46results about How to "Reduce steric effect" patented technology

The invention discloses a serum amyloid A assay kit and a preparation method thereof. The kit includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein the reagent R1 component includes buffer solution 1 and reagent R2 Components include buffer 2 and SAA antibody latex beads. The serum amyloid A assay kit of the invention has the advantages of simple operation, rapid response, high sensitivity and good specificity.

The present invention relates to protein chip based on labeling streptavidin-biotin technology. The hybridization reaction includes the following steps: dropping tested sample to sample applied chip and washing out redundant sample with elution liquid; blocking wtih blockage liquid, washing and air drying; dropping protein marked with biotin diluted by blockage liquid, washing and air drying; anddropping fluorescnet or enzyme labeled streptavidin diluted by blockage liquid, washing and air drying. The protein chip of the present invention is used in determining antigen and antibody, and detecting specific matter combined with protein. The improved method of the present invention has clear, stable and reliable test results and wide application range.

The present invention provides the preparation process of one kind of emulsifier for powdered emulsified explosive. The polymer emulsifier with excellent emulsifying performance and high emulsifying stability is prepared through the reaction between alkyl carboxylic acid or carboxylic anhydride and the mixture of several kinds of alcohol amine compounds. The powdered emulsified explosive prepared with the emulsifier has high explosion performance, high stability and long storing period.

The invention relates to a cardiac troponin I detection reagent, a preparation method and a cardiac troponin I detection kit. The cardiac troponin I detection reagent comprises a coated antibody solution, a detection antibody solution and an indicator solution, wherein the coated antibody comprises an antibody and stationary phase conjugate 1 obtained by coupling an antibody 1 with a stationary phase, and an antibody and stationary phase conjugate 2 obtained by coupling an antibody 2 with a stationary phase; the detection antibody is obtained by coupling an antibody 3 with a marker; and the indicator solution contains an indicator for quantitatively detecting the concentration of the marker. The invention also comprises the preparation method of the detection reagent and a kit using the detection reagent. In the invention, the antibody 2 and the antibody 3 can be respectively combined with the cardiac troponin I, so that the sufficient separation of the cardiac troponin I is facilitated, and the detection sensitivity of the reagent to the cardiac troponin I is further improved.

The invention provides a lithium ion battery electrode piece, a preparation method thereof, and a lithium ion battery, and belongs to the technical field of lithium ion batteries. The preparation method of the lithium ion battery electrode piece comprises the following steps: (1) slurry preparing; (2) slurry coating; (3) plasma etching: etching a dried electrode piece by adopting plasma etching equipment at room temperature, and forming a hole perpendicular to the surface of the electrode piece in the coating; (4) rolling and winding of the etched electrode piece for later use. The graphene, distributed in the horizontal direction, in the electrode piece is selectively etched through a plasma etching method, so that the transmission of lithium ions in the electrode piece is facilitated, the steric hindrance effect of the graphene is reduced, and the ionic conductivity of the lithium ion battery electrode piece is greatly enhanced.

Production of branched polyglycol consists of four steps: preparing branched polyglycol with glutamic acid of 9-fluorenmethoxy-oxo- protecting amino as central molecule, preparing branched polyglycol with glutamic acid as central molecule, preparing maleimide and preparing the final product. In the structural formula, n is >=1, and m is between 4-500. It can modify protein and polypeptide, decrease biological activity and hindering effect.

The invention provides ultra-high-molecular-weight polyether modified polysiloxane for a flatting agent and a preparation method and application of polyether modified polysiloxane. The preparation method of the polyether modified polysiloxane comprises the steps that allyl polyether and dichloromethylsilane are subjected to a hydrosilylation reaction in a solvent under catalysis of a platinum catalyst, and silyl ether is obtained; alkyl silane and obtained silyl ether are mixed and dropwise added to water for a hydrolysis reaction, and a hydrolysate is obtained; under catalysis of a basic catalyst, a cyclosiloxane monomer and the hydrolysate are subjected to a copolymerization reaction, and ultra-high-molecular-weight polyether modified polysiloxane is obtained through a neutral reaction.According to the preparation method, the smooth characteristic of ultra-high-molecular-weight polysiloxane is sufficiently used, the dispersibility of modified polysiloxane is excellent, and the smoothness and luster of water-based ink and paint are effectively improved; adopted polyether is easy to acquire, high in selectivity and low in cost.

