103results about How to "Enhanced antibody" patented technology

The present disclosure relates to a symmetric bispecific antibody of the class IgG4 comprising two heavy chains which each comprise a variable domain, CH1 domain and a hinge region, wherein in each heavy chain: the cysteine in the CH1 domain which forms an inter-chain disulphide bond with a cysteine in a light chain is substituted with another amino acid; and optionally one or more of the amino acids positioned in the upper hinge region is substituted with cysteine, wherein the constant region sequence of each heavy chain is similar or identical and the variable region in each heavy chain is different, formulations comprising the same, the use of each of the above in treatment and processes for preparing said antibodies and formulations.

The invention relates to a method for preparing five-classification leucocyte simulacrum particle by erythrocyte, comprising the following steps: selecting proper erythrocyte, processing the erythrocyte by multifunctional reagent to achieve the integrity of erythrocyte membrane and synchronous regulation of volume, shape and inclusion, intensifying and fixing the erythrocyte, and preserving afterwashing. The invention also relates to a leucocyte simulacrum particle prepared by the method, reagent system used for preparing the leucocyte simulacrum particle, and quality control substance and calibration substance used for hematology analyzer and containing the simulacrum particle.

The invention relates to a preparation for preventing and treating tapping panel dryness of rubber trees, which comprises the following components. The invention further relates to a preparation method and the application of the preparation. The preparation provided by the invention is a major breakthrough at home and abroad in the field of resurrection of tapping panel dryness of rubber trees currently. The preparation aims to blend nutrition of rubber trees, so that after the rubber trees absorb nutrition, antibodies are strengthened and dead skin is gradually recovered, and thus, rubber is normally discharged and yield of the rubber trees in China is improved.

The invention discloses a lateral reciprocating immunochromatography method for detecting biological samples and a diagnosis test strip and a diagnosis device based on the method. The diagnosis device comprises a card bottom, a card cover and a diagnosis test strip, wherein the diagnosis test strip comprises a PVC (Polyvinyl Chloride) plate, and a sample layer, a mark layer, a coated film, a water absorbing layer, a buffer solution sample layer and a water-proof isolating film which are stuck to the PVC plate; the surface of the water absorbing layer is covered with the water-proof isolating film and the sample layer in sequence; the water-proof isolating film is easy to extract; the buffer solution sample layer, the mark layer, the coated film and the water absorbing layer are stuck in sequence along a horizontal extraction direction of the water-proof isolating film; the water absorbing layer and the coated film are not overlapped with each other; and the card cover is respectively provided with a liquid dripping window, an observation window and a sample window at positions corresponding to the buffer solution sample layer, the coated film and the sample layer respectively. The diagnosis device disclosed by the invention is applied to reciprocating immunochromatography, and an antigen or an antibody in a sample undergoes a secondary reaction with antibodies or antigens of T and C lines on the coated film, so that the antigen and antibody reaction time is increased, and the sensitivity is increased by 50-100 times than the conventional unidirectional chromatography test strip.

The invention aims to provide a Muscovy duck parvovirus. The Muscovy duck parvovirus is a YBMDV strain, and is preserved in the China General Microbiological Culture Collection Center of the China Committee Of Culture Collection For Microorganisms in the Institute of Microbiology Chinese Academy of Science at the address of No. 3, No. 1 yard, Beichen west road, Chaoyang District, Beijing, on November 11th, 2013; the preservation number is CGMCC No. 8504. The Muscovy duck parvovirus screened by the invention has the characteristics of low toxicity and high immunogenicity; dead embryo and embryo liquid can be obtained by vaccinating the Muscovy duck parvovirus to a Muscovy duck embryo; virus liquid can be collected after grinding and freeze thawing; a vaccine is prepared by ultrafiltration concentration, formalin inactivation, the addition of an adjuvant, and mixing and emulsification; the Muscovy duck parvovirus can improve antibody of the Muscovy duck, ensure parent source antibody level of an offspring, and prevent green duck parvovirus infection caused by Muscovy duck parvovirus; the vaccine has the advantages of high efficiency and good safety.

