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Method for detecting lincomycin in milk based on background fluorescence quenching-immunochromatography

A lincomycin and fluorescence quenching technology, applied in measurement devices, analytical materials, biological tests, etc., can solve problems such as poor reproducibility, cumbersome processing, and inability to perform quantitative analysis, and achieve short reaction time and high sensitivity. , the effect of easy operation

Inactive Publication Date: 2017-08-11
THE CENT FOR DISEASE CONTROL & PREVENTION OF XINJIANG UYGUR AUTONOMOUS REGION
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention provides a method for determining lincomycin in milk based on background fluorescence quenching-immunochromatography, which overcomes the deficiencies of the prior art and can effectively solve the existing detection of lincomycin residues in fresh milk The method has the problems of cumbersome sample pretreatment process, poor reproducibility, not suitable for on-site rapid detection and quantitative analysis

Method used

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  • Method for detecting lincomycin in milk based on background fluorescence quenching-immunochromatography
  • Method for detecting lincomycin in milk based on background fluorescence quenching-immunochromatography
  • Method for detecting lincomycin in milk based on background fluorescence quenching-immunochromatography

Examples

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Embodiment 1

[0015] Example 1, the method for determining lincomycin in milk based on background fluorescence quenching-immunochromatography is carried out according to the following steps: the first step is to enter the lincomycin standard curve equation and name into the two-dimensional code system Make a lincomycin two-dimensional code, and then paste the lincomycin two-dimensional code on the two-dimensional code on the lincomycin test strip to get the lincomycin test card; the second step, Take 200 μL of the sample to be tested into a small test cup immobilized with gold-labeled antibody, mix well, pipette 100 μL dropwise into the sample hole on the lincomycin test card, and perform chromatography at room temperature for 10 minutes; the third step is to layer After the analysis, insert the lincomycin detection card into the card slot of the fluorescent immunoassay analyzer, click the test sample, and the display screen of the fluorescent immunoassayer will display the name of the sampl...

Embodiment 2

[0016] Example 2, the lincomycin standard curve equation is obtained by the following method: the first step, the configuration of lincomycin stock solution, take lincomycin standard substance, and use PBS to make 1 μg / mL lincomycin Solution, to obtain lincomycin stock solution; the second step, the configuration of lincomycin series concentration standard solution, the lincomycin stock solution is sequentially made into 10ng / mL, 20ng / mL, 30ng / mL with standard milk samples , 40ng / mL, and 60ng / mL lincomycin series solutions to obtain lincomycin series concentration standard solutions; in the third step, take 200 μL of standard milk samples and 200 μL of lincomycin series concentration standard solutions until gold is immobilized. In the small test cup labeled with the antibody, draw 100 μL dropwise into the sample hole on the lincomycin test strip after mixing well, and perform chromatography at room temperature for 10 minutes; the fourth step is to test the lincomycin Insert t...

Embodiment 3

[0017] Example 3, as an optimization of the above example, the standard milk sample is a milk sample that does not contain lincomycin after testing.

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Abstract

The invention relates to the technical field of milk detection, and discloses a method for detecting lincomycin in milk based on background fluorescence quenching-immunochromatography. The method for detecting lincomycin in milk based on background fluorescence quenching-immunochromatography comprises the following steps: 1, inputting a lincomycin standard curve equation and a name to a two-dimensional code encoder in order to make a lincomycin two-dimensional code, and pasting the lincomycin two-dimensional code in the two-dimensional code pasting position of a lincomycin test strip in order to obtain a lincomycin detection card; and 2, taking 200 [mu]L of a sample to be detected to a small test cup fixedly containing a colloidal-gold antibody. The detection limit of lincomycin in milk n is 0.088 [mu]g / kg, the optimal sample addition volume is 100 [mu]L, and the optimal chromatographic time is 10 min. Compared with existing methods, the method disclosed in the invention has the advantages of high sensitivity, short reaction time and easiness in operation, can realize onsite rapid quantitative detection of lincomycin in the milk, and lays a foundation for the wide application to the field of food safety.

Description

technical field [0001] The invention relates to the technical field of milk detection, and relates to a method for detecting lincomycin in milk based on background fluorescence quenching-immunochromatography. Background technique [0002] Lincomycin (Linconycin, LIN) belongs to the lincosamide antibiotics, which has strong antibacterial effect on Gram-positive bacteria and antibacterial effect on Mycoplasma, but is ineffective against Gram-negative bacteria. Clinically, it is mainly used in respiratory tract infection caused by staphylococcus, streptococcus pyogenes, pneumococcus and anaerobic bacteria, skin and soft tissue infection, female reproductive tract and pelvic infection, and abdominal infection caused by anaerobic bacteria. It can also be used for septicemia caused by streptococcus and staphylococcus, bone and joint infection, surgical adjuvant treatment of chronic bone and joint infection, acute hematogenous osteomyelitis caused by staphylococcus, etc. As a vete...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/58
CPCG01N33/9446G01N33/582
Inventor 王玉文吴晓霞李新霞李久彤张唯徐莉华
Owner THE CENT FOR DISEASE CONTROL & PREVENTION OF XINJIANG UYGUR AUTONOMOUS REGION
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