The invention discloses a microcarrier for culturing animal cell and a crosslinking method thereof. The microcarrier is a microcarrier of ADCF which is synthesized by the following steps of taking Arginine or fully-synthesized Poly-Arginine as aglucon, and crosslinking the aglucon with the surface of dextran bead by taking allyl glycidyl ether as crosslinking activating agent. The microcarrier is polypeptide chain which is formed by polymerizing the arginine, has the property which is similar to animal gelatin, provides an adhering growing environment with ploy positive charge for the cell, and is good for the fast adhering and spreading of the cell and the high-intensity growing of the cell. The Arginine aglucon not only imitates the surface structure of the animal gelatin, but also does not induce animal-derived substances, and overcomes the problems of the quality and the safety of the product caused by inducing the animal-derived substances during large-scale cell culture.

A Cu(I)-Catalyzed Azide-Alkyne Cycloadditions (CuAAC) ligand comprising: a catalytic core; a fluorous tag; and a linker binding the fluorous tag to the catalytic core. A method for carrying out a Cu(I)-Catalyzed Azide-Alkyne Cycloaddition reaction, comprising: combining in a solution an alkyne-tagged component, an azide-tagged component and a Cu(I)-Catalyzed Azide-Alkyne Cycloadditions (CuAAC) ligand comprising: a catalytic core; a fluorous tag; and a linker binding the fluorous tag to the catalytic core; filtering the solution through a solid phase extraction filter to remove Cu(I)-ligand catalyst and / or excess ligand.

An unsaturated polyester resin preparation method comprises the following steps: proportioning according to the weight percent of each component, adding xytene-formaldehyde resin and saturated binary acid in a reaction kettle containing a thermometer, a mixer, a nitrogen ingress pipe and a condenser, heating gradually to 180-200 DEG C until the measured acid value is below 10mgKOH / g while mixing the mixture; reducing the temperature below 160 DEG C, then adding unsaturated binary acid, diol and partial polymerization inhibitor, heating gradually to 200-210 DEG C to react until the acid value is below 30mgKOH / g; reducing the temperature of the reaction products to 100 DEG C, adding residual polymerization inhibitor and crosslinkable monomer, and mixing the mixture for 1h to obtain the finished product. The invention has the advantages that the preparation method has simple process, environmentally friendly and no discharge of the three wastes; the steric effect of xytene-formaldehyde resin on double-bonds is reduced through the chain extension of long chain saturated binary acid, the product has stable quality, and excellent performances such as high reactivity, high strength, high toughness, high temperature tolerance and the like and the product can be widely used in chemical industry, metallurgy and other industries for preparing acid and base storage tanks, pickling tanks, plating bathes, waste water treatment pools, pipelines, terraces and the like.

The invention relates to an ethyl ester type fish oil refining processing technology, which specifically comprises: S1, pre-treating crude fish oil, wherein an adsorbent is added to crude fish oil, the crude fish oil is stirred and adsorbed by a thermomagnetic stirrer, thermal insulation stirring is performed for 30-40 min, the fish oil is cooled to a room temperature, the cooled fish oil is filtered to remove the adsorbent, and the obtained fish oil is used for spare; S2, carrying out a pre-esterification reaction, wherein the prepared fish oil is pumped into a reaction kettle, a uniformly-mixed mixture of an acid catalyst and ethanol is added to the reaction kettle, condensation water is introduced while stirring is performed, and the acid catalyst can catalyze the pre-esterification reaction of the fish oil under an ethanol reflux state to reduce the acid value of the fish oil; S3, carrying out an esterification reaction, wherein the fish oil obtained by the pre-esterification reaction in the step S2 is pumped into a reaction distillation tower, and an esterification reaction for fish oil refining and distillation concentration are synchronously performed in the reaction distillation tower, and the esterification reaction is an enzyme and alkali combined catalytic reaction; and S4, carrying out freeze decolorization and deoxidation on the fish oil prepared by the esterification reaction in the step S3 to obtain the ethyl ester type fish oil.

