31results about How to "Increase binding rate" patented technology

The invention discloses a non-natural amino acid as well as a preparation method and application thereof. The novel non-natural amino acid is obtained by introducing long and soft side chain amino acid into a classical ASF side chain for the first time, the stability of the obtained non-natural amino acid is enhanced, the reaction radius of the non-natural amino acid is increased, the non-natural amino acid can touch amino acid residues with farther distance, and the reactivity is improved. In addition, compared with other covalent amino acids, the covalent amino acids have higher reaction rate and binding efficiency. The polypeptide and related drugs obtained based on the non-natural amino acid can be high in safety, can cross a blood brain barrier to realize brain targeting, and have better anti-tumor activity, so that the polypeptide and related drugs have extremely high medical value.

The invention relates to the technical field of cylinder sleeves and detection thereof, in particular to a burr-shaped cast-in type cylinder sleeve and a detection method thereof. The cylinder sleeve is made of cast iron and is cast into the cylinder body through a die-casting or gravity casting process, and a plurality of burrs are formed on the peripheral face of the cylinder sleeve. At least one burr meets the following conditions: in the burr-shaped cast-in cylinder sleeve, the diameter of the burr at the position 0.25 mm away from the top end is L1, and the diameter of the burr at the position 0.4 mm away from the top end is L2; and the ratio of the diameter L1 to the diameter L2 is more than or equal to 0.65 and less than or equal to 1.15. The specific shapes of the burrs are limited by limiting the diameter sizes of the two special positions, away from the top end, of the burrs, so that the binding rate between the single burrs and the cylinder body and the binding rate between the cylinder sleeve and the cylinder body are guaranteed, good heat dissipation performance between the cylinder sleeve and the cylinder body is guaranteed, and it is guaranteed that the cylinder sleeve does not deform in the working process.

The present invention provides a device and method for treating a water body, comprising a membrane biological reaction cylinder, on which a plurality of membrane biological reaction units are arranged in an annular array, and the plurality of membrane biological reaction units are connected with the inside of the membrane biological reaction cylinder through water inlet holes arranged at the connection of the membrane biological reaction cylinder, the purification cylinder body is formed by a pair of water inlet ducts and a pair of arc plates fixedly welded, the plurality of combing aeration rods are respectively connected with corresponding aeration cylinders through air channels arranged inside the arc plates, the ends of the telescopic rods away from the pistons are movably sleeved on the camshafts, and combing gaps are formed between the plurality of L-shaped connecting rods, the membrane biological reaction units of the present invention are in a rotating state, and cooperate with the direct aeration and combing of the combing aeration rods, thereby increasing the probability of combining biofilm fillers with oxygen, effectively preventing particulate matter from adhering to MBR hollow fiber filaments, and thus ensuring the purification efficiency of the membrane biological reaction unit.

The application belongs to the field of biomedical application, and more particularly relates to a cell fluorescence probe anchoring cell integrin, and a preparation method and application thereof. The cell fluorescence probe comprises a polypeptide complex, a fluorescent molecule and a cyclodextrin unit, the polypeptide complex comprises a cysteine-containing peptide, a peptide containing a bonding group and a targeting peptide which are connected in any order, the cyclodextrin unit contains a carbon-carbon double bond functional group, and is combined with the cysteine-containing peptide through a click reaction to form the polypeptide complex, the polypeptide complex is covalently connected to the fluorescent molecule through the bonding group, and the fluorescent molecule is an aggregation-induced emission compound. The double bond functional group of the modified cyclodextrin is bonded to the sulfhydryl group of the terminal cysteine of the polypeptide through a click reaction, and the sulfhydryl group of the terminal cysteine of the polypeptide is bonded to the double bond functional group of the modified cyclodextrin through a click reaction, so that the cyclodextrin is improved to protect the fluorescent molecule and prolong the fluorescent time effectiveness of the fluorescent imaging reagent.

