Lateral reciprocating immunochromatography method and diagnosis test strip and device based on lateral reciprocating immunochromatography method

Abstract

The invention discloses a lateral reciprocating immunochromatography method for detecting biological samples and a diagnosis test strip and a diagnosis device based on the method. The diagnosis device comprises a card bottom, a card cover and a diagnosis test strip, wherein the diagnosis test strip comprises a PVC (Polyvinyl Chloride) plate, and a sample layer, a mark layer, a coated film, a water absorbing layer, a buffer solution sample layer and a water-proof isolating film which are stuck to the PVC plate; the surface of the water absorbing layer is covered with the water-proof isolating film and the sample layer in sequence; the water-proof isolating film is easy to extract; the buffer solution sample layer, the mark layer, the coated film and the water absorbing layer are stuck in sequence along a horizontal extraction direction of the water-proof isolating film; the water absorbing layer and the coated film are not overlapped with each other; and the card cover is respectively provided with a liquid dripping window, an observation window and a sample window at positions corresponding to the buffer solution sample layer, the coated film and the sample layer respectively. The diagnosis device disclosed by the invention is applied to reciprocating immunochromatography, and an antigen or an antibody in a sample undergoes a secondary reaction with antibodies or antigens of T and C lines on the coated film, so that the antigen and antibody reaction time is increased, and the sensitivity is increased by 50-100 times than the conventional unidirectional chromatography test strip.

Description

technical field [0001] The invention relates to an immunological method, in particular to a lateral reciprocating immunochromatographic method and a diagnostic test strip and device based on the method. Background technique [0002] In the past 20 years, the principle of immunochromatography has been widely used in the field of clinical rapid diagnosis and detection, especially the colloidal gold diagnostic test strip, which can be tested for a single person or multiple people at the same time, which is simple and fast without any equipment , the result can be judged by naked eyes within a few minutes, and the reagent can be stored at room temperature for up to two years, which has great advantages in in vitro screening and bedside inspection. [0003] By the end of 2010, there were more than 100 varieties of colloidal gold immunochromatography reagents, involving more than a dozen fields such as infectious diseases, tumor markers, prenatal and postnatal care, drug testing, ...

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