The invention discloses a Chinese-Western combination disinfectant for an operating room and a preparation method thereof, belonging to the technical field of medicine; the ingredients of the disinfectant in the invention consist of the following raw materials: Yeguanmen, Chicory, Dried Ginger, Panax notoginseng, Camphor leaves, Polygonum water, Sorrel, honeysuckle, green jasmine, hundred taels of gold, toon root bark, plum grass, cresol sulfonic acid, glutaraldehyde and povidone iodine; the disinfectant of the present invention is selected from the prescription of sterilization, anti-inflammation, insecticide and antipruritic, The combination of Chinese and Western preparations maximizes the efficacy of Chinese medicine ingredients and Western medicines, plays a complementary and enhancing role, improves medicinal effects, and can make enzymes, coenzymes and metabolic intermediates in the bacteria leak out, hindering bacterial respiration and glycolysis It can detoxify the process and denature the protein of bacteria, so as to play the role of sterilization. It has a wide and fast effect, strong efficacy, and treats both symptoms and root causes. It has an excellent effect on the sterilization and disinfection of the operating room.