The invention discloses a raw material extraction device for medicine for treating fatty liver. The raw material extraction device comprises a balance frame, an extraction barrel, a servo motor, a conical heating disc, a filter screen, a pre-cooling disc, a refrigeration tank, a rotating barrel, a rotating shaft, a defoaming assembly, a circulating opening and closing assembly and a purification assembly. The extraction cylinder is fixedly installed on the upper end face of the balancing frame, the servo motor is fixedly installed at the upper end of the extraction cylinder, the conical heating disc is fixed in the extraction cylinder in a sealed mode, the filter screen is fixed in the extraction cylinder and located below the extraction cylinder, and the pre-cooling discs are fixed in the extraction cylinder and located below the filter screen. The conical heating disc, the filter screen and the pre-cooling disc are fixedly installed on the outer wall of the extraction barrel respectively, the rotating barrel is fixed to the output end of the servo motor, the rotating shaft sequentially and rotationally penetrates through the conical heating disc, the filter screen and the pre-cooling disc to be arranged on the inner wall of the lower end of the extraction barrel, the defoaming assembly is arranged above the conical heating disc, and the circulating opening and closing assembly is arranged between the conical heating disc and the filter screen. And the purification assembly is arranged between the plurality of refrigeration tanks and the extraction barrel.

The invention discloses a preparation method of a Salmonella typhimurium aptamer and a colorimetric probe thereof, and belongs to the technical field of food safety. The DNA sequence of the Salmonella typhimurium aptamer is shown in SEQ ID No: 1. The steps of using the Salmonella typhimurium aptamer for the preparation of the colorimetric probe are as follows: (1) preparing a gold nanoparticle solution with tetrachloroauric acid solution and distilled water; (2) preparing a Salmonella typhimurium aptamer solution with distilled water; (3) mixing the gold nanoparticle solution and the Salmonella typhimurium aptamer solution in a volume ratio of 5:1.8 to prepare a colorimetric probe solution; the ultraviolet absorption wavelength of the gold nanoparticles modified by the nucleic acid aptamer in the colorimetric probe solution is 523nm. It has been verified that compared with the existing mature nucleic acid aptamers, the colorimetric probe prepared by the invention has a higher linear fitting degree and a lower detection limit; at the same time, the screening method is simple and convenient, and rapid screening can be achieved by only building a simple server.

The application relates to an immune analysis micro-fluidic chip, which comprises a chip body, the chip body defining an antibody reconstitution flow channel, a fixing area and a waste pool in sequence, the antibody reconstitution flow channel comprising first and second annular flow channels arranged alternately, the first and second annular flow channels being opposite in opening direction, the first annular flow channel having a circumferential angle of alpha between the two ends along the extension direction, the second annular flow channel having a circumferential angle of beta between the two ends along the extension direction, and alpha and beta satisfying 180 DEG < alpha <= 270 DEG and 180 DEG < beta <= 270 DEG. According to the immune analysis micro-fluidic chip, the reconstitution rate of fluorescent antibodies can be improved, and the combination rate of the fluorescent antibodies and antigens in samples can be improved.

The present invention discloses a method for improving the stability and bioavailability of folic acid and its application. It belongs to the technical field of natural folic acid products. The method for improving the stability and bioavailability of folic acid is achieved by mixing folic acid with folic acid binding protein, adding a citric acid solution with a pH of 4.3 to 5 and 0.4M, sealing, and treating with 200 to 450MPa ultra-high static pressure for 2 to 20 minutes. The present invention uses ultra-high static pressure to modify folic acid binding protein, and then combines natural folic acid to form a natural folic acid-folate binding protein nutrient. The product has high stability and bioavailability and can be used as a food ingredient in functional foods, special medical foods and food supplements.

