The invention discloses conditioning paste for treating cat abdominal infection and a preparation method of the conditioning paste. The conditioning paste is prepared from the following components: Chinese herbaceous peony, raw liquorice, codonopsis pilosula, bighead atractylodes rhizome, pericarpium citri reticulatae, cortex moutan, salvia miltiorrhiza, red paeony root, radix bupleuri, patrinia, selfheal, dandelion, Chinese waxgourd seed, honeysuckle, radix stemonae and ginger. The feline infectious peritonitis is a chronic, progressive and lethal infectious disease caused by feline infectious peritonitis virus, has the main characteristics of peritonitis, accumulation of a large amount of ascites and high fatality rate, and has the external symptoms of stomachache, nausea and vomiting, fever, unclear recognition and the like. According to the invention, the Chinese herbaceous peony and the raw licorice are used as monarch drugs for nourishing liver, relieving spasm and pain, clearing heat and detoxifying. Codonopsis pilosula, atractylodes macrocephala koidz and pericarpium citri reticulatae are adopted to promote transportation and transformation of spleen and stomach of pets and enhance autoimmunity, radix stemonae and ginger are adopted to kill insects and bacteria, and the death rate of infected cats on the abdomen is reduced by adopting prevention and conditioning principles.