by organisms, so that the condition of a high-temperature period of compost is fully utilized, and a new method way is provided for increasing the content of humus in the compost.

The invention discloses Aspergillus sp. DLF18 and application thereof, and relates to the technical field of microorganisms, and the preservation number of the Aspergillus sp. DLF18 is CGMCC (China General Microbiological Culture Collection Center) NO.41737. The strain can effectively utilize rice straws and / or bagasse to produce humic acid, and the content of humic acid in each gram of fermentation supernatant after the rice straws are fermented is 16.5%. When the DLF18 fermentation product is applied to crops, the growth condition of the crops can be remarkably improved, and the humus content in soil is increased. Meanwhile, a metagenomics analysis result shows that compared with a control group, after the DLF18 is applied into the soil, the species beta diversity is remarkably changed, and the functional gene abundance and the inter-group difference are remarkably changed. Therefore, the DLF18 has a wide application prospect in the aspects of agricultural straw utilization and soil remediation.

The invention relates to the technical field of organic solid waste resourceful treatment, in particular to a method for enhancing degradation of biodegradable plastic compost based on white-rot fungi and manganese modified charcoal. According to the method, manganese modified charcoal and white-rot fungi are subjected to synergistic treatment, and the efficient method for intensively degrading the biodegradable plastic through aerobic composting is constructed. The method effectively overcomes the technical bottlenecks of slow degradation starting, long degradation period and easy generation of micro-plastic residues of the biodegradable plastic in traditional compost, and realizes rapid and thorough degradation of the biodegradable plastic. Besides, the method also promotes the conversion of humus precursor substances in the compost material and accelerates and stabilizes the synthesis of humus, so that the decomposition degree of the compost product is remarkably improved, and the quality and environmental safety of the final compost product are synchronously improved while the biodegradable plastic is efficiently degraded. And an efficient and reliable solution is provided for recycling of organic solid wastes and degradation of biodegradable plastic compost.

The invention discloses Penicillium chrysogenum DLS189 and application thereof, and relates to the technical field of microorganisms, and the preservation number of the Penicillium chrysogenum DLS189 is CGMCC (China General Microbiological Culture Collection Center) NO.41739. The invention further discloses a preparation method of the Penicillium chrysogenum DLS189. The strain can effectively utilize rice straws and / or bagasse to produce humic acid, and the content of humic acid in each gram of fermentation supernatant after the rice straws are fermented is 16.84%. When the DLS189 fermentation product is applied to crops, the growth condition of the crops can be remarkably improved, and the humus content in soil is increased. Meanwhile, a metagenomics analysis result shows that compared with a control group, after the DLS189 is applied into the soil, the species beta diversity is remarkably changed, and the functional gene abundance and the inter-group difference are remarkably changed. Therefore, the DLS189 has a wide application prospect in the aspects of agricultural straw utilization and soil remediation.

The invention discloses a composite high-temperature bacterial agent and a method for promoting humification of compost by using the composite high-temperature bacterial agent. The composite high-temperature bacterial agent comprises at least one or two of bacillus thermotolans and Novibacillus thermophilus. The complex microbial inoculant disclosed by the invention is a thermophilic complex microbial inoculant, can directly utilize nutrient substances in organic solid wastes such as municipal sludge, livestock and poultry manure, agricultural residues, kitchen wastes and the like to grow and breed, and meanwhile, humus in compost is reduced; hydroxyl free radicals are continuously generated along with the change of anaerobic / aerobic alternating conditions of the compost, and the humification process of the compost is promoted through the free radicals.

The invention discloses a carbon enzyme polypeptide water-soluble fertilizer and a preparation method thereof, and the water-soluble fertilizer is prepared from the following raw material components in parts by weight: 160-220 parts of urea crystal, 45-60 parts of polymeric carbon enzyme, 40-60 parts of potassium carbonate, 10-30 parts of polypeptide amino acid, 50-70 parts of pine pollen, 10-40 parts of trace elements, 30-60 parts of charcoal, 60-80 parts of modified ground phosphate rock, 2-6 parts of coenzyme and 30-80 parts of chitosan-humic acid slow-release copper fertilizer. According to the fertilizer synergist disclosed by the invention, the peat soil is added, so that the content of humus is greatly increased, the fertilizer synergist has a very good promoting effect on improving the soil fertility and improving the physical and chemical properties of soil after being applied, and meanwhile, the addition of various beneficial microbial agents can assist plant root systems to absorb the fertilizer, can fully release fertilizer nutrients, and improves the use effect of the fertilizer; therefore, growth of plant roots is promoted, and crop yield is increased.