The invention discloses a traditional Chinese medicine for silicosis treating. The traditional Chinese medicine comprises, by weight, 20-30 parts of lilies, 20-30 parts of tendril-leaved fritillary bulbs, 10-20 parts of radix paeoniae alba, 10-20 parts of sculellaria barbata, 10-20 parts of hedyotis diffusa, 10-15 parts of scutellaria baicalensis, 10-20 parts of gardenia, 10-20 parts of fructus forsythiae, 20-30 parts of cordate houttuynia, 10-20 parts of safflower carthamus, 5-10 parts of peach seeds, 10-20 parts of angelica sinensis, 20-30 parts of folium eriobotryae, 10-20 parts of almond, 10-15 parts of flos farfarae, 10-15 parts of radix stemonae, 10-20 parts of aster, 15-25 parts of platycodon grandiflorum, 10-20 parts of radix ophiopogonis, 20-30 parts of the roots of kudzu vine, 30-40 parts of astragalus membranaceus, 10-20 parts of mair conditioning unita, 10-20 parts of codonopsis pilosula, 5-10 parts of sappanwood, 10-15 parts of poria cocos, 10-15 parts of ligusticum wallichii, 10-15 parts of panax notoginseng and 5-10 parts of liquorice. The traditional Chinese medicine for silicosis treating can nourish lungs and kidneys and achieve internal and external therapy.

The liver protecting and kidney invigorating bean is one kind of health food prepared with black bean and 12 kinds of Chinese medicinal materials including rehmannia root, white atractylodes rhizome, Yunling, etc and salt in certain weight proportion. The preparation process includes decocting the Chinese medicinal materials, filtering, adding black bean into the filtrate, boiling, stoving black bean, and packing. The liver protecting and kidney invigorating bean has the health functions of protecting liver, invigorate spleen and stomach, promoting urination, tranquilizing, balancing yin-yang, etc.

The invention relates to a serum-5 type haemophilus lus paradis vaccine strain. The classified name of the vaccine strain is haemophilus lus paradis, the strain name is JSYZ10 and the vaccine strain is preserved in the China Center for Type Culture Collection on June 15, 2014 with the preservation number of CCTCC NO:M 2014260. The invention further relates to an application of the serum-5 type haemophilus lus paradis vaccine strain in preparation of a haemophilus lus paradis inactivated vaccine. The serum-5 type haemophilus lus paradis JSYZ10 strain is stable in biology, has a strong pathogenicity to a piglet, and has a good immunogenicity when being inactivated and vaccinated on the piglet. A univalent vaccine prepared from the vaccine strain serving as a vaccine candidate strain has good safety, can produce a relatively high antibody on the piglet, has long duration and good immune potency, and can be used for resisting attack of homotype wild strains. After a pig group is immunized, the morbidity and death rate are remarkably reduced, the economic loss of a piggery is reduced, and the immunizing effect of the vaccine strain is the same as or superior to that of existing commercial vaccines on the market.

Methods and compositions for enhancing an immune response to a selected antigen are described. The methods are useful for the treatment and prevention of microbial infections, such as infections caused by bacteria, viruses, fungi and parasites. The methods and compositions include host defense peptides, polyphosphazenes and immunostimulatory sequences to enhance the immune response to a coadministered antigen.

The invention provides a blood serum 5 type haemophilus parasuis strain JX1002. The blood serum 5 type haemophilus parasuis strain JX1002 is collected in China Center for Type Culture Collection (CCTCC), and the collection number is CCTCC M 2014127. The strain provided by the invention is stable in biological performance, has very strong pathogenicity to piglets, and has very good immunogenicity when the strain is inoculated to the piglets after inactivation. A monovalent vaccine prepared by using the strain as a vaccine candidate strain is good in safety, can be used for generating relatively high antibodies to the piglets, is long in duration, can be used for resisting the attack of wild strains with the same type, and has very good immune efficiency. An inactivated vaccine can be used for effectively reducing the occurrence of diseases and reducing the economic loss of a pig farm in clinical use, and the immune effect of the inactivated vaccine can reach or exceed that of existing commercial vaccines in markets.

