Recombinant bacteria for fine regulation and control of saccharomyces cerevisiae ERG7 expression and construction method thereof

A technology for Saccharomyces cerevisiae and a construction method, which is applied in the field of bioengineering and can solve the problems of increasing the cost of raw materials, the complexity of fermentation control, and the disadvantages.
CN110628805AInactive Publication Date: 2019-12-31TIANJIN UNIV
1 Cites 9 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TIANJIN UNIV
Publication Date
2019-12-31
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses recombinant bacteria for fine regulation and control of saccharomyces cerevisiae ERG7 expression and a construction method thereof. The construction method comprises the following steps of: (1) constructing a TetR gene expression cassette ACS1p-TetR-Cy1t, and transferring the cassette into saccharomyces cerevisiae; (2) inserting a TetO sequence into places from an initial codon to -78 bp of a saccharomyces cerevisiae ERG7p promoter so as to obtain a saccharomyces cerevisiae ERG7p promoter mutant ERG7Cp; and (3) replacing the endogenous ERG7p promoter in the saccharomyces cerevisiae obtained in the step (1) by the ERG7Cp to obtain the recombinant bacteria for fine regulation and control of saccharomyces cerevisiae ERG7 expression. According to the invention, a tetracycline repressor protein TetR and a TetO sequence control element are utilized to realize fine control on ERG7 expression. The constructed recombinant bacteria for fine regulation and control of saccharomyces cerevisiae ERG7 expression can inhibit the expression of the ERG7 gene in different degrees and improve the yield of engineering saccharomyces cerevisiae protopanaxadiol.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of bioengineering, and in particular relates to a recombinant bacterium and a construction method for finely regulating the expression of Saccharomyces cerevisiae ERG7. Background technique

[0002] Triterpenoids, such as protopanaxadiol, oleanolic acid, glycyrrhetinic acid, betulinic acid, ursolic acid, etc., are the main components of many Chinese herbal medicines to exert their medicinal activities, and are widely used in medicine, health products and food fields . At present, such compound products are mainly obtained through plant extraction. Taking protopanaxadiol as an example, ginsenosides in ginseng are extracted, and then obtained through acid-base hydrolysis or enzymatic hydrolysis. Traditional production methods have low extraction efficiency, long production cycle, high cost and great damage to the environment, which are far from meeting the demand for triterpenoids in the chemical and pharmace...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More