36 results about "Canine parainfluenza virus" patented technology

The invention discloses a quadruple PCR detection method capable of identifying canine parvovirus, hundestaupe virus, canine parainfluenza virus and canine adenovirus type-2, belonging to the field of virus detection. With the adoption of the method, four viruses including canine parvovirus, hundestaupe virus, canine parainfluenza virus and canine adenovirus type-2 can be simultaneously detected, the operation is simple, the detection speed is high, and the throughput is high; the accuracy is high, the specificity is good, the repeatability is good, the analysis can be accurately and rapidly carried out at high throughput, and the method is suitable for being popularized and applied in clinical practice. A primer provided by the invention is good in specificity, can be combined with the four target viral nucleic acids needing to be detected, and can not be combined with other common viral nucleic acids, such as feline distemper, canine adenovirus type I and leptospira canicola.

The invention relates to a canine infectious tracheobronchitis bivalent live vaccine and a preparation method thereof. The preparation method comprises the following steps of: purifying canine pzrainfluenzavirus and bordetella bronchiseptica with high immunogenicity separated out by the inventor; and performing passage attenuation to obtain a low virulent strain CPIV-VY-c and a bacterial strain Bb-SD25d for serving as production seed cultures of viruses to prepare a safe and effective canine infectious tracheobronchitis bivalent live vaccine. Due to the adoption of the strain, canine infectious tracheobronchitis (kennel cough) caused by canine pzrainfluenzavirus and bordetella bronchiseptica can be prevented effectively specific to the conventional epidemic status of canine infectious tracheobronchitis.

This invention relates to vaccines and methods for protecting dogs against disease caused by Bordetella bronchiseptica. This invention also relates to combination vaccines and methods for protecting dogs against disease or disorder caused by canine pathogens, for example, infectious tracheobronchitis caused by Bordetella bronchiseptica, canine distemper caused by canine distemper (CD) virus, infectious canine hepatitis (ICH) caused by canine adenovirus type 1 (CAV-1), respiratory disease caused by canine adenovirus type 2 (CAV-2), canine parainfluenza caused by canine parainfluenza (CPI) virus, enteritis caused by canine coronavirus (CCV) and canine parvovirus (CPV), and leptospirosis caused by Leptospira Bratislava, Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae or Leptospira pomona. The vaccines of the present invention include a Bordetella bronchiseptica p68 antigen.

The invention discloses dual RT-PCR (reverse transcription-polymerase chain reaction) detection for a CDV (canine distemper virus) and a CPIV (canine parainfluenza virus) and a special primer for the detection. The invention provides the special primer for virus detection. The primer comprises a primer pair A and a primer pair B, wherein the primer pair A comprises a primer 1 and a primer 2; the primer pair B comprises a primer 3 and a primer 4; and nucleotide sequences of the primer 1, primer 2, primer 3 and the primer 4 are sequence 1, sequence 2, sequence 3 and sequence 4 respectively in a sequence table. Experiments prove that the special primer for dual RT-PCR detection for the CDV and the CPIV has good sensibility, can at least detect the RNA (ribonucleic acid) content to be about 1*10<-3>ng/mu L, the sensitivity is far higher than that of 1 ng/ mu L. A dual RT-PCR detection method is simple, convenient, fast, economical and efficient, and has good application prospect.

The invention provides a canine interferon-alpha6 alpha7 recombinant protein and a preparation method and an application thereof. An amino acid sequence of the canine interferon-alpha6 alpha7 recombinant protein is shown as SEQ ID NO:6, the preparation method comprises the following steps: performing codon optimization on a canine interferon-alpha6 gene and a canine interferon-alpha7 gene, splicing the optimized gene sequence, cloning the gene to pPICZalphaA for construction of recombinant expression plasmids, converting pichia yeast, and preparing recombinant bacteria, and performing steps offermentation, inducible expression, purifying to obtain the canine interferon-alpha6 alpha7 recombinant protein. The canine interferon-alpha6alpha7 recombinant protein can be used as a wide spectrumantiviral preparation, has obvious prevention and treatment effects for canine distemper, canine parvovirus disease, canine parainfluenza virus infection, and canine infectious hepatitis virus infection, and provides an effective technical means for realizing recombinant canine interferon-alpha6 alpha7 series-connection recombinant protein production and further broad-spectrum prevention and canine viral epidemic disease treatment.

The invention provides a canine recombinant interferon-lambda 1, and a preparation method and an application thereof. The canine recombinant interferon-lambda 1 has a nucleotide sequence as shown in SEQ ID NO. 1. The invention also provides a preparation method for the canine recombinant interferon-lambda 1. According to the invention, a pichia pastoris strain expression system is utilized to express a recombinant yeast engineering strain containing a codon-optimized canine recombinant interferon-lambda 1 gene, so secretion-expressed canine recombinant interferon-lambda 1 with high activity isobtained. The canine recombinant interferon-lambda 1 prepared by using the method provided by the invention has high purity, can reach a purity of 0.86 mg/ml after purification, has a protein yield of 42 mg in 1 L of recombinant yeast engineering strains, has a specific antiviral activity of 2.85 x 10<7> IU/mg, can effectively prevent and treat infectious diseases like canine distemper, canine parvovirus, canine parainfluenza virus infection and canine infectious hepatitis, and has high safety and good application prospects.