Provided is a novel use of formaldehyde in preparing pharmaceuticals for prevention and cure of animal diseases caused by pathogenic microorganisms through infection of digestive canal by means of digestive tract administration, wherein formaldehyde can deactivate pathogenic microorganisms and their toxins, make it lose pathogenicity while keeping the antigenicity. The invention realizes the advantages of broad spectrum, quick and sure effects, small dosage, less administration, lower price and removal of drug resistance and medicinal residual produced by the application of antibiotics.

The invention relates to a floor with health function, which first forms a base layer, coats a layer of disinfection fungus-proof nanometer mineral or far-infrared coat on the base layer, dried and solidifies into one surface with fungus-proof function or far-infrared light function. The fungus-proof disinfection nanometer zinc oxide can disinfect via chemical function, while the active zinc ion can absorb the sulfhydryl group on the apoenzyme of thallus, and combine them quickly, to inactivate the enzyme of sulfhydryl group, to kill the bacterial, and the zinc ion can get off the dead bacterial, as long effect antibacterial agent. The activity of nanometer zinc oxide can be excited by the sunlight or daylight lamp, while the zinc oxide is nanometer level.

The invention belongs to the technical field of cosmetics, and particularly relates to polypeptide repair liquid and a preparation method thereof. Various peptide type raw materials are used for compounding the polypeptide repair liquid, so that the polypeptide repair liquid is rich in powerful repair ingredients; and acetyl hexapeptide-8, palmitoyl tripeptide-3, copper tripeptide-1, acetyl tetrapeptide-5, palmitoyl tetrapeptide-7 and carnosine are in combined action and synergistic interaction to dissolve and repair damaged cells in human bodies and promote a microcirculatory system to eliminate decomposed fat, so that the problem of poor cell protein repair is solved, and the purpose of repair is achieved. In addition, raw materials are rich in sources, so that the production cost is reduced, and the product competitiveness can be increased.

The invention provides a preparation method of quasipaa spinosa active peptide nutrition powder. The quasipaa spinosa active peptide nutrition powder comprises the following raw materials of quasipaaspinosa peptide powder and fruit powder. The preparation method of the quasipaa spinosa active peptide nutrition powder comprises the following steps of freezing sterilization, fishy flavor removal, grinding into thick liquid, enzymolysis, odor removal, spray drying and preparation of a finished product, wherein quasipaa spinosa is prepared into the peptide powder, and the peptide powder and the fruit powder are compounded to prepare the quasipaa spinosa active peptide nutrition powder. The active peptide nutrition powder comprises 16 amino acids and 20 trace elements or above, has high nutrition content, is rich in mineral substances, promotes osteocytogenesis, rapidly supplements calcium, can be directly absorbed by the intestinal tract, rapidly supplements active cells, increases antibodies, promotes the immune function and metabolism, is simple and convenient to eat, and can be directly drunk after being mixed with warm water or eaten after being stirred with supplementary food.

The invention relates to a drug for treating hyperlipidemia and a preparation method thereof. The drug is prepared by the following pharmaceutical raw materials: ginkgo leaf, hawthorn leaf, cassia seed, turmeric root-tuber, Mongolian snakegourd root, danshen root, sanchi, oriental waterplantain rhizome and prepared tuber fleeceflower root. The preparation steps thereof are as follows: 1) the sanchi is taken and smashed into fine powder, the ginkgo leaf and the hawthorn leaf are additionally taken and smashed into coarse powder, the percolation is carried out, percolate is collected, the recovery of ethanol with the reduced pressure is carried out, then the dilution, the removal of pigments and the separation of a water layer are carried out, ethyl acetate is used for shaking extraction, and the ethyl acetate is recovered with the reduced pressure from extract liquid and concentrated till the ethyl acetate is dry; 2) the cassia seed, the turmeric root-tuber, the Mongolian snakegourd root, the danshen root, the oriental waterplantain rhizome and the prepared tuber fleeceflower root are taken, 80 percent ethanol is used for reflux for two times, filtrate is merged, and the ethanol is recovered and vacuum-concentrated to a clear paste with the relative density of 1.40 and the temperature of 50 DEG C; and 3) the various medicine residues after the ethanol extraction are merged, then water is added for carrying out three times of decoction, decocted liquid is merged and filtered, then the standing is carried out, supernatant fluid is taken and concentrated to a thick paste, an extract of the step 2 and the sanchi powder are further added for even mixing, the mixture is dried at the temperature of 60 DEG C and then ground to the fine powder with 100 meshes, and the packaging into capsules with 0.5g is finally carried out for preparation.

