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45 results about "TetR" patented technology
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Tet Repressor proteins (otherwise known as TetR) are proteins playing an important role in conferring antibiotic resistance to large categories of bacterial species. Tetracycline (tc) is a broad family of antibiotics to which bacteria have evolved resistance. Tc normally kills bacteria by binding to the bacterial ribosome and halting protein synthesis. The expression of tc resistance genes is regulated by Tet Repressor Protein. More specifically, TetR represses the expression of TetA, a membrane protein that pumps out substances toxic to the bacteria like tc, by binding the TetA operator. In tc resistant bacteria, TetA will pump out tc before it can bind to the ribosome because the repressive action of TetR on TetA is halted by binding of tc to TetR. Therefore, TetR may have an important role in helping scientists to better understand mechanisms of antibiotic resistance and how to treat antibiotic resistant bacteria. TetR is one of many proteins in the TetR protein family, which is so named because TetR is the most well characterized member.
Method for propagating adenoviral vectors encoding inhibitory gene products
The invention provides a method of propagating an adenoviral vector. The method comprises (a) providing a cell comprising a cellular genome comprising a nucleic acid sequence encoding a tetracycline operon repressor protein (tetR), and (b) contacting the cell with an adenoviral vector comprising a heterologous nucleic acid sequence encoding a toxic protein. The heterologous nucleic acid sequence is operably linked to a promoter and one or more tetracycline operon operator sequences (tetO), and expression of the heterologous nucleic acid sequence is inhibited in the presence of tetR, such that the adenoviral vector is propagated. The invention also provides a system comprising the aforementioned cell and adenoviral vector.
Owner:GEN VEC INC
High anti-wear and antistatic polyurethane materials
InactiveCN1664007AUnique preparation processSimple preparation processOther chemical processesPolyesterHigh resistance
The invention relates to a method for preparing a new material, particularly high-wearing and antistatic polyurethane material. The polyurethane material is formed by tetr-zinc oxide(T-ZnO)whisker and polyester polyol or polyether polyol, toluene diisocyanate(TDI) or methyl diphenylene diisocyanate(MDI) or p- phenyldiisocyanate(PPDI) and 3, 3'-dichlor-4, 4'- diaminodiphenylmethane(MOCA). The product can be got from the tetr-zinc oxide whisker through casting process after the decentralized and coupling processing. The invention is characterized by the unique and simple preparing process, the superior quality, high-wearing feature, high resistance to ripping, antistatic property, ageing and heating resistance; and the lower cost compared with the traditional polyurethane material. The product can be exclusively used in machinery, aerospace engineering, light industry, architecture, textile and mine engineering and so on.
Owner:SHENYANG INSTITUTE OF CHEMICAL TECHNOLOGY
Method for increasing erythromycin yield by transforming saccharopolyspora erythraea SACE_3980 gene
ActiveCN106520866AImprove fermentation yieldIncrease productionFungiMicroorganism based processesBiotechnologyTetR
The invention discloses a method for increasing the erythromycin yield by transforming the saccharopolyspora erythraea SACE_3980 gene. In saccharopolyspora erythraea, the gene SACE_3980 is subjected to transcription regulation by genetic engineering and through TetR-deficient family to obtain an engineering strain with high erythromycin yield; and by adopting the obtained strain for fermentation production of erythromycin, the yield can be remarkably increased, and a new technical support is provided for increasing the erythromycin yield in industrial production.
Owner:ANHUI UNIVERSITY
Method for improving erythrocin yield through inactivation saccharopolyspora erythraea SACE_3446 gene
The invention discloses a method for improving erythrocin yield through a negative control gene SACE_3446 on an inactivation saccharopolyspora erythraea chromosome. Saccharopolyspora erythraea is used for producing erythrocin. The erythrocin and derived drugs of the erythrocin such as clarithromycin, azithromycin and telithromycin are used widely in clinic. Erythrocin high-producing strain screening is very important in industrial production. The erythromycin biosynthesis negative control gene SACE_3446 is screened from a saccharopolyspora erythraea TetR family. Compared with erythrocin yield of an original strain, deletion mutants of the saccharopolyspora erythraea SACE_3446 is improved remarkably, the erythrocin is returned to low yield after gene complementation of the SACE_3446, and therefore the SACE_3446 gene is a erythromycin biosynthesis negative control gene. The inactivation saccharopolyspora erythraea SACE_3446 gene can improve the erythrocin yield through a genetic engineering way. Due to the fact that erythromycin biosynthesis gene control is a network system, upstream and downstream control factors acted by SACE_3446 control factors can be found by using the SACE_3446 as an object. The erythrocin yield can also be improved by changing upstream or downstream control factor genes of the saccharopolyspora erythraea SACE_3446 control factors.
