Cat coronavirus integrated nucleic acid detection card box

A feline coronavirus and detection card technology, applied in the field of medical detection, can solve problems such as poor repeatability, time-consuming detection methods, and complicated operations

Active Publication Date: 2021-05-07
杭州遂曾生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can perform rapid and integrated PCR amplification and detection of feline coronavirus at a relatively low cost, and solve the problems of poor repeatability of the current technology, time-consuming detection methods, and complicated operations.

Method used

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  • Cat coronavirus integrated nucleic acid detection card box
  • Cat coronavirus integrated nucleic acid detection card box
  • Cat coronavirus integrated nucleic acid detection card box

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Embodiment Construction

[0032] The present invention will be further described below in conjunction with the accompanying drawings and embodiments, but not as a basis for limiting the present invention.

[0033] Example. A kind of cat coronavirus integrated nucleic acid detection cartridge, such as figure 1As shown: a box body 1 with a box cover 2 on the top, a lysis chamber 3 with a lysis solution, a first cleaning chamber 4 with a first cleaning solution, and a second cleaning solution. The second cleaning chamber 5, wherein the cracking chamber 3 is connected to the box cover 2, and the second cleaning chamber 5 is connected to the reaction chamber 6 provided at the outer bottom of the box body 1; the cracking chamber 3, the first cleaning chamber 4, the second cleaning chamber Plungers 7 whose plunger hole directions are perpendicular to the connection direction are respectively arranged in the pairwise connected intervals of the chamber 5 and the reaction chamber 6, and hydrophobic liquid seali...

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Abstract

The invention discloses a cat coronavirus integrated nucleic acid detection card box. The cat coronavirus integrated nucleic acid detection card box comprises a box body, wherein a cracking cavity, a first cleaning cavity and a second cleaning cavity are sequentially arranged in the box body from top to bottom, the cracking cavity communicates with a box cover, and the second cleaning cavity communicates with a reaction cavity arranged at the bottom of the outer side of the box body; plungers with plunger hole directions perpendicular to a connecting direction are respectively arranged in every two connecting intervals of the cracking cavity, the first cleaning cavity, the second cleaning cavity and the reaction cavity, and hydrophobic liquid sealing materials are arranged in plunger holes of the plungers; amplification reaction liquid is arranged in the reaction cavity and comprises primer probe mixed liquid of a cat coronavirus ORF gene; and a soluble material layer is arranged in the reaction cavity, and a DNA polymerase solution is embedded in the soluble material layer. The cat coronavirus integrated nucleic acid detection card box disclosed by the invention can quickly and integrally perform PCR amplification and detection on cat coronavirus under the condition of relatively low cost, and solves the problems of poor repeatability, time consumption of a detection method, complicated operation and the like in the prior art.

Description

technical field [0001] The invention relates to a cat coronavirus integrated nucleic acid detection cartridge, which belongs to the technical field of medical detection. Background technique [0002] Feline coronavirus infection is an infectious disease caused by feline coronavirus. FCoV is known to have two biotypes: feline infectious peritonitis virus (FIPV) and feline enteric coronavirus (FECV). Although both FIPV and FECV can cause viremia, only FIPV can replicate in macrophages and cause feline infectious peritonitis (Feline Infectious Peritonitis, FIP). FIP is a chronic progressive fatal infectious disease of feline animals caused by feline infectious peritonitis virus (FIPV) infection. It is characterized by peritonitis and accumulation of massive ascites. FIP is a disease with various clinical manifestations. [0003] At present, there are mainly the following methods for the detection of cat coronavirus: [0004] (1) Electron microscope detection: The virus (FCoV)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12M1/00C12Q1/70C12Q1/686
CPCC12Q1/701C12Q1/686C12Q2600/166C12Q2521/101C12Q2545/101C12Q2527/125C12Q2563/143C12Q2563/149Y02A50/30
Inventor 赵怀赵志敬华灿忠李青青
Owner 杭州遂曾生物技术有限公司
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