The application discloses a kind of aquaculture tail water solubility organic matter characteristic analysis method and system, specifically related to water quality monitoring and analysis technical field, for solving the problem that existing solubility organic matter characterization method is difficult to effectively associate and predict its actual degradation behavior in biological treatment process.Synchronously obtain the three-dimensional fluorescence spectrum data of the water sample to be measured and the liquid chromatography-organic carbon detection combined analysis data, obtain the hydrophilic-hydrophobic component ratio and the proportion of tyrosine-like and humus-like fluorescence components from the analysis, and then judge whether there is significant humus-like component.According to the judgment result, selectively start the deep analysis of the molecular aggregation state of humus-like component, and comprehensively consider the related proportion parameters and aggregation state information, finally realize the evaluation of the biological degradation potential trend of solubility organic matter, and provide key basis for the stability warning of biological treatment system.

The invention discloses a preparation method and application of a wormcast granular bacterial fertilizer, and the wormcast granular bacterial fertilizer is prepared by adopting a base material ratio of wormcast to humus of 7: 3 through mechanical granulation, 80 DEG C constant temperature drying, particle cooling and functional bacterial agent spraying. The product has a remarkable application effect on various crops such as corn (grain corn, fresh corn and silage corn), hot peppers, tomatoes, traditional Chinese medicinal materials (radix glehniae, radix saposhnikoviae and radix achyranthis bidentatae) and the like, can give full play to the synergistic interaction effect of organic matrixes and functional microorganisms, and is stable in fertilizer efficiency, convenient to apply and suitable for industrial continuous production and large-area popularization and application.

This invention relates to DNA extraction methods from soil and sediments with high humic substances content. The method focuses on the soil preparation phase; removing salts and cations with two different Tris-EDTA (TE) buffers, followed by precipitating humic substances with aluminum hydroxide solution and washing again with TE and cell lysis phase; urea is used as denaturing agents for the cell lysis phase and protein degradation phase while preventing the rediffusion of humic substances with CTAB. The purification can be performed following both the classic phenol chloroform method and using commercial DNA purification columns. This method is very accessible with operation time varying from 1h40 min to 2h30 min. The invention involves the use of TE and aluminum hydroxide solutions for soil preparation, and urea and CTAB as denaturing agents during cell lysis, in the specified concentrations, order, and incubation time, to prevent contamination of final DNA with humic substances

The present application relates to the field of microbiological technology, and specifically provides a compound microbial flora preparation for improving crop straw compost humification and organic carbon fixation and application thereof. The compound microbial flora is composed of saccharophagus degradans CGMCC 1.10397, paenibacillus popilliae CGMCC 1.12900 and bacillus subtilis CGMCC 1.6757. Seed liquids of the above three strains are mixed and cultured in a volume ratio of 1:1:1 to obtain a compound microbial suspension, which is applied to the aerobic composting process of crop straw, can significantly improve the degradation efficiency of lignocellulose, promote the formation of humus and the stable fixation of organic carbon, thereby improving the compost quality, reducing the nutrient loss and greenhouse gas emission. The present application is suitable for the resource treatment of cotton straw, vegetable straw and other agricultural organic wastes.

This invention discloses a bio-composite matrix for solid waste and yellow sand systems, its preparation method, and its application. Belonging to the technical field of bio-composite organic matrices, this invention utilizes the significant complementarity of fly ash, drum slag, and biological organic matter in terms of physical structure, chemical composition, and biological activity. Through compounding, an ecological restoration matrix with aeration, water retention, and nutrient supply capabilities can be constructed. Fly ash improves the matrix's pore distribution, enhancing permeability and water retention capacity. Simultaneously, its rich content of minerals such as Si, Al, and Ca can regulate the matrix pH and provide nutrients to a certain extent. Drum slag acts as a skeletal support, improving the composite matrix's mechanical strength and compressive strength, preventing matrix compaction and sedimentation. Biological organic matter, rich in organic matter, humus, and residual mycelium, possesses excellent water retention and slow-release nutrient capacity, significantly enhancing matrix fertility and microbial activity, providing carbon sources and nutritional support for plant root growth.

