38 results about "Feline coronavirus" patented technology

The invention relates to a nucleoside compound and application of the nucleoside compound in treatment of feline infectious peritonitis. The compound belongs to a GS-441524 analogue. The compound has a very small toxic effect on normal cells of cats, and has an obvious inhibition effect on feline coronavirus. The compound can be used for preparing a feline coronavirus inhibitor, is used for treating feline infectious peritonitis, has the advantages of good curative effect, high safety and the like, and is very good in water solubility and easier to absorb.

Provided are methods of treating feline Coronavirus infections using carbanucleoside compounds having a 1′-(4-aminopyrrolo[2,1-f][1,2,4]triazin-7-yl) substituent, or a pharmaceutically acceptable salt thereof. An exemplary compound is (2R,3R,4S,5R)-2-(4-aminopyrrolo[2,1-f][1,2,4]triazin-7-yl)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-carbonitrile.

The invention relates to a pyrrolotriazine compound, a composition and an application thereof, the pyrrolotriazine compound has a structure as shown in a formula (I), and the definitions of R1-R11 areas defined in claim 1. The pyrrolotriazine compound has an excellent antiviral effect, and particularly can be used for preparing drugs for treating feline coronavirus infection-resistant diseases, such as feline infectious peritonitis.

Novel glycopeptide antibiotic derivatives, processes for their preparation, their use as a medicine, their use to treat or prevent viral infections and their use to manufacture a medicine to treat or prevent viral infections are provided. The present invention relates to the use of glycopeptide antibiotics and their semisynthetic derivatives to treat or prevent viral infections and their use to manufacture a medicine to treat or prevent viral infections of subjects, more in particular infections with viruses belonging to Retroviridae, Herpes viridae, Flaviviridae and the Coronaviridae, like HIV (human immunodeficiency virus), HCV (hepatitis C virus), BVDV (bovine viral diarrhoea virus), SARS (severe acute respiratory syndrome) causing virus, FCV (feline coronavirus), HSV (herpes simplex virus), VZV (varicella zoster virus) and CMV (cytomegalovirus).

Novel glycopeptide antibiotic derivatives, processes for their preparation, their use as a medicine, their use to treat or prevent viral infections and their use to manufacture a medicine to treat or prevent viral infections are provided. The present invention relates to the use of glycopeptide antibiotics and their semisynthetic derivatives to treat or prevent viral infections and their use to manufacture a medicine to treat or prevent viral infections of subjects, more in particular infections with viruses belonging to Retroviridae, Herpes viridae, Flaviviridae and the Coronaviridae, like HIV (human immunodeficiency virus), HCV (hepatitis C virus), BVDV (bovine viral diarrhoea virus), SARS (severe acute respiratory syndrome) causing virus, FCV (feline coronavirus), HSV (herpes simplex virus), VZV (varicella zoster virus) and CMV (cytomegalovirus).

The invention relates to a feline coronavirus fluorescence EMA detection primer group, a kit and a detection method. The feline coronavirus fluorescence EMA detection primer group comprises an upstream primer, a downstream primer and a probe, the kit comprises a reconstitution fluid, a lyophilized enzyme tube containing primers, a positive quality control material and a negative quality control material. The detection method comprises the steps that the kit is used for performing a normal-temperature nucleic acid amplification technology, through the multiple enzymatic reactions of M-MLV reverse transcriptase, Rnase inhibitor, DNA helicase, single stranded DNA binding protein, DNA polymerase and the like, to-be-detected DNA is reversely transcribed to cDNA within 10-30 minutes under the constant temperature of 37-45 DEG C, the cDNA is amplified by millions of times, and rapid verification of the to-be-detected DNA can be achieved by cooperating with a fluorescence detection technology.The feline coronavirus fluorescence EMA detection primer group, the kit and the detection method have the advantages that the operation is simple, the time is short, the requirements for an instrument are low, and the feline coronavirus fluorescence EMA detection primer group, the kit and the detection method are suitable for the rapid diagnosis for pet infectious diseases.

