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Triple detection card box for dengue, Zika and chikungunya viruses

A chikungunya virus and Zika virus technology, applied in the field of medical detection, can solve problems such as complicated operation, poor repeatability, and time-consuming detection methods, and achieve the effect of protecting safety, reducing requirements, and eliminating equipment pollution problems

Active Publication Date: 2021-05-07
杭州遂曾生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can quickly and integratedly perform PCR amplification and detection of dengue, Zika and chikungunya fever viruses at a relatively low cost, and solve the problems of poor repeatability of current technologies, time-consuming detection methods, and complicated operations.

Method used

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  • Triple detection card box for dengue, Zika and chikungunya viruses
  • Triple detection card box for dengue, Zika and chikungunya viruses
  • Triple detection card box for dengue, Zika and chikungunya viruses

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Embodiment Construction

[0033] The present invention will be further described below in conjunction with the accompanying drawings and embodiments, but not as a basis for limiting the present invention.

[0034] Example. A triplex test kit for Dengue, Zika, and Chikungunya viruses, such as figure 1 As shown: a box body 1 with a box cover 2 on the top, a lysis chamber 3 with a lysis solution, a first cleaning chamber 4 with a first cleaning solution, and a second cleaning solution. The second cleaning chamber 5, wherein the cracking chamber 3 is connected to the box cover 2, and the second cleaning chamber 5 is connected to the reaction chamber 6 provided at the outer bottom of the box body 1; the cracking chamber 3, the first cleaning chamber 4, the second cleaning chamber Plungers 7 whose plunger hole directions are perpendicular to the connection direction are respectively arranged in the pairwise connected intervals of the chamber 5 and the reaction chamber 6, and hydrophobic liquid sealing mater...

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Abstract

The invention discloses a triple detection card box for dengue, Zika and chikungunya viruses. The triple detection card box comprises a box body with a box cover at the top, wherein a cracking cavity, a first cleaning cavity and a second cleaning cavity are sequentially arranged in the box body from top to bottom; the cracking cavity communicates with the box cover; the second cleaning cavity communicates with a reaction cavity formed in the bottom of the outer side of the box body; plungers with plunger hole directions vertical to the connecting direction are respectively arranged in pairwise connecting regions of the cracking cavity, the first cleaning cavity, the second cleaning cavity and the reaction cavity; hydrophobic liquid sealing materials are arranged in plunger holes of the plungers; and amplification reaction liquid is arranged in the reaction cavity, and comprises RNA-directTM Realtime PCR Master Mix and primer probe mixed liquid of dengue viruses, Zika viruses and chikungunya viruses. The triple detection card box has the advantages that under the condition of relatively low cost, the dengue, Zika and chikungunya viruses can be fast and integrally subjected to PCR amplification and detection; and the problems of poor repeatability, time consumption of the detection method, operation complexity and the like in the prior art are solved.

Description

technical field [0001] The invention relates to a triple detection cartridge for dengue, Zika and chikungunya viruses, belonging to the technical field of medical detection. Background technique [0002] Dengue virus (DENV) and Zika virus (Zika virus, ZIKV) are both mosquito-borne viruses of the family Flaviviridae and the genus Flavivirus, which belong to single positive-sense RNA viruses. Chikungunya virus (Chikungunya virus, CHIKV) belongs to the Togaviridae Alphavirus genus. It is a single-stranded positive-sense RNA virus with an envelope and is also transmitted by mosquitoes. The clinical manifestations of Zika virus are mild fever, rash (mostly maculopapular), headache, arthralgia, and conjunctivitis. It can also cause microcephaly, Guillain-Barré syndrome, and other fetal central nervous system malformations. The clinical manifestations of dengue virus are fever, headache, muscle and joint pain all over the body, etc. Clinical manifestations of Chikungunya virus in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/686C12N15/11
CPCC12Q1/701C12Q1/686C12Q2561/113C12Q2563/107C12Q2537/143
Inventor 赵怀封帆华灿忠查丽虹
Owner 杭州遂曾生物技术有限公司
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