34 results about "Lincomycin" patented technology

A veterinary medicine for preventing and treating the frequently encountered diseases of pig, such as metritis, mammitis, acyesis, sterility, dysentery, epidemic enteritis, paratyphoid, asthma, etc is prepared from 15 raw materials including florfenicol, amoxicillin, lucomycin, doxycycline, etc.

The invention relates to a magnetic immunochemiluminescence kit for detecting lincomycin, which comprises the following reagents, enzyme-labelled antigen, an enzyme-labelled antigen weak solution, a magnetic antibody, a magnetic antibody weak solution, a lincomycin series standard substance solution, a chemiluminescence substrate solution A, a chemiluminescence substrate solution B, a concentrated compound solution, and a concentrated washing liquid. enzyme-labelled antigen is a horseradish peroxidase-labelled lincomycin hapten marker, lincomycin hapten is keto lincomycin obtained by performing pyridinium dichromate cas oxidation on alcoholic hydroxyl group from lincomycin, keto lincomycin and aminobutyric acid are subjected to a condensation reaction to obtain four-carbon chain carboxyl hapten, the magnetic antibody is obtained by coupling the lincomycin monoclonal antibody and magnetic bead. The invention also relates to a method for detecting lincomycin in milk and milk powder by employing the kit and a matched chemiluminescence detector, and the method has advantages of high sensitivity, specificity and short detection time for detecting lincomycin.

The invention discloses a pretreatment method for lincomycin fermentation liquor for HPLC (High Performance Liquid Chromatography) analysis. The pretreatment method comprises the steps as follows: 1) centrifugally separating the lincomycin fermentation liquor to obtain supernatant; and 2) uniformly mixing the supernatant with ethanol by volume ratio of 1:2-4, and centrifugally separating out water-soluble protein to obtain clear liquid which then can be used for HPLC analysis. After the fermentation liquor is pretreated by the pretreatment method, the impurities in samples for the HPLC analysis are greatly reduced; when the pretreatment method is used for detection of the lincomycin fermentation liquor, both lincomycin component and lincomycin B component are effectively separated from an adjacent impurity peak, the analysis effect is good, the lincomycin B component can be visually represented with data and the analysis accuracy is high; and after hundreds of samples are analyzed continuously, column pressure is not obviously increased, and the chromatographic column theoretical plate number can meet detection requirements.

The invention relates to the technical field of milk detection, and discloses a method for detecting lincomycin in milk based on background fluorescence quenching-immunochromatography. The method for detecting lincomycin in milk based on background fluorescence quenching-immunochromatography comprises the following steps: 1, inputting a lincomycin standard curve equation and a name to a two-dimensional code encoder in order to make a lincomycin two-dimensional code, and pasting the lincomycin two-dimensional code in the two-dimensional code pasting position of a lincomycin test strip in order to obtain a lincomycin detection card; and 2, taking 200 [mu]L of a sample to be detected to a small test cup fixedly containing a colloidal-gold antibody. The detection limit of lincomycin in milk n is 0.088 [mu]g / kg, the optimal sample addition volume is 100 [mu]L, and the optimal chromatographic time is 10 min. Compared with existing methods, the method disclosed in the invention has the advantages of high sensitivity, short reaction time and easiness in operation, can realize onsite rapid quantitative detection of lincomycin in the milk, and lays a foundation for the wide application to the field of food safety.

The invention provides a boiler biomass fuel. Components of the fuel are mycelium or / and mushroom dregs, as well as 0 to 80 percent of boiler fuel coal. The weight percentage of moisture contained by the boiler biomass fuel is less than or equal to 25 percent. The preparation method comprises the step of mixing the mycelium or / and the mushroom dregs with the boiler fuel coal. The invention uses the mycelium and the mushroom dregs produced while enterprises produce penicillin, streptomycin, terramycin, tetracycline, lincomycin, gentamicin and kanamycin after simple treatment as boiler fuels, can avoid environmental pollution, and effectively utilizes heat value in the mycelium and the mushroom dregs.

