33 results about "Lincomycin" patented technology

A veterinary medicine for preventing and treating the frequently encountered diseases of pig, such as metritis, mammitis, acyesis, sterility, dysentery, epidemic enteritis, paratyphoid, asthma, etc is prepared from 15 raw materials including florfenicol, amoxicillin, lucomycin, doxycycline, etc.

The invention relates to a magnetic immunochemiluminescence kit for detecting lincomycin, which comprises the following reagents, enzyme-labelled antigen, an enzyme-labelled antigen weak solution, a magnetic antibody, a magnetic antibody weak solution, a lincomycin series standard substance solution, a chemiluminescence substrate solution A, a chemiluminescence substrate solution B, a concentrated compound solution, and a concentrated washing liquid. enzyme-labelled antigen is a horseradish peroxidase-labelled lincomycin hapten marker, lincomycin hapten is keto lincomycin obtained by performing pyridinium dichromate cas oxidation on alcoholic hydroxyl group from lincomycin, keto lincomycin and aminobutyric acid are subjected to a condensation reaction to obtain four-carbon chain carboxyl hapten, the magnetic antibody is obtained by coupling the lincomycin monoclonal antibody and magnetic bead. The invention also relates to a method for detecting lincomycin in milk and milk powder by employing the kit and a matched chemiluminescence detector, and the method has advantages of high sensitivity, specificity and short detection time for detecting lincomycin.

The invention discloses a pretreatment method for lincomycin fermentation liquor for HPLC (High Performance Liquid Chromatography) analysis. The pretreatment method comprises the steps as follows: 1) centrifugally separating the lincomycin fermentation liquor to obtain supernatant; and 2) uniformly mixing the supernatant with ethanol by volume ratio of 1:2-4, and centrifugally separating out water-soluble protein to obtain clear liquid which then can be used for HPLC analysis. After the fermentation liquor is pretreated by the pretreatment method, the impurities in samples for the HPLC analysis are greatly reduced; when the pretreatment method is used for detection of the lincomycin fermentation liquor, both lincomycin component and lincomycin B component are effectively separated from an adjacent impurity peak, the analysis effect is good, the lincomycin B component can be visually represented with data and the analysis accuracy is high; and after hundreds of samples are analyzed continuously, column pressure is not obviously increased, and the chromatographic column theoretical plate number can meet detection requirements.

The invention relates to the technical field of milk detection, and discloses a method for detecting lincomycin in milk based on background fluorescence quenching-immunochromatography. The method for detecting lincomycin in milk based on background fluorescence quenching-immunochromatography comprises the following steps: 1, inputting a lincomycin standard curve equation and a name to a two-dimensional code encoder in order to make a lincomycin two-dimensional code, and pasting the lincomycin two-dimensional code in the two-dimensional code pasting position of a lincomycin test strip in order to obtain a lincomycin detection card; and 2, taking 200 [mu]L of a sample to be detected to a small test cup fixedly containing a colloidal-gold antibody. The detection limit of lincomycin in milk n is 0.088 [mu]g/kg, the optimal sample addition volume is 100 [mu]L, and the optimal chromatographic time is 10 min. Compared with existing methods, the method disclosed in the invention has the advantages of high sensitivity, short reaction time and easiness in operation, can realize onsite rapid quantitative detection of lincomycin in the milk, and lays a foundation for the wide application to the field of food safety.

The invention provides a boiler biomass fuel. Components of the fuel are mycelium or/and mushroom dregs, as well as 0 to 80 percent of boiler fuel coal. The weight percentage of moisture contained by the boiler biomass fuel is less than or equal to 25 percent. The preparation method comprises the step of mixing the mycelium or/and the mushroom dregs with the boiler fuel coal. The invention uses the mycelium and the mushroom dregs produced while enterprises produce penicillin, streptomycin, terramycin, tetracycline, lincomycin, gentamicin and kanamycin after simple treatment as boiler fuels, can avoid environmental pollution, and effectively utilizes heat value in the mycelium and the mushroom dregs.