Owner:ANHUI UNIVERSITY
Crystalline form of (s)-7-(1-acryloylpiperidin-4-yl)-2-(4-phenoxyphenyl)-4,5,6,7-tetra-hydropyrazolo[1,5-a]pyrimidine-3-carboxamide, preparation, and uses thereof
ActiveUS20190169201A1High melting pointStable profileOrganic active ingredientsAntipyreticDiseaseMedicine
The present invention relates to a crystalline form of (S)-7-(1-acryloylpiperidin-4-yl)-2-(4-phenoxyphenyl)-4,5,6,7-tetr a-hydropyrazolo[1,5-a]pyrimidine-3-carboxamide for inhibiting Btk, methods of preparation thereof and pharmaceutical compositions, and use of the crystalline form above in the treatment of a disease, or in the manufacturing of a medicament for the treatment of a disease.
![Crystalline form of (s)-7-(1-acryloylpiperidin-4-yl)-2-(4-phenoxyphenyl)-4,5,6,7-tetra-hydropyrazolo[1,5-a]pyrimidine-3-carboxamide, preparation, and uses thereof](https://images-eureka.patsnap.com/patent_img/f182233b-7a56-4f96-8c3e-40c92b7e4200/US20190169201A1-D00001.png "Crystalline form of (s)-7-(1-acryloylpiperidin-4-yl)-2-(4-phenoxyphenyl)-4,5,6,7-tetra-hydropyrazolo[1,5-a]pyrimidine-3-carboxamide, preparation, and uses thereof")
![Crystalline form of (s)-7-(1-acryloylpiperidin-4-yl)-2-(4-phenoxyphenyl)-4,5,6,7-tetra-hydropyrazolo[1,5-a]pyrimidine-3-carboxamide, preparation, and uses thereof](https://images-eureka.patsnap.com/patent_img/f182233b-7a56-4f96-8c3e-40c92b7e4200/US20190169201A1-D00002.png "Crystalline form of (s)-7-(1-acryloylpiperidin-4-yl)-2-(4-phenoxyphenyl)-4,5,6,7-tetra-hydropyrazolo[1,5-a]pyrimidine-3-carboxamide, preparation, and uses thereof")
![Crystalline form of (s)-7-(1-acryloylpiperidin-4-yl)-2-(4-phenoxyphenyl)-4,5,6,7-tetra-hydropyrazolo[1,5-a]pyrimidine-3-carboxamide, preparation, and uses thereof](https://images-eureka.patsnap.com/patent_img/f182233b-7a56-4f96-8c3e-40c92b7e4200/US20190169201A1-D00003.png "Crystalline form of (s)-7-(1-acryloylpiperidin-4-yl)-2-(4-phenoxyphenyl)-4,5,6,7-tetra-hydropyrazolo[1,5-a]pyrimidine-3-carboxamide, preparation, and uses thereof")
Owner:BEIGENE SWITZERLAND GMBH
Recombinant bacteria for fine regulation and control of saccharomyces cerevisiae ERG7 expression and construction method thereof
The invention discloses recombinant bacteria for fine regulation and control of saccharomyces cerevisiae ERG7 expression and a construction method thereof. The construction method comprises the following steps of: (1) constructing a TetR gene expression cassette ACS1p-TetR-Cy1t, and transferring the cassette into saccharomyces cerevisiae; (2) inserting a TetO sequence into places from an initial codon to -78 bp of a saccharomyces cerevisiae ERG7p promoter so as to obtain a saccharomyces cerevisiae ERG7p promoter mutant ERG7Cp; and (3) replacing the endogenous ERG7p promoter in the saccharomyces cerevisiae obtained in the step (1) by the ERG7Cp to obtain the recombinant bacteria for fine regulation and control of saccharomyces cerevisiae ERG7 expression. According to the invention, a tetracycline repressor protein TetR and a TetO sequence control element are utilized to realize fine control on ERG7 expression. The constructed recombinant bacteria for fine regulation and control of saccharomyces cerevisiae ERG7 expression can inhibit the expression of the ERG7 gene in different degrees and improve the yield of engineering saccharomyces cerevisiae protopanaxadiol.