This invention discloses a strain of Aspergillus sp., DLF18, and its applications, belonging to the field of microbial technology, with accession number CGMCC NO.41737. This strain can effectively utilize rice straw and / or sugarcane bagasse to produce humic acid, with a humic acid content of 16.5% per gram of fermentation supernatant after rice straw fermentation. Applying the fermentation product of DLF18 to crops can significantly improve crop growth and increase the humic content in the soil. Simultaneously, metagenomic analysis results show that, compared with the control group, the application of DLF18 to the soil significantly altered species β-diversity, functional gene abundance, and inter-group differences. These findings indicate that DLF18 has broad application prospects in agricultural straw utilization and soil remediation.

This invention discloses a soil chloride ion detection pretreatment device, its usage method, and a filter paper preparation method, relating to the field of laboratory equipment technology. The device includes a funnel placement rack with a double-compartment funnel inserted inside. Eight evenly distributed structural support ribs are fixedly installed on the inner wall of the double-compartment funnel. Folded composite filter paper and a nylon support mesh are placed sequentially on the structural support ribs. The upper layer of dilute nitric acid is used to wash away chloride from the activated carbon fiber layer, efficiently adsorbing colored substances such as humus and organic matter in the soil extract. This results in a clear and transparent filtrate, eliminating the interference of deep yellow and brown to the brick-red endpoint of the silver nitrate titration method. The endpoint is clearly distinguishable, and the chloride ion recovery rate is stabilized at 92.0%–105.0%. This solves the problem of the lack of a decolorization step in the silver nitrate titration method, leading to a blurred endpoint and a detection error of over 20% caused by high-chroma soil extract.

The present disclosure relates to biodegradable polymers, particularly to a method for converting biodegradable polymers into humus with a low release amount of CO2 and use thereof. A composite material system is formed from the biodegradable polymers and the materials that can slowly release nutrient nitrogen or nutrients nitrogen and phosphorus, in which the mass ratio of carbon to nitrogen in the composite material system is (1-35):1. The materials of the present disclosure can promote the biodegradable polymers to be converted into soil humus or compost humus, rather than to be converted into greenhouse gas CO2, which is to be emitted into the atmosphere.

The invention discloses a preparation method of nutrient soil based on geopolymer and titanium dioxide byproduct desulfurized gypsum as well as the prepared nutrient soil and application thereof, and relates to the technical field of industrial solid waste resource utilization and soil improvement. In order to solve the problems of land occupation, environment pollution, resource waste and the like in the traditional stockpiling treatment of the by-product desulfurized gypsum in the titanium dioxide industry, the titanium dioxide by-product desulfurized gypsum is used as a main raw material and is matched with a geopolymer base material, humus, a functional regulator and a porous filler; the composite nutrient soil with the functions of nutrient supply, structure improvement and pH regulation is prepared through the processes of raw material pretreatment, accurate proportioning, normal-temperature activation, screening and packaging and the like. Harmless treatment and recycling high-value utilization of the desulfurized gypsum are achieved, the prepared nutrient soil can improve different types of soil such as acidic soil, alkaline soil and viscous soil in a targeted mode, the air permeability, fertilizer retention and crop growth adaptability of the soil are remarkably improved, and the nutrient soil is suitable for industrial popularization.

The invention relates to the technical field of agricultural resources and environment, and discloses a preparation method and application of an in-situ activator for carbon sequestration, pollution reduction and yield increase of soil. The activator comprises the following raw materials in parts by mass: 5-15 parts of a modified mineral carrier, 10-15 parts of an organic slow-release matrix, 6-13 parts of a lactic acid bacteria crude extract, 5-15 parts of a fungus crude extract, 5-12 parts of a yeast crude extract, 10-20 parts of a microbial fermentation humus crude extract, 4-8 parts of a complexing agent, 5-10 parts of a protective agent, 3-8 parts of root-promoting signal molecules and 1-3 parts of quorum sensing molecules. According to the scheme, five units including a supporting framework, an active component, a complexing agent, a protective agent and a trace substance are jointly assembled to serve as a core of the activator, the activator is supported by a porous carrier, a compound enzyme-humus system and an oxygen-iron-sulfur electron shuttle system are colonized on the surface, nutrient substance aggregates are slowly released, and signal molecule induction is combined, so that the activity of the activator is improved. And soil carbon reservoir expansion, pollutant passivation and crop root development promotion are realized. According to the preparation, the soil carbon sequestration efficiency can be improved by 25% or above, the heavy metal activity can be reduced by 40-65%, and the average yield of crops can be increased by 15-20%.