The invention provides a pesticidal and bactericidal growth-promoting composition used for promoting the growth of crops. The pesticidal and bactericidal growth-promoting composition is a trace element fertilizer composed of the following components in percentages by weight: 30% to 50% of a component A, 40% to 60% of a component B and 5% to 10% of a component, wherein the component A is a plant-extracted pesticide which is any three selected from the group consisting of dried chinaberry seed powder, dried bitter gourd leaf powder, Pterocarya stenoptera leaf and bark powder, dried poisonous wolftooth-like bean powder and dried tobacco stem powder; the component B is a plant extracted bactericide which is any three selected from the group consisting of allicin powder, scutellaria bark powder, dried aloe powder, sophora alopecuroide powder and perilla plant powder; and the component C is any two selected from the group consisting of potassium, boron, calcium and zinc. The pesticidal and bactericidal growth-promoting composition provided by the invention is used for reinforcing disease-resistant and pest-resistant abilities of the crops, promotes the growth of the crops, greatly reduces the production cost of the crops through one-time application, and has significant effects of increasing yield and efficiency of rice and traditional Chinese medicines.

The invention relates to the field of mattresses in articles for daily use, in particular to an air-conditioning mattress. The air-conditioning mattress consists of an elastic body, and comprises a head part, a body part and a foot part, wherein the head part and the foot part consist of sponge bodies; the body part consists of an elastic cushion which comprises an air bag cushion layer and a bottom layer; the air bag cushion layer and the bottom layer have corresponding sizes, and the peripheries of the air bag cushion layer and the bottom layer are adhered together; an air supply pipe is arranged between the air bag cushion layer and the bottom layer; a plurality of air supply holes are uniformly formed on the air supply pipe; air outlet holes are formed among air bags of the air bag cushion layer; and gaps among the air bags of the air bag cushion layer form air supply passages. The mattress can supply cold and war air to ensure that a whole body feel comfortable, and the oxygen and the humidity of the body cannot volatilize when the mattress is used for a long time; besides, the mattress has the characteristics of fresh-keeping, deodorization and sterilization, can promote blood circulation, enhance antibodies, alleviate aching pains, and regulate blood pressure and autonomic nerves to achieve effects of beautification and the like, and has a physical therapeutic effect on various chronic diseases when used for a long time.

The invention discloses a DNA (deoxyribonucleic acid) level-based highly pathogenic blue-eared pig disease JEV (Japanese encephalitis virus) replicon vaccine and an application thereof. In the invention, primer amplification is carried out to obtain GP5 and M genes of a highly pathogenic blue-eared pig disease virus XH strain, an FMDV-2A sequence is inserted between the two genes by utilizing a fusion PCR (polymer chain reaction) method to obtain a G-2A-M segment, and finally the G-2A-M segment is cloned into pJEV-REP by utilizing two restriction enzyme cutting sites SpeI and SalI; besides, an IRES (internal ribosome entry site) sequence (G-2A-M-IRES) is inserted into the downstream of the G-2A-M segment, thus M protein can produce a real N terminal; and the G-2A-M-IRES segment is inserted into the pJEV-REP by utilizing two restriction enzyme cutting sites SalI-HF and SpeI, and finally a highly pathogenic blue-eared pig disease vaccine which is based on a JEV replicon and can express GP5 and M proteins is constructed.

HCV E1E2 compositions comprising E1E2 antigens, submicron oil-in-water emulsions and / or immunostimulatory nucleic acid sequences are described. The compositions can be used in methods of stimulating an immune response in a vertebrate subject.