The invention discloses a recombinant protein A efficiently combined with IgG (Immunoglobulin G) and a construction method of an engineering bacterium thereof. The method comprises the following steps of: designing a recombinant protein A primer for IgG antibody combining areas E, D, A, B, C of a staphylococcal protein A (SPA) according to a gene sequence announced by the NCBI (National Center For Biotechnology Information), and adding a cysteine codon into an end C primer; amplifying by taking the genome of a staphylococcus aureus as a template to obtain a gene (SPA) of an encoding recombinant protein A antibody combining area, connecting the gene (SPA) with a plasmid pMD18-T, transforming competent E.coil JM109 to obtain a large quantity of recombinant plasmids pMD18-T-spa, storing the gene and increasing the copy numbers of the gene; and extracting the recombinant plasmids, performing double digestion on the recombinant plasmids and a plasmid pET-28a by using Noc I and BamH I, connecting a recombinant plasmid pET-28a-spa, transforming competent E.coil BL21, adding IPTG (Isopropyl beta-D-Thiogalactoside) of which the finial concentration is 1mmol / L for inducing expression at the temperature 30 DEG C, and identifying whether the expression is successful by using SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electropheresis).

The invention relates to a nylon material of a pin-type composite insulator cap-shaped connector for a power grid power transmission and distribution line and a forming method. The nylon material comprises the following components in percentage by mass: 62%-66% of nylon resin, 28%-33% of glass fiber, 3%-6% of a toughening agent, 0.2%-0.5% of a main antioxidant, 0.2%-0.5% of an auxiliary antioxidant, 0.2%-0.5% of an ultraviolet absorbent, 0.2%-0.5% of a light stabilizer and 1%-3% of a wear-resistant additive, the viscosity of the nylon resin is 3.2 + / -0.01; the grafting rate of the toughening agent is 0.8 to 1.2%; the nylon resin is PA66 polyhexamethylene adipamide, PA6 polyamide 6 or a mixture of the PA66 polyhexamethylene adipamide and the PA6 polyamide 6. After polymerization processing,the material performance is excellent, the mechanical performance, the electrical performance, the environmental aging resistance, the high and low environmental temperature resistance and the material wear resistance of the composite product are excellent, and the safe use condition of the product is met.

The invention provides a preparation method of polyacrylamide for an incense making adhesive. The preparation method comprises the following steps: S1, mixing acrylamide, acrylic acid, chitosan and deionized water to obtain a monomer solution A; S2, mixing methyl methacrylate, styrene, monomethyl fumarate and ethanol to obtain a monomer solution B; S3, mixing the monomer solution A and the monomersolution B, adding polyethylene and urea, and adjusting pH to be 5.5-6.5; S4, cooling to the temperature of 0-4 DEG C, introducing nitrogen and displacing oxygen, and then adding a DMAP catalyst anda chain extender; S5, heating to the temperature of 10-15 DEG C, and adding a cross-linking agent, a chain transfer agent, a reducing agent and an oxidizing agent into the mixture every 3 min; S6, heating to the temperature of 75-85 DEG C, carrying out heat-insulation reaction for 1-1.5 h, taking out an adhesive, adding caustic soda, hydrolyzing at the temperature of 80-90 DEG C for 1-2 h, and drying, crushing, granulating and carrying out secondary drying to obtain the final product. The polyacrylamide prepared by the invention has certain hydrophobic and water-resistant performance, the molecular weight reaches 30,000,000, and the polyacrylamide has the advantages of strong adhesive force, low moisture absorption rate, corrosion resistance and antibiosis, environmental protection and nopollution after combustion, and the like.

