Kit for determining serum amyloid protein A and preparation method thereof
A technology of serum starch and kits, which is applied in the field of medical immunodiagnosis, and can solve the problems of cumbersome operations, fewer kits, and low product sensitivity
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[0075] Preparation method of serum amyloid A assay kit
[0076] In the present invention, the preparation method of the assay kit of serum amyloid A comprises the steps:
[0077] (A) prepare each component of R1 reagent, make R1 reagent;
[0078] (B) Prepare SAA antibody latex microspheres and other components of the R2 reagent to obtain the R2 reagent.
[0079] Wherein, the SAA antibody latex microspheres are prepared through the following steps:
[0080] (a) adding latex microspheres modified by functional groups in the activation buffer;
[0081] (b) adding an activated cross-linking agent for activation;
[0082] (c) adding SAA antibody for cross-linking;
[0083] (d) Add latex blocking solution to block the residual activation sites.
[0084] Determination method of serum amyloid A
[0085] In the present invention, the assay method of serum amyloid A comprises the following steps:
[0086] (A) Use the serum amyloid A assay kit provided by the present invention to ...
Embodiment 1
[0104] The serum amyloid A assay kit of the present invention includes reagent R1 and reagent R2 two-liquid components that are independent of each other, wherein:
[0105] Reagent R1 components include:
[0106]
[0107] Reagent R2 components include:
[0108]
Embodiment 2
[0110] The serum amyloid A assay kit of the present invention includes reagent R1 and reagent R2 two-liquid components that are independent of each other, wherein:
[0111] Reagent R1 components include:
[0112]
[0113]
[0114] Reagent R2 components include:
[0115]
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