The invention discloses a white goat breeding method in the field of animal husbandry breeding. The method comprises the following steps of sheep house construction, feed configuration, wherein feed is prepared from, by weight, 14-18 parts of corncob, 5-7 parts of mushroom residues, 6-9 parts of bran, 4-6 parts of bean pulp, 2-3 parts of mountain flour, 5-8 parts of soya residues and 10-15 parts of buckwheat, the raw materials are pulverized through a pulverizerand mixed, placed into boiling water to be boiled for 8-10 min, filtering is performed to obtain a mixture, 3-5 parts of vinegar withthe concentration being 4-5% is added into the mixture, then, uniform stirring is performed, standing is performed for 2-3 under the temperature of 45-50 DEG C, and 2-4 parts of salt is added for uniform stirring for use; fermentation liquid preparation; feed preparation, wherein the mixture and fermentation liquid are mixed and fermented for 4-5 d under the temperature of 60-70 DEG C, and then, acentrifugal dehydrator is used for dehydration, and a fermented material is obtained; feeding and management are performed; sheep pen disinfection. By adopting the white goat breeding method, nutrient substances can be supplied to white goat in time, and the meat quality of the white goat is greatly improved.

The present disclosure relates to a symmetric bispecific antibody of the class IgG4 comprising two heavy chains which each comprise a variable domain, CH1 domain and a hinge region, wherein in each heavy chain: the cysteine in the CH1 domain which forms an inter-chain disulphide bond with a cysteine in a light chain is substituted with another amino acid; and optionally one or more of the amino acids positioned in the upper hinge region is substituted with cysteine, wherein the constant region sequence of each heavy chain is similar or identical and the variable region in each heavy chain is different, formulations comprising the same, the use of each of the above in treatment and processes for preparing said antibodies and formulations.

The process of preparing multipurpose plant foliage fertilizer with natural botanic materials includes the following steps: spraying hot water to the surface of edible fungus, packing into plastic bag for some time, cutting into grains, soaking in hot water and regulating pH value, eliminating slag, adding sugar to obtain culture liquid; reacting the culture liquid in a reaction kettle via stirring at normal temperature, homogenizing in a homogenizer, and adding preservative and stabilizing agent to obtain foliage fertilizer. The present invention has the following advantages: high plant physiological activity of promoting and regulating plant metabolism and strengthening plant resistance and immunity, capacity of promoting plant absorption to organic components, etc.

HCV E1E2 compositions comprising E1E2 antigens, submicron oil-in-water emulsions and / or immunostimulatory nucleic acid sequences are described. The compositions can be used in methods of stimulating an immune response in a vertebrate subject.

The invention discloses a preparation method and application of virus Ankara subunit vaccine. The preparation method is characterized in that recombinant plasmid pFastBac HTB-Penton transforming DH10Bac E.coli is constructed through a baculovirus expression system so as to obtain correct recombinant bacmid Bacmid-Penton, then Sf9 cells are transfected to obtain an immunogenic gene Penton, Fiber 2 in which recombinant virus Ac-Penton expresses virus Ankara; the increased expression and purifying of protein are carried out; the protein expression quantity is large, and the protein activity is high; the protecting rate of Ankara subunit vaccine using Penton protein as antigen and Ankara mixed subunit vaccine using Penton+Fiber 2 protein as the antigen to virus Ankara reaches 100%, and the antibodies produced by the two vaccines are in high level and can be maintained for a long time; virus Ankara can be treated effectively by the two vaccines, so that huge economic loss of virus Ankara infection to the breeding industry can be decreased.

The invention belongs to the technical field of a traditional Chinese medicine composition for nourishing yin and raising yang, and particularly relates to a traditional Chinese medicine compositionfor raising yang, nourishing yin, invigorating blood circulation and nourishing skin. The traditional Chinese medicine composition for raising yang, nourishing yin, invigorating blood circulation andnourishing skin is prepared from the following raw materials of radix ginseng, radix salviae miltiorrhizae, radix codonopsis, rhizoma dioscoreae, cortex cinnamomic, poria cocos, radix astragali, herbataraxaci, lotus leaves, liquorice roots, prepared rehmannia roots, fructus arctii, rhizoma curcumae longae, sea cucumbers, radix ginseng rubra, hippophae rhamnoides, peach kernel, fructus psoraleae,spatholobus stems, eucommia barks, radix angelicae sinensis, roses, saffron crocus, coix seeds, wheat seedlings, radix paeoniae alba, rhizoma chuanxiong, fructus citrus junos, radix bupleuri and moringa seeds. Through the important combination in the scheme, the effects of strengthening the physique, improving immunity, purifying blood, enhancing antibodies, clearing summer-heat, eliminating dampness, raising lucid yang, warming the uterus and the stomach, nourishing the spleen, benefiting qi, and regulating medicine properties are achieved; a use method is simple; the effect taking speed is high; and side effects are small.

