Anti-PD-1 monoclonal antibody and application thereof

A monoclonal antibody, PD-1 technology, applied in the fields of application, antibody, anti-tumor drugs, etc., can solve the problems of ineffective activation of the immune system and immune escape of tumor cells, and achieve the recovery of lymphocyte activity, high binding rate, and specificity good sex effect
CN108314734AActive Publication Date: 2018-07-24CHINA PHARM UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHINA PHARM UNIV
Publication Date
2018-07-24

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Abstract

The invention relates to a novel anti-PD-1 monoclonal antibody and a variable region sequence of the antibody and belongs to the technical field of biology. The novel anti-PD-1 monoclonal antibody takes recombinant human PD-1 protein (rhEPD-1) as an immunogen for immunizing a BAL b / c mouse; spleen cells of the mouse are taken and are fused with myeloma cells sp2 / 0-Ag14 to obtain a hybridoma cell line capable of expressing the anti-PD-1 antibody; the cell line can stably secrete the single monoclonal antibody. According to the novel anti-PD-1 monoclonal antibody, variable region nucleotide andamino acid sequences of an H chain and an L chain of the anti-PD-1 monoclonal antibody are cloned at the same time. The anti-PD-1 monoclonal antibody provided by the invention can be specifically combined with PD-1 and the combination of the PD1 and PD-L1 is blocked; functions of T cells are recovered. The novel anti-PD-1 monoclonal antibody can be used as an immune checkpoint inhibitor and is used for treating cancer and infectious diseases.
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Description

Technical field:

[0001] The invention relates to a monoclonal antibody that can specifically bind to PD-1, and belongs to the field of biotechnology. Specifically, it relates to the preparation method of the monoclonal antibody, its variable region sequence and its application. Background technique:

[0002] Hybridoma technique (hybridoma technique), also known as monoclonal antibody technique, in 1975 Koehler and Milstein proved that the fusion of myeloma cells with immunized animal spleen cells can form highly specific monoclonal antibodies against a certain antigen. Hybridoma technology utilizes the characteristics that myeloma cells can be serially passaged in vitro and that lymphocytes can secrete specific antibodies or factors. When two kinds of cells are fused, the successfully fused cells can have the main characteristics of these two kinds of cells.

[0003] PD-1 / PD-L1 immunotherapy improves the immune response by blocking the interaction between the two, and has t...

Claims

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