36 results about "Canine parainfluenza virus" patented technology

This invention relates to vaccines and methods for protecting dogs against disease caused by Bordetella bronchiseptica. This invention also relates to combination vaccines and methods for protecting dogs against disease or disorder caused by canine pathogens, for example, infectious tracheobronchitis caused by Bordetella bronchiseptica, canine distemper caused by canine distemper (CD) virus, infectious canine hepatitis (ICH) caused by canine adenovirus type 1 (CAV-1), respiratory disease caused by canine adenovirus type 2 (CAV-2), canine parainfluenza caused by canine parainfluenza (CPI) virus, enteritis caused by canine coronavirus (CCV) and canine parvovirus (CPV), and leptospirosis caused by Leptospira Bratislava, Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae or Leptospira pomona. The vaccines of the present invention include a Bordetella bronchiseptica p68 antigen.

The invention provides a detection kit and an application thereof and belongs to the field of biological detection. The kit comprises effective doses of anti-CDV (canine distemper virus) monoclonal antibodies 1 and 2, effective doses of anti-CPIV (canine parainfluenza virus) monoclonal antibodies 3 and 4 as well as a detection reagent for detecting an antigen-antibody reaction, wherein the monoclonal antibodies 1 and 2 as well as the monoclonal antibodies 3 and 4 can be subjected to the antigen-antibody reaction with CDV and CPIV respectively at the same time. The kit can effectively detect the two viruses and antigens of the two viruses, when enzyme reaction is adopted for detection, the sensitivity of the enzyme reaction is equivalent to that of PCR (polymerase chain reaction), and when colloidal gold is adopted for detection, the sensitivity of the colloidal gold is better that that of commercially available similar products. The invention further provides the application of the kit in the aspect of non-diagnostic purpose.

The invention provides a preparation method of canine interferon alpha 2 recombinant protein. The preparation method comprises following steps: canine interferon alpha 2 gene is subjected to codon optimization, and cloning into pET-21a or pET-32a for construction of recombinant expression plasmids is carried out, transformation into competent escherichia coli cells BL21 is carried out, preparationof recombinant bacteria is carried out, and then fermentation, inducible expression, and purifying are carried out so as to obtain the canine interferon alpha 2 recombinant protein. The canine interferon alpha 2 recombinant protein can be taken as a wide spectrum antiviral preparation, and possesses obvious preventing and controlling effect on infection of canine distemper virus, canine parvovirus disease, canine parainfluenza virus, and infectious canine hepatitis virus. The preparation method is capable of providing an effective technical mean for realization of recombinant canine interferon alpha 2 technological production and further broad spectrum prevention of canine virus.

The invention discloses a quadruple PCR detection method capable of identifying canine parvovirus, hundestaupe virus, canine parainfluenza virus and canine adenovirus type-2, belonging to the field of virus detection. With the adoption of the method, four viruses including canine parvovirus, hundestaupe virus, canine parainfluenza virus and canine adenovirus type-2 can be simultaneously detected, the operation is simple, the detection speed is high, and the throughput is high; the accuracy is high, the specificity is good, the repeatability is good, the analysis can be accurately and rapidly carried out at high throughput, and the method is suitable for being popularized and applied in clinical practice. A primer provided by the invention is good in specificity, can be combined with the four target viral nucleic acids needing to be detected, and can not be combined with other common viral nucleic acids, such as feline distemper, canine adenovirus type I and leptospira canicola.

The invention relates to a canine infectious tracheobronchitis bivalent live vaccine and a preparation method thereof. The preparation method comprises the following steps of: purifying canine pzrainfluenzavirus and bordetella bronchiseptica with high immunogenicity separated out by the inventor; and performing passage attenuation to obtain a low virulent strain CPIV-VY-c and a bacterial strain Bb-SD25d for serving as production seed cultures of viruses to prepare a safe and effective canine infectious tracheobronchitis bivalent live vaccine. Due to the adoption of the strain, canine infectious tracheobronchitis (kennel cough) caused by canine pzrainfluenzavirus and bordetella bronchiseptica can be prevented effectively specific to the conventional epidemic status of canine infectious tracheobronchitis.

