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Integrated nucleic acid detection cassette for African swine fever virus

An African swine fever virus, detection card technology, applied in biochemical equipment and methods, resistance to vector-borne diseases, and microbial determination/inspection, etc., can solve the problems of time-consuming, complex operation, and poor repeatability of detection methods

Active Publication Date: 2021-05-11
杭州遂曾生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can perform rapid and integrated PCR amplification and detection of African swine fever virus at a relatively low cost, and solve the problems of poor repeatability of the current technology, time-consuming detection methods, and complicated operations.

Method used

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  • Integrated nucleic acid detection cassette for African swine fever virus
  • Integrated nucleic acid detection cassette for African swine fever virus
  • Integrated nucleic acid detection cassette for African swine fever virus

Examples

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Embodiment Construction

[0033] The present invention will be further described below in conjunction with the accompanying drawings and embodiments, but not as a basis for limiting the present invention.

[0034] Example. A kind of African swine fever virus integrated nucleic acid detection cartridge, such as figure 1As shown: a box body 1 with a box cover 2 on the top, a lysis chamber 3 with a lysis solution, a first cleaning chamber 4 with a first cleaning solution, and a second cleaning solution. The second cleaning chamber 5, wherein the cracking chamber 3 is connected to the box cover 2, and the second cleaning chamber 5 is connected to the reaction chamber 6 provided at the outer bottom of the box body 1; the cracking chamber 3, the first cleaning chamber 4, the second cleaning chamber Plungers 7 whose plunger hole directions are perpendicular to the connection direction are respectively arranged in the pairwise connected intervals of the chamber 5 and the reaction chamber 6, and hydrophobic li...

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Abstract

The invention discloses an integrated nucleic acid detection cassette for African swine fever virus, which comprises a cassette body, a lysis cavity, a first cleaning cavity and a second cleaning cavity are sequentially arranged in the cassette body from top to bottom, the lysis cavity is communicated to a cassette cover, and the second cleaning cavity is communicated to a reaction cavity arranged at the bottom of the outer side of the cassette body; plungers with the plunger hole direction perpendicular to the connecting direction are arranged in the pairwise connecting intervals of the lysis cavity, the first cleaning cavity, the second cleaning cavity and the reaction cavity respectively, and hydrophobic liquid sealing materials are arranged in the plunger holes of the plungers; an amplification reaction solution is arranged in the reaction cavity; and a soluble material layer is arranged in the reaction cavity, and a primer probe mixed solution for VP72 gene of African swine fever virus is embedded in the soluble material layer. According to the invention, PCR amplification and detection can be rapidly and integrally carried out on the African swine fever virus under the condition of relatively low cost, and the problems of poor repeatability, time consumption of a detection method, complex operation and the like in the prior art are solved.

Description

technical field [0001] The invention relates to an African swine fever virus integrated nucleic acid detection cartridge, which belongs to the technical field of medical detection. Background technique [0002] African swine fever (African swine fever, ASF) is an acute, febrile, highly contagious zoonotic disease of pigs caused by African swine fever virus (ASFV), clinically characterized by high fever and reticuloendothelial bleeding Characterized by high mortality and high mortality, it affects pigs of all ages, and the case fatality rate of susceptible pigs is as high as 100%, which is seriously harmful to the pig industry, and is listed as a category A that must be reported by the World Organization for Animal Health (OIE) animal disease. [0003] ASFV is the only member of the African swine fever virus (Asfarviridae) genus (Asfivirus). It has a double-layer envelope structure and is a linear double-stranded DNA. There are 1 serotype and 22 genotypes. Its genome is abou...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/686C12N15/10C12N15/11
CPCC12Q1/701C12Q1/686C12N15/1013Y02A50/30
Inventor 赵怀赵志敬华灿忠卜柯丽
Owner 杭州遂曾生物技术有限公司
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