Paracoccus aminovorans HPD-2 and use thereof in soil remediation

A technology of Paracoccus ammoniaphaga and HPD-2, which is applied in the field of Paracoccus ammoniaphaga, can solve the problem of not finding microbial resources that degrade multiple high molecular weight PAHs at the same time.

Active Publication Date: 2009-01-21
中科华鲁土壤修复工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No microbial resources capable of simultaneously degrading multiple high-molecular-weight PAHs have been found so far

Method used

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  • Paracoccus aminovorans HPD-2 and use thereof in soil remediation
  • Paracoccus aminovorans HPD-2 and use thereof in soil remediation
  • Paracoccus aminovorans HPD-2 and use thereof in soil remediation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Isolation, identification and degradation characteristics of Paracoccus aminovorans HPD-2 CGMCC No.2568

[0020] 1.1 Test soil

[0021] It was collected from a soil heavily polluted by polycyclic aromatic hydrocarbons in a certain place in the Yangtze River Delta. Its basic physical and chemical properties are: pH 6.4, organic matter 19.2g / kg, alkali-hydrolyzed nitrogen 1.0g / kg, total phosphorus 0.5g / kg, available potassium 14.2g / kg, cation exchange capacity 21.5cmol / kg. The total amount of 16 kinds of PAHs identified as priority pollutants by EPA reached 10.3mg / kg. Fresh soil samples were passed through a 2mm sieve and stored in the dark at 4°C.

[0022] 1.2 Tested polycyclic aromatic hydrocarbons

[0023] B[a]P, PYR and FA were all purchased from Sigma Company (USA) and were of analytical grade.

[0024] 1.3 Culture Type

[0025] ①Beef extract peptone medium; ②Inorganic salt medium (MS): K per liter 2 HPO 4 6.0g, KH 2 PO 4 5.5g, Na 2 SO 4 2.0g...

Embodiment 2

[0048] Example 2: Soil remediation effect of Paracoccus aminovorans HPD-2 CGMCC No.2568

[0049] 1.1 Test soil

[0050] Collected from PAH-contaminated soil somewhere in the Yangtze River Delta. The basic physical and chemical properties of the soil are: pH 6.4, organic matter 19.2g / kg, alkaline hydrolyzed nitrogen 1.0g / kg, total phosphorus 0.5g / kg, available potassium 14.2g / kg, cation exchange capacity 21.5cmol / kg. The total amount of 9 kinds of PAHs identified as priority pollutants by EPA reached 10.78mg / kg. Among them, the content of tetracyclic and pentacyclic PAHs accounted for 71.8% and 23.0% of the total respectively, indicating that the PAHs in the soil were mainly composed of high molecular weight PAHs. Fresh soil samples were passed through a 2mm sieve and stored in the dark at 4°C.

[0051] 1.2 Types of cultivation

[0052] ①Beef extract peptone medium; ②Inorganic salt medium (MS): K per liter 2 HPO 4 6.0g, KH 2 PO 4 5.5g, Na 2 SO 4 2.0g, KCl 2.0g, NH ...

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Abstract

The invention relates to a Paracoccus aminovorans HPD-2. The strain has been preserved in a depository authority specified by the State Intellectual Property Office; the collection date is July, 3rd, 2008; the name of the depository authority is China General Microbiological Culture Collection Center of the China Committee of Culture Collection for Microorganisms; and the collection number is CGMCC No.2568. The degrading bacterium has wide substrate spectrum and good degradability on PAHs in the environment.

Description

1. Technical field [0001] The invention relates to paracoccus ammoniaphagia, in particular to paracoccus ammoniaphagus HPD-2 and its application in remediation of polycyclic aromatic hydrocarbon polluted soil. 2. Background technology [0002] Polycyclic aromatic hydrocarbons (PAHs) are a class of organic pollutants ubiquitous in the environment. Due to their long half-life in the environment and their carcinogenic, teratogenic, and mutagenic properties, people have paid attention to them. In the early 1980s, the U.S. Environmental Protection Agency identified 16 unbranched PAHs as priority pollutants in the environment, and my country also included them in the blacklist of environmental pollution. Benzo[a]pyrene (B[a]P) is a polycyclic aromatic hydrocarbon compound composed of five benzene rings. It is extremely carcinogenic and is one of the important indicators for environmental monitoring at home and abroad. Pyrene (PYR) and fluoranthene (Fluoranthene, FA) are both tetr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12S13/00C12R1/01C12S99/00
Inventor 骆永明毛健滕应李振高
Owner 中科华鲁土壤修复工程有限公司
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