The invention relates to a production method capable of improving the output of lincomycin through adding glutamine. The production method is characterized in that: the glutamine is added in the fermentation process; the quantity of added glutamine is 0.04-0.06% of the weight of a culture medium; and the adding time of the glutamine is 72-96 hours. The production method has the following advantage that: the output of the lincomycin can be obviously improved through adding the glutamine which does not serve as a precursor for synthesizing the lincomycin in the fermentation process.

The invention belongs to the technical field of functional material preparation, and more specifically relates to a preparation method and applications of click chemistry based lincomycin molecularlyimprinted composite membrane. The preparation method comprises following steps: dopamine and polyethyleneimine are taken as hydrophilic modification materials, Lincomycin is taken as template molecule, 4-vinylpyridine is taken as a functional monomer, Pentaerythritol Tetra(3-mercaptopropionate) is taken as a crosslinking agent, Dipentaerythritol penta- / hexa-acrylate is taken as an auxiliary crosslinking agent, based on click chemistry polymerization method, the lincomycin molecule composite film is prepared. The prepared click chemistry based lincomycin molecularly imprinted composite membraneis capable of solving problems in the prior art that recycling of conventional lincomycin molecule imprinting polymer is difficult, and secondary pollution is easily caused; and in addition, the click chemistry based lincomycin molecularly imprinted composite membrane possesses excellent specific recognition capacity and adsorption separation capacity on Lincomycin.

The invention relates to a detection kit, in particular relates to a lincomycin chemiluminiscent enzyme-linked immunosorbent assay rapid detection kit. The rapid detection kit comprises a kit body, an enzyme plate arranged in the kit body and a reagent in the kit body, wherein all holes of the enzyme plate are coated with envelope antigens, the envelope antigens are prepared from lincomycin metabolites and ovalbumin by coupling; the reagent comprises a lincomycin monoclonal antibody, a horseradish peroxidase labeled goat-anti-mouse antibody, lincomycin-series standard solutions, a condensed phosphate buffer solution, a condensed washing solution and a luminous solution. The detection kit can be used for detecting residual lincomycin and determining the remnant of the lincomycin by adopting the steps of adding samples, washing, adding the horseradish peroxidase labeled goat-anti-mouse antibody, washing, adding the luminous solution, judging the detection results and the like.

The invention discloses a composition containing rifampicin and lipiarmycin and aims at solving the problems of hepatotoxicity and easiness in producing drug resistance due to independent use of one of two antibiotics in treating bacterial infection in a human body. The weight ratio of the lincomycin to the lipiarmycin is (10 to 1) to (1 to 10). The method disclosed by the invention comprises thefollowing characteristics: firstly, two components generate a synergistic sterilization effect, and the effects at lower dosage and lower toxicity are realized; secondly, lower antibiotic natural drugresistance frequency is obtained. The composition disclosed by the invention can be used for mycobacterium tuberculosis infection which is a disease needing long-term treatment; drug resistance is not easy to produce and toxic or side effects are reduced.

The invention discloses a method for separation of lincomycin. The method comprises the following steps of acidizing a lincomycin-containing fermentation liquor, carrying out filtration, carrying out alkalinity regulation, carrying out filtration again, collecting a filtrate, treating the filtrate as a sample liquid by a polystyrene-divinylbenzene reversed-phase adsorption resin, carrying out elution purification of the filtrate by an alkaline aqueous solution having a pH value the same as a pH value of the filtrate, eluting lincomycin by n-butanol, collecting the n-butanol solution of lincomycin, carrying out concentration, acidification and crystallization, and carrying out recrystallization by acetone. The method provided by the invention has the advantages of appropriate solvent consumption, simple processes, high product lincomycin purity more than 98%, and low lincomycin component B content less than 0.5%.

The invention discloses a method for improving lincomycin by fed-batch cultivation. The method is characterized by comprising the step of feeding glucose and ammonium sulfate to a fermentation tank in a fermentation process, so as to improve the lincomycin yield. By adopting the method disclosed by the invention, the fermentation level of the lincomycin can be greatly improved.