The invention belongs to the technical field of soil improvement, and discloses a soil conditioner with an acidified soil improving function and a preparation method thereof. The conditioner is prepared from biomass charcoal, peat containing humic acid, crab shells, eggshells, clamshells, bamboo charcoal, zeolite powder, kieselguhr, biogas residues, potassium fulvate, calcium magnesium phosphate fertilizer, animal waste and a microbial agent according to the mass percent. The invention relates to the technical field of soil improvement. The modifier realizes a multi-dimensional synergistic effect through alkaline neutralization, humus buffering, slow-release fertilizer supply and microbial activation, can significantly improve the pH value of acidified rice field soil after one-time application, increases 0.6-1.0 unit, effectively increases the contents of soil organic matters, total nitrogen, available phosphorus and available potassium, improves the soil structure and microbial activity, and improves the soil quality. And the yield of rice is increased by about 10% or above. The method is suitable for soil improvement and green agricultural production of southern acidic rice fields.

The application discloses a method for preparing an electrochemical catalyst from solid humin, and first, solid humin is obtained, then pretreated humin powder, mixed metal salt and thiourea are added into a zirconium oxide or agate ball mill jar, then ethanol solution is added until the powder is just wet; subsequently, grinding balls are added, the jar cover is tightly covered, and the jar is placed in a planetary ball mill for ball milling; after the ball milling is completed, ethanol aqueous solution is used to wash out the slurry in the jar, the mixed solution is thinly spread in a container and transferred to an oven for drying; after drying, flaky particles are formed, which are ground into powder using a mortar and transferred to a porcelain boat and placed in a tube furnace for precalcination under a nitrogen flow; after the calcination is completed, the solid powder is collected, which is the humin derivative carbon supported transition metal sulfide electrocatalyst to be prepared; compared with a traditional carbon supported metal catalyst, the application has multiple advantages of high intrinsic activity, strong structural stability and excellent electronic adjustability in HMF oxidation, and provides an ideal material platform for efficient electrocatalytic conversion of high-purity biomass platform molecules.

The invention relates to the technical field of chemical preparation, and provides a method and system for preparing a humic acid compound fertilizer based on caprolactam byproduct APU oil, and the method comprises the following steps: carrying out a catalytic oxidation reaction on the caprolactam byproduct APU oil and an immobilized FeOCl catalyst under an aerobic condition to generate an active intermediate; carrying out condensation reaction on the active intermediate under an anoxic condition to generate a humic acid-like substance; and carrying out post-treatment on the humic acid-like substance to obtain the humic acid compound fertilizer. According to the scheme, the caprolactam byproduct APU oil is converted into the humic acid compound fertilizer which is high in organic matter content and good in soil improvement effect through catalytic oxidation and condensation reaction, resource utilization of industrial byproducts is achieved, and the production cost of the fertilizer is reduced. Moreover, the immobilized FeOCl catalyst is selected as the catalyst, on one hand, secondary pollution of a liquid-phase catalyst is avoided, and on the other hand, carbon and nitrogen elements in the caprolactam byproduct APU oil can be efficiently converted into humus and nutrients, so that the environmental protection property of the whole process is ensured from the source.

The utility model relates to the technical field of resource recycling, in particular to a straw humus fermentation bin for resource recycling, which comprises a fermentation tank, a first dosing pipe is coaxially and rotatably connected with a mixing unit; the mixing unit comprises a planetary transmission mechanism and a dosing mechanism; the dosing mechanism comprises a plurality of second dosing pipes of which one ends are communicated with the first dosing pipe; the other end of the second dosing pipe is connected with a third dosing pipe; a plurality of pesticide spraying holes are uniformly formed in the peripheral wall of each third pesticide adding pipe, and each pesticide spraying hole is communicated with an inner cavity of the corresponding third pesticide adding pipe; and each third dosing pipe is coaxially and fixedly provided with a plurality of rotating blades. According to the device, an additive can be uniformly sprayed to each layer of humus in the device, and meanwhile, the humus can be fully stirred. And by arranging the rotating blades in planetary motion, caked humus can be shredded, and the situation that humus caking affects absorption of the humus to the additive is reduced.