Owner:TIANJIN UNIV
Highly inducible dual-promoter lentiviral tet-on system
The invention relates to expression systems useful for regulated expression of a gene of interest based on the constitutive expression of the original TetR repressor and the expression of the polynucleotide driven by a constitutive promoter operably linked to an operator sequence for a tetracycline operator sequence. The system can be provided as two different polynucleotides or as an all-in-one vector. The invention also relates to vectors, host cells and viral particles according to the invention as well as to the uses thereof for in vitro and in vivo production of products of interest or for therapy.
Owner:FUNDACION PUBLICA ANDALUZA PROGRESO & SALUD +1
Method for increasing yield of erythromycin by transforming streptomyces erythraea SACE_0303 gene
ActiveCN110157756AIncrease productionStable introduction of DNAMicroorganism based processesMicrobiologyGenetic engineering
The invention discloses a method for increasing the yield of erythromycin by transforming a streptomyces erythraea SACE_0303 gene. A positive regulator SACE_0303 is biosynthesized by utilizing the screened erythromycin, the TetR family transcriptional SACE_0303 gene in the streptomyces erythraea is overexpressed by means of genetic engineering, an erythromycin high-yield engineering strain of thestreptomyces erythraea is obtained, and fermented to produce the erythromycin, and technical support is provided for increasing the yield of the erythromycin through industrial production.
Owner:ANHUI UNIVERSITY
Interferon-beta production modulating listeria strains and methods for using same
ActiveUS20100285067A1Increase productionReduce productionAntibacterial agentsBiocideBacteroidesListeria floridensis
Mutant Listeria bacteria that modulate interferon-β production are provided. The subject bacteria are characterized by having a mutation in a gene chosen from a TetR gene, a LadR gene, a VirR gene, a MarR gene a MdrL gene, a MdrT gene and a MdrM gene. The subject bacteria find use in a variety of applications, where representative applications of interest include, but are not limited to: (a) use of the subject bacteria as adjuvants; (b) use of the subject bacteria as delivery vectors for introducing macromolecules into a cell; (c) use of the subject bacteria as vaccines for eliciting or boosting a cellular immune response; etc.
Owner:RGT UNIV OF CALIFORNIA
A kind of 1,3-propanediol-producing genetically engineered bacteria and its preparation method and application
InactiveCN101260379BImprove stabilityHigh final concentrationBacteriaMicroorganism based processesMicroorganism1,3-Propanediol
Owner:NANJING TECH UNIV
Tet transactivator system
InactiveUS20030185851A1Prevent normal gene functionDisrupting functionOrganic active ingredientsProtozoa antigen ingredientsBiotechnologyTetR
A transcriptional activator of T. gondii is provided which comprises the tetracycline repressor (TetR) operatively linked to a transacting factor of T. gondii. Strains of T. gondii transformed with a vector containing such a transactivator may be used to prepare vaccine compositions or to identify essential genes in the parasite. The system provided may be useful in other Apicomplexan species such as Plasmodium falciparum, Plasmodium vivax, Plasmodium berghei, Plasmodium yoelii, Plasmodium knowlesi, Trypanosoma brucei, Entamoeba histolytica, and Giardia lambia.
Owner:IMPERIAL INNOVATIONS LTD
Protein TetR combinable with tetracycline and coding genes and applications of protein TetR combinable with tetracycline
InactiveCN102690338AEfficient preparationHigh sensitivityBacteriaMicroorganism based processesEscherichia coliBiotechnology
The invention discloses a protein TetR combinable with tetracycline and coding genes and applications of the protein TetR combinable with tetracycline. The protein which can be specifically combined with the tetracycline is (a) or (b): (a) is a protein composed by the amino acid sequence shown by sequence 1 in the sequence list, and (b) is a protein derived from (a) by subjecting the amido acid residue sequence of the sequence 1 in the sequence list to substitution and / or deletion and / or addition of one or multiple amido acid residues and by combination with tetracycline antibiotics. Recombined escherichia coli can be obtained by leading coding genes of tetracycline receptor protein into starting escherichia coli. The invention further discloses a method for preparing the protein, namely cultivation of the recombined strain. Experiments show that the method for preparing the tetracycline receptor protein is high in yield, and problems of low yield and difficulty in preparation in the prior art are solved, so that the method is of great importance.