The invention provides a feline coronavirus S recombinant protein, the amino acid sequence of which is shown as SEQ ID NO: 1. The invention also provides a preparation method of the feline coronavirus S recombinant protein, which comprises the following steps: (a) carrying out PCR (Polymerase Chain Reaction) amplification by taking the gene of the feline coronavirus S protein as a template, and cloning an S recombinant protein fragment; (b) carrying out electrophoretic separation on the S recombinant protein fragment, recovering and purifying, connecting to a vector, and tranforming to competent cells; (c) ligating the plasmid with the prokaryotic expression vector to obtain the recombinant plasmid. and (d) transforming the recombinant plasmid to BL21 for prokaryotic expression to obtain the feline coronavirus S recombinant protein. The inventor finds that the protein fragment in the FCoV S2 protein has good reactogenicity and immunogenicity for the first time, and can be used as a basis for subsequent research and development of FCoV vaccines and related diagnosis and detection kits.

The invention discloses a cat coronavirus integrated nucleic acid detection card box. The cat coronavirus integrated nucleic acid detection card box comprises a box body, wherein a cracking cavity, a first cleaning cavity and a second cleaning cavity are sequentially arranged in the box body from top to bottom, the cracking cavity communicates with a box cover, and the second cleaning cavity communicates with a reaction cavity arranged at the bottom of the outer side of the box body; plungers with plunger hole directions perpendicular to a connecting direction are respectively arranged in every two connecting intervals of the cracking cavity, the first cleaning cavity, the second cleaning cavity and the reaction cavity, and hydrophobic liquid sealing materials are arranged in plunger holes of the plungers; amplification reaction liquid is arranged in the reaction cavity and comprises primer probe mixed liquid of a cat coronavirus ORF gene; and a soluble material layer is arranged in the reaction cavity, and a DNA polymerase solution is embedded in the soluble material layer. The cat coronavirus integrated nucleic acid detection card box disclosed by the invention can quickly and integrally perform PCR amplification and detection on cat coronavirus under the condition of relatively low cost, and solves the problems of poor repeatability, time consumption of a detection method, complicated operation and the like in the prior art.

The invention provides an indirect ELISA kit for detecting a feline coronavirus antibody. The kit contains a coating antigen S recombinant protein; the amino acid sequence of the S recombinant protein is as shown in SEQ ID NO: 1. The invention also provides application of the kit in detection of the feline coronavirus antibody. According to the indirect ELISA kit for detecting the feline coronavirus antibody of the invention, the ELISA detection kit for the feline coronavirus antibody is developed on the basis of an indirect ELISA principle. A conservative feline coronavirus S2 protein with strong antigenicity is screened out to amplify and clone a gene; a prokaryotic expression vector is constructed, namelya prokaryotic expression fusion protein; immunogenicity analysis is carried out through Western blotting, the S recombinant protein is purified into a coating antigen; and finally the indirect ELISA kit with relatively high sensitivity, strong specificity and good stability can be obtained.

The invention provides methods and means for distinguishing FECV and FIPV, and methods and means for determining whether FIPV is present in a sample. Further provided are primers and probes for detecting FIPV specific nucleic acid sequences encoding a spike protein, antibodies for detecting a FIPV, and an immunogenic composition and use thereof for eliciting an immune response against a feline coronavirus, preferably a FIPV.

The invention discloses a feline coronavirus recombinant antigen as well as a genetic engineering subunit vaccine and the application thereof. The feline coronavirus recombinant antigen comprises a recombinant protein with a sequence as shown in SEQ ID NO: 2 or an increased or truncated sequence of the sequence as shown in SEQ ID NO: 2. The vaccine comprises the recombinant protein and a pharmaceutically acceptable carrier. The feline coronavirus recombinant antigen provided by the invention is high in immunogenicity, can generate a high-concentration neutralizing antibody, and does not generate the ADE effect. Meanwhile, the recombinant antigen of the feline coronavirus is expressed by adopting a baculovirus insect cell expression system and using a suspension culture mode of Sf9 cells, so that the expression level is high, the protein immunogenicity is good, and the vaccine prepared from the recombinant antigen has the advantages of easiness in quality control, stability among batches, low production cost and the like.