The invention belongs to the technical field of photoelectrochemical analysis, and particularly relates to a preparation method and application of an MOFs composite TiO2 photoactive material electrode. The preparation method comprises the following steps of S01, preparing HKUST-1, S02, dropwise adding the TiO2 dispersion liquid on an ITO electrode, S03, preparing an HKUST-1 / TiO2 / ITO modified electrode, and S04, preparing a CS / HKUST-1 / TiO2 / ITO composite electrode. The step S04 is replaced by the step S04', and the step S04' comprises the substeps that the HKUST-1 / TiO2 / ITO modified electrode obtained in the step S03 is heated to 300-380 DEG C at the speed of 8-12 DEG C / min, roasted for 0.8-1.5 h and cooled, and then the HKUST-CuO / TiO2 / ITO composite electrode is obtained. The hollow and thin-layer defect structure of HKUST-CuO is beneficial to enhancing the multiple diffuse reflection effect on visible light, a sensor platform can be further applied to high-sensitivity detection of other biomolecules such as alpha fetoprotein (AFP), lincomycin (Lin) and heavy metal ions Hg<2+>, and meanwhile, the composite material also has an obvious effect on catalytic degradation of dye molecules.

The invention relates to a method for detecting lincomycin antibiotics, in particular to a method for rapidly detecting the lincomycin antibiotics, and more particular to a method for rapidly detecting illegally-added lincomycin antibiotics in anti-acne cosmetics. The method for detecting the lincomycin antibiotics in cosmetics comprises the following steps of: adding a periodate solution, an acidic modifier and cyclohexane to a sample to be detected or sample solution to be detected; observing the color of an upper-layer solvent after 3-30 min; if the color is between pink and amaranthine, indicating that the sample to be detected contains the lincomycin antibiotics; and if a cyclohexane layer is not between pink and amaranthine, indicating that the sample to be detected contains no lincomycin antibiotics. The method provided by the invention can be used for achieving the purposes of targeted sampling and target testing and has the characteristics of simpleness, easiness for operation, rapidness in detection, accurate result, extremely low test cost and capability of rapidly screening a large number of samples.

The invention discloses a kanamycin, erythrocin and lincomycin three-in-one gold-labeled micro-hole strip. A kit comprises a sample plate at least provided with one sample groove and at least one test strip; a colloidal gold-labeled object of a kanamycin specific antibody, a colloidal gold-labeled object of an erythrocin specific antibody and a colloidal gold-labeled object of a lincomycin specific antibody are attached to the bottom of each sample groove; the test strip comprises a bottom plate (1), a reaction film (2), a sample absorbent pad (3) and an absorbent pad (4), wherein the sample absorbent pad, the reaction film and the absorbent pad are sequentially attached to the bottom plate; a line T3, a line T2, a line T1 and a line C are sequentially arranged on the reaction film from beginning to tail end, and the line T3, the line T2 and the line T1 are coated with a lincomycin-carrier protein coupling, an erythrocin-carrier protein coupling and a kanamycin-carrier protein coupling respectively; the C line is coated with an antiantibody. The kit has the wide market prospect.

The invention provides a near-infrared fluorescent microsphere immunochromatography test strip rapid detection method for common antibiotics in milk, and the method comprises the following steps: S1, preparing a PVC base plate, and a 2mm sample pad, a nitrocellulose membrane and absorbent paper which are overlapped and pasted on the base plate; treating the sample pad through a sample pad diluent, and coating the nitrocellulose membrane with an artificial antigen of antibiotics as a detection line and coated with a goat anti-rabbit antibody as a quality control line; and S2, respectively coupling the near-infrared fluorescent microspheres with a sulfanilamide monoclonal antibody, a quinolone monoclonal antibody, a lincomycin monoclonal antibody and a rabbit anti-goat antibody. According to the application, an immunochromatography test strip system can be effectively improved; the detection method has the advantages of high sensitivity, good stability and low cost, and can be used for on-site rapid determination of sulfanilamide, quinolone and lincomycin in milk.