The application discloses a preparation method of Y molecular sieve, which comprises the following steps: mixing a silicon source, an aluminum source, an alkali source and a template agent to form a molecular sieve mother liquor, and performing crystallization, filtration, washing, drying and calcination to obtain the Y molecular sieve, wherein the template agent is polydialkyldimethylammonium chloride. The Y molecular sieve synthesis method is simple, the template agent used is environment-friendly and low in price, and the Y molecular sieve prepared by the method has the advantages of high silicon-aluminum ratio, large specific surface area, high crystallinity and good mass transfer effect.

The utility model discloses a spraying system of a spraying tower, which relates to the waste gas treatment technology, and adopts the technical scheme that the spraying system comprises a tower body, a spraying pipe and a nozzle, the spraying pipe comprises a main pipe and a branch pipe, the nozzle is rotatably connected to the branch pipe, a rotating piece is arranged in the nozzle, and the nozzle is provided with an atomizing opening and a liquid flow opening. The spray head starts to rotate under the action of the rotating part, the treatment liquid in the spray head is sprayed out from the atomization opening and the liquid flow opening under the action of liquid pressure, the treatment liquid sprayed out from the liquid flow opening is large in particle diameter and can effectively combine pollutants with large diameters in waste gas, the treatment liquid sprayed out from the atomization opening is small in particle diameter, and the pollutants can be effectively combined with the pollutants with large diameters in the waste gas. The spray tower is simple in structure, large in dispersion area and longer in hang time, and can be fully combined with pollutants with smaller diameters in waste gas, and meanwhile, as the rotation of the spray head can provide centrifugal force for the treatment liquid leaving the spray head, the treatment liquid sprayed out of the atomization port and the liquid flow port can have wider coverage area, and the working efficiency of the spray tower is improved.

The application belongs to the technical field of brush materials, and provides a silver graphite brush material and a preparation method thereof.The raw materials of the silver graphite brush material include flake graphite particles, silver powder and nano silicon powder.The silver graphite brush material breaks the technical limitations of traditional resin bonding systems and homogeneous structures, can take into account the advantages of original wear resistance, high temperature resistance and long service life, and can also solve the industry difficulties of poor assembly welding reliability of small brushes and high environmental protection cost.The preparation method can realize synchronous improvement of micro interfaces and macro structures, and the process is stable, controllable and industrialized batch production.

The invention discloses saccharomyces cerevisiae for relieving heme feedback inhibition and strengthening heme supply and application, and belongs to the technical field of genetic engineering. The invention constructs a 5-aminolevulinic acid synthetase mutant capable of removing heme, and the yield of 5-aminolevulinic acid and the yield of heme of a strain H5 constructed by in-situ integration of the mutant in saccharomyces cerevisiae can be increased by 1.69 times and 2.99 times respectively compared with a control strain CEN.PK2-1C. According to the present invention, the HEM1I213K and the HEM13 are further integrated on the basis of the H5 strain in the multi-copy manner, such that the heme synthesis of the obtained heme-reinforced strain H25 is improved by 16.57 times compared with the CEN.PK2-1C, the yield of the synthesized rHb and the heme binding rate are improved by 22.9% and 39.2% compared with the CEN.PK2-1C added with 25 mg / L heme, and the heme supply-reinforced and high-activity human hemoglobin synthesis can be easily achieved.

The invention provides a composite preparation process of a multi-layer adsorption material, and belongs to the technical field of multi-layer adsorption preparation.The composite preparation process of the multi-layer adsorption material comprises the following steps that grey cloth is placed through a bearing mechanism so that the grey cloth can be conveniently received; stirring of the adsorption material and the adhesive is realized through the stirring mechanism, so that the adsorption material and the adhesive are conveniently mixed; the mixed materials are scattered to gray cloth through the material scattering mechanism, so that the gray cloth and the adsorption materials form a composite fabric; power is provided through the driving mechanism, so that the operation of the stirring mechanism and the spreading mechanism is conveniently controlled; according to the hot-pressing compounding device, through the arrangement of the material scattering mechanism, the multiple spraying heads can be driven to move, so that an adsorption material sprayed to gray cloth can be more uniform and is not prone to being stacked together, and hot-pressing compounding of the gray cloth and the adsorption material can be better achieved.