This invention relates to vaccines and methods for protecting dogs against disease caused by Bordetella bronchiseptica. This invention also relates to combination vaccines and methods for protecting dogs against disease or disorder caused by canine pathogens, for example, infectious tracheobronchitis caused by Bordetella bronchiseptica, canine distemper caused by canine distemper (CD) virus, infectious canine hepatitis (ICH) caused by canine adenovirus type 1 (CAV-1), respiratory disease caused by canine adenovirus type 2 (CAV-2), canine parainfluenza caused by canine parainfluenza (CPI) virus, enteritis caused by canine coronavirus (CCV) and canine parvovirus (CPV), and leptospirosis caused by Leptospira Bratislava, Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae or Leptospira pomona. The vaccines of the present invention include a Bordetella bronchiseptica p68 antigen.

The invention discloses dual RT-PCR (reverse transcription-polymerase chain reaction) detection for a CDV (canine distemper virus) and a CPIV (canine parainfluenza virus) and a special primer for the detection. The invention provides the special primer for virus detection. The primer comprises a primer pair A and a primer pair B, wherein the primer pair A comprises a primer 1 and a primer 2; the primer pair B comprises a primer 3 and a primer 4; and nucleotide sequences of the primer 1, primer 2, primer 3 and the primer 4 are sequence 1, sequence 2, sequence 3 and sequence 4 respectively in a sequence table. Experiments prove that the special primer for dual RT-PCR detection for the CDV and the CPIV has good sensibility, can at least detect the RNA (ribonucleic acid) content to be about 1*10<-3>ng / mu L, the sensitivity is far higher than that of 1 ng / mu L. A dual RT-PCR detection method is simple, convenient, fast, economical and efficient, and has good application prospect.

The invention provides a canine interferon-alpha6 alpha7 recombinant protein and a preparation method and an application thereof. An amino acid sequence of the canine interferon-alpha6 alpha7 recombinant protein is shown as SEQ ID NO:6, the preparation method comprises the following steps: performing codon optimization on a canine interferon-alpha6 gene and a canine interferon-alpha7 gene, splicing the optimized gene sequence, cloning the gene to pPICZalphaA for construction of recombinant expression plasmids, converting pichia yeast, and preparing recombinant bacteria, and performing steps offermentation, inducible expression, purifying to obtain the canine interferon-alpha6 alpha7 recombinant protein. The canine interferon-alpha6alpha7 recombinant protein can be used as a wide spectrumantiviral preparation, has obvious prevention and treatment effects for canine distemper, canine parvovirus disease, canine parainfluenza virus infection, and canine infectious hepatitis virus infection, and provides an effective technical means for realizing recombinant canine interferon-alpha6 alpha7 series-connection recombinant protein production and further broad-spectrum prevention and canine viral epidemic disease treatment.

The invention provides a canine recombinant interferon-lambda 1, and a preparation method and an application thereof. The canine recombinant interferon-lambda 1 has a nucleotide sequence as shown in SEQ ID NO. 1. The invention also provides a preparation method for the canine recombinant interferon-lambda 1. According to the invention, a pichia pastoris strain expression system is utilized to express a recombinant yeast engineering strain containing a codon-optimized canine recombinant interferon-lambda 1 gene, so secretion-expressed canine recombinant interferon-lambda 1 with high activity isobtained. The canine recombinant interferon-lambda 1 prepared by using the method provided by the invention has high purity, can reach a purity of 0.86 mg / ml after purification, has a protein yield of 42 mg in 1 L of recombinant yeast engineering strains, has a specific antiviral activity of 2.85 x 10<7> IU / mg, can effectively prevent and treat infectious diseases like canine distemper, canine parvovirus, canine parainfluenza virus infection and canine infectious hepatitis, and has high safety and good application prospects.

The invention relates to a canine parainfluenza virus strain and application thereof. The canine parainfluenza virus HeN0718 strain has strong toxicity and good immunogenicity. A vaccine composition prepared from an inactive antigen of the canine parainfluenza virus HeN0718 strain can immunize an animal to allow the animal to rapidly produce an antibody. The vaccine composition can be effective in prevention and / or treatment of current canine parainfluenza related diseases. The vaccine composition is safe and can be combined with other antigens.