The invention discloses a liquid chromatography-mass spectrometry method for simultaneously determining lincomycin and gentamicin in animal blood plasma. The liquid chromatography-mass spectrometry method for simultaneously determining the lincomycin and the gentamicin in the animal blood plasma, disclosed by the invention, comprises the following steps: (1) simultaneously extracting the lincomycin and the gentamicin in blood plasma to be detected by utilizing an extracting solvent, so as to obtain an extracting solution; (2) carrying out qualitative and quantitative determination on the extracting solution by adopting a high performance liquid chromatography tandem mass spectrometry. According to the liquid chromatography-mass spectrometry method disclosed by the invention, the lincomycin and the gentamicin in the blood serum can be simultaneously extracted by utilizing the same extracting reagent, so that a complicated process of extracting step by step is avoided; an ion pair reagent is not used in a sample pre-treatment process and normal utilization of an instrument is not influenced. A method for pre-treating a blood serum sample and the liquid chromatography-mass spectrometry method have the advantages of simple steps, high sensitivity and accurate and reliable result and can be used for simply and efficiently determining the lincomycin and the gentamicin in the animal blood plasma.

The invention relates to controlled-release lincomycin granules. Firstly, tamoxifen citrate is prepared into a clathrate compound and then the clathrate compound is prepared into the controlled-release granules.

Provided in the present invention are a class of Lincomycin biosynthetic intermediates and a preparation method and use thereof. Specifically provided are Lincomycin biosynthetic intermediates obtained from the genetic modification of a Lincomycin producing bacterium, and a method for the production thereof through fermentation and purification through separation.

The invention discloses a kanamycin, erythrocin and lincomycin three-in-one gold-labeled micro-hole strip. A kit comprises a sample plate at least provided with one sample groove and at least one test strip; a colloidal gold-labeled object of a kanamycin specific antibody, a colloidal gold-labeled object of an erythrocin specific antibody and a colloidal gold-labeled object of a lincomycin specific antibody are attached to the bottom of each sample groove; the test strip comprises a bottom plate (1), a reaction film (2), a sample absorbent pad (3) and an absorbent pad (4), wherein the sample absorbent pad, the reaction film and the absorbent pad are sequentially attached to the bottom plate; a line T3, a line T2, a line T1 and a line C are sequentially arranged on the reaction film from beginning to tail end, and the line T3, the line T2 and the line T1 are coated with a lincomycin-carrier protein coupling, an erythrocin-carrier protein coupling and a kanamycin-carrier protein coupling respectively; the C line is coated with an antiantibody. The kit has the wide market prospect.

The invention discloses a method for determining lincosamine antibiotics in feed. The method comprises the following steps: extracting three lincosamine antibiotics (lincomycin, clindamycin and pirithromycin) in a feed by adopting a 80% methanol dipotassium phosphate solution, and purifying a sample by adopting a mixed cation exchange (MCX) solid-phase extraction small column. The lincomycin, the clindamycin and the pirithromycin are quantified at the same time through liquid chromatography-tandem mass spectrometry (LC-MS / MS). Results show that under the addition level of 40-4000 [mu] g kg <-1 >, the average recovery rate of lincosamines in seven feeds is 81.5%-103.3%, and the relative standard deviation within the day and between the day is less than 10%. The limit of detection (LOD) and the limit of quantitation (LOQ) in the feed are between 3.6-9.8 [mu] g kg <-1 > and 11.4-31.3 [mu] g kg <-1 >. The method is suitable for simultaneously determining three antibiotics, namely lincomycin, clindamycin and pirilimycin, in the feed. The method is accurate, sensitive, simple and convenient to operate and good in stability.

The invention discloses a specific culture medium for Lactobacillus plantarum, which is convenient to configure and low in cost. The culture medium contains substances such as lincomycin and carbon source. Plantarum can be cultured and counted specifically in fermented milk, lactic acid bacteria drinks or bacteria powder; the present invention also provides an application utilizing the above-mentioned plantarum specific culture medium, which is used for plantarum lactobacillus To count viable bacteria is to culture the strains on the culture medium, and then count the cultured bacterial strains on plates. This counting method is simple and has high specificity. Compared with the PCR counting method, it is more difficult for operators. Lower requirements and lower costs. The invention is suitable for configuring a culture medium capable of specifically counting the number of viable Lactobacillus plantarum.