The invention belongs to the technical field of preparation methods of rigid polyurethane foam, and discloses a method for preparing rigid polyurethane foam from epoxidized lignin polyol. The method comprises the following steps: adding polyether polyol into a reactor, adding lignin polyol, uniformly stirring, sequentially adding a catalyst, a foam stabilizer and a foaming agent, mixing, uniformly stirring, finally adding quantitative isocyanate, quickly stirring, and freely foaming to obtain the lignin-based rigid polyurethane foam material. The hydroxyl value ratio of the lignin polyol to the polyether polyol is (2: 8)-(4: 6). According to the method for preparing the lignin-based rigid polyurethane foam material, side reactions are few, and the prepared lignin-based rigid polyurethane foam material is excellent in mechanical property, high in uniformity and regularity of the structure and high in thermal stability.

The invention belongs to the technical field of high-value utilization of lignin components in renewable resources, and relates to a preparation method of a lignin-based polyol. The method comprises the following steps: extracting corn straws, carrying out hydrothermal treatment on the extracted corn straws, carrying out alkali extraction on solid residues obtained after the hydrothermal treatment, and carrying out mild epoxidation modification reaction on alkali lignin obtained after the alkali extraction to finally obtain the product lignin-based polyol. The lignin-based polyol can be efficiently and mildly prepared from agricultural and forestry waste corn straw raw materials, so that high-value utilization of lignin components in renewable resources is realized.

The invention provides a preparation method of 1-(2,4-dichlorophenyl)-4-difluoromethyl-3-methyl-1H-1,2,4-triazole-5-one. The method comprises the steps: taking aniline as a raw material; reducing witha catalyst X to prepare phenylhydrazine; under the action of acetaldehyde and sodium cyanate, carrying out cyclization on phenylhydrazine to generate 1-phenyl-3-methyl-1H-1,2,4-triazole-5-one; carrying out a reaction on 1-phenyl-3-methyl-1H-1,2,4-triazole-5-one and monochlorodifluoromethane under the action of a catalyst Y to generate 1-phenyl-3-methyl-4-difluoromethyl-1H-1,2,4-triazole-5-one, and carrying out catalytic chlorination through a catalyst Z to prepare 1-(2,4-dichlorophenyl)-4-difluoromethyl-3-methyl-1H-1,2,4-triazole-5-one.

The invention belongs to the technical field of water treatment, and discloses a modification method for improving chlorine resistance of a polyacrylonitrile forward osmosis membrane. The method comprises the steps: dissolving polyacrylonitrile in an organic solvent N-methyl pyrrolidone, adding a pore-foaming agent, putting the mixture into an ultrasonic cleaning machine for degassing treatment after the mixture is completely dissolved, and removing gas generated in the stirring process to form a membrane casting solution; pouring the membrane casting solution on a glass plate, scraping the membrane by using a membrane coating device, then immediately putting the membrane into a deionized water coagulating bath, and obtaining a polyacrylonitrile support membrane by using an immersion-precipitation phase inversion method; and preparing a polyamide functional layer by virtue of an interfacial polymerization method, then cleaning with n-hexane, and removing unreacted 1,3,5-trimesoyl chloride on the surface, so as to obtain the cyclohexylamine modified polyacrylonitrile forward osmosis membrane. On the premise of ensuring the flux of the polyacrylonitrile forward osmosis membrane, the chlorine resistance of the membrane is greatly improved.

The invention discloses a corrosion inhibitor suitable for a hydrochloric acid system with a mass fraction of 20-28%. The corrosion inhibitor is composed of a quinoline quaternary ammonium salt, an imidazoline quaternary ammonium salt, alkynol, an acid-soluble metal salt, a low-molecular organic acid, a surfactant, an organic solvent and deionized water. The corrosion inhibitor is suitable for production increase operation construction of an ultra-high concentration hydrochloric acid system acidification oil and gas well, and has the characteristics of simple formula, good dissolution and dispersion, better corrosion inhibition performance and the like.

