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Kit for determining serum amyloid protein A and preparation method thereof
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A technology of serum starch and kits, which is applied in the field of medical immunodiagnosis, and can solve the problems of cumbersome operations, fewer kits, and low product sensitivity
Inactive Publication Date: 2017-08-04
SHENZHEN HUISONG TECH DEV
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Problems solved by technology
At present, there are very few kits for the determination of serum amyloid A, which have not been popularized and applied. At the same time, other methods also have the characteristics of cumbersome operation, low product sensitivity and product instability.
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[0075] Preparation method of serum amyloid A assay kit
[0076] In the present invention, the preparation method of the assay kit of serum amyloid A comprises the steps:
[0077] (A) prepare each component of R1 reagent, make R1 reagent;
[0078] (B) Prepare SAA antibody latex microspheres and other components of the R2 reagent to obtain the R2 reagent.
[0079] Wherein, the SAA antibody latex microspheres are prepared through the following steps:
[0080] (a) adding latex microspheres modified by functional groups in the activation buffer;
[0081] (b) adding an activated cross-linking agent for activation;
[0082] (c) adding SAA antibody for cross-linking;
[0083] (d) Add latex blocking solution to block the residual activation sites.
[0084] Determination method of serum amyloid A
[0085] In the present invention, the assay method of serum amyloid A comprises the following steps:
[0086] (A) Use the serum amyloid A assay kit provided by the present invention to ...
Embodiment 1
[0104] The serum amyloid A assay kit of the present invention includes reagent R1 and reagent R2 two-liquid components that are independent of each other, wherein:
[0110] The serum amyloid A assay kit of the present invention includes reagent R1 and reagent R2 two-liquid components that are independent of each other, wherein:
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Abstract
The invention discloses a serum amyloid Aassay kit and a preparation method thereof. The kit includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein the reagent R1 component includes buffer solution 1 and reagent R2 Components include buffer 2 and SAA antibody latex beads. The serum amyloid Aassay kit of the invention has the advantages of simple operation, rapid response, high sensitivity and good specificity.
Description
technical field [0001] The invention relates to the field of medical immunodiagnosis, in particular to a serum amyloid Aassay kit and a preparation method thereof. Background technique [0002] In 1976, Rosenthal et al. isolated a highly heterogeneous protein with a relative molecular weight of about 12,000 and similar immunogenicity to tissue amyloid A from serum. Studies have shown that this substance is the precursor of tissue amyloid A. Therefore, this type of protein is called serum amyloid A (serum amyloid A, referred to as SAA). The synthetic gene for SAA is located on the short arm of chromosome 11 11p15.1 and spans 150Kd. SAA is mainly produced by hepatocytes, and also has constitutional expression in extrahepatic cells. When the body has an inflammatory response or an acute infection, interleukin (IL)-1 and tumor necrosis factor (TNF) released by macrophages synergistically increase the secretion of IL-6 within 48 to 72 hours, further stimulating the liver. Cel...
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