The invention relates to a cyclic carbonate nucleoside compound as shown in a formula (I), pharmaceutically acceptable salts, crystalline hydrates, solvates or tautomers of the cyclic carbonate nucleoside compound, and also discloses a pharmaceutical composition containing the cyclic carbonate nucleoside compound and application of the cyclic carbonate nucleoside compound. The compound and the pharmaceutical composition can be used for treating or preventing virus infection, such as infection of related viruses such as coronavirus, influenza virus, respiratory syncytial virus, flaviviridae virus, filoviridae virus, novel coronavirus and / or feline coronavirus. (I).

The invention discloses application of FHPI for preparing a medicine for treating feline infectious peritonitis. FHPI has the little toxic effect on normal feline cells, has the obvious inhibition effect on feline coronaviruses, and can obviously lower the virulence of viruses in cells; indirect immunofluorescence light and Western Blot determination results show that FHPI can inhibit proliferation of feline coronaviruses in cells, and has the dose dependence. Accordingly, the FHPI has the activity for resisting feline coronaviruses, can be used for preparing a feline coronavirus inhibitor, can be used for treating feline infectious peritonitis and has the advantages of being good in curative effect, high in safety and the like.

The invention provides an RT-RPA primer pair, a probe, a kit and a detection method for detecting cat coronavirus, and belongs to the technical field of molecular biological detection. Aiming at an MPgene sequence of a cat coronavirus feline infectious peritonitis viruses (Fcov), a specific primer pair and a probe are designed, and a real-time fluorescence RPA detection method is established through repeated tests. The method disclosed by the invention is strong in amplification specificity and high in sensitivity, and can be used for detecting two viruses of FCoV-I and FCoV-II serotypes which both can cause feline infectious peritonitis (FIP) in one detection test, so that the method is more efficient. Technical reference is provided for rapid diagnosis and prevention and control of Fcov.

Provided is a method for determining whether a feline is infected with pathogenic Feline Infectious Peritonitis Virus (FIPV) or Feline Enteric Infection Virus (FECV). The method involves determining the presence or absence of intact or mutated S1 / S2 and S2′ cleavage sites in the spike protein of serotype 1 feline coronaviruses (FCoV1). The presence of both intact cleavage sites is indicative of FECV. The presence of a mutation in one or both cleavage sites is indicative of FIPV. The absence of both sites is indicative of an absence of FCoV1 infection. Compositions for use in determining infection and kits are also provided.

The invention provides an HRM primer pair, kit and method for rapidly identifying feline enteric coronavirus and feline infectious peritonitis viruses, and belongs to the technical field of molecularbiological detection. The high-resolution melting (HRM) primer pair disclosed by the invention is designed according to a sequence between 29000bp and 29500bp of a full-length gene of feline coronavirus (FCOV). The feline enteric coronavirus (FECV) and the feline infectious peritonitis viruses (FIPV) can be identified and distinguished at the same time through melting curves with different shapesby one-time reaction, and technical support is provided for early diagnosis and prevention and control of diseases.

The invention relates to a nucleic acid combination product, a detection kit and a micro-fluidic chip. The nucleic acid combination product comprises at least two of the following detection primer pairs: a feline parvovirus primer pair, a feline coronavirus primer pair, a feline herpesvirus type I primer pair, a feline calicivirus primer pair, a feline leukemia virus primer pair, a feline immunodeficiency virus primer pair, a feline astrovirus primer pair, a feline rotavirus primer pair, a cat vaccinia virus primer pair and a cat reovirus primer pair. The nucleic acid combination product can be used for detecting at least two feline pathogenic viruses in the sample to be detected at one time, and is good in specificity and relatively high in detection sensitivity.

The invention relates to a test strip for detecting feline coronavirus and a kit prepared from the test strip, the test strip comprises a bottom plate, and a sample pad layer, a colloidal gold pad layer, a nitrocellulose membrane layer and a water absorption layer are sequentially lapped on the bottom plate from left to right; the colloidal gold pad layer is coated with a colloidal gold particle labeled feline coronavirus monoclonal antibody and a feline infectious peritonitis virus polyclonal antibody; the nitrocellulose membrane layer is coated with a feline coronavirus monoclonal antibody as a T1 detection line, a feline infectious peritonitis virus polyclonal antibody as a T2 detection line and goat anti-chicken IgG as a quality control line. The feline coronavirus antigen is detectedin the form of the test strip and the kit, the operation is simple, real POCT detection is achieved, and the virus transmission is effectively controlled. In addition, due to the polyantibody labelingand polyantibody coating form in the test strip, the reagent sensitivity is improved to the maximum extent, and the detection rate is increased.