The invention belongs to the technical field of functional material preparation, and more specifically relates to a preparation method and applications of click chemistry based lincomycin molecularlyimprinted composite membrane. The preparation method comprises following steps: dopamine and polyethyleneimine are taken as hydrophilic modification materials, Lincomycin is taken as template molecule, 4-vinylpyridine is taken as a functional monomer, Pentaerythritol Tetra(3-mercaptopropionate) is taken as a crosslinking agent, Dipentaerythritol penta-/hexa-acrylate is taken as an auxiliary crosslinking agent, based on click chemistry polymerization method, the lincomycin molecule composite film is prepared. The prepared click chemistry based lincomycin molecularly imprinted composite membraneis capable of solving problems in the prior art that recycling of conventional lincomycin molecule imprinting polymer is difficult, and secondary pollution is easily caused; and in addition, the click chemistry based lincomycin molecularly imprinted composite membrane possesses excellent specific recognition capacity and adsorption separation capacity on Lincomycin.

The invention relates to a detection kit, in particular relates to a lincomycin chemiluminiscent enzyme-linked immunosorbent assay rapid detection kit. The rapid detection kit comprises a kit body, an enzyme plate arranged in the kit body and a reagent in the kit body, wherein all holes of the enzyme plate are coated with envelope antigens, the envelope antigens are prepared from lincomycin metabolites and ovalbumin by coupling; the reagent comprises a lincomycin monoclonal antibody, a horseradish peroxidase labeled goat-anti-mouse antibody, lincomycin-series standard solutions, a condensed phosphate buffer solution, a condensed washing solution and a luminous solution. The detection kit can be used for detecting residual lincomycin and determining the remnant of the lincomycin by adopting the steps of adding samples, washing, adding the horseradish peroxidase labeled goat-anti-mouse antibody, washing, adding the luminous solution, judging the detection results and the like.

The invention discloses a method for improving lincomycin by fed-batch cultivation. The method is characterized by comprising the step of feeding glucose and ammonium sulfate to a fermentation tank in a fermentation process, so as to improve the lincomycin yield. By adopting the method disclosed by the invention, the fermentation level of the lincomycin can be greatly improved.

The invention belongs to the technical field of photoelectrochemical analysis, and particularly relates to a preparation method and application of an MOFs composite TiO2 photoactive material electrode. The preparation method comprises the following steps of S01, preparing HKUST-1, S02, dropwise adding the TiO2 dispersion liquid on an ITO electrode, S03, preparing an HKUST-1/TiO2/ITO modified electrode, and S04, preparing a CS/HKUST-1/TiO2/ITO composite electrode. The step S04 is replaced by the step S04', and the step S04' comprises the substeps that the HKUST-1/TiO2/ITO modified electrode obtained in the step S03 is heated to 300-380 DEG C at the speed of 8-12 DEG C/min, roasted for 0.8-1.5 h and cooled, and then the HKUST-CuO/TiO2/ITO composite electrode is obtained. The hollow and thin-layer defect structure of HKUST-CuO is beneficial to enhancing the multiple diffuse reflection effect on visible light, a sensor platform can be further applied to high-sensitivity detection of other biomolecules such as alpha fetoprotein (AFP), lincomycin (Lin) and heavy metal ions Hg<2+>, and meanwhile, the composite material also has an obvious effect on catalytic degradation of dye molecules.

The invention relates to a method for detecting lincomycin antibiotics, in particular to a method for rapidly detecting the lincomycin antibiotics, and more particular to a method for rapidly detecting illegally-added lincomycin antibiotics in anti-acne cosmetics. The method for detecting the lincomycin antibiotics in cosmetics comprises the following steps of: adding a periodate solution, an acidic modifier and cyclohexane to a sample to be detected or sample solution to be detected; observing the color of an upper-layer solvent after 3-30 min; if the color is between pink and amaranthine, indicating that the sample to be detected contains the lincomycin antibiotics; and if a cyclohexane layer is not between pink and amaranthine, indicating that the sample to be detected contains no lincomycin antibiotics. The method provided by the invention can be used for achieving the purposes of targeted sampling and target testing and has the characteristics of simpleness, easiness for operation, rapidness in detection, accurate result, extremely low test cost and capability of rapidly screening a large number of samples.

The invention discloses a method for determining lincosamine antibiotics in feed. The method comprises the following steps: extracting three lincosamine antibiotics (lincomycin, clindamycin and pirithromycin) in a feed by adopting a 80% methanol dipotassium phosphate solution, and purifying a sample by adopting a mixed cation exchange (MCX) solid-phase extraction small column. The lincomycin, the clindamycin and the pirithromycin are quantified at the same time through liquid chromatography-tandem mass spectrometry (LC-MS/MS). Results show that under the addition level of 40-4000 [mu] g kg <-1 >, the average recovery rate of lincosamines in seven feeds is 81.5%-103.3%, and the relative standard deviation within the day and between the day is less than 10%. The limit of detection (LOD) and the limit of quantitation (LOQ) in the feed are between 3.6-9.8 [mu] g kg <-1 > and 11.4-31.3 [mu] g kg <-1 >. The method is suitable for simultaneously determining three antibiotics, namely lincomycin, clindamycin and pirilimycin, in the feed. The method is accurate, sensitive, simple and convenient to operate and good in stability.