The invention discloses a multiplex PCR primer for detecting four viruses of a dog, a detection method and application. The primer comprises primers CCV-F and CCV-R for detecting a canine coronavirus,primers CPIV-F and CPIV-R for detecting a canine parainfluenza virus, and primers CAV-F and CAV-R for detecting a canine adenovirus type I and a canine adenovirus type II. The PCR detection method established by the primer can detect a single virus, can also simultaneously detect mixed infection of four viruses CCV, CPIV, CAV-1 and CAV-2, has the advantages of high detection sensitivity, good specificity and the like, can reduce the occurrence of false positive, can realize rapid, accurate, sensitive and early discovery of pathogen targets, provides technical support for establishing a standardized detection method for health monitoring of the dog, and can greatly save time and cost.

The invention provides a vaccine composition. The vaccine composition comprises a dog parainfluenza virus antigen with the immunity effective dose, a dog bordetella bronchiseptica antigen with the immunity effective dose, and an adjuvant, wherein the dog parainfluenza virus antigen is selected from an inactivated dog parainfluenza virus antigen or at least one of F protein, HN protein, M protein, NP protein, P protein and L protein, and the bordetella bronchiseptica antigen is selected from inactivated dog bordetella bronchiseptica antigen or p68 protein. Compared with the vaccines in the prior art, the vaccine composition provided by the invention has the better immune effect, and has no side effects, and thus the risk that a veterinarian worker or a contactor is infected is reduced. The invention further relates to a kit. The kit contains the inactivated dog parainfluenza virus antigen or the subunit antigen, the attenuated live dog bordetella bronchiseptica antigen or the attenuated live dog parainfluenza virus antigen, and the inactivated dog bordetella bronchiseptica antigen or p68 protein.

The invention discloses dual RT-PCR (reverse transcription-polymerase chain reaction) detection for a CDV (canine distemper virus) and a CPIV (canine parainfluenza virus) and a special primer for the detection. The invention provides the special primer for virus detection. The primer comprises a primer pair A and a primer pair B, wherein the primer pair A comprises a primer 1 and a primer 2; the primer pair B comprises a primer 3 and a primer 4; and nucleotide sequences of the primer 1, primer 2, primer 3 and the primer 4 are sequence 1, sequence 2, sequence 3 and sequence 4 respectively in a sequence table. Experiments prove that the special primer for dual RT-PCR detection for the CDV and the CPIV has good sensibility, can at least detect the RNA (ribonucleic acid) content to be about 1*10<-3>ng / mu L, the sensitivity is far higher than that of 1 ng / mu L. A dual RT-PCR detection method is simple, convenient, fast, economical and efficient, and has good application prospect.

The invention discloses a reaction solution capable of improving specificity of an inverse transcription nucleic acid chain type amplification reaction, primer pair, probe and kit for detecting canineparainfluenza. A method for improving the specificity of the inverse transcription nucleic acid chain type amplification reaction comprises the steps that in an inverse transcription reaction stage,recA protein and ATP are utilized for ensuring and improving the specificity of an inverse transcription product; compared with the prior art, the specificity of the inverse transcription product is significantly improved, and the non-specific amplification phenomenon does not occur on the inverse transcription product obtained by adopting the method in a later PCR reaction; the kit for detectingthe canine parainfluenza virus adopts primers and the probe which are modified by amino groups, the specificity is improved, the detection time is shortened, the diagnosis capacity is ensured to the greatest extent, and convenience is provided for clinical use.

The invention relates to the technical field of molecular biomedicine, in particular to a PCR (Polymerase Chain Reaction) detection kit for cat and / or dog pathogens, a detection method and application. The kit is used for detecting canine distemper virus, canine influenza A virus, canine parainfluenza virus, canine parvovirus, canine coronavirus, canine rotavirus, canine babesia, canine ascaris, canine Ehrlichia, canine brucella, rabies virus, borrelia burgdorferi, reference gene ACTB, feline herpes virus, feline calicivirus and feline parvovirus. The kit comprises primers and probes of feline coronavirus, feline immunodeficiency virus, feline leukemia virus, feline mycoplasma, feline mycoplasma, feline chlamydia, giardia, toxoplasma, bartonella and reference gene GAPDH, collected DNA and RNA are added into the kit, a real-time fluorescence PCR instrument is adopted for PCR reaction, FAM, HEX, ROX and CY5 fluorescence signals are collected in each cycle, analysis of related pathogens is carried out, and the kit can be used for detecting the feline and the canine. Compared with a traditional detection method, the method has the advantages of higher specificity and higher sensitivity.