The invention relates to a canine parainfluenza virus strain and application thereof. The canine parainfluenza virus HeN0718 strain has strong toxicity and good immunogenicity. A vaccine composition prepared from an inactive antigen of the canine parainfluenza virus HeN0718 strain can immunize an animal to allow the animal to rapidly produce an antibody. The vaccine composition can be effective in prevention and/or treatment of current canine parainfluenza related diseases. The vaccine composition is safe and can be combined with other antigens.

The invention provides a vaccine composition. The vaccine composition comprises a dog parainfluenza virus antigen with the immunity effective dose, a dog bordetella bronchiseptica antigen with the immunity effective dose, and an adjuvant, wherein the dog parainfluenza virus antigen is selected from an inactivated dog parainfluenza virus antigen or at least one of F protein, HN protein, M protein, NP protein, P protein and L protein, and the bordetella bronchiseptica antigen is selected from inactivated dog bordetella bronchiseptica antigen or p68 protein. Compared with the vaccines in the prior art, the vaccine composition provided by the invention has the better immune effect, and has no side effects, and thus the risk that a veterinarian worker or a contactor is infected is reduced. The invention further relates to a kit. The kit contains the inactivated dog parainfluenza virus antigen or the subunit antigen, the attenuated live dog bordetella bronchiseptica antigen or the attenuated live dog parainfluenza virus antigen, and the inactivated dog bordetella bronchiseptica antigen or p68 protein.

The invention discloses a reaction solution capable of improving specificity of an inverse transcription nucleic acid chain type amplification reaction, primer pair, probe and kit for detecting canineparainfluenza. A method for improving the specificity of the inverse transcription nucleic acid chain type amplification reaction comprises the steps that in an inverse transcription reaction stage,recA protein and ATP are utilized for ensuring and improving the specificity of an inverse transcription product; compared with the prior art, the specificity of the inverse transcription product is significantly improved, and the non-specific amplification phenomenon does not occur on the inverse transcription product obtained by adopting the method in a later PCR reaction; the kit for detectingthe canine parainfluenza virus adopts primers and the probe which are modified by amino groups, the specificity is improved, the detection time is shortened, the diagnosis capacity is ensured to the greatest extent, and convenience is provided for clinical use.

Provided herein are compositions comprising a canine influenza virus and a canine respiratory coronavirus. They can further comprise Bordetella bronchiseptica, pertactin, canine parainfluenza virus, and canine adenovirus serotype 2. The compositions are effective for treating or preventing canine respiratory diseases, including canine infectious respiratory disease complex.

The invention provides a multi-PCR (Polymerase Chain Reaction) detecting primer group for a canine distemper virus, a canine adenovirus type 2 and a canine parainfluenza virus. The multi-PCR detecting primer group comprises a primer pair A, a primer pair B and a primer pair C, wherein the primer pair A comprises a primer 1 and a primer 2; the primer pair B comprises a primer 3 and a primer 4; the primer pair C comprises a primer 5 and a primer 6; the nucleotide sequences of the primer 1, the primer 2, the primer 3, the primer 4, the primer 5 and the primer 6 are respectively a sequence 1, a sequence 2, a sequence 3, a sequence 4, a sequence 5 and a sequence 6 in sequence tables. The multi-PCR detecting primer group has the advantages of simplicity in operation, high sensitivity, strong specificity, good repeatability, low cost and time consumption and the like.

The invention relates to a primer, an amplification reaction solution, a kit and a detection method for LAMP (loop-mediated isothermal amplification) detection of canine parainfluenza virus, the kit and the detection method can accurately distinguish samples containing canine parainfluenza virus and samples not containing canine parainfluenza virus, namely after LAMP specific detection, the specificity of the designed primer is good. According to the kit and the detection method, the canine parainfluenza virus can be quickly and accurately detected, results are observed through visual inspection or instruments, data processing is simple, cost is low, and the kit and the detection method have important significance on clinical detection, are particularly suitable for livestock farms, are economical in detection and can be widely popularized.

Problem to be Solved: The present invention provides a novel mucosal adjuvant.

Solution: The present invention provides a mucosal adjuvant composition containing at least a composition comprising molecules having a molecular weight in a range of 100 to 300 kDa obtained from cells or culture fluid of Bordetella bronchiseptica. Administration of the mucosal adjuvant composition to a non-human animal at a surface of the mucous membrane can enhance immunity at the surface of mucous membrane. Therefore preventive effects against trans-mucosal infection can be increased by administering an inactivated vaccine against trans-mucosal infection and the mucosal adjuvant composition to a non-human animal at a surface of mucous membrane. The present invention is effective for preventing trans-mucosal infections, including one or more infections of e.g., canine parainfluenza, canine adenovirus, canine coronavirus, canine parvovirus, canine distemper virus, canine herpesvirus, reovirus and pneumovirus.