This invention discloses a flow cytometry method for detecting fluorescent microspheres based on TSA signal amplification. The method includes: incubating fluorescently encoded microspheres coated with a capture antibody with a sample; adding a biotinylated detection antibody to form a sandwich complex; subsequently introducing two high-density signal amplification polymers sequentially: SA-PolyHRP400 as the first signal amplification polymer, and a second signal amplification polymer with a polysaccharide backbone and covalently coupled with multiple tyrosine and luciferin molecules. Under HRP catalysis, the tyrosine is activated and covalently deposited in the vicinity of the microspheres, achieving exponential amplification of the fluorescence signal. This method is simple to operate, more efficient than traditional methods, and more suitable for high-throughput, high-timeliness platforms like flow cytometry. It also exhibits high sensitivity, high specificity, and good repeatability for detecting low-abundance proteins such as IL-6 based on the sandwich method, and is compatible with non-magnetic / magnetic fluorescently encoded microspheres of various sizes. It is suitable for multi-indicator detection and can be widely applied to early clinical diagnosis and trace biomarker analysis.

The invention relates to the technical field of continuous coating of steel strips, and particularly discloses a manufacturing method of a corrosion-resistant pre-coating film steel strip and a pre-coating film hot-dip coating layer steel strip resistant to CX-grade extreme environment corrosion. According to the invention, the preparation method of the pre-coating hot-dip coating steel strip is optimized, and the pre-coating hot-dip coating steel strip prepared according to the method disclosed by the invention can sufficiently resist corrosion even in a CX-grade corrosion environment due to the synergistic cooperation of the alloy coating primer layer, the alloy coating finish paint layer and the passivation layer generated in passivation treatment; and the service life is longer and the replacement frequency is lower, so that the defects in the related technology are overcome.

The present invention discloses a shearing type mineralization device, which comprises the following components: a pump (1), a dispersion component (2), a storage component (3), which comprises a storage chamber (31), a second mounting plate (32) and a storage chamber outlet (33); the second mounting plate (32) is provided with a recessed dispersion component accommodating area (323) and a non-recessed annular platform (321), the latter having a plurality of curved shear baffles (322) and a material throwing trough (324), the material throwing trough (324) being fluidly connected to the storage chamber (31) through a material flow hole (325) downstream; and a feed port (6). The device of the present invention can not only fully mix the coal slurry and the mineralizing agent, but also can shear the air in the device into micron-sized bubbles, thereby increasing the combination rate of carbon-hydrogen-containing combustible particles and bubbles, the phase interface obtained after the mineralized material of the present invention is statically layered, and the separation effect of carbon-hydrogen-containing combustible particles, mineral particles and water is good.

The application relates to a CMP composition for obtaining a high-quality SiC single crystal substrate surface, which is prepared from the following raw materials in percentage by weight: 20-50 wt% of polishing abrasive, 3-5 wt% of oxidizing agent, 0.1-0.5 wt% of surfactant, 0.2-0.5 wt% of hydrogen bond regulator, 0.2-1 wt% of polishing accelerator, 0.01-0.05 wt% of defoaming agent, and the balance of water; the CMP composition is neutral. The CMP composition uses silicon oxide as the abrasive, introduces the hydrogen bond regulator, the polishing accelerator and the oxidizing agent, improves the polishing rate of the silicon carbide wafer CMP, realizes that the surface roughness Ra of the silicon surface after cleaning can reach below 0.07 nm, the surface after cleaning is free of large particles (>0.2 um), meanwhile, the silicon oxide raw material does not need to be treated, has excellent long-term storage stability, and the system does not have the phenomena of particle agglomeration and sedimentation with the prolongation of the storage time, and excellent polishing surface quality can be obtained in the subsequent polishing process.