The invention discloses a compound microbial agent for efficient decomposition of mushroom dregs and application of the compound microbial agent in mushroom dreg decomposition, and belongs to the technical field of microorganisms. The compound microbial agent disclosed by the invention is prepared by compounding bacillus amyloliquefaciens (Bacillus amyloliquefaciens, CGMCC (China General Microbiological Culture Collection Center) No.36766), bacillus licheniformis (Bacillus licheniformis, CGMCC No.35039) and Argentina saltbox bacteria, and the compound microbial agent disclosed by the invention is prepared by compounding bacillus amyloliquefaciens, bacillus licheniformis, CGMCC No.36766, bacillus licheniformis and Argentina saltbox bacteria. The composite microbial agent can significantly accelerate the humification process of mushroom dregs, improve the duration time and stability of a high-temperature period of a pile body, promote conversion of refractory lignocellulose components, and finally obtain a high-quality decomposed substrate which is more developed in pore structure, higher in humus content, more stable in functional microbial community and capable of effectively adsorbing and passivating heavy metals. The invention also provides a method for preparing a mushroom dreg decomposition preparation by using the complex microbial inoculant.

The invention relates to the technical field of woody peat humus compositions, in particular to an iron-modified woody peat humus composition and application thereof in synchronous passivation of cadmium and arsenic in soil, and the iron-modified woody peat humus composition comprises the following raw materials: woody peat, a composite iron modifier, ammonium sulfate, monopotassium phosphate and microbial fermentation liquor. The composite iron modifier overcomes the problem that free iron is easy to precipitate in acid soil, and ensures stable release and efficient utilization of iron ions; secondly, iron ions can be subjected to complex reaction with carboxyl, phenolic hydroxyl and other functional groups in humic acid to form a stable organic-inorganic compound; zeolite can remarkably improve the cadmium adsorption capacity and enhance the dispersity of iron active components through ion exchange, and cadmium and arsenic are synchronously and efficiently passivated.

The utility model relates to the technical field of organic fertilizer processing, in particular to mixing and stirring equipment for compound organic fertilizer ingredients, which comprises a tank body, a shaft rod is fixed on the front end surface of the turntable close to the edge; a first stirring shaft; the outer wall of the first stirring shaft is in coaxial key connection with a plurality of first stirring blades; the first stirring blades and the second stirring blades rotate synchronously and are distributed in a staggered mode, materials can be stirred from multiple angles, and the shearing force on the materials is increased. The first stirring shaft and the first stirring blades can do reciprocating lifting motion in the rotating process, and the multi-dimensional stirring motion mode expands the stirring range and avoids stirring dead angles. The humus and the peat soil are combined to effectively break the agglomeration phenomenon of the materials, so that the materials with different physical characteristics, such as high-viscosity humus, peat soil and a granular additive with strong flowability, are fully mixed, the mixing uniformity is improved, the materials can reach a uniform mixing state more quickly, the single mixing period is shortened, and the stirring effect and efficiency are improved.

The present application relates to solid waste resource utilization technical field, the present application discloses a kind of preparation method and application of artificial organic soil based on yellow phosphorus slag, comprising the following steps: 1) modified yellow phosphorus slag, 2) modified humus, 3) compounding, the modified yellow phosphorus slag is mixed with modified humus, appropriate amount of water is added while mixing, stationary, obtain mixed material, drying, by pulverizer grinding and mixer stirring, obtain artificial organic soil;The present application solves the problem of soil resource deficiency and yellow phosphorus slag resource utilization, not only can improve the capacity of soil resources, but also can break through the bottleneck of yellow phosphorus slag resource utilization.