The invention relates to a process for producing lincomycin by fed-batch fermentation, which comprises seed culture, shake flask culture, secondary tank culture and fermentation culture in a fermentor. During the fermentation process, a small material is added which contains 8-12 parts of soybean cake powder, 15-20 parts of corn syrup, 4-5 parts of calcium carbonate, 2-3 parts of magnesium sulfate, 0.2-0.4 parts of leucine, and dimethyl silicone oil. 3-0.5 parts, soybean oil 0.3-0.5 parts and water 200 parts; ammonium sulfate solution or ammonia water is added as needed to regulate the pH of fermentation broth; the sugar solution is regulated as needed to keep the reducing sugar content in the fermentation broth in an appropriate range. The titer of lincomycin obtained by fermentation withthe process provided by the invention is more than 10000U/mL, and the content of B component in the separated and purified lincomycin is less than 0.5%.

The invention discloses an advanced treatment method for antibiotic drug sewage, and belongs to the field of wastewater treatment. The treatment method comprises the following units of a pretreatment modification unit, a first catalytic reaction unit, a biological catalytic reaction unit, a second catalytic reaction unit and a physical precipitation unit. The method is used for treating production wastewater of antibiotics such as erythromycin thiocyanate, tetracycline, lincomycin, penicillin and the like, wherein the operation is efficient, the effluent quality is safe and stable, and virtuous cycle and green production of water resources in enterprises can be realized.

The invention especially relates to a medicine for local soaking type targeted treatment of meniscus injury through administration in knuckle articular cavity, belonging to the field of medical technology. The medicine comprises the following components: two ampoules of high-molecular sodium hyaluronate, wherein each ampoule has a volume of 2 ml and contains 25 mg of high-molecular sodium hyaluronate; two ampoules of lincomycin, wherein each ampoule has a volume of 5 ml and contains 0.6 g of lincomycin; two ampoules of dexamethasone, wherein each ampoule has a volume of 1 ml and contains 5 mg of dexamethasone; and one ampoule of normal saline, wherein the ampoule has a volume of 10 ml. The medicine provided by the invention is mainly prepared from high-molecular sodium hyaluronate, lincomycin, dexamethasone, normal saline and other drugs according to or partially according to certain composition and dosage; cooperatively used drugs are a non-ionic iodine contrast agent solution and a lidocaine local anesthetic; and the medicine can be organically combined with a variety of meniscus diagnosis and treatment means, is simple, safe, practical and efficient, has reliable curative effect and can effectively treat meniscus injury.

The invention discloses a lincomycin solid dispersion preparation and a preparation method thereof, and belongs to the technical field of new veterinary drug solid dispersion formulations. The lincomycin solid dispersion preparation is prepared from, by mass, 10-50% of lincomycin, 30-50% of polyvidone, 10-50% of poloxamer and 0-50% of drug auxiliary materials. The lincomycin is prepared into a solid dispersion, and by means of the hot-melt extrusion inclusion technology, a drug carrier can be molten in a short time to uniformly wrap the drug; the prepared lincomycin solid dispersion has the advantages that the unpleasant odor of the drug raw materials is masked, the oral palatability and absorbing ability of livestock and poultry are excellent, the water solubility of the drug is high, and the biological utilization rate is high.

The invention relates to a detection gel column of lincomycin and a detection method of lincomycin. The detection gel column of lincomycin comprises an open tubular column as well as a detection layer and a quality control layer which are filled in the open tubular column. During detection, a sample to be measured and a lincomycin enzyme-labelled antigen labelled with a chemiluminescent label are added into the detection gel column of lincomycin and flow through the detection layer and the quality control layer. The sample to be measured and the lincomycin enzyme-labelled antigen labelled with the chemiluminescent label together compete for a lincomycin monoclonal antibody in the detection layer, and color of the detection layer and color of the quality control layer are used for judging whether the sample to be measured contains lincomycin. The detection gel column of lincomycin has carrying convenience, and can be used for rapidly and sensitively detecting whether the sample to be measured contains lincomycin, and the method is suitable for rapid on-site detection of a lot of samples.