The invention discloses a click preparation method of an allyl cationic cyclodextrin chiral resolution material. The click preparation method comprises following steps: preparation of mono-6-(1-allylimidazole)-cyclodextrin; ion exchange of mono-6-(1-allylimidazole)-cyclodextrin; synthesis of mercapto-silica gel; click chemical reaction for preparation of an allyl cationic cyclodextrin chiral chromatographic separation material. Through the efficient thiol-ene click chemical reaction, mono-6-(1-allylimidazole) cyclodextrin is grafted to the mercapto modified porous silica gel surface, the novel natural allyl cationic cyclodextrin chiral chromatographic separation material is prepared, and an highly universal and effective path is provided for preparation of the chiral resolution material with a stable and controllable structure. The prepared allyl cationic cyclodextrin chiral resolution material can be used for chiral separation of compounds in a reverse chromatography mode, and the separation material has excellent separation capacity for different categories of drugs such as isoxazoline, flavanone, bendroflumethiazide, 4-chromanol and the like.

The invention belongs to a raw material for chemical production, provides a modified resorcinol formaldehyde resin free of pollution and spray frost and small in internal resistance and a production process thereof, and discloses a resorcinol aldehyde resin formed by the modification of olefin and aromatic hydrocarbon. The resorcinol aldehyde resin is prepared from the following components in parts by weight: 22 to 25 parts of resorcinol, 10 to 11.5 parts of aldehydes, 5 to 6.5 parts of olefines, 5 to 6.5 parts of aromatic hydrocarbons, 0.2 to 0.8 part of vitriol, wherein after all the components have an alkylation reaction and a hydroxylation reaction, the resorcinol aldehyde resin is prepared.

The invention relates to a method for synthesizing 4-chloro pyrrolo[2,3-b] pyridine. The method mainly comprises three steps: 1) carrying out p-toluenesulfonyl chloride protection on a 4-chloro pyrrolo[2,3-d]pyrimidine compound; 2) reacting the 4-chloro pyrrolo[2,3-d]pyrimidine compound protected by p-toluenesulfonyl chloride (Ts) with a compound to be substituted; and 3) carrying out Ts protecting group removal on the substituted compound obtained in the step 2 to obtain the target product. The 4-chloro pyrrolo[2,3-d]pyrimidine substituted compound obtained through a three-step chemical reaction process has a kinase inhibition effect, and is an important process for synthesizing a drug intermediate.

A process for creating a detection system of HBV DNA includes such steps as designing oligonucleotide probe by using the 1873-1912 sites of HBV DNA as target sequence, dividing the probe into two parts (one including 20 nucleotides and another including 6 nucleotides), using the paraffin thiol to decorate the 5' or 3' terminal of oligonucleotide, using Au-S bone to link to probe to Au or Fe3O4 / Au nanoparticle, using manipulated capture probe, and adding linking chain and signal amplifying substances in the detection system. Its advantages are high sensitivity and specificity and low cost.

The invention discloses a reusable Raman enhanced substrate, a preparation method and application thereof. The method comprises the following steps: ZnO seed crystal preparation; ZnO array preparation; ZnO / AuNPs substrate preparation; ZnO / Au / Ag 152 Preparation of 3D SERS substrates. Due to Ag 152 (SCH 2 CH 2 Ph) 60 The particle size of nanoclusters is extremely small, not only will not cover the hot spots on the original AuNPs film during the deposition process, but will further increase the hot spot density on its surface; greatly improving the SERS performance of the original substrate. The substrate of the present invention has good uniformity and signal reproducibility (RSD=6.95%), and can realize trace detection of analyte (5×10 ‑12 M), the enhancement factor can reach 6.48×10 9 , and can achieve cyclic detection. The method has the advantages of simple preparation process, low cost, high sensitivity, recyclable use and the like, and is suitable for mass production.