Owner:CHINA AGRI UNIV
Method for improving yield of lincomycin through streptomyces lincolnensis regulation gene combination modification
ActiveCN112251456AIncrease productionImprove fermentation yieldPeptidesFermentationBiotechnologyResistant genes
The invention discloses a method for improving the yield of lincomycin through streptomyces lincolnensis regulation gene combination transformation. Strains are obtained by knocking out an Lrp familytranscription regulatory gene SLCG-4846 and a TetR family transcription regulatory gene SLCG-2919 from streptomyces lincolnensis in a combined manner. Meanwhile, the invention further discloses the method for improving the yield of lincomycin through streptomyces lincolnensis regulation gene combination modification. According to the method, traceless knockout is sequentially carried out on a plurality of genes through a homologous recombination technology, and resistance genes are not introduced in the process, so that the fermentation cost is reduced, and the hereditary stability is guaranteed. Meanwhile, the process does not influence the growth of thalli, and most importantly, multiple knockout experiments can be continuously carried out in the same strain. According to the method, theyield of lincomycin can be greatly increased, and a new technical support is provided for improving the yield of lincomycin in industrial production.
Owner:ANHUI UNIVERSITY
Method capable of improving homologous recombination efficiency of CRISPR/Cas9 system and application
InactiveCN113667017AIncrease positive rateIncrease the positive rate of homologous recombinationVectorsHydrolasesOperonGene Modification
The invention provides a method capable of improving the homologous recombination efficiency of a CRISPR / Cas9 system and application. The CRISPR / Cas9 system comprises a Cas9-TetR fusion protein, guide RNA and a vector containing a tetracycline operon (tetO) element. The invention provides the method for carrying out homologous recombination by utilizing the CRISPR / Cas9 system. According to the method, a recombinant vector or Cas9 protein does not need to be subjected to additional chemical modification and purification, and the homologous recombinant vector only needs to be constructed into the skeleton vector containing the tetO element like conventional vector construction; the positive rate of homologous recombination of mediated DNA large fragments (more than 2000 base pairs) can be improved; and the homologous recombination positive rate can be improved by 2-3 times in the preparation of a gene modification animal model.
Owner:SHANGHAI BIOMODEL ORGANISM SCI & TECH DEV +2
Conditionally replicating adenovirus vector for viral replication regulated by transcription inhibition type Tet-On system and application
The invention discloses a conditionally replicating adenovirus vector for virus replication regulated by a TetR-KRAB-mediated transcription inhibition type Tet-On system, adenovirus E1 region comprises the following components in the following connection sequence: 5'-CMV promoter, TetR-KRAB gene, promoter TRE3G-E1b Pro containing a tetracycline responsive element, EcoR I enzyme cutting site, E1B Delta 55KD protein-deleted adenovirus E1A-E1B19KD gene sequence, enzyme cutting site Spe I and mRNA transcription termination signal SV40polyA-3'; the CMV promoter expresses transcription inhibitor TetR-KRAB; a gene sequence inserted into the two enzyme cutting sites of the EcoR I and the Spe I is a gene fragment from adenovirus E1A translation initiation site to adenovirus E1B19KD protein termination codon, the promoter TRE3G-E1b Pro containing the tetracycline responsive element expresses adenovirus E1A gene, adenovirus E1B19KD gene is expressed by the own promoter of the adenovirus E1B19KD, and the packed adenovirus is named as Ad5-CMV-TetR-KRAB-TRE3G-E1bPro-Delta 55KD. Tests show that the virus can be loaded into mesenchymal stem cells for application in study of tumor therapy.