Provided herein are compositions comprising a canine influenza virus and a canine respiratory coronavirus. They can further comprise Bordetella bronchiseptica, pertactin, canine parainfluenza virus, and canine adenovirus serotype 2. The compositions are effective for treating or preventing canine respiratory diseases, including canine infectious respiratory disease complex.

The invention provides a multi-PCR (Polymerase Chain Reaction) detecting primer group for a canine distemper virus, a canine adenovirus type 2 and a canine parainfluenza virus. The multi-PCR detecting primer group comprises a primer pair A, a primer pair B and a primer pair C, wherein the primer pair A comprises a primer 1 and a primer 2; the primer pair B comprises a primer 3 and a primer 4; the primer pair C comprises a primer 5 and a primer 6; the nucleotide sequences of the primer 1, the primer 2, the primer 3, the primer 4, the primer 5 and the primer 6 are respectively a sequence 1, a sequence 2, a sequence 3, a sequence 4, a sequence 5 and a sequence 6 in sequence tables. The multi-PCR detecting primer group has the advantages of simplicity in operation, high sensitivity, strong specificity, good repeatability, low cost and time consumption and the like.

This invention relates to vaccines and methods for protecting dogs against disease caused by Bordetella bronchiseptica. This invention relates to vaccines and methods for protecting dogs against disease caused by Leptospira bratislava. This invention also relates to combination vaccines and methods for protecting dogs against disease or disorder caused by canine pathogens, for example, infectious tracheobronchitis caused by Bordetella bronchiseptica, canine distemper caused by canine distemper (CD) virus, infectious canine hepatitis (ICH) caused by canine adenovirus type 1 (CAV-1), respiratory disease caused by canine adenovirus type 2 (CAV-2), canine parainfluenza caused by canine parainfluenza (CPI) virus, enteritis caused by canine coronavirus (CCV) and canine parvovirus (CPV), and leptospirosis caused by Leptospira bratislava, Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae or Leptospira pomona.

The invention discloses a primer, a probe and a kit for detecting canine distemper virus of a giant panda. Based on the design of the primer and the probe, the canine distemper virus of the giant panda (giant panda / SX / 2014 strain) (Genbank:KP793921) is used as a template, the detection specificity is improved, and cross reaction between the canine distemper virus of the giant panda and other viruses such as canine parvovirus (CPV), canine coronavirus (CCV), canine parainfluenza virus (CPIV) and canine rotavirus (CRV) is avoided; when the primer and the probe are applied to detection of the canine distemper virus of the giant panda, the sensitivity is high, a detection limit is 5x101copy / microliters, and a detection result is reliable; and compared with an existing RT-PCR method, the primerand the probe are very high in conformity, and can realize portable quick diagnosis on site.

The invention discloses a kit for detecting a canine parainfluenza virus. The kit comprises a light shielding detection box, ALL-READY PCR BEADS full premix freeze-dried powder, a positive control group, a negative control group and a photodetector, the light shielding detection box and the photodetector are arranged in a fitting manner, a photoelectric detection module is arranged in the photoelectric detector, and the ALL-READY PCR BEADS full premix freeze-dried powder comprises transcriptase, a nucleic acid amplifier enzyme, a reaction buffer solution, a specific primer and a probe which are needed by the fluorescent quantitative PCR detection of the canine parainfluenza virus RNA; and the negative control group adopts canine parainfluenza virus RNA-free enzyme water, the positive control group is in vitro transcribed parainfluenza virus RNA, and the photoelectric detection module includes a photodiode. The design is optimized, the light shielding detection box is combined, and the photoelectric detection module is optimized, so the detection error is reduced, and the detection specificity is improved.