The invention relates to the technical field of sputtering target material binding, in particular to a binding method of a rotating molybdenum target material. The binding method of the rotating molybdenum target material comprises the following steps of back pipe treatment, rotating molybdenum target treatment, rotating target material installation, rotating target material assembly and rotating target material binding. According to the method, the back tube and the rotating molybdenum target are subjected to roughening treatment twice and ultra-indium alternate treatment twice, so that alloy impurities are reduced, the bonding strength between the target and the backing tube is improved, the binding rate and the overall reliability of the target are improved, and the service life of the target is prolonged while it is guaranteed that the target and the back tube have good adhesion performance. And the binding rate of the target material is up to 98% or above.

The invention discloses a hemoglobin mutant with improved stability and application thereof, and belongs to the technical field of gene engineering. According to the invention, a high-throughput screening method for structural stability of human hemoglobin and heme binding reinforcement mutants is designed, so that a foundation is laid for obtaining ideal stable human hemoglobin mutants. Hot amino acid sites for structural stability and thermal stability modification of human hemoglobin are determined through semi-rational design, iterative saturated mutation is further performed to obtain an ideal mutant rHb9th, and the mutant rHb9th has the advantages of structural stability, thermal stability, autoxidation resistance, heme binding rate and the like. Compared with the rHbwild-type, the anti-heme loss rate, the yield of human hemoglobin and the like are remarkably improved.

The invention discloses a phage display library panning method based on elastic tag-like recombinant protein, and belongs to the technical field of biology. According to the method, the reversible phase change characteristic of elastin-like proteins (ELPs) is utilized, a target antigen sequence used for elutriation is fused with the ELPs, an antibody of an anti-specific antigen sequence displayed by phage is elutriated from a solution through multiple reversible phase change cycles, that is, the antibody displayed by the phage and combined with the specific antigen can be obtained through enrichment through multiple rounds of elutriation and amplification, and the specific antigen sequence of the ELPs is detected. The process only needs a conventional centrifugal machine and a constant-temperature water bath kettle, has the advantages of low cost, simplicity and convenience in operation and the like, and provides a new idea for elutriation of the phage display library.

The present invention relates to a preparation method of hydroxypropyl methylcellulose ether for neutral putty for interior walls, and belongs to the technical field of cellulose ether building materials. The method comprises the following steps: mixing a hydrophilic modified graphene dispersion with a sodium polycarboxylate liquid dispersant; S2, spraying the liquid obtained by mixing the hydrophilic modified graphene dispersion with the sodium polycarboxylate liquid dispersant on the surface of the HPMC gel material after filter pressing in the HPMC granulation stage; granulating the gel material, crushing at low temperature, and obtaining a modified HPMC powder; and physically mixing the modified HPMC powder with a water reducer, pregelatinized corn starch, and polyethylene glycol through an air flow mixer according to a formula to obtain the hydroxypropyl methylcellulose ether for neutral putty for interior walls. The present invention effectively isolates the viscous material by relying on the material's own effect, improves the smoothness of the scraping process, and realizes efficient linear motion conversion, thereby improving the construction performance.

The invention provides a welding method of a nickel-aluminum alloy target material assembly, which comprises the following steps: arranging a chromium-aluminum layer on the welding surface of a nickel-aluminum alloy target material, then assembling the nickel-aluminum alloy target material and a back plate, putting the nickel-aluminum alloy target material and the back plate into a sheath, and then sequentially carrying out degassing and hot isostatic pressing welding; and the molar ratio of the chromium element to the aluminum element in the chromium-aluminum layer is (0.4-0.6): 1. The specific plating layer is arranged on the welding surface of the nickel-aluminum alloy target material, and subsequent hot isostatic pressing welding is combined, so that the obtained nickel-aluminum alloy target material assembly does not have any deformation, and meanwhile, high-strength and high-binding-rate welding of the nickel-aluminum alloy target material and the back plate is realized. The welding method is simple in process, efficient and suitable for batch production.