Owner:SHAANXI NORMAL UNIV
Dehydrated tetracycline-induced escherichia coli-bacillus subtilis universal inducible expression system
ActiveCN114645062AReduce leakageHigh induction magnificationMicroorganism based processesNucleic acid vectorEscherichia coliHeterologous
The invention discloses a dehydration tetracycline induced escherichia coli-bacillus subtilis universal type inducible expression system, and belongs to the field of gene engineering. According to the invention, an operon Pet expression system capable of using anhydrotetracycline to induce expression in escherichia coli and bacillus subtilis is constructed, and the system takes a commercial plasmid pHT01 as an expression vector and comprises a repressor protein tetR expression cassette and a super-folded green fluorescent protein (sfGFP) expression cassette. Wherein the gene sequence and the RBS sequence of the repressor protein tetR in the expression cassette of the repressor protein tetR are mutated to change the affinity and the expression intensity of the repressor protein. The operon Pet expression system shows low leakage and high induction magnification times in escherichia coli and bacillus subtilis. Therefore, the general expression system is simple in structure, high in activity and rigorous in regulation and control, and has a wide application prospect in heterologous protein high-efficiency expression and synthetic biology research.
Owner:JIANGNAN UNIV
Vector for the inducible expression of gene sequences
InactiveUS20070122880A1Cell receptors/surface-antigens/surface-determinantsSugar derivativesTetRTransgene
The invention relates to a nucleic acid construct that comprises a human EF-1α promoter operatively associated with the tetracycline repressor (TetR). The invention further provides cell lines that have been stably transfected with such construct, as well as methods of using said construct, for the inducible expression of a transgene or for the inducible silencing of a endogenous sequence in mammalian cells.
Owner:INSTITUT CURIE
Method for one-step transformation and marking of blue-green algae chromosomes and application
InactiveCN113046382AStable introduction of DNAVector-based foreign material introductionBiotechnologyOperon
The invention discloses a method for one-step transformation and marking of blue-green algae chromosomes and application. The method comprises the following steps: combining specificity of an inhibiting protein TetR in a tetracycline operon with a manipulated DNA sequence TetO; transferring a TetR-GFP fusion protein and a TetOxn sequence array onto blue-green algae genome chromosomes through one-step genetic transformation; then inducing expression of the TetR-GFP fusion protein by utilizing a promoter; combining the expressed TetR-GFP fusion protein with a TetOxn sequence on the chromosomes; and finally, obtaining a blue-green algae positive strain with the chromosomes emitting green fluorescence. The number and dynamic information of the chromosomes can be observed under a fluorescence microscope, so that the chromosome fluorescence-marked algae strain can be used as an ideal material for researching a copying and separating mechanism of the blue-green algae chromosomes.
Owner:PEKING UNIV
Oscillating gene expression system, construction method and application of oscillating gene expression system in rhamnolipid fermentation
PendingCN113122556AIncrease productionRelieve supply pressureBacteriaMicroorganism based processesEscherichia coliHeterologous
The invention discloses an oscillating gene expression system, a construction method and application of the oscillating gene expression system in rhamnolipid fermentation. The oscillating gene expression system comprises a promoter PluxI, a repressor gene tetR, a promoter Pet, response regulatory factor genes LuxI and LuxR, and a protein degradation tag gene ssrA. By regulating the periodic switching process of gene expression, the oscillating gene expression system can relieve the supply pressure of metabolic intermediate products and energy of escherichia coli in a stable growth period, so that the gene expression and substrate supply are balanced, and the yield of secondary metabolites is further improved. According to the invention, by introducing a rhamnolipid biosynthetic pathway related gene into an oscillating gene expression regulatory plasmid, heterologous synthesis of rhamnolipid in Escherichia coli is realized, and the fermentation yield of the engineering bacteria of the oscillating gene expression system for high yield of rhamnolipid is up to 838mg / L.
Owner:NANJING UNIV OF SCI & TECH
Interferon-β production modulating Listeria strains and methods for using same
ActiveUS8277797B2Increase productionReduce productionAntibacterial agentsBiocideAdjuvantCell immunity
Mutant Listeria bacteria that modulate interferon-β production are provided. The subject bacteria are characterized by having a mutation in a gene chosen from a TetR gene, a LadR gene, a VirR gene, a MarR gene a MdrL gene, a MdrT gene and a MdrM gene. The subject bacteria find use in a variety of applications, where representative applications of interest include, but are not limited to: (a) use of the subject bacteria as adjuvants; (b) use of the subject bacteria as delivery vectors for introducing macromolecules into a cell; (c) use of the subject bacteria as vaccines for eliciting or boosting a cellular immune response; etc.