The invention relates to a process for producing lincomycin by fed-batch fermentation, which comprises seed culture, shake flask culture, secondary tank culture and fermentation culture in a fermentor. During the fermentation process, a small material is added which contains 8-12 parts of soybean cake powder, 15-20 parts of corn syrup, 4-5 parts of calcium carbonate, 2-3 parts of magnesium sulfate, 0.2-0.4 parts of leucine, and dimethyl silicone oil. 3-0.5 parts, soybean oil 0.3-0.5 parts and water 200 parts; ammonium sulfate solution or ammonia water is added as needed to regulate the pH of fermentation broth; the sugar solution is regulated as needed to keep the reducing sugar content in the fermentation broth in an appropriate range. The titer of lincomycin obtained by fermentation withthe process provided by the invention is more than 10000U / mL, and the content of B component in the separated and purified lincomycin is less than 0.5%.

The invention discloses a detection method for lincomycin and a special test paper therefor. The test paper includes a sample adsorption pad, a conjugate release pad, a reaction membrane, and a water absorption pad connected in order. The conjugate release pad is coated with a colloidal gold labeled lincomycin prime specific antibody, which is a lincomycin polyclonal antibody or a lincomycin monoclonal antibody. The reaction membrane comprises a detection zone and a quality control zone, wherein the detection zone is coated with a conjugate of hapten and carrier protein, and the quality control zone is coated with an antiantibody. The method for detection of lincomycin by the test paper provided by the invention is simple, fast, visualized and accurate, and has the advantages of wide application range, low cost, and easy popularization and application.

The invention discloses compound astragalus polysaccharide injection used for pigs and a preparation method thereof. Every 100L of compound astragalus polysaccharide injection used for the pigs contains the following raw materials, such as 0.5-5kg of astragalus polysaccharide, 1-10kg of lincomycin, 5-30kg of analginum, 1-10kg of inosine, 3-6L of 2-hydroxyl ethylamine and the like, wherein the astragalus polysaccharide can accelerate the lymphocyte to be converted and increased in value and accelerate the phagocytic function of macrophage; the lincomycin has strong antibiosis efficacy; the astragalus polysaccharide and the lincomycin are organically combined to perform an antiviral function, widen an antibacterial spectrum and enhance a targeted action on bacteria; the analginum has various functions of resisting bacterium, diminishing inflammation, carrying out antipyretic action, relieving pain and the like; and the inosine is favorable for restoring the function of a damaged hepaticcell. Above medicines have a reasonable formula, can simultaneously carry out symptomatic treatment and etiological treatment by aiming at diseases, has quick curative effect on mixed infection of pig virus and bacteria and treats both principal and secondary aspect of the disease, the medicine resistance of the pathogenic microorganism is lowered, the clinic symptom of a diseased pig is alleviated, and therefore the pig is quickly restored.

The invention discloses an advanced treatment method for antibiotic drug sewage, and belongs to the field of wastewater treatment. The treatment method comprises the following units of a pretreatment modification unit, a first catalytic reaction unit, a biological catalytic reaction unit, a second catalytic reaction unit and a physical precipitation unit. The method is used for treating production wastewater of antibiotics such as erythromycin thiocyanate, tetracycline, lincomycin, penicillin and the like, wherein the operation is efficient, the effluent quality is safe and stable, and virtuous cycle and green production of water resources in enterprises can be realized.

