6results about How to "Increase binding rate" patented technology

The application belongs to the field of biomedical application, and more particularly relates to a cell fluorescence probe anchoring cell integrin, and a preparation method and application thereof. The cell fluorescence probe comprises a polypeptide complex, a fluorescent molecule and a cyclodextrin unit, the polypeptide complex comprises a cysteine-containing peptide, a peptide containing a bonding group and a targeting peptide which are connected in any order, the cyclodextrin unit contains a carbon-carbon double bond functional group, and is combined with the cysteine-containing peptide through a click reaction to form the polypeptide complex, the polypeptide complex is covalently connected to the fluorescent molecule through the bonding group, and the fluorescent molecule is an aggregation-induced emission compound. The double bond functional group of the modified cyclodextrin is bonded to the sulfhydryl group of the terminal cysteine of the polypeptide through a click reaction, and the sulfhydryl group of the terminal cysteine of the polypeptide is bonded to the double bond functional group of the modified cyclodextrin through a click reaction, so that the cyclodextrin is improved to protect the fluorescent molecule and prolong the fluorescent time effectiveness of the fluorescent imaging reagent.

The application relates to an immune analysis micro-fluidic chip, which comprises a chip body, the chip body defining an antibody reconstitution flow channel, a fixing area and a waste pool in sequence, the antibody reconstitution flow channel comprising first and second annular flow channels arranged alternately, the first and second annular flow channels being opposite in opening direction, the first annular flow channel having a circumferential angle of alpha between the two ends along the extension direction, the second annular flow channel having a circumferential angle of beta between the two ends along the extension direction, and alpha and beta satisfying 180 DEG < alpha <= 270 DEG and 180 DEG < beta <= 270 DEG. According to the immune analysis micro-fluidic chip, the reconstitution rate of fluorescent antibodies can be improved, and the combination rate of the fluorescent antibodies and antigens in samples can be improved.

The utility model discloses a spraying system of a spraying tower, which relates to the waste gas treatment technology, and adopts the technical scheme that the spraying system comprises a tower body, a spraying pipe and a nozzle, the spraying pipe comprises a main pipe and a branch pipe, the nozzle is rotatably connected to the branch pipe, a rotating piece is arranged in the nozzle, and the nozzle is provided with an atomizing opening and a liquid flow opening. The spray head starts to rotate under the action of the rotating part, the treatment liquid in the spray head is sprayed out from the atomization opening and the liquid flow opening under the action of liquid pressure, the treatment liquid sprayed out from the liquid flow opening is large in particle diameter and can effectively combine pollutants with large diameters in waste gas, the treatment liquid sprayed out from the atomization opening is small in particle diameter, and the pollutants can be effectively combined with the pollutants with large diameters in the waste gas. The spray tower is simple in structure, large in dispersion area and longer in hang time, and can be fully combined with pollutants with smaller diameters in waste gas, and meanwhile, as the rotation of the spray head can provide centrifugal force for the treatment liquid leaving the spray head, the treatment liquid sprayed out of the atomization port and the liquid flow port can have wider coverage area, and the working efficiency of the spray tower is improved.

This invention discloses a flow cytometry method for detecting fluorescent microspheres based on TSA signal amplification. The method includes: incubating fluorescently encoded microspheres coated with a capture antibody with a sample; adding a biotinylated detection antibody to form a sandwich complex; subsequently introducing two high-density signal amplification polymers sequentially: SA-PolyHRP400 as the first signal amplification polymer, and a second signal amplification polymer with a polysaccharide backbone and covalently coupled with multiple tyrosine and luciferin molecules. Under HRP catalysis, the tyrosine is activated and covalently deposited in the vicinity of the microspheres, achieving exponential amplification of the fluorescence signal. This method is simple to operate, more efficient than traditional methods, and more suitable for high-throughput, high-timeliness platforms like flow cytometry. It also exhibits high sensitivity, high specificity, and good repeatability for detecting low-abundance proteins such as IL-6 based on the sandwich method, and is compatible with non-magnetic / magnetic fluorescently encoded microspheres of various sizes. It is suitable for multi-indicator detection and can be widely applied to early clinical diagnosis and trace biomarker analysis.

The application relates to a CMP composition for obtaining a high-quality SiC single crystal substrate surface, which is prepared from the following raw materials in percentage by weight: 20-50 wt% of polishing abrasive, 3-5 wt% of oxidizing agent, 0.1-0.5 wt% of surfactant, 0.2-0.5 wt% of hydrogen bond regulator, 0.2-1 wt% of polishing accelerator, 0.01-0.05 wt% of defoaming agent, and the balance of water; the CMP composition is neutral. The CMP composition uses silicon oxide as the abrasive, introduces the hydrogen bond regulator, the polishing accelerator and the oxidizing agent, improves the polishing rate of the silicon carbide wafer CMP, realizes that the surface roughness Ra of the silicon surface after cleaning can reach below 0.07 nm, the surface after cleaning is free of large particles (>0.2 um), meanwhile, the silicon oxide raw material does not need to be treated, has excellent long-term storage stability, and the system does not have the phenomena of particle agglomeration and sedimentation with the prolongation of the storage time, and excellent polishing surface quality can be obtained in the subsequent polishing process.

The application discloses a molecule for improving hair toughness and application thereof. The molecule for improving hair toughness is a molecule with conjugated double bonds obtained through esterification or acylation reaction of a compound with two hydroxyl groups at two ends, a compound with two amino groups at two ends or a compound with a hydroxyl group at one end and an amino group at the other end and an organic acid or an acid anhydride containing an alpha,beta-carbon-carbon double bond, and the molecule is used to improve hair toughness through Michael addition reaction with sulfydryl in damaged hair. The molecule for improving hair toughness prepared in the application is combined with sulfydryl through a covalent bond, improves the mechanical properties of hair, has relatively good permeability, and can avoid further oxidation of free sulfydryl.