Owner:RGT UNIV OF CALIFORNIA
Method for improving yield of erythromycin by modifying saccharopolyspora erythraea SACE_4682 gene
ActiveCN111139192AImprove fermentation yieldIncrease productionFungiMicroorganism based processesMicrobiologyRegulator gene
The invention discloses a method for improving the yield of erythromycin by modifying a saccharopolyspora erythraea SACE_4682 gene. In the saccharopolyspora erythraea, a TetR family transcription regulation gene SACE_4682 is deleted by a genetic engineering method to obtain an erythromycin high-yield engineering strain, and the obtained strain is fermented to produce the erythromycin, so that a new technical support is provided for improving the yield of the erythromycin in industrial production.
Owner:ANHUI UNIVERSITY
Self-clearing type adenovirus vector and preparation method thereof
InactiveCN104178513ASurvival is not affectedUnaffected replicationViruses/bacteriophagesGenetic engineeringVector systemResponse element
The invention provides a self-clearing type adenovirus vector which is modified based on an AdEasyTM adenovirus vector system, wherein a used shuttle plasmid is pShuttle-CMV. The self-clearing type adenovirus vector is characterized in that the modification refers to inserting an rtetR (reverse tetR)-VP16 gene sequence, a TRE (Tetracyclin response element)-PminCMV-Cre gene sequence and two homodromous LoxP sites into the shuttle plasmid pShuttle-CMV. The self-clearing type adenovirus vector can be used for inducing adenoviruses to degrade and die at any time, can be used for eliminating exogenous genes and the adenovirus vector body before seed cells needed for tissue engineering are applied in vivo, and can be used for greatly lowering the quantity of the adenoviruses that enter the organisms to relieve influences of the vector on the organisms, so that a relatively safe and reliable vector is provided for a tissue engineering product.
Owner:THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV +1
Method for propagating adenoviral vectors encoding inhibitory gene products
The invention provides a method of propagating an adenoviral vector. The method comprises (a) providing a cell comprising a cellular genome comprising a nucleic acid sequence encoding a tetracycline operon repressor protein (tetR), and (b) contacting the cell with an adenoviral vector comprising a heterologous nucleic acid sequence encoding a toxic protein. The heterologous nucleic acid sequence is operably linked to a promoter and one or more tetracycline operon operator sequences (tetO), and expression of the heterologous nucleic acid sequence is inhibited in the presence of tetR, such that the adenoviral vector is propagated. The invention also provides a system comprising the aforementioned cell and adenoviral vector.
Owner:GEN VEC INC
Engineering bacterium for producing daptomycin and application thereof
ActiveCN114561332AIncrease productionReduce manufacturing costBacteriaMicroorganism based processesBiotechnologyMicrobiology
The invention relates to an engineering bacterium for producing daptomycin and application of the engineering bacterium. The invention provides an engineering bacterium for producing daptomycin. A coding gene of a TetR family transcriptional regulation factor in the engineering bacterium is inactivated or expression of the TetR family transcriptional regulation factor is reduced. The invention also provides a preparation method and application of the engineering bacterium.
Owner:CHINA AGRI UNIV
Engineering strain for controlling adsorption and escape of heavy metals as well as construction method and application of engineering strain
PendingCN113999864ATo achieve simultaneous useRealize precise regulationBacteriaWater contaminantsEscherichia coliPseudomonas putida
The invention discloses an engineering strain for controlling adsorption and escape of heavy metals as well as a construction method and an application of the engineering strain. The engineering strain is a bacterium obtained by introducing a complete system into a host bacterium; the complete system comprises a heavy metal ion regulation protein, a heavy metal ion adsorption protein displayed on the surface of an ice nucleation protein, a streptavidin binding protein displayed on the surface of the ice nucleation protein and an escape control element, and the escape control element comprises repressor proteins PhlF, TetR, LacI and a lethal protein. Based on a synthetic biological technology, the invention provides a water body heavy metal adsorption and escape control microorganism which integrates the functions of heavy metal adsorption, microorganism enrichment after adsorption, escape control and the like in escherichia coli or pseudomonas putida, and the construction of a complex, fine and predictable genetic loop is realized by utilizing a standardized biological element; after optimization design, gene lines of adsorption, enrichment and death behaviors can be completed under the condition of mercury ions with different concentrations.
