A cat coronavirus integrated nucleic acid detection cartridge

Abstract

The invention discloses an integrated nucleic acid detection cartridge for feline coronavirus, comprising a box body, wherein a lysis chamber, a first cleaning chamber and a second cleaning chamber are sequentially arranged in the box body from top to bottom, wherein the lysis chamber is connected to the box cover, The second cleaning chamber is connected to the reaction chamber provided at the bottom of the outer side of the box body; the two connected sections of the cracking chamber, the first cleaning chamber, the second cleaning chamber and the reaction chamber are respectively provided with columns with the direction of the plunger holes perpendicular to the connection direction The plug, the plunger hole of the plunger is provided with a hydrophobic liquid sealing material; the reaction chamber is provided with an amplification reaction solution, and the amplification reaction solution includes a primer-probe mixture of the feline coronavirus ORF gene; the reaction chamber A fusible material layer is arranged in the middle, and a DNA polymerase solution is embedded in the soluble material layer. The present invention can rapidly and integrally carry out PCR amplification and detection of feline coronavirus under the condition of relatively low cost, and solve the problems of poor repeatability of the current technology, time-consuming detection method, complicated operation and the like.

Description

technical field [0001] The invention relates to a cat coronavirus integrated nucleic acid detection cartridge, which belongs to the technical field of medical detection. Background technique [0002] Feline coronavirus infection is an infectious disease caused by Feline Coronavirus. There are two known biotypes of FCoV: Feline Infectious Peritonitis Virus (FIPV) and Feline Enteric Coronavirus (FECV). Although both FIPV and FECV can cause viremia, only FIPV can replicate in macrophages and cause Feline Infectious Peritonitis (FIP). FIP is a chronic progressive lethal infectious disease of felines caused by feline infectious peritonitis virus (FIPV) infection. It is mainly characterized by peritonitis and accumulation of a large amount of ascites. [0003] At present, the detection methods for feline coronavirus mainly include the following: [0004] (1) Electron microscope detection: The virus (FCoV) can be detected from feces by electron microscope observation. [0005] (...

AI Technical Summary

Problems solved by technology

It can perform rapid and integrated PCR amplification and detection of feline coronavirus at a relatively low cost, and solve the problems of poor repeatability of the current technology, time-consuming detection methods, and complicated operations.

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