The invention relates to the technical field of molecular biomedicine, in particular to a PCR (Polymerase Chain Reaction) detection kit for cat and / or dog pathogens, a detection method and application. The kit is used for detecting canine distemper virus, canine influenza A virus, canine parainfluenza virus, canine parvovirus, canine coronavirus, canine rotavirus, canine babesia, canine ascaris, canine Ehrlichia, canine brucella, rabies virus, borrelia burgdorferi, reference gene ACTB, feline herpes virus, feline calicivirus and feline parvovirus. The kit comprises primers and probes of feline coronavirus, feline immunodeficiency virus, feline leukemia virus, feline mycoplasma, feline mycoplasma, feline chlamydia, giardia, toxoplasma, bartonella and reference gene GAPDH, collected DNA and RNA are added into the kit, a real-time fluorescence PCR instrument is adopted for PCR reaction, FAM, HEX, ROX and CY5 fluorescence signals are collected in each cycle, analysis of related pathogens is carried out, and the kit can be used for detecting the feline and the canine. Compared with a traditional detection method, the method has the advantages of higher specificity and higher sensitivity.

Provided is a method for treatment and / or prophylaxis of FIP (feline infectious peritonitis) in cats. The method is performed by administering to a cat a composition comprising a therapeutic and / or prophylactic amount of a cysteine protease inhibitor. The cysteine protease inhibitor can be a selective cathepsin B inhibitor. Also provided is a method for inhibiting FIPV (Feline infectious peritonitis virus) replication. The method comprises contacting a cell infected with FIPV with a cysteine protease inhibitor in an amount effective to inhibit replication of FIPV. It is expected the method will be effective for therapy or prophylaxis of FIP in any species of cat and will be effective against any strain of feline coronavirus.

The invention relates to a cat coronavirus gene amplification primer and a genotyping method. The primer is a cat coronavirus S gene primer, and a high-resolution melting curve (HRM) identification method of an SNP site (g.23531A>Y) on a cat coronavirus fibrin gene S can be established by applying the primer. A detection method established by applying the primer can be used for rapidly detecting and identifying the genotype of the 23531st site of the cat coronavirus genome from a pet clinical sample; and thus, and then the systemic infectivity phenotype of the corresponding strain can be deduced, so that the primer can be used as a basis for screening pathogenicity of the cat coronavirus, and can also be used as an important reference for clinical diagnosis of infectious peritonitis of cats.

The invention belongs to the technical field of RNA vaccines, and particularly relates to an RNA vaccine for feline coronavirus and a construction method of the RNA vaccine. The RNA vaccine is synthesized by adopting a sequence containing 5' UTR (Untranslated Region), S protein, a termination codon, 3' UTR and polyA nucleic acid; the S protein is selected from an FCoV-I type or an FCoV-II type; and the sequences of the 5' UTR and the 3' UTR are selected from feline hemoglobin or feline albumin of a feline source, and the homology of the selected sequences is 50%-100%. According to the RNA vaccine, cellular immunity and humoral immunity can be induced at the same time, cell-free production can be achieved, toxic chemicals do not need to be introduced in the production process, virus variation can be efficiently and rapidly treated, compared with a traditional vaccine, the RNA vaccine for the feline coronavirus can effectively shorten the vaccine research and development and production cycle, and an effective technical means is provided for preventing and controlling the outbreak of the FCoV.

The invention belongs to the technical field of medicines, and particularly relates to application of a bromophenol-pyrazoline compound in treatment of feline coronavirus diseases. The research finds that the bromophenol-pyrazoline compound can inhibit the activity of feline infectious peritonitis virus main protease (FIPV Mpro) and interfere replication of Feline Infectious Peritonitis Virus (FIPV) in cells. In clinical experiments, the bromophenol-pyrazoline compound can treat feline infectious peritonitis naturally infected by the FIPV, greatly improves the survival rate of sick cats, and can be used for preparing medicines for treating the feline infectious peritonitis.