The invention belongs to the technical field of soft magnetic materials, and discloses a CoFeV alloy target material and a preparation method thereof. The preparation method comprises the following steps: carrying out vacuum induction melting and refining on a Co sheet, a Fe sheet and a V sheet, and then carrying out casting molding to obtain a CoFeV alloy ingot; the CoFeV alloy cast ingot is subjected to hot rolling treatment; a rough blank is obtained; performing water cutting on the rough blank to obtain a target blank; and the obtained target blank is machined through a grinding machine, the target blank with the flat and bright surface is obtained, finally, the target blank is welded to a copper back plate, and the CoFeV alloy target material is obtained. According to the preparation method, the CoFeV alloy target material is prepared through vacuum melting, and the preparation process conditions are further optimized, so that the quality of the CoFeV alloy target material can be remarkably improved, and the bonding rate of welding with a copper back plate is improved.

The invention relates to the technical field of coating, in particular to a soldering flux coating device for soldering tin sheets, which comprises a coating cylinder, a driving mechanism and an extrusion mechanism, a rotary column is arranged at the rear end of the coating cylinder; a plurality of atomizing nozzles which are uniformly distributed in the circumferential direction of the rotating column are arranged at the front end of the rotating column; an annular groove is formed in the circumferential side wall of the rotating column; and hoses are arranged in the groove front and back. When the atomization nozzles are blocked, the extrusion mechanism clamps and extrudes the hose and drives the high-temperature soldering flux liquid in the hose to wriggle towards the atomization nozzles, so that the liquid amount of the soldering flux liquid conveyed to each atomization nozzle is the same, and when the atomization nozzles are blocked, the extrusion mechanism clamps and extrudes the hose towards the atomization nozzles, so that the hydraulic pressure of the soldering flux liquid in the atomization nozzles is increased; the high-temperature soldering flux liquid assists the solidified soldering flux in the atomizing nozzles to be melted again, so that the high-pressure soldering flux liquid dredges the atomizing nozzles, the spraying amount of the soldering flux at each atomizing nozzle is uniform and consistent, and the soldering flux coating effect is improved.

The invention belongs to the technical field of copper-clad aluminum wire manufacturing, and discloses a high-interface bonding rate copper-clad aluminum wire forming process which comprises the following steps: S1, raw material preparation and pretreatment: a, aluminum rod and copper strip preparation: processing and slitting an aluminum rod and a copper strip; the bonding strength and quality between the copper layer and the aluminum core are remarkably improved through electric field assisted bonding treatment, the diffusion process between copper atoms and aluminum atoms can be accelerated through the electric field, bonding between the copper layer and the aluminum core is tighter, and the effect of the electric field is similar to a driving force; according to the copper-clad aluminum wire forming process, atoms can cross an energy barrier more easily under the action of electric field force, so that more efficient combination is achieved, interaction force between the atoms is enhanced under the action of an electric field, the interface bonding force is improved, and the copper-clad aluminum wire forming process under the assistance of the electric field can remarkably improve the bonding strength between a copper layer and an aluminum core; therefore, the high interface bonding rate is greatly improved, and the interface bonding between the copper layer and the aluminum core is firmer and more stable.

The application discloses an EMT molecular sieve and a preparation method and application thereof. The method comprises the following steps: (1) preparation of a directing agent: the molar ratio of Na2O, Al2O3, SiO2 and H2O in the directing agent is (16-19):1:(4-6):(200-400), preferably 17-18:1:4-6:250-350; (2) synthesis of the molecular sieve: the silicon source, the aluminum source, the alkali source and water are uniformly mixed with the directing agent obtained in the step (1), and then crystallization, filtration, washing, drying and calcination are performed to obtain the EMT molecular sieve. The method is used for synthesizing the EMT molecular sieve by the directing agent method for the first time.

