RNA vaccine for feline coronavirus and construction method of RNA vaccine

A feline coronavirus and vaccine technology, applied in the field of RNA vaccines, achieves high nucleic acid encapsulation efficiency, high-efficiency expression, and shortens the vaccine development and production cycle

Active Publication Date: 2021-09-17
东莞博盛生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The infection rate of FECV is 80%-95%, while the fatality rate of FIP is as high as 95%. Currently, there is no effective vaccine on the market. Once a cat is infected with FIPV, it can almost only be delayed by treatment. Therefore, the market for FCoV vaccine is very potential, and Important for FCoV prevention

Method used

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  • RNA vaccine for feline coronavirus and construction method of RNA vaccine
  • RNA vaccine for feline coronavirus and construction method of RNA vaccine
  • RNA vaccine for feline coronavirus and construction method of RNA vaccine

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Experimental program
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Embodiment

[0039] A kind of RNA vaccine and its construction for feline coronavirus:

[0040] 1. Construction of recombinant bacteria:

[0041] Select 5'UTR, 3'UTR, S protein (S protein of UU4 strain in FCoV-I (GenBank: FJ938054.1)), stop codon, polyA nucleic acid sequence to artificially synthesize RNA vaccine DNA on pUC57 vector , seamlessly cloned into the EcoRV restriction site of pUC57, and transformed Escherichia coli DH5α to obtain FCoV-S-pUC57-DH5α;

[0042] The S protein described in this example is selected from the UU4 strain S protein (GenBank: FJ938054.1) in FCoV-I type, and the S protein can also be selected from FCoV-II type, or it can be the selected S protein An amino acid sequence or an amino acid sequence with 50-100% homology, the operation is the same as above, and will not be repeated here;

[0043] 2. Extraction of Plasmids

[0044] Shake the obtained recombinant bacteria, and extract the plasmid according to the conventional method, referring to the instruction...

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Abstract

The invention belongs to the technical field of RNA vaccines, and particularly relates to an RNA vaccine for feline coronavirus and a construction method of the RNA vaccine. The RNA vaccine is synthesized by adopting a sequence containing 5' UTR (Untranslated Region), S protein, a termination codon, 3' UTR and polyA nucleic acid; the S protein is selected from an FCoV-I type or an FCoV-II type; and the sequences of the 5' UTR and the 3' UTR are selected from feline hemoglobin or feline albumin of a feline source, and the homology of the selected sequences is 50%-100%. According to the RNA vaccine, cellular immunity and humoral immunity can be induced at the same time, cell-free production can be achieved, toxic chemicals do not need to be introduced in the production process, virus variation can be efficiently and rapidly treated, compared with a traditional vaccine, the RNA vaccine for the feline coronavirus can effectively shorten the vaccine research and development and production cycle, and an effective technical means is provided for preventing and controlling the outbreak of the FCoV.

Description

technical field [0001] The invention belongs to the technical field of RNA vaccines, in particular to an RNA vaccine for feline coronavirus and a construction method thereof. Background technique [0002] Feline coronavirus (FCoV) is the causative agent of feline infectious peritonitis (FIP), a fatal disease in domestic cats and wild cats. It is transmitted through the mouth and nose and mainly infects young and old cats. Popularity. [0003] FCoV virus has four main structural proteins, namely spike protein (Spike protein, S), membrane protein (Membrane protein, M), small envelope protein (Small envelope protein, E) and nucleocapsid protein (Nucleocapsid protein, N ). Among them, the S protein is a glycoprotein arranged on the envelope protrusion, with a molecular weight of about 180-200kDa, which is very important for inducing the host's antibody response and cellular immunity. Coronaviruses can bind to specific cell receptors through the S protein, which is a key facto...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/50A61K39/215A61P31/14
CPCC07K14/005A61K39/12A61P31/14C12N2770/20022A61K2039/53Y02A50/30
Inventor 刘昕赖强王弋吴培枫陈怡林郑飞吴先戈欧海航
Owner 东莞博盛生物科技有限公司
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