The invention discloses a high-efficiency chicken infectious bronchitis inactivated vaccine and a preparation method thereof. The virus content of every 0.3ml of the inactivated vaccine is not lower than (8.67+ / -0.81)*10<6>EID50 or not lower than (7.48+ / -0.45)*10<7> virus particle number. The preparation method comprises the following steps: inoculating a chicken infectious bronchitis virus to a chick embryo allantoic cavity, collecting the virus solution, concentrating the virus solution, measuring the content of the concentrated virus solution, inactivating by virtue of a formaldehyde solution, adding an adjuvant, emulsifying so as to prepare the inactivated vaccine. Because the high-efficiency chicken infectious bronchitis inactivated vaccine disclosed by the invention contains the concentrated virus solution, the content of antigens is improved, and the toxicity attack protection rate of a chicken infectious bronchitis breathing-type high-toxicity M41 strain is over 80 percent. Therefore, the inactivated vaccine disclosed by the invention can provide immunoprotection on the chicken infectious bronchitis strain, and the inactivated vaccine has good effects of preventing and controlling the chicken infectious bronchitis.

Hazelnut table vinegar is provided. The hazelnut table vinegar is a product obtained by adopting hazelnut kernel as a raw material and by steps of roasting, pulping, saccharifying, fermenting by alcohol and acetic acid, and sterilizing. Preparation of the hazelnut table vinegar includes steps of: treating the raw material, pulping, evaporating the raw material at normal pressure, performing size mixing, saccharifying, preparing a mixed liquor liquid, fermenting by alcohol, inoculating acetic bacteria, preparing grains and adding into a tank, fermenting by acetic acid, adding salt, curing, dipping and spraying, sterilizing, and clarifying to obtain a hazelnut table vinegar finished product. The hazelnut table vinegar is table vinegar and maintains original nutrients of hazelnut. The hazelnut table vinegar has functions of strengthening the spleen and the stomach, tonifying the liver, improving eyesight, boosting body activity functions, and the like, has high nutrition and an anti-ageing function, and is organic healthy table vinegar.

The invention discloses a bivalent inactivated vaccine for porcine reproductive and respiratory syndrome and a preparation method thereof. The bivalent inactivated vaccine for the porcine reproductive and respiratory syndrome comprises the following two inactivated viruses: a porcine reproductive and respiratory syndrome virus with the strain number of PRRSV-ZJ / H strain and the preservation number of CCTCC NO: V202130, and a porcine reproductive and respiratory syndrome virus with the strain number of PRRSV-ZJ / NB strain and the preservation number of CCTCC NO: V202140. The bivalent inactivated vaccine prepared by the invention can effectively prevent and control two major prevalent PRRS virus types in the current field, and a needle of the PRRS inactivated vaccine is immunized in a commercial pig field which is naturally infected or is immunized by using a PRRS attenuated live vaccine, so that the virus specificity neutralizing antibody can be obviously improved, the virus load in the infected pig body can be reduced, the PRRS epidemic situation can be quickly and effectively controlled, the daily gain of pigs can be obviously improved, and the economic benefits of pig farms are increased.

The invention discloses a live vaccine diluent as well as a preparation method and application thereof and a vaccine product, which relates to the field of vaccines. The live vaccine diluent disclosedby the invention is prepared from the following components: 0.01 to 1 percent of (W / V) Carbomer, 0.1 to 5percent of (W / V) levamisole as well as 0.0001 to 0.01 percent of (W / V) saponin. The live vaccine diluent can solve the problems that an animal live vaccine for immunizing swine fever, porcine pseudorabies and the like is susceptible to the infection of a maternal antibody, the titer of the antibody after the immunization is low, the production time is long, the maintenance time of the antibody is short and the like.