The application discloses a molecule for improving hair toughness and application thereof. The molecule for improving hair toughness is a molecule with conjugated double bonds obtained through esterification or acylation reaction of a compound with two hydroxyl groups at two ends, a compound with two amino groups at two ends or a compound with a hydroxyl group at one end and an amino group at the other end and an organic acid or an acid anhydride containing an alpha,beta-carbon-carbon double bond, and the molecule is used to improve hair toughness through Michael addition reaction with sulfydryl in damaged hair. The molecule for improving hair toughness prepared in the application is combined with sulfydryl through a covalent bond, improves the mechanical properties of hair, has relatively good permeability, and can avoid further oxidation of free sulfydryl.

The invention belongs to the technical field of high-precision grinding machining of mandrels of large extrusion granulation units, and discloses a novel involute spline mandrel fan-shaped supporting device and a using method thereof. Locking pressing plates are installed on the two sides of the bottom of the fan-shaped supporting body and located on the sliding rails. The side supporting adjusting device adjusts the telescopic amount of the side supporting column. The lower supporting adjusting device adjusts the telescopic amount of the lower supporting column. The other end of the lower supporting column is connected with a fan-shaped supporting tile body; the fan-shaped supporting tile body is made of nylon; the fan-shaped arc of the fan-shaped supporting tile body is in an interval; one end of the spherical positioning rod is connected with the fan-shaped supporting tile body, and the other end of the spherical positioning rod is clamped in two semicircular grooves in the 10-degree inclined face of the front end of the side supporting column. The problem that high-precision and stable machining supporting cannot be achieved after hobbing of the high-precision involute spline mandrel is solved, the high-precision grinding machining effect is achieved, and the high-precision grinding machining problem of high-precision involute spline parts is comprehensively solved.

The invention discloses a multifunctional nutritional feed capable of improving meat quality of native chickens and a preparation method thereof, soybean meal and fish meal are respectively prepared into macromolecular protein and micromolecular protein, the macromolecular protein and the micromolecular protein are combined and then added and polymerized to form microcapsule feed, and animal protein and plant protein are combined to form the good multifunctional nutritional feed. The microcapsule formed by combining the large protein and the small protein can meet the requirements of easy absorbability and decomposition resistance at the same time, can have decomposition resistance in the esophagus and can be fully absorbed by the digestive tract, and the defect that in the prior art, the bioavailability of feed is low is overcome; a hydroxylation reagent is added into a small-molecule solution, so that small-molecule protein is connected with a plurality of hydroxyl functional groups and is negatively charged, agglomeration among small molecules can be effectively avoided, the small-molecule protein can be combined with macromolecular protein with high surface carboxyl content, and the binding rate of the macromolecular protein and the small-molecule protein can be increased; the integrity of the feed is improved.

The present invention discloses a multi-stage mixing device and method for solid fuel and liquid medium, the device comprising: an inner sleeve (1); an outer sleeve (2), the inner sleeve (1) and the outer sleeve (2) being connected and fixed by a bracket (9); a solid fuel slurry inlet (41), the solid fuel slurry inlet (41) being coaxial with the inner sleeve (1); an air inlet (42); a micro-nano bubble generating component (5); and a material outlet (71). The multi-stage mixing device of the present invention can pre-mineralize solid fuel particle materials separately before a flotation separation device, thereby improving the mineralization degree of solid fuel particles. The higher the mineralization degree, the better the flotation separation effect in the subsequent foam flotation tower, which is reflected in the more obvious phase interface between the foam phase and the water phase in the foam flotation tower, and also avoids the problem of a large number of mineral particles being mixed in the carbon-hydrogen combustible particles in a single tank flotation device. The multi-stage mixing device of the present invention can also be used to prepare a suspended stable liquid-like fuel.