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Canine infectious tracheobronchitis bivalent live vaccine and preparation method thereof

A bronchitis, infectious technology, applied in the field of canine infectious tracheobronchitis dual live vaccine and its preparation

Active Publication Date: 2013-02-13
ZHEJIANG YEBIO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been a lot of researches on the prevention and treatment of kennel cough in foreign countries, and there are many effective preventive products, but there is no vaccine against kennel cough in China at present, so this invention fills the blank of domestic kennel cough vaccine

Method used

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  • Canine infectious tracheobronchitis bivalent live vaccine and preparation method thereof
  • Canine infectious tracheobronchitis bivalent live vaccine and preparation method thereof
  • Canine infectious tracheobronchitis bivalent live vaccine and preparation method thereof

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Embodiment Construction

[0008] The present invention is mainly implemented through the following technical solutions:

[0009] 1. The source and characteristics of bacteria and viruses used in production

[0010] 1. Canine parainfluenza virus

[0011] (1) Isolation of canine parainfluenza virus

[0012] 1) The virus was isolated by the inventors from meat dog farms where kennel cough occurred in a large area. The collected nasal swabs were sterilized by filtration and inoculated on Vero cells (purchased from Cambivac, Co. Ltd) grown to the end of logarithmic phase. After static culture at 37° C. for 7 days and freezing and thawing twice, a suspension containing virus particles was obtained.

[0013] 2) Aspirate the above virus suspension, extract DNA and RNA templates, and identify by RT-PCR that the virus suspension contains canine parainfluenza virus.

[0014] According to the PCR identification of parainfluenza virus provided by Wang Fengxue et al. Primers and methods for amplification.

[0...

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Abstract

The invention relates to a canine infectious tracheobronchitis bivalent live vaccine and a preparation method thereof. The preparation method comprises the following steps of: purifying canine pzrainfluenzavirus and bordetella bronchiseptica with high immunogenicity separated out by the inventor; and performing passage attenuation to obtain a low virulent strain CPIV-VY-c and a bacterial strain Bb-SD25d for serving as production seed cultures of viruses to prepare a safe and effective canine infectious tracheobronchitis bivalent live vaccine. Due to the adoption of the strain, canine infectious tracheobronchitis (kennel cough) caused by canine pzrainfluenzavirus and bordetella bronchiseptica can be prevented effectively specific to the conventional epidemic status of canine infectious tracheobronchitis.

Description

technical field [0001] The invention relates to a dual live vaccine for canine infectious tracheobronchitis and a preparation method thereof, belonging to the field of veterinary biological products. Background technique [0002] Canine infectious tracheobronchitis (or kennel cough) is one of the most prevalent upper respiratory diseases in dogs. It is characterized by high infectivity and wide range of influence. The disease is now found in all parts of the world. Scholars generally believe that the main pathogens causing this disease are canine parainfluenza virus and Bordetella bronchiseptica (Ettinger, Stephen J.; Feldman, Edward C. (1995). Textbook of Veterinary Internal Medicine (4 th Edition). W.B. Saunders Company). Because the pathogen of this disease spreads very quickly among dogs, it is named "kennel cough". [0003] Canine parainfluenza virus (CPIV) belongs to the subfamily Paramyxoviridae and the genus Paramyxovirus. It has three specific antigens: surface...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/295A61K39/155A61K39/02A61P31/04A61P31/14
Inventor 嵇康张鹏超孟超沈元
Owner ZHEJIANG YEBIO BIOTECH
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