The invention especially relates to a medicine for local soaking type targeted treatment of meniscus injury through administration in knuckle articular cavity, belonging to the field of medical technology. The medicine comprises the following components: two ampoules of high-molecular sodium hyaluronate, wherein each ampoule has a volume of 2 ml and contains 25 mg of high-molecular sodium hyaluronate; two ampoules of lincomycin, wherein each ampoule has a volume of 5 ml and contains 0.6 g of lincomycin; two ampoules of dexamethasone, wherein each ampoule has a volume of 1 ml and contains 5 mg of dexamethasone; and one ampoule of normal saline, wherein the ampoule has a volume of 10 ml. The medicine provided by the invention is mainly prepared from high-molecular sodium hyaluronate, lincomycin, dexamethasone, normal saline and other drugs according to or partially according to certain composition and dosage; cooperatively used drugs are a non-ionic iodine contrast agent solution and a lidocaine local anesthetic; and the medicine can be organically combined with a variety of meniscus diagnosis and treatment means, is simple, safe, practical and efficient, has reliable curative effect and can effectively treat meniscus injury.

The invention relates to a detection kit, in particular relates to a lincomycin chemiluminiscent enzyme-linked immunosorbent assay rapid detection kit. The rapid detection kit comprises a kit body, an enzyme plate arranged in the kit body and a reagent in the kit body, wherein all holes of the enzyme plate are coated with envelope antigens, the envelope antigens are prepared from lincomycin metabolites and ovalbumin by coupling; the reagent comprises a lincomycin monoclonal antibody, a horseradish peroxidase labeled goat-anti-mouse antibody, lincomycin-series standard solutions, a condensed phosphate buffer solution, a condensed washing solution and a luminous solution. The detection kit can be used for detecting residual lincomycin and determining the remnant of the lincomycin by adopting the steps of adding samples, washing, adding the horseradish peroxidase labeled goat-anti-mouse antibody, washing, adding the luminous solution, judging the detection results and the like.

The invention discloses a lincomycin solid dispersion preparation and a preparation method thereof, and belongs to the technical field of new veterinary drug solid dispersion formulations. The lincomycin solid dispersion preparation is prepared from, by mass, 10-50% of lincomycin, 30-50% of polyvidone, 10-50% of poloxamer and 0-50% of drug auxiliary materials. The lincomycin is prepared into a solid dispersion, and by means of the hot-melt extrusion inclusion technology, a drug carrier can be molten in a short time to uniformly wrap the drug; the prepared lincomycin solid dispersion has the advantages that the unpleasant odor of the drug raw materials is masked, the oral palatability and absorbing ability of livestock and poultry are excellent, the water solubility of the drug is high, and the biological utilization rate is high.

The present invention provides an ELISA kit for detecting lincomycin comprising a coating antigen and an enzyme labeled reagent, wherein the coating antigen is selected from the group consisting of a lincomycin hapten-carrier protein conjugate, a lincomycin antibody and a lincomycin anti-antibody; when the coating antigen is the lincomycin hapten-carrier protein conjugate, the enzyme labeled reagent is an enzyme-labeled lincomycin anti-antibody; when the coating antigen is the lincomycin antibody, the enzyme labeled reagent is an enzyme-labeled lincomycin hapten-carrier protein conjugate; and when the coating antigen is the lincomycin anti-antibody, the enzyme labeled reagent is an enzyme-labeled lincomycin hapten-carrier protein conjugate; and the lincomycin hapten is obtained through the condensation reaction between lincomycin and succinic anhydride. The ELISA kit according to the present invention can be used for detecting the content of lincomycin remained in a sample such as an animal tissue (muscle, liver), honey, etc.

The invention belongs to the technical field of an suspending agent liquid used for animal and a preparation method, and concretely relates to a suspension containing lincomycin and salinomycin sodium salt and a preparation method thereof. The suspension comprises the following components: a) lincomycin and salinomycin sodium salt bulk drugs; b)a suspending agent; c)a wetting agent; d)an antiseptic and e)an antiseptic. The suspension containing lincomycin and salinomycin sodium salt is prepared by employing a dispersion method, property of the suspension is characterized in that the suspension is white and viscous. The suspension containing lincomycin and salinomycin sodium salt has the advantages of good stability, good palatability, long elimination half life and high bioavailability, and can be used as an oral preparation for animal.