The invention discloses primers and a kit for detecting cat intestinal infection pathogens and application. The primers comprise two primer groups: a Feline Picornavirus (FPV) primer group and a Feline coronavirus (FCoV) primer group, each of the two primer groups consists of six primers: a primer F3, a primer B3, a primer FIP, a primer BIP, a primer LF and a primer LB, and the sequences are different according to pathogens to which the primers aim. The detection kit comprises an FPV detection reagent, an FCoV detection reagent, a negative control, a pretreatment solution A and a pretreatmentsolution B. The use method of the kit comprises the following steps: dipping an infectious cat sample, treating the sample, putting the sample into the detection reagents, performing reaction at a constant temperature of 65 DEG C, and judging the yin-yang property according to the color change. The application is based on an LAMP technology, specifically amplifies pathogen gene segments, can finish sampling and detection within 60 minutes, and realizes high-precision detection results by using an operation mode which is most easy to master.

Provided is a method for determining whether a feline is infected with pathogenic Feline Infectious Peritonitis Virus (FIPV) or Feline Enteric Infection Virus (FECV). The method involves determining the presence or absence of intact or mutated S1 / S2 and S2′ cleavage sites in the spike protein of serotype 1 feline coronaviruses (FCoV1). The presence of both intact cleavage sites is indicative of FECV. The presence of a mutation in one or both cleavage sites is indicative of FIPV. The absence of both sites is indicative of an absence of FCoV1 infection. Compositions for use in determining infection and kits are also provided.

The present invention provides methods and tools for distinguishing FECV from FIPV, and methods and tools for determining whether FIPV is present in a sample. The present invention further provides primers and probes for detecting FIPV-specific nucleic acid sequences encoding spike proteins, antibodies for detecting FIPV, and immunogenic compositions for stimulating immune responses against feline coronaviruses, preferably FIPV. and its applications.

The invention discloses a nucleic acid composition, a kit and a detection method for detecting infectious pathogens of a cat digestive tract, and relates to the technical field of in-vitro diagnosis of molecular biotechnology. The invention provides the nucleic acid composition and the kit for detecting the infectious pathogens of the cat digestive tract. The nucleic acid composition and the kit thereof can realize rapid and accurate detection of cat leukopenia virus and cat coronavirus at most, which is of great significance for clinical diagnosis and treatment of cat digestive tract diseases. The nucleic acid composition and the kit provided by the invention have the technical advantages of high detection sensitivity, good specificity and low detection limit, are short in detection time, and are beneficial to early diagnosis and treatment of diseased cats.

The invention discloses an integrated nucleic acid detection cartridge for feline coronavirus, comprising a box body, wherein a lysis chamber, a first cleaning chamber and a second cleaning chamber are sequentially arranged in the box body from top to bottom, wherein the lysis chamber is connected to the box cover, The second cleaning chamber is connected to the reaction chamber provided at the bottom of the outer side of the box body; the two connected sections of the cracking chamber, the first cleaning chamber, the second cleaning chamber and the reaction chamber are respectively provided with columns with the direction of the plunger holes perpendicular to the connection direction The plug, the plunger hole of the plunger is provided with a hydrophobic liquid sealing material; the reaction chamber is provided with an amplification reaction solution, and the amplification reaction solution includes a primer-probe mixture of the feline coronavirus ORF gene; the reaction chamber A fusible material layer is arranged in the middle, and a DNA polymerase solution is embedded in the soluble material layer. The present invention can rapidly and integrally carry out PCR amplification and detection of feline coronavirus under the condition of relatively low cost, and solve the problems of poor repeatability of the current technology, time-consuming detection method, complicated operation and the like.

The invention discloses use of conivaptan hydrochloride in the preparation of a drug for treating feline infectious peritonitis. Conivaptan hydrochloride has very low toxicity to feline normal cells and has an obvious inhibition effect to feline coronavirus and is capable of obviously reducing virulence of viruses in the cells; an indirect immunofluorescence detection result shows that conivaptanhydrochloride can effectively inhibit the proliferation of the feline coronavirus in the cells and has dose dependence. Therefore, conivaptan hydrochloride can be used for preparing a feline coronavirus inhibitor for treating feline infectious peritonitis and has the advantages of good treatment effect, high safety and the like.