The application belongs to the technical field of catalyst materials, and particularly relates to a sulfur and nitrogen co-doped carbon catalyst as well as a preparation method and application thereof. The sulfur and nitrogen co-doped carbon catalyst contains 0.18-4.4wt% of S and 0.08-7.0wt% of N. The preparation method of the sulfur and nitrogen co-doped carbon catalyst comprises the following steps: mixing a nitrogen-containing precursor, a sulfur-containing precursor and humin, and calcining to obtain the sulfur and nitrogen co-doped carbon catalyst. The sulfur and nitrogen co-doped carbon catalyst has Lewis acid-base double active centers by introducing sulfur and nitrogen into the carbon material, wherein S is an acid active center and N is an alkaline active center, and can fully activate epoxide and carbon dioxide, respectively, and has good catalytic performance and reusability.

The invention belongs to the technical field of soil amendments, and particularly relates to an acid soil amendment based on coal water slurry gasification slag and a preparation method and an amendment method of the acid soil amendment. The acid soil improver based on the coal water slurry gasified slag comprises the following components in parts by mass: 10-60 parts of gasified coarse slag, 0-20 parts of gasified fine slag, 1-5 parts of bentonite and 5-10 parts of humus, and the humus is obtained by fermenting pigeon manure and chicken manure with bacillus amyloliquefaciens, then adding bacillus subtilis, fermenting and sterilizing. The coal chemical industry solid waste gasification slag is used as a core improvement raw material, and is matched with the humus and the bentonite, so that the physical and chemical properties of the acid soil can be improved, the problems that the gasification slag occupies land and pollutes the environment due to long-term stockpiling are solved, and the resource recycling of industrial wastes is realized.

The application discloses a kind of closed field leachate and kitchen garbage combined anaerobic digestion device and method, device includes sequentially and side by side and is connected and is arranged pretreatment bin, preliminary mixing bin and anaerobic digestion bin, preliminary mixing bin middle part is equipped with a for receiving the mixed component of solid kitchen garbage on the filter plate.S1, kitchen garbage pretreatment;S2, kitchen garbage humification treatment;S3, combined anaerobic digestion treatment;S4, gas collection.The application is by closed field leachate as inoculum, kitchen garbage is digestion material, carries out sequential batch combined anaerobic digestion treatment, so that closed field leachate has good inoculation function, under the condition that no any inoculum is added, combined anaerobic digestion system can quickly enter gas production peak period finally by kitchen garbage conversion formed humus is substantially completely degraded.

The invention relates to the technical field of biotechnology, and discloses a microbial cellulose straw degradation, separation and screening method which comprises the following steps: performing multi-stage screening on strains, collecting humus soil samples, and performing three-stage screening on the humus soil samples through a cellulose-Congo red double-layer flat plate primary screening, liquid fermentation secondary screening and enzyme activity quantitative fine screening three-stage screening system. The preparation method comprises the following steps: preparing a cellulose degradation bacterial strain, separating to obtain a cellulose degradation bacterial strain, pretreating straws, crushing corn straws until the particle size is 0.5-2mm, removing a lignin barrier by adopting a diluted acid-alkali combined treatment method, and preparing a composite microbial agent; according to the method disclosed by the invention, a three-stage screening system of cellulose-Congo red double-layer flat plate primary screening, liquid fermentation secondary screening and enzyme activity quantitative fine screening is constructed, so that the capture precision of the efficient degradation strain is improved; the composite mutagenesis technology induces the bacterial strain to generate functional mutation, and the specific compounding proportion of the mutant strain and the original bacterial strain is combined to form a composite bacterial agent with a synergistic effect, so that the natural barrier of lignin-cellulose in the straw is broken, and the biodegradation efficiency of the cellulose is enhanced.

The method for producing an aqueous solution of hydroxymethylfurfural (5-HMF) comprises step a) of contacting a feedstock (1) containing 5-HMF and a polar aprotic synthetic solvent with an aqueous stream (21), step b) of liquid-liquid extraction with an organic extract (6), followed by step c) of backwashing with an aqueous solvent (7) to obtain an organic raffinate enriched in an aqueous solution of 5-HMF (12) and the organic solvent. The optionally concentrated organic raffinate (10) is then subjected to step e) of hydrodistillation to obtain an aqueous solution of 5-HMF. Step f) of liquid-solid separation of the aqueous raffinate (5) obtained in step b) and / or the solid particle fraction formed by precipitated humins in the intermediate stream (19) obtained in step b) is then carried out. The aqueous raffinate obtained in step b), from which precipitated humins have been removed, is then treated in step g) with a water-polar aprotic synthetic solvent mixture.