Owner:INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Treatment of ADHD
The use of 3-[1-[2-(1-acetyl-2,3-dihydro-1H-indol-3-yl)ethyl]-1,2,3,6-tetr ahydropyridin-4-yl]-6-chloro-1H-indole, any of its enantiomers and pharmaceutically acceptable salts thereof for the preparation of a pharmaceutical composition for the treatment of attention deficit hyperactivity disorder.
Owner:H LUNDBECK AS
Construction of broad-host plasmid for inducible expression of green fluorescent protein and application of broad-host plasmid in fluorescent tracing
PendingCN113444739AStrong fluorescent activityFacilitate fluorescent tracer studiesVectorsAnimals/human peptidesEscherichia coliBrucella
The invention discloses construction of a broad-host plasmid for inducible expression of green fluorescent protein and application of the broad-host plasmid in fluorescent tracing. A pBT-iEGFP plasmid disclosed by the invention is constructed on the basis of a broad-host plasmid pBBR1MCS skeleton, and comprises sequences of a tetracycline repressor protein TetR, a tetracycline promoter Pzt-1 and an enhanced green fluorescent protein EGFP. The broad-host plasmid for inducible expression of green fluorescent protein has the characteristic of broad hosts, can be copied at least in escherichia coli bacteria, salmonella bacteria and brucella bacteria, but is not limited to the escherichia coli bacteria, the salmonella bacteria and the brucella bacteria. Bacteria containing the plasmid can induce host bacteria to emit green fluorescence under the induction condition of anhydrous tetracycline with specific concentration. The broad-host plasmid for inducible expression of green fluorescent protein has the advantages of versatility of multiple bacteria, controllable expression, specific tracing of viable bacteria, strong fluorescence and the like. The plasmid can be used for fluorescence labeling of various bacteria, bacterial morphological observation, fluorescence dynamic tracing and other technologies.
Owner:SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
Tetracycline drug detection method based on TetR protein steric hindrance and gene cleavage technology
PendingCN113957067AEnables multi-residue detectionEasy to operatePeptidesFluorescence/phosphorescenceFluorophoreBiotin
The invention discloses a tetracycline drug detection method based on TetR protein steric hindrance and a gene cleavage technology, a double-stranded DNA molecule for detecting tetracycline drugs, a composition, a kit and application of the double-stranded DNA molecule. The double-stranded DNA molecule comprises a tetracycline manipulating gene tetO and a restriction endonuclease recognition site, one end of the double-stranded DNA molecule is connected with a fluorophore, and the other end of the double-stranded DNA molecule is connected with biotin. The double-stranded DNA molecule can realize detection of tetracycline drugs.
Owner:CHINA AGRI UNIV
Conditional replicative adenoviral vector and application of viral replication regulated by transcriptional repressive tet-on system
The invention discloses a conditionally replicating adenovirus vector for virus replication regulated by a TetR-KRAB-mediated transcription inhibition type Tet-On system, adenovirus E1 region comprises the following components in the following connection sequence: 5'-CMV promoter, TetR-KRAB gene, promoter TRE3G-E1b Pro containing a tetracycline responsive element, EcoR I enzyme cutting site, E1B Delta 55KD protein-deleted adenovirus E1A-E1B19KD gene sequence, enzyme cutting site Spe I and mRNA transcription termination signal SV40polyA-3'; the CMV promoter expresses transcription inhibitor TetR-KRAB; a gene sequence inserted into the two enzyme cutting sites of the EcoR I and the Spe I is a gene fragment from adenovirus E1A translation initiation site to adenovirus E1B19KD protein termination codon, the promoter TRE3G-E1b Pro containing the tetracycline responsive element expresses adenovirus E1A gene, adenovirus E1B19KD gene is expressed by the own promoter of the adenovirus E1B19KD, and the packed adenovirus is named as Ad5-CMV-TetR-KRAB-TRE3G-E1bPro-Delta 55KD. Tests show that the virus can be loaded into mesenchymal stem cells for application in study of tumor therapy.
Owner:SHAANXI NORMAL UNIV
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