The invention discloses the application of canine parainfluenza virus nucleocapsid protein in preparing an indirect ELISA kit for detecting canine parainfluenza virus antibody, and the invention also discloses an indirect ELISA kit for detecting canine parainfluenza virus antibody. The kit can be used for rapid detection of canine parainfluenza virus antibody in clinical canine serum samples and antibody monitoring of immunized dogs, and has the characteristics of high detection specificity, sensitivity and reproducibility.

The invention relates to a primer, an amplification reaction solution, a kit and a detection method for LAMP (loop-mediated isothermal amplification) detection of canine parainfluenza virus, the kit and the detection method can accurately distinguish samples containing canine parainfluenza virus and samples not containing canine parainfluenza virus, namely after LAMP specific detection, the specificity of the designed primer is good. According to the kit and the detection method, the canine parainfluenza virus can be quickly and accurately detected, results are observed through visual inspection or instruments, data processing is simple, cost is low, and the kit and the detection method have important significance on clinical detection, are particularly suitable for livestock farms, are economical in detection and can be widely popularized.

Problem to be Solved: The present invention provides a novel mucosal adjuvant.Solution: The present invention provides a mucosal adjuvant composition containing at least a composition comprising molecules having a molecular weight in a range of 100 to 300 kDa obtained from cells or culture fluid of Bordetella bronchiseptica. Administration of the mucosal adjuvant composition to a non-human animal at a surface of the mucous membrane can enhance immunity at the surface of mucous membrane. Therefore preventive effects against trans-mucosal infection can be increased by administering an inactivated vaccine against trans-mucosal infection and the mucosal adjuvant composition to a non-human animal at a surface of mucous membrane. The present invention is effective for preventing trans-mucosal infections, including one or more infections of e.g., canine parainfluenza, canine adenovirus, canine coronavirus, canine parvovirus, canine distemper virus, canine herpesvirus, reovirus and pneumovirus.

The invention relates to a canine infectious tracheobronchitis bivalent live vaccine and a preparation method thereof. The preparation method comprises the following steps of: purifying canine pzrainfluenzavirus and bordetella bronchiseptica with high immunogenicity separated out by the inventor; and performing passage attenuation to obtain a low virulent strain CPIV-VY-c and a bacterial strain Bb-SD25d for serving as production seed cultures of viruses to prepare a safe and effective canine infectious tracheobronchitis bivalent live vaccine. Due to the adoption of the strain, canine infectious tracheobronchitis (kennel cough) caused by canine pzrainfluenzavirus and bordetella bronchiseptica can be prevented effectively specific to the conventional epidemic status of canine infectious tracheobronchitis.

The invention relates to the field of veterinary drugs, in particular to a canine parainfluenza virus attenuated strain, application thereof and a vaccine. The invention provides the canine parainfluenza virus attenuated strain with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.19993. The canine parainfluenza virus attenuated strain disclosed by the invention is strong in pertinence, can be used for dogs of various varieties and various ages and also can be used for pregnant dogs, and the generated antibody is high and the immune period is long.

The invention discloses a quadruple PCR detection method capable of identifying canine parvovirus, hundestaupe virus, canine parainfluenza virus and canine adenovirus type-2, belonging to the field of virus detection. With the adoption of the method, four viruses including canine parvovirus, hundestaupe virus, canine parainfluenza virus and canine adenovirus type-2 can be simultaneously detected, the operation is simple, the detection speed is high, and the throughput is high; the accuracy is high, the specificity is good, the repeatability is good, the analysis can be accurately and rapidly carried out at high throughput, and the method is suitable for being popularized and applied in clinical practice. A primer provided by the invention is good in specificity, can be combined with the four target viral nucleic acids needing to be detected, and can not be combined with other common viral nucleic acids, such as feline distemper, canine adenovirus type I and leptospira canicola.

The invention relates to murine monoclonal antibodies 2B7 and 4H1 of canine parainfluenza virus, and a variable region sequence of the monoclonal antibody can be specifically and conservatively bond with the canine parainfluenza virus. A colloidal gold detection kit prepared by using the murine monoclonal antibodies as an antibody pair can detect a variety of epidemic strains and standard strainsof the canine parainfluenza virus with high sensitivity, has a high coincidence rate with the RT-PCR detection method, and can meet the urgent clinical needs at present.