The invention provides an ultra-high molecular weight polyether-modified polysiloxane for a leveling agent and its preparation method and application. The preparation of the polyether-modified polysiloxane comprises the steps of: allyl polyether and methyl dimethicone Chlorosilane in a solvent, under the catalysis of a platinum catalyst, a hydrosilylation reaction occurs to obtain a silicon ether; the alkyl silane is mixed with the silicon ether obtained above, and added dropwise to water for a hydrolysis reaction to obtain a hydrolyzate; under the catalysis of a basic catalyst Under the action, the cyclosiloxane monomer and the hydrolyzate undergo a copolymerization reaction, and then it is obtained through a neutralization reaction. The invention makes full use of the slippery properties of ultra-high molecular weight polysiloxane, and has excellent dispersibility after modification, effectively improving the slipperiness and gloss of water-based inks and coatings; the polyether used is easy to obtain and has strong selectivity. low cost.

The invention provides a method for separating palmitoyl pentapeptide-4, which belongs to the technical field of polypeptide separation, and specifically includes: 1) placing the crude palmitoyl pentapeptide-4 in a dichloromethane / methanol mixed aqueous solution for microwave pretreatment, filtering 2) separation and purification by gel permeation chromatography; 3) separation and purification by reversed-phase high performance liquid chromatography twice; 4) freeze-drying to obtain high-purity palmitoyl pentapeptide-4. The palmitoyl pentapeptide-4 crude product obtained by solid-phase synthesis is separated and purified by the above method, the separation speed is fast, the sensitivity is high, and the resolution is strong, and most of the side reaction products and organic solvents in the crude peptide can be removed to obtain High-purity palmitoyl pentapeptide-4, with high separation and purification efficiency.

The invention discloses an extreme-pressure-resistant anti-wear agent and a preparation method thereof, and belongs to the field of lubricating oil additives. Tetraethyl silicate and the like are added into the prepared viscosity reducing active agent to form the viscosity reducing active agent with an acidic catalytic center, and the viscosity reducing active agent is modified by ammonium persulfate, so that the catalytic effect is enhanced, the viscosity of an oil base is reduced, the flowability is improved, and the wear resistance is improved; the nano additive prepared by the invention isoxidized by adding hydrogen peroxide and then is acidified, and soft metal is used for forming wear self-repairing on a friction interface, so that the nano additive has relatively high wear resistance; the interfacial polarity regulator prepared by the invention is matched with hexadecyl trimethyl ammonium bromide and isooctane to increase the solubility and reduce the surface tension, so that the anti-wear agent can well permeate into an interface, and the anti-wear efficiency is improved; the oil base viscosity is reduced through the viscosity reducing active agent, combustion is promoted,carbon deposition is reduced, and the effects of reducing energy consumption and prolonging the service life of the anti-wear agent are achieved. The anti-wear agent solves the problem that the existing anti-wear agent cannot reduce friction loss and oil consumption.

The invention discloses a concrete air entraining agent and a preparation method thereof and belongs to the field of concrete additives. According to the concrete air entraining agent, erucylamide, hydrochloric acid and the like are used as raw materials to synthesize a long-carbon-chain cationic surfactant to reduce the surface tension; then isopropanol is used as a solvent to react, and gas generated in a hydration process can be uniformly squeezed and entrained, so that the compactness is improved; polyaspartic acid is modified through fumaric acid and carboxylic acid and amide groups are introduced so that the air entraining efficiency is improved; and polyethylene vinyl acetate and polyvinyl versatate are added and can form a film when meeting water, so that the bending strength and permeability resistance of mortar are improved and the whole performance in an concrete air entraining process is improved. According to the concrete air entraining agent, foam is formed through the surfactant to close pores, so that the compactness and the water resisting performance are improved. By adopting the concrete air entraining agent, the problems of a current concrete air entraining agent that the foam stability is poor and the influence on the hardening strength of concrete is great are solved.