The invention relates to the technical field of polypeptide extraction, and discloses a method for extracting
crocodile nail
peptide with an anti-
radiation effect, which comprises the following steps:
degreasing and crushing
crocodile nails to obtain
fine powder, and performing
solid-phase kneading on the
fine powder and an enzymatic extraction auxiliary composition under an
anhydrous condition; adding water, homogenizing, and treating by a high-
voltage pulse electric field to obtain a flexible precursor; sequentially adding
keratinase and
alkaline protease to carry out constant pH
relay hydrolysis in a
nitrogen-filled micro-
positive pressure environment; cooling, adjusting the pH to an
isoelectric point, performing coagulation, separating supernate, performing
gradient elution through macroporous adsorption resin, and performing vacuum concentration to remove
alcohol; finally, the concentrated solution is screened through 3000 Da and 500 Da
ultrafiltration membrane bags in sequence, trapped fluid is collected,
freeze drying is conducted, and the product is obtained. According to the preparation method disclosed by the invention, the
dissolution rate and the purity of the
crocodile methyl
peptide with the anti-
radiation effect are effectively improved by combining
solid-phase kneading, a high-
voltage electric field with double-
enzyme hydrolysis and physical screening, and